SRX2181461 SO_2627_Sample A SRP090201 PRJNA343588 Library preparation was performed at Genotypic Technology’s Genomics facility following Illumina TruSeq RNA library protocol outlined in “TruSeq RNA Sample Preparation Guide” (Part # 15008136; Rev. A; Nov 2010). 1ug of Total RNA was subjected to Poly A purification of mRNA. Purified mRNA was fragmented for 2 minutes at elevated temperature (94oC) in the presence of divalent cations and reverse transcribed with Superscript III Reverse transcriptase by priming with Random Hexamers. Second strand cDNA was synthesized in the presence of DNA Polymerase I and RnaseH. The cDNA was cleaned up using HighPrep PCR (Magbio). Illumina Adapters were ligated to the cDNA molecules after end repair and addition of A base. SPRI cleanup was performed after ligation. The library was amplified using 8 cycles of PCR for enrichment of adapter ligated fragments. The prepared library was quantified using Nanodrop and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent). SRS1705528 SAMN05787350 SO_2627_Sample A RNA-Seq TRANSCRIPTOMIC PCR NextSeq 500 SRX2181462 SO_2627_Sample B SRP090201 PRJNA343588 Library preparation was performed at Genotypic Technology’s Genomics facility following Illumina TruSeq RNA library protocol outlined in “TruSeq RNA Sample Preparation Guide” (Part # 15008136; Rev. A; Nov 2010). 1ug of Total RNA was subjected to Poly A purification of mRNA. Purified mRNA was fragmented for 2 minutes at elevated temperature (94oC) in the presence of divalent cations and reverse transcribed with Superscript III Reverse transcriptase by priming with Random Hexamers. Second strand cDNA was synthesized in the presence of DNA Polymerase I and RnaseH. The cDNA was cleaned up using HighPrep PCR (Magbio). Illumina Adapters were ligated to the cDNA molecules after end repair and addition of A base. SPRI cleanup was performed after ligation. The library was amplified using 8 cycles of PCR for enrichment of adapter ligated fragments. The prepared library was quantified using Nanodrop and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent). SRS1705529 SAMN05787351 SO_2627_Sample B RNA-Seq TRANSCRIPTOMIC PCR NextSeq 500 SRX2181463 SO_2627_Sample C SRP090201 PRJNA343588 Library preparation was performed at Genotypic Technology’s Genomics facility following Illumina TruSeq RNA library protocol outlined in “TruSeq RNA Sample Preparation Guide” (Part # 15008136; Rev. A; Nov 2010). 1ug of Total RNA was subjected to Poly A purification of mRNA. Purified mRNA was fragmented for 2 minutes at elevated temperature (94oC) in the presence of divalent cations and reverse transcribed with Superscript III Reverse transcriptase by priming with Random Hexamers. Second strand cDNA was synthesized in the presence of DNA Polymerase I and RnaseH. The cDNA was cleaned up using HighPrep PCR (Magbio). Illumina Adapters were ligated to the cDNA molecules after end repair and addition of A base. SPRI cleanup was performed after ligation. The library was amplified using 8 cycles of PCR for enrichment of adapter ligated fragments. The prepared library was quantified using Nanodrop and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent). SRS1705530 SAMN05791017 SO_2627_Sample C RNA-Seq TRANSCRIPTOMIC PCR NextSeq 500 SRX2181464 SO_2627_Sample F SRP090201 PRJNA343588 Library preparation was performed at Genotypic Technology’s Genomics facility following Illumina TruSeq RNA library protocol outlined in “TruSeq RNA Sample Preparation Guide” (Part # 15008136; Rev. A; Nov 2010). 1ug of Total RNA was subjected to Poly A purification of mRNA. Purified mRNA was fragmented for 2 minutes at elevated temperature (94oC) in the presence of divalent cations and reverse transcribed with Superscript III Reverse transcriptase by priming with Random Hexamers. Second strand cDNA was synthesized in the presence of DNA Polymerase I and RnaseH. The cDNA was cleaned up using HighPrep PCR (Magbio). Illumina Adapters were ligated to the cDNA molecules after end repair and addition of A base. SPRI cleanup was performed after ligation. The library was amplified using 8 cycles of PCR for enrichment of adapter ligated fragments. The prepared library was quantified using Nanodrop and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent). SRS1705532 SAMN05791019 SO_2627_Sample F RNA-Seq TRANSCRIPTOMIC PCR NextSeq 500 SRX2181465 SO_2627_Sample G SRP090201 PRJNA343588 Library preparation was performed at Genotypic Technology’s Genomics facility following Illumina TruSeq RNA library protocol outlined in “TruSeq RNA Sample Preparation Guide” (Part # 15008136; Rev. A; Nov 2010). 1ug of Total RNA was subjected to Poly A purification of mRNA. Purified mRNA was fragmented for 2 minutes at elevated temperature (94oC) in the presence of divalent cations and reverse transcribed with Superscript III Reverse transcriptase by priming with Random Hexamers. Second strand cDNA was synthesized in the presence of DNA Polymerase I and RnaseH. The cDNA was cleaned up using HighPrep PCR (Magbio). Illumina Adapters were ligated to the cDNA molecules after end repair and addition of A base. SPRI cleanup was performed after ligation. The library was amplified using 8 cycles of PCR for enrichment of adapter ligated fragments. The prepared library was quantified using Nanodrop and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent). SRS1705531 SAMN05791020 SO_2627_Sample G RNA-Seq TRANSCRIPTOMIC PCR NextSeq 500 SRX2181466 SO_2627_Sample H SRP090201 PRJNA343588 Library preparation was performed at Genotypic Technology’s Genomics facility following Illumina TruSeq RNA library protocol outlined in “TruSeq RNA Sample Preparation Guide” (Part # 15008136; Rev. A; Nov 2010). 1ug of Total RNA was subjected to Poly A purification of mRNA. Purified mRNA was fragmented for 2 minutes at elevated temperature (94oC) in the presence of divalent cations and reverse transcribed with Superscript III Reverse transcriptase by priming with Random Hexamers. Second strand cDNA was synthesized in the presence of DNA Polymerase I and RnaseH. The cDNA was cleaned up using HighPrep PCR (Magbio). Illumina Adapters were ligated to the cDNA molecules after end repair and addition of A base. SPRI cleanup was performed after ligation. The library was amplified using 8 cycles of PCR for enrichment of adapter ligated fragments. The prepared library was quantified using Nanodrop and validated for quality by running an aliquot on High Sensitivity Bioanalyzer Chip (Agilent). SRS1705533 SAMN05791021 SO_2627_Sample H RNA-Seq TRANSCRIPTOMIC PCR NextSeq 500