SRX1985067 GSM2254593 SRP080128 SRS1590196 GSM2254593 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from 1 g tissue from each. Each sample was mechanically ground in liquid nitrogen until a fine powder was obtained. To this tissue 3 % insoluble polyvinylpyrrolidinone (PVPP) was added and further ground in an extraction buffer containing 2% hexadecyltrimethylammonium bromide (CTAB), 2.0 M sodium chloride, 0.5 g/L spermidine, 100 mM Tris-HCl, 25 mM EDTA and 3% ß-mercaptoethanol pre-warmed to 65 °C. The mixture was then incubated at 65 °C for 10 minutes before extraction with 24:1 chloroform:isoamyl alcohol. The RNA was precipitated by the addition of ¼ volume 10 M LiCl, incubation at 4 °C for 6 hours followed by incubation at -20 °C overnight. The RNA was then harvested by centrifugation (10 000 x g) at 4 °C for one hour, removing aqueous phase, and washing in 70 % ethanol. Pellets were dried, re-dissolved in RNase- free water, and stored at -80 °C until use. Unstranded RNA protocol Illumina HiSeq 2000 gds 302254593 GEO Accession GSM2254593 SRX1985068 GSM2254594 SRP080128 SRS1590197 GSM2254594 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from 1 g tissue from each. Each sample was mechanically ground in liquid nitrogen until a fine powder was obtained. To this tissue 3 % insoluble polyvinylpyrrolidinone (PVPP) was added and further ground in an extraction buffer containing 2% hexadecyltrimethylammonium bromide (CTAB), 2.0 M sodium chloride, 0.5 g/L spermidine, 100 mM Tris-HCl, 25 mM EDTA and 3% ß-mercaptoethanol pre-warmed to 65 °C. The mixture was then incubated at 65 °C for 10 minutes before extraction with 24:1 chloroform:isoamyl alcohol. The RNA was precipitated by the addition of ¼ volume 10 M LiCl, incubation at 4 °C for 6 hours followed by incubation at -20 °C overnight. The RNA was then harvested by centrifugation (10 000 x g) at 4 °C for one hour, removing aqueous phase, and washing in 70 % ethanol. Pellets were dried, re-dissolved in RNase- free water, and stored at -80 °C until use. Unstranded RNA protocol Illumina HiSeq 2000 gds 302254594 GEO Accession GSM2254594 SRX1985069 GSM2254595 SRP080128 SRS1590198 GSM2254595 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from 1 g tissue from each. Each sample was mechanically ground in liquid nitrogen until a fine powder was obtained. To this tissue 3 % insoluble polyvinylpyrrolidinone (PVPP) was added and further ground in an extraction buffer containing 2% hexadecyltrimethylammonium bromide (CTAB), 2.0 M sodium chloride, 0.5 g/L spermidine, 100 mM Tris-HCl, 25 mM EDTA and 3% ß-mercaptoethanol pre-warmed to 65 °C. The mixture was then incubated at 65 °C for 10 minutes before extraction with 24:1 chloroform:isoamyl alcohol. The RNA was precipitated by the addition of ¼ volume 10 M LiCl, incubation at 4 °C for 6 hours followed by incubation at -20 °C overnight. The RNA was then harvested by centrifugation (10 000 x g) at 4 °C for one hour, removing aqueous phase, and washing in 70 % ethanol. Pellets were dried, re-dissolved in RNase- free water, and stored at -80 °C until use. Unstranded RNA protocol Illumina HiSeq 2000 gds 302254595 GEO Accession GSM2254595 SRX1985070 GSM2254596 SRP080128 SRS1590199 GSM2254596 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from 1 g tissue from each. Each sample was mechanically ground in liquid nitrogen until a fine powder was obtained. To this tissue 3 % insoluble polyvinylpyrrolidinone (PVPP) was added and further ground in an extraction buffer containing 2% hexadecyltrimethylammonium bromide (CTAB), 2.0 M sodium chloride, 0.5 g/L spermidine, 100 mM Tris-HCl, 25 mM EDTA and 3% ß-mercaptoethanol pre-warmed to 65 °C. The mixture was then incubated at 65 °C for 10 minutes before extraction with 24:1 chloroform:isoamyl alcohol. The RNA was precipitated by the addition of ¼ volume 10 M LiCl, incubation at 4 °C for 6 hours followed by incubation at -20 °C overnight. The RNA was then harvested by centrifugation (10 000 x g) at 4 °C for one hour, removing aqueous phase, and washing in 70 % ethanol. Pellets were dried, re-dissolved in RNase- free water, and stored at -80 °C until use. Unstranded RNA protocol Illumina HiSeq 2000 gds 302254596 GEO Accession GSM2254596 SRX1985071 GSM2254597 SRP080128 SRS1590200 GSM2254597 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from 1 g tissue from each. Each sample was mechanically ground in liquid nitrogen until a fine powder was obtained. To this tissue 3 % insoluble polyvinylpyrrolidinone (PVPP) was added and further ground in an extraction buffer containing 2% hexadecyltrimethylammonium bromide (CTAB), 2.0 M sodium chloride, 0.5 g/L spermidine, 100 mM Tris-HCl, 25 mM EDTA and 3% ß-mercaptoethanol pre-warmed to 65 °C. The mixture was then incubated at 65 °C for 10 minutes before extraction with 24:1 chloroform:isoamyl alcohol. The RNA was precipitated by the addition of ¼ volume 10 M LiCl, incubation at 4 °C for 6 hours followed by incubation at -20 °C overnight. The RNA was then harvested by centrifugation (10 000 x g) at 4 °C for one hour, removing aqueous phase, and washing in 70 % ethanol. Pellets were dried, re-dissolved in RNase- free water, and stored at -80 °C until use. Unstranded RNA protocol Illumina HiSeq 2000 gds 302254597 GEO Accession GSM2254597 SRX1985072 GSM2254598 SRP080128 SRS1590201 GSM2254598 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from 1 g tissue from each. Each sample was mechanically ground in liquid nitrogen until a fine powder was obtained. To this tissue 3 % insoluble polyvinylpyrrolidinone (PVPP) was added and further ground in an extraction buffer containing 2% hexadecyltrimethylammonium bromide (CTAB), 2.0 M sodium chloride, 0.5 g/L spermidine, 100 mM Tris-HCl, 25 mM EDTA and 3% ß-mercaptoethanol pre-warmed to 65 °C. The mixture was then incubated at 65 °C for 10 minutes before extraction with 24:1 chloroform:isoamyl alcohol. The RNA was precipitated by the addition of ¼ volume 10 M LiCl, incubation at 4 °C for 6 hours followed by incubation at -20 °C overnight. The RNA was then harvested by centrifugation (10 000 x g) at 4 °C for one hour, removing aqueous phase, and washing in 70 % ethanol. Pellets were dried, re-dissolved in RNase- free water, and stored at -80 °C until use. Unstranded RNA protocol Illumina HiSeq 2000 gds 302254598 GEO Accession GSM2254598 SRX1985073 GSM2254599 SRP080128 SRS1590202 GSM2254599 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from 1 g tissue from each. Each sample was mechanically ground in liquid nitrogen until a fine powder was obtained. To this tissue 3 % insoluble polyvinylpyrrolidinone (PVPP) was added and further ground in an extraction buffer containing 2% hexadecyltrimethylammonium bromide (CTAB), 2.0 M sodium chloride, 0.5 g/L spermidine, 100 mM Tris-HCl, 25 mM EDTA and 3% ß-mercaptoethanol pre-warmed to 65 °C. The mixture was then incubated at 65 °C for 10 minutes before extraction with 24:1 chloroform:isoamyl alcohol. The RNA was precipitated by the addition of ¼ volume 10 M LiCl, incubation at 4 °C for 6 hours followed by incubation at -20 °C overnight. The RNA was then harvested by centrifugation (10 000 x g) at 4 °C for one hour, removing aqueous phase, and washing in 70 % ethanol. Pellets were dried, re-dissolved in RNase- free water, and stored at -80 °C until use. Unstranded RNA protocol Illumina HiSeq 2000 gds 302254599 GEO Accession GSM2254599 SRX1985074 GSM2254600 SRP080128 SRS1590203 GSM2254600 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from 1 g tissue from each. Each sample was mechanically ground in liquid nitrogen until a fine powder was obtained. To this tissue 3 % insoluble polyvinylpyrrolidinone (PVPP) was added and further ground in an extraction buffer containing 2% hexadecyltrimethylammonium bromide (CTAB), 2.0 M sodium chloride, 0.5 g/L spermidine, 100 mM Tris-HCl, 25 mM EDTA and 3% ß-mercaptoethanol pre-warmed to 65 °C. The mixture was then incubated at 65 °C for 10 minutes before extraction with 24:1 chloroform:isoamyl alcohol. The RNA was precipitated by the addition of ¼ volume 10 M LiCl, incubation at 4 °C for 6 hours followed by incubation at -20 °C overnight. The RNA was then harvested by centrifugation (10 000 x g) at 4 °C for one hour, removing aqueous phase, and washing in 70 % ethanol. Pellets were dried, re-dissolved in RNase- free water, and stored at -80 °C until use. Unstranded RNA protocol Illumina HiSeq 2000 gds 302254600 GEO Accession GSM2254600 SRX1985075 GSM2254601 SRP080128 SRS1590204 GSM2254601 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from 1 g tissue from each. Each sample was mechanically ground in liquid nitrogen until a fine powder was obtained. To this tissue 3 % insoluble polyvinylpyrrolidinone (PVPP) was added and further ground in an extraction buffer containing 2% hexadecyltrimethylammonium bromide (CTAB), 2.0 M sodium chloride, 0.5 g/L spermidine, 100 mM Tris-HCl, 25 mM EDTA and 3% ß-mercaptoethanol pre-warmed to 65 °C. The mixture was then incubated at 65 °C for 10 minutes before extraction with 24:1 chloroform:isoamyl alcohol. The RNA was precipitated by the addition of ¼ volume 10 M LiCl, incubation at 4 °C for 6 hours followed by incubation at -20 °C overnight. The RNA was then harvested by centrifugation (10 000 x g) at 4 °C for one hour, removing aqueous phase, and washing in 70 % ethanol. Pellets were dried, re-dissolved in RNase- free water, and stored at -80 °C until use. Unstranded RNA protocol Illumina HiSeq 2000 gds 302254601 GEO Accession GSM2254601 SRX1985076 GSM2254602 SRP080128 SRS1590205 GSM2254602 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from 1 g tissue from each. Each sample was mechanically ground in liquid nitrogen until a fine powder was obtained. To this tissue 3 % insoluble polyvinylpyrrolidinone (PVPP) was added and further ground in an extraction buffer containing 2% hexadecyltrimethylammonium bromide (CTAB), 2.0 M sodium chloride, 0.5 g/L spermidine, 100 mM Tris-HCl, 25 mM EDTA and 3% ß-mercaptoethanol pre-warmed to 65 °C. The mixture was then incubated at 65 °C for 10 minutes before extraction with 24:1 chloroform:isoamyl alcohol. The RNA was precipitated by the addition of ¼ volume 10 M LiCl, incubation at 4 °C for 6 hours followed by incubation at -20 °C overnight. The RNA was then harvested by centrifugation (10 000 x g) at 4 °C for one hour, removing aqueous phase, and washing in 70 % ethanol. Pellets were dried, re-dissolved in RNase- free water, and stored at -80 °C until use. Unstranded RNA protocol Illumina HiSeq 2000 gds 302254602 GEO Accession GSM2254602 SRX1985077 GSM2254603 SRP080128 SRS1590206 GSM2254603 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from 1 g tissue from each. Each sample was mechanically ground in liquid nitrogen until a fine powder was obtained. To this tissue 3 % insoluble polyvinylpyrrolidinone (PVPP) was added and further ground in an extraction buffer containing 2% hexadecyltrimethylammonium bromide (CTAB), 2.0 M sodium chloride, 0.5 g/L spermidine, 100 mM Tris-HCl, 25 mM EDTA and 3% ß-mercaptoethanol pre-warmed to 65 °C. The mixture was then incubated at 65 °C for 10 minutes before extraction with 24:1 chloroform:isoamyl alcohol. The RNA was precipitated by the addition of ¼ volume 10 M LiCl, incubation at 4 °C for 6 hours followed by incubation at -20 °C overnight. The RNA was then harvested by centrifugation (10 000 x g) at 4 °C for one hour, removing aqueous phase, and washing in 70 % ethanol. Pellets were dried, re-dissolved in RNase- free water, and stored at -80 °C until use. Unstranded RNA protocol Illumina HiSeq 2000 gds 302254603 GEO Accession GSM2254603 SRX1985078 GSM2254604 SRP080128 SRS1590207 GSM2254604 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from 1 g tissue from each. Each sample was mechanically ground in liquid nitrogen until a fine powder was obtained. To this tissue 3 % insoluble polyvinylpyrrolidinone (PVPP) was added and further ground in an extraction buffer containing 2% hexadecyltrimethylammonium bromide (CTAB), 2.0 M sodium chloride, 0.5 g/L spermidine, 100 mM Tris-HCl, 25 mM EDTA and 3% ß-mercaptoethanol pre-warmed to 65 °C. The mixture was then incubated at 65 °C for 10 minutes before extraction with 24:1 chloroform:isoamyl alcohol. The RNA was precipitated by the addition of ¼ volume 10 M LiCl, incubation at 4 °C for 6 hours followed by incubation at -20 °C overnight. The RNA was then harvested by centrifugation (10 000 x g) at 4 °C for one hour, removing aqueous phase, and washing in 70 % ethanol. Pellets were dried, re-dissolved in RNase- free water, and stored at -80 °C until use. Unstranded RNA protocol Illumina HiSeq 2000 gds 302254604 GEO Accession GSM2254604 SRX1985079 GSM2254605 SRP080128 SRS1590208 GSM2254605 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from 1 g tissue from each. Each sample was mechanically ground in liquid nitrogen until a fine powder was obtained. To this tissue 3 % insoluble polyvinylpyrrolidinone (PVPP) was added and further ground in an extraction buffer containing 2% hexadecyltrimethylammonium bromide (CTAB), 2.0 M sodium chloride, 0.5 g/L spermidine, 100 mM Tris-HCl, 25 mM EDTA and 3% ß-mercaptoethanol pre-warmed to 65 °C. The mixture was then incubated at 65 °C for 10 minutes before extraction with 24:1 chloroform:isoamyl alcohol. The RNA was precipitated by the addition of ¼ volume 10 M LiCl, incubation at 4 °C for 6 hours followed by incubation at -20 °C overnight. The RNA was then harvested by centrifugation (10 000 x g) at 4 °C for one hour, removing aqueous phase, and washing in 70 % ethanol. Pellets were dried, re-dissolved in RNase- free water, and stored at -80 °C until use. Unstranded RNA protocol Illumina HiSeq 2000 gds 302254605 GEO Accession GSM2254605 SRX1985080 GSM2254606 SRP080128 SRS1590209 GSM2254606 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from 1 g tissue from each. Each sample was mechanically ground in liquid nitrogen until a fine powder was obtained. To this tissue 3 % insoluble polyvinylpyrrolidinone (PVPP) was added and further ground in an extraction buffer containing 2% hexadecyltrimethylammonium bromide (CTAB), 2.0 M sodium chloride, 0.5 g/L spermidine, 100 mM Tris-HCl, 25 mM EDTA and 3% ß-mercaptoethanol pre-warmed to 65 °C. The mixture was then incubated at 65 °C for 10 minutes before extraction with 24:1 chloroform:isoamyl alcohol. The RNA was precipitated by the addition of ¼ volume 10 M LiCl, incubation at 4 °C for 6 hours followed by incubation at -20 °C overnight. The RNA was then harvested by centrifugation (10 000 x g) at 4 °C for one hour, removing aqueous phase, and washing in 70 % ethanol. Pellets were dried, re-dissolved in RNase- free water, and stored at -80 °C until use. Unstranded RNA protocol Illumina HiSeq 2000 gds 302254606 GEO Accession GSM2254606