<STUDY_REF accession="SRP090176"> <IDENTIFIERS> <PRIMARY_ID>SRP090176</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA343381</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>DNA extraction and PCR amplification with bacterial genomic DNA from luminal fluid was extracted using the QIAamp DNA Stool mini Kit according to the manufacturerĄŻs instructions (Qiagen, German). DNA was quantified using a NanoDrop ND-2000 (NanoDrop Technologies, USA), For each DNA sample, 16S rRNA gene was amplified using fusion primer set specific for V3-V5 hypervariable regions(F:5-ĄäTCCTACGGGAGGCAGCAG-3Ąä and R:5Ąä-TGTGCGGGCCCCCGTCAATT-3Ąä ) contained adaptors, key sequence and barcode (Multiple IDentifier) sequences as described by the 454 Sequencing System Guidelines for Amplicon Experimental Design Roche,</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1704431"> <IDENTIFIERS> <PRIMARY_ID>SRS1704431</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN05786742</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>single data set</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <SINGLE/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <LS454> <INSTRUMENT_MODEL>454 GS FLX+</INSTRUMENT_MODEL> </LS454> </PLATFORM> </EXPERIMENT> </EXPERIMENT

SRX2179281 single data set <STUDY_REF accession="SRP090176"> <IDENTIFIERS> <PRIMARY_ID>SRP090176</PRIMARY_ID> <EXTERNAL_ID namespace="BioProject">PRJNA343381</EXTERNAL_ID> </IDENTIFIERS> </STUDY_REF> <DESIGN> <DESIGN_DESCRIPTION>DNA extraction and PCR amplification with bacterial genomic DNA from luminal fluid was extracted using the QIAamp DNA Stool mini Kit according to the manufacturerĄŻs instructions (Qiagen, German). DNA was quantified using a NanoDrop ND-2000 (NanoDrop Technologies, USA), For each DNA sample, 16S rRNA gene was amplified using fusion primer set specific for V3-V5 hypervariable regions(F:5-ĄäTCCTACGGGAGGCAGCAG-3Ąä and R:5Ąä-TGTGCGGGCCCCCGTCAATT-3Ąä ) contained adaptors, key sequence and barcode (Multiple IDentifier) sequences as described by the 454 Sequencing System Guidelines for Amplicon Experimental Design Roche,</DESIGN_DESCRIPTION> <SAMPLE_DESCRIPTOR accession="SRS1704431"> <IDENTIFIERS> <PRIMARY_ID>SRS1704431</PRIMARY_ID> <EXTERNAL_ID namespace="BioSample">SAMN05786742</EXTERNAL_ID> </IDENTIFIERS> </SAMPLE_DESCRIPTOR> <LIBRARY_DESCRIPTOR> <LIBRARY_NAME>single data set</LIBRARY_NAME> <LIBRARY_STRATEGY>AMPLICON</LIBRARY_STRATEGY> <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE> <LIBRARY_SELECTION>PCR</LIBRARY_SELECTION> <LIBRARY_LAYOUT> <SINGLE/> </LIBRARY_LAYOUT> </LIBRARY_DESCRIPTOR> </DESIGN> <PLATFORM> <LS454> <INSTRUMENT_MODEL>454 GS FLX+</INSTRUMENT_MODEL> </LS454> </PLATFORM> </EXPERIMENT> </EXPERIMENT_SET>