SRX2178811 GSM2322580 SRP090167 SRS1704188 GSM2322580 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was isolated from cells using Trizol (Invitrogen, USA) according to manufacturer’s instructions. RNA cleanup involved DNase I digestion step was performed using RAeasy spin colmna (Qiagen), followed by assessment of RNA purity using the ND-1000 Nanodrop. Purified RNA samples had an A260:A230 ratio above 2.2 and A260:A280 ratio above 1.8. mRNA was purified from total RNA with oligo(dT) beads and fractionized for cDNA synthesis, followed by first-strand cDNA amplification using random hexamer-primers. The second-strand cDNA was synthesized using DNA polymerase I, RNase H, dNTPs and buffer. Short double-stranded cDNA fragments were purified, added “A” base and ligated to Illumina sequencing adaptors. Next, DNA fragments sized in 150-200 bp were gel-purified and amplified by PCR. Small RNA library was constructed according to the Illumina TruSeq Small RNA Sample Preparation protocol. Briefly, RNA 3’ and 5’ adaptors were ligated to total RNA followed by reverse transcription. cDNA and small RNA libraries were sequenced on Illumina HiSeq 2500 platform in RiboBio Co. Ltd,. (Guang zhou, China). Illumina HiSeq 2500 gds 302322580 GEO Accession GSM2322580 SRX2178812 GSM2322581 SRP090167 SRS1704189 GSM2322581 miRNA-Seq TRANSCRIPTOMIC size fractionation Total RNA was isolated from cells using Trizol (Invitrogen, USA) according to manufacturer’s instructions. RNA cleanup involved DNase I digestion step was performed using RAeasy spin colmna (Qiagen), followed by assessment of RNA purity using the ND-1000 Nanodrop. Purified RNA samples had an A260:A230 ratio above 2.2 and A260:A280 ratio above 1.8. mRNA was purified from total RNA with oligo(dT) beads and fractionized for cDNA synthesis, followed by first-strand cDNA amplification using random hexamer-primers. The second-strand cDNA was synthesized using DNA polymerase I, RNase H, dNTPs and buffer. Short double-stranded cDNA fragments were purified, added “A” base and ligated to Illumina sequencing adaptors. Next, DNA fragments sized in 150-200 bp were gel-purified and amplified by PCR. Small RNA library was constructed according to the Illumina TruSeq Small RNA Sample Preparation protocol. Briefly, RNA 3’ and 5’ adaptors were ligated to total RNA followed by reverse transcription. cDNA and small RNA libraries were sequenced on Illumina HiSeq 2500 platform in RiboBio Co. Ltd,. (Guang zhou, China). Illumina HiSeq 2500 gds 302322581 GEO Accession GSM2322581