SRX2181537 Experiment of SCK SRP090209 PRJNA343700 A sunflower genotype, S18 (resistant to Sunflower Verticillium wilt), was used in this study.The sample SCK used as control plant was inoculated with 50 mL distilled water at the two-true-leaf growth stage.The RNAs were isolated from the sunflower sample's roots using Trizol reagent (Invitrogen, US)at 14 days age.The total RNA sample was first treated with DNase I to degrade any possible DNA contamination. Then the products was purified by magnetic beads. After that, the mRNA was enriched by using the oligo (dT) magnetic beads. It was mixed with the fragmentation buffer, the mRNAs were fragmented into short fragments (about 200 bp). Then the first strand of cDNA was synthesized by using random hexamer-primed reverse transcription. Buffer, dNTPs, RNase H and DNA polymerase I were added to synthesize the second strand. The double strand cDNA was purified with magnetic beads. End reparation was then performed. After the previous step, adaptors were ligated to the ends of these fragments. Next, ligation products were selected by size and purified on TAE-agarose gel. Finally, the fragments were enriched by PCR amplification, then purified by magnetic beads and dissolved in the appropriate amount of Epstein-Barr solution. During the QC step, Agilent 2100 Bioanaylzer were used to qualify and quantify of the sample library. The libraries were sequenced with an Ion Proton sequencer when necessary. SRS1705592 SAMN05792431 RNA-Seq TRANSCRIPTOMIC cDNA 0 0 Technical Read Adapter 1 1 Application Read Forward 5 Ion Torrent Proton