GSM2224570: Sox2 WT stomach 1 [RNA-seq]; Mus musculus; RNA-Seq

SRX1892800 GSM2224570 GSM2224570: Sox2 WT stomach 1 [RNA-seq]; Mus musculus; RNA-Seq SRP077732 SRS1539019 GSM2224570 RNA-Seq TRANSCRIPTOMIC cDNA Stomachs were laid out flat, cleaned, and pylorus was carefully excised. The pylorus was then placed in 5 mL of Corning Cell Recovery solution for 1 hour at 4 degrees. After this incubation, the muscle layer was gently separated from the epithelium/mesenchyme using fine tweezers. RNA was extracted from the epithelium/mesenchyme layer using the RNeasy kit from Qiagen according to their protocol. 1 ug of total RNA was used for the construction of the sequencing libraries RNA libraries were prepared for sequencing using standard Illumina protocols Illumina HiSeq 2500 gds 302224570 GEO Accession GSM2224570 SRX1892801 GSM2224571 GSM2224571: Sox2 WT stomach 2 [RNA-seq]; Mus musculus; RNA-Seq SRP077732 SRS1539018 GSM2224571 RNA-Seq TRANSCRIPTOMIC cDNA Stomachs were laid out flat, cleaned, and pylorus was carefully excised. The pylorus was then placed in 5 mL of Corning Cell Recovery solution for 1 hour at 4 degrees. After this incubation, the muscle layer was gently separated from the epithelium/mesenchyme using fine tweezers. RNA was extracted from the epithelium/mesenchyme layer using the RNeasy kit from Qiagen according to their protocol. 1 ug of total RNA was used for the construction of the sequencing libraries RNA libraries were prepared for sequencing using standard Illumina protocols Illumina HiSeq 2500 gds 302224571 GEO Accession GSM2224571 SRX1892802 GSM2224572 GSM2224572: Sox2 WT stomach 3 [RNA-seq]; Mus musculus; RNA-Seq SRP077732 SRS1539017 GSM2224572 RNA-Seq TRANSCRIPTOMIC cDNA Stomachs were laid out flat, cleaned, and pylorus was carefully excised. The pylorus was then placed in 5 mL of Corning Cell Recovery solution for 1 hour at 4 degrees. After this incubation, the muscle layer was gently separated from the epithelium/mesenchyme using fine tweezers. RNA was extracted from the epithelium/mesenchyme layer using the RNeasy kit from Qiagen according to their protocol. 1 ug of total RNA was used for the construction of the sequencing libraries RNA libraries were prepared for sequencing using standard Illumina protocols Illumina HiSeq 2500 gds 302224572 GEO Accession GSM2224572 SRX1892803 GSM2224573 GSM2224573: Sox2 KO stomach 1 [RNA-seq]; Mus musculus; RNA-Seq SRP077732 SRS1539024 GSM2224573 RNA-Seq TRANSCRIPTOMIC cDNA Stomachs were laid out flat, cleaned, and pylorus was carefully excised. The pylorus was then placed in 5 mL of Corning Cell Recovery solution for 1 hour at 4 degrees. After this incubation, the muscle layer was gently separated from the epithelium/mesenchyme using fine tweezers. RNA was extracted from the epithelium/mesenchyme layer using the RNeasy kit from Qiagen according to their protocol. 1 ug of total RNA was used for the construction of the sequencing libraries RNA libraries were prepared for sequencing using standard Illumina protocols Illumina HiSeq 2500 gds 302224573 GEO Accession GSM2224573 SRX1892804 GSM2224574 GSM2224574: Sox2 KO stomach 2 [RNA-seq]; Mus musculus; RNA-Seq SRP077732 SRS1539023 GSM2224574 RNA-Seq TRANSCRIPTOMIC cDNA Stomachs were laid out flat, cleaned, and pylorus was carefully excised. The pylorus was then placed in 5 mL of Corning Cell Recovery solution for 1 hour at 4 degrees. After this incubation, the muscle layer was gently separated from the epithelium/mesenchyme using fine tweezers. RNA was extracted from the epithelium/mesenchyme layer using the RNeasy kit from Qiagen according to their protocol. 1 ug of total RNA was used for the construction of the sequencing libraries RNA libraries were prepared for sequencing using standard Illumina protocols Illumina HiSeq 2500 gds 302224574 GEO Accession GSM2224574 SRX1892805 GSM2224575 GSM2224575: Sox2 KO stomach 3 [RNA-seq]; Mus musculus; RNA-Seq SRP077732 SRS1539022 GSM2224575 RNA-Seq TRANSCRIPTOMIC cDNA Stomachs were laid out flat, cleaned, and pylorus was carefully excised. The pylorus was then placed in 5 mL of Corning Cell Recovery solution for 1 hour at 4 degrees. After this incubation, the muscle layer was gently separated from the epithelium/mesenchyme using fine tweezers. RNA was extracted from the epithelium/mesenchyme layer using the RNeasy kit from Qiagen according to their protocol. 1 ug of total RNA was used for the construction of the sequencing libraries RNA libraries were prepared for sequencing using standard Illumina protocols Illumina HiSeq 2500 gds 302224575 GEO Accession GSM2224575