SRX2059024 1234 SRP083100 PRJNA338374 The IlluminaŐs TruSeq indexed pair-ended DNA library preparation protocol was performed automatically on the SPRIworks system (Beckman Coulter, Inc., Brea CA). By using cartridge and method card specific to Illumina sequencing system, fragment library can be prepared for Illumina sequencers. After individual libraries were constructed, qualities and band-sizes were assessed using Bioanalyzer High Sensitivity Chip (Agilent Technologies, CA) and Qubit (Life Technologies, CA). Libraries were also quantified by qPCR using the Library Quantification Kit for Illumina sequencing platforms (KAPA Biosystems, Boston, USA), using an ABI 7900HT Real-Time PCR System (Life Technologies). Libraries were normalized to a working concentration of 10 nM, using the molarity calculated from qPCR and adjusted for fragment size with the Bioanalyzer analysis. SRS1650992 SAMN05545220 1234 WGS GENOMIC RT-PCR Illumina HiSeq 2000