<?xml version="1.0" encoding="UTF-8"?>
<EXPERIMENT_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <EXPERIMENT accession="SRX1896592" alias="206.1.1plaB4.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896592</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">206.1.1plaB4.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539612">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539612</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335457</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>206.1.1plaB4.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
      <SPOT_DESCRIPTOR>
        <SPOT_DECODE_SPEC>
          <SPOT_LENGTH>100</SPOT_LENGTH>
          <READ_SPEC>
            <READ_INDEX>0</READ_INDEX>
            <READ_CLASS>Application Read</READ_CLASS>
            <READ_TYPE>Forward</READ_TYPE>
            <BASE_COORD>1</BASE_COORD>
          </READ_SPEC>
        </SPOT_DECODE_SPEC>
      </SPOT_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896593" alias="260.1.1plaB3.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896593</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">260.1.1plaB3.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539613">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539613</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335458</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>260.1.1plaB3.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896594" alias="671.2plaB7.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896594</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">671.2plaB7.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539614">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539614</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335467</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>671.2plaB7.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
      <SPOT_DESCRIPTOR>
        <SPOT_DECODE_SPEC>
          <SPOT_LENGTH>100</SPOT_LENGTH>
          <READ_SPEC>
            <READ_INDEX>0</READ_INDEX>
            <READ_CLASS>Application Read</READ_CLASS>
            <READ_TYPE>Forward</READ_TYPE>
            <BASE_COORD>1</BASE_COORD>
          </READ_SPEC>
        </SPOT_DECODE_SPEC>
      </SPOT_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896595" alias="671.dry.2colB7.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896595</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">671.dry.2colB7.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539615">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539615</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335468</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>671.dry.2colB7.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896596" alias="705.3.1plaB28.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896596</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">705.3.1plaB28.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539616">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539616</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335469</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>705.3.1plaB28.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896597" alias="750.1plaB11b.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896597</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">750.1plaB11b.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539617">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539617</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335470</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>750.1plaB11b.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896598" alias="750.2plaB11b.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896598</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">750.2plaB11b.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539618">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539618</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335471</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>750.2plaB11b.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896599" alias="750.dry.2colB11b.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896599</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">750.dry.2colB11b.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539619">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539619</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335472</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>750.dry.2colB11b.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896600" alias="770.3.2plaB3.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896600</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">770.3.2plaB3.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539620">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539620</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335473</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>770.3.2plaB3.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896601" alias="782.1plaB15.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896601</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">782.1plaB15.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539621">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539621</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335474</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>782.1plaB15.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896602" alias="820.1plaB25.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896602</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">820.1plaB25.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539622">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539622</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335475</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>820.1plaB25.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896603" alias="821.1plaB17.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896603</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">821.1plaB17.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539623">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539623</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335476</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>821.1plaB17.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896604" alias="263.1plaB6.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896604</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">263.1plaB6.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539624">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539624</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335459</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>263.1plaB6.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896605" alias="896.1plaB14.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896605</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">896.1plaB14.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539625">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539625</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335477</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>896.1plaB14.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896606" alias="896.2plaB14.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896606</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">896.2plaB14.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539626">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539626</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335478</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>896.2plaB14.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896607" alias="896.dry.2colB14.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896607</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">896.dry.2colB14.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539627">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539627</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335479</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>896.dry.2colB14.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896608" alias="263.2plaB6.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896608</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">263.2plaB6.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539628">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539628</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335460</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>263.2plaB6.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896610" alias="263.dry.2colB6.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896610</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">263.dry.2colB6.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539630">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539630</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335461</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>263.dry.2colB6.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896611" alias="271.1plaB13.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896611</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">271.1plaB13.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539629">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539629</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335462</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>271.1plaB13.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896612" alias="328.1plaB12b.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896612</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">328.1plaB12b.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539632">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539632</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335463</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>328.1plaB12b.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896613" alias="58.1plaB10.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896613</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">58.1plaB10.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539631">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539631</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335464</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>58.1plaB10.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896614" alias="652.1plaB9.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896614</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">652.1plaB9.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539633">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539633</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335465</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>652.1plaB9.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX1896615" alias="671.1plaB7.Y2011">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX1896615</PRIMARY_ID>
      <SUBMITTER_ID namespace="SUB1660853">671.1plaB7.Y2011</SUBMITTER_ID>
    </IDENTIFIERS>
    <TITLE>16S rRNA gene of Bacteria: Western Swiss Alps grassland soil</TITLE>
    <STUDY_REF accession="SRP077863">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP077863</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA327018</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION>Grassland soil was collected from the western Swiss Alps study area. The total community DNA was extracted from soil samples using the MoBio PowerSoil DNA extraction kit in triplicate and combined. PCR of the V5 region of the bacterial 16S rRNA gene was done in 4x using barcoded 784DEG and 880RDEG primers, and replicate PCR products were combined prior to purification with MinElute. Paired-end 100bp sequencing was done on the HiSEQ Illumina platform. The primer barcodes were removed, and PandaSeq was used to obtain consensus reads from each paired-end reads.</DESIGN_DESCRIPTION>
      <SAMPLE_DESCRIPTOR accession="SRS1539634">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS1539634</PRIMARY_ID>
          <EXTERNAL_ID namespace="BioSample">SAMN05335466</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>671.1plaB7.Y2011</LIBRARY_NAME>
        <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>Illumina HiSeq 2500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
</EXPERIMENT_SET>
