SRX2169702 8075_WT_NOD_TTAGGC SRP089972 PRJNA342340 otal RNAs from each mouse (pooled of three uterine nodules from same mouse) were isolated using TRIzol and cleaned up using RNeasy Mini Kit (Qiagen), and RNAs quality were determined using a 2100 Bioanalyzer (agilent) to make sure RIN number is above 8. RNA libraries were prepared using the TruSeq Stranded mRNA library Preparation Kit (Illumina). Briefly, RNAs were reverse transcripted to cDNAs and adapters with uniq barcode were ligated to RNA libraries from mouse. The libraries were then pooled in eauimolar amounts. The pool was then loaded on two lanes of an Illumina HiSeq 2500 Rapid Run flow cell (v1) and sequenced using TruSeq Rapid SBS Reagents in a 1x50bp single read format. Base calling was done by Illumina Real Time Analysis (RTA) v1.18.64 and output of RTA demultiplexed and converted to FastQ format with Illumina Bcl2fastq v1.8.4. SRS1697599 SAMN05755754 8075_WT_NOD_TTAGGC RNA-Seq TRANSCRIPTOMIC RT-PCR Illumina HiSeq 2500 SRX2169703 8082_WT_NOD_GATCAG SRP089972 PRJNA342340 otal RNAs from each mouse (pooled of three uterine nodules from same mouse) were isolated using TRIzol and cleaned up using RNeasy Mini Kit (Qiagen), and RNAs quality were determined using a 2100 Bioanalyzer (agilent) to make sure RIN number is above 8. RNA libraries were prepared using the TruSeq Stranded mRNA library Preparation Kit (Illumina). Briefly, RNAs were reverse transcripted to cDNAs and adapters with uniq barcode were ligated to RNA libraries from mouse. The libraries were then pooled in eauimolar amounts. The pool was then loaded on two lanes of an Illumina HiSeq 2500 Rapid Run flow cell (v1) and sequenced using TruSeq Rapid SBS Reagents in a 1x50bp single read format. Base calling was done by Illumina Real Time Analysis (RTA) v1.18.64 and output of RTA demultiplexed and converted to FastQ format with Illumina Bcl2fastq v1.8.4. SRS1697599 SAMN05755754 8082_WT_NOD_GATCAG RNA-Seq TRANSCRIPTOMIC RT-PCR Illumina HiSeq 2500 SRX2169704 8119_WT_NOD_GGCTAC SRP089972 PRJNA342340 otal RNAs from each mouse (pooled of three uterine nodules from same mouse) were isolated using TRIzol and cleaned up using RNeasy Mini Kit (Qiagen), and RNAs quality were determined using a 2100 Bioanalyzer (agilent) to make sure RIN number is above 8. RNA libraries were prepared using the TruSeq Stranded mRNA library Preparation Kit (Illumina). Briefly, RNAs were reverse transcripted to cDNAs and adapters with uniq barcode were ligated to RNA libraries from mouse. The libraries were then pooled in eauimolar amounts. The pool was then loaded on two lanes of an Illumina HiSeq 2500 Rapid Run flow cell (v1) and sequenced using TruSeq Rapid SBS Reagents in a 1x50bp single read format. Base calling was done by Illumina Real Time Analysis (RTA) v1.18.64 and output of RTA demultiplexed and converted to FastQ format with Illumina Bcl2fastq v1.8.4. SRS1697599 SAMN05755754 8119_WT_NOD_GGCTAC RNA-Seq TRANSCRIPTOMIC RT-PCR Illumina HiSeq 2500 SRX2169705 8153_KO_NOD_GTTTCG SRP089972 PRJNA342340 otal RNAs from each mouse (pooled of three uterine nodules from same mouse) were isolated using TRIzol and cleaned up using RNeasy Mini Kit (Qiagen), and RNAs quality were determined using a 2100 Bioanalyzer (agilent) to make sure RIN number is above 8. RNA libraries were prepared using the TruSeq Stranded mRNA library Preparation Kit (Illumina). Briefly, RNAs were reverse transcripted to cDNAs and adapters with uniq barcode were ligated to RNA libraries from mouse. The libraries were then pooled in eauimolar amounts. The pool was then loaded on two lanes of an Illumina HiSeq 2500 Rapid Run flow cell (v1) and sequenced using TruSeq Rapid SBS Reagents in a 1x50bp single read format. Base calling was done by Illumina Real Time Analysis (RTA) v1.18.64 and output of RTA demultiplexed and converted to FastQ format with Illumina Bcl2fastq v1.8.4. SRS1697599 SAMN05755754 8153_KO_NOD_GTTTCG RNA-Seq TRANSCRIPTOMIC RT-PCR Illumina HiSeq 2500 SRX2169706 8154_KO_NOD_GAGTGG SRP089972 PRJNA342340 otal RNAs from each mouse (pooled of three uterine nodules from same mouse) were isolated using TRIzol and cleaned up using RNeasy Mini Kit (Qiagen), and RNAs quality were determined using a 2100 Bioanalyzer (agilent) to make sure RIN number is above 8. RNA libraries were prepared using the TruSeq Stranded mRNA library Preparation Kit (Illumina). Briefly, RNAs were reverse transcripted to cDNAs and adapters with uniq barcode were ligated to RNA libraries from mouse. The libraries were then pooled in eauimolar amounts. The pool was then loaded on two lanes of an Illumina HiSeq 2500 Rapid Run flow cell (v1) and sequenced using TruSeq Rapid SBS Reagents in a 1x50bp single read format. Base calling was done by Illumina Real Time Analysis (RTA) v1.18.64 and output of RTA demultiplexed and converted to FastQ format with Illumina Bcl2fastq v1.8.4. SRS1697599 SAMN05755754 8154_KO_NOD_GAGTGG RNA-Seq TRANSCRIPTOMIC RT-PCR Illumina HiSeq 2500 SRX2169707 8177_KO_NOD_ATTCCT SRP089972 PRJNA342340 otal RNAs from each mouse (pooled of three uterine nodules from same mouse) were isolated using TRIzol and cleaned up using RNeasy Mini Kit (Qiagen), and RNAs quality were determined using a 2100 Bioanalyzer (agilent) to make sure RIN number is above 8. RNA libraries were prepared using the TruSeq Stranded mRNA library Preparation Kit (Illumina). Briefly, RNAs were reverse transcripted to cDNAs and adapters with uniq barcode were ligated to RNA libraries from mouse. The libraries were then pooled in eauimolar amounts. The pool was then loaded on two lanes of an Illumina HiSeq 2500 Rapid Run flow cell (v1) and sequenced using TruSeq Rapid SBS Reagents in a 1x50bp single read format. Base calling was done by Illumina Real Time Analysis (RTA) v1.18.64 and output of RTA demultiplexed and converted to FastQ format with Illumina Bcl2fastq v1.8.4. SRS1697599 SAMN05755754 8177_KO_NOD_ATTCCT RNA-Seq TRANSCRIPTOMIC RT-PCR Illumina HiSeq 2500