SRX2207949
Uhl_Replicate-1_AAGCG_CTAGG_R1.fq.HB.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725505
HB.2
Uhl_Replicate-1_AAGCG_CTAGG_R1.fq.HB.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207950
Uhl_Replicate-1_AAGCG_ttcTCAGC_R1.fq.M3C4-TQ_3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725506
M3C4-TQ_3
Uhl_Replicate-1_AAGCG_ttcTCAGC_R1.fq.M3C4-TQ_3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207951
Uhl_Replicate-1_atCGGTT_CTAGG_R1.fq.PH.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725508
PH.3
Uhl_Replicate-1_atCGGTT_CTAGG_R1.fq.PH.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207952
Uhl_Replicate-1_atCGGTT_ttcTCAGC_R1.fq.M3C4-TQ_1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725507
M3C4-TQ_1
Uhl_Replicate-1_atCGGTT_ttcTCAGC_R1.fq.M3C4-TQ_1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207953
Uhl_Replicate-1_cAACAC_ccACGTC_R1.fq.MOCK
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725509
MOCK
Uhl_Replicate-1_cAACAC_ccACGTC_R1.fq.MOCK
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207954
Uhl_Replicate-1_cAACAC_CTAGG_R1.fq.PH.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725510
PH.2
Uhl_Replicate-1_cAACAC_CTAGG_R1.fq.PH.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207955
Uhl_Replicate-1_ctGGATG_CTAGG_R1.fq.CA.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725511
CA.1
Uhl_Replicate-1_ctGGATG_CTAGG_R1.fq.CA.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207956
Uhl_Replicate-1_ctGGATG_ttcTCAGC_R1.fq.AP.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725512
AP.2
Uhl_Replicate-1_ctGGATG_ttcTCAGC_R1.fq.AP.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207957
Uhl_Replicate-1_gCCACA_CTAGG_R1.fq.HB.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725513
HB.3
Uhl_Replicate-1_gCCACA_CTAGG_R1.fq.HB.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207958
Uhl_Replicate-1_gCCACA_ttcTCAGC_R1.fq.AP.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725514
AP.1
Uhl_Replicate-1_gCCACA_ttcTCAGC_R1.fq.AP.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207959
Uhl_Replicate-1_GGTAC_CTAGG_R1.fq.PH.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725515
PH.1
Uhl_Replicate-1_GGTAC_CTAGG_R1.fq.PH.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207960
Uhl_Replicate-1_GGTAC_tGCTTA_R1.fq.CA.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725516
CA.3
Uhl_Replicate-1_GGTAC_tGCTTA_R1.fq.CA.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207961
Uhl_Replicate-1_tcgGTCAA_CTAGG_R1.fq.HB.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725517
HB.1
Uhl_Replicate-1_tcgGTCAA_CTAGG_R1.fq.HB.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207962
Uhl_Replicate-1_tcgGTCAA_ttcTCAGC_R1.fq.M3C4-TQ_2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725518
M3C4-TQ_2
Uhl_Replicate-1_tcgGTCAA_ttcTCAGC_R1.fq.M3C4-TQ_2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207963
Uhl_Replicate-1_tgaTTGAC_CTAGG_R1.fq.CA.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725519
CA.2
Uhl_Replicate-1_tgaTTGAC_CTAGG_R1.fq.CA.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207964
Uhl_Replicate-1_tgaTTGAC_ttcTCAGC_R1.fq.AP.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725520
AP.3
Uhl_Replicate-1_tgaTTGAC_ttcTCAGC_R1.fq.AP.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207965
Uhl_Replicate-2_AAGCG_CTAGG_R1.fq.HB.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725505
HB.2
Uhl_Replicate-2_AAGCG_CTAGG_R1.fq.HB.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207966
Uhl_Replicate-2_AAGCG_ttcTCAGC_R1.fq.M3C4-TQ_3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725506
M3C4-TQ_3
Uhl_Replicate-2_AAGCG_ttcTCAGC_R1.fq.M3C4-TQ_3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207967
Uhl_Replicate-2_atCGGTT_CTAGG_R1.fq.PH.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725508
PH.3
Uhl_Replicate-2_atCGGTT_CTAGG_R1.fq.PH.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207968
Uhl_Replicate-2_atCGGTT_ttcTCAGC_R1.fq.M3C4-TQ_1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725507
M3C4-TQ_1
Uhl_Replicate-2_atCGGTT_ttcTCAGC_R1.fq.M3C4-TQ_1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207969
Uhl_Replicate-2_cAACAC_ccACGTC_R1.fq.MOCK
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725509
MOCK
Uhl_Replicate-2_cAACAC_ccACGTC_R1.fq.MOCK
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207970
Uhl_Replicate-2_cAACAC_CTAGG_R1.fq.PH.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725510
PH.2
Uhl_Replicate-2_cAACAC_CTAGG_R1.fq.PH.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207971
Uhl_Replicate-2_ctGGATG_CTAGG_R1.fq.CA.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725511
CA.1
Uhl_Replicate-2_ctGGATG_CTAGG_R1.fq.CA.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207972
Uhl_Replicate-2_ctGGATG_ttcTCAGC_R1.fq.AP.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725512
AP.2
Uhl_Replicate-2_ctGGATG_ttcTCAGC_R1.fq.AP.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207973
Uhl_Replicate-2_gCCACA_CTAGG_R1.fq.HB.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725513
HB.3
Uhl_Replicate-2_gCCACA_CTAGG_R1.fq.HB.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207974
Uhl_Replicate-2_gCCACA_ttcTCAGC_R1.fq.AP.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725514
AP.1
Uhl_Replicate-2_gCCACA_ttcTCAGC_R1.fq.AP.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207975
Uhl_Replicate-2_GGTAC_CTAGG_R1.fq.PH.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725515
PH.1
Uhl_Replicate-2_GGTAC_CTAGG_R1.fq.PH.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207976
Uhl_Replicate-2_GGTAC_tGCTTA_R1.fq.CA.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725516
CA.3
Uhl_Replicate-2_GGTAC_tGCTTA_R1.fq.CA.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207977
Uhl_Replicate-2_tcgGTCAA_CTAGG_R1.fq.HB.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725517
HB.1
Uhl_Replicate-2_tcgGTCAA_CTAGG_R1.fq.HB.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207978
Uhl_Replicate-2_tcgGTCAA_ttcTCAGC_R1.fq.M3C4-TQ_2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725518
M3C4-TQ_2
Uhl_Replicate-2_tcgGTCAA_ttcTCAGC_R1.fq.M3C4-TQ_2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207979
Uhl_Replicate-2_tgaTTGAC_CTAGG_R1.fq.CA.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725519
CA.2
Uhl_Replicate-2_tgaTTGAC_CTAGG_R1.fq.CA.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207980
Uhl_Replicate-2_tgaTTGAC_ttcTCAGC_R1.fq.AP.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725520
AP.3
Uhl_Replicate-2_tgaTTGAC_ttcTCAGC_R1.fq.AP.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207981
Uhl_Replicate-3_AAGCG_CTAGG_R1.fq.HB.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725505
HB.2
Uhl_Replicate-3_AAGCG_CTAGG_R1.fq.HB.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207982
Uhl_Replicate-3_AAGCG_ttcTCAGC_R1.fq.M3C4-TQ_3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725506
M3C4-TQ_3
Uhl_Replicate-3_AAGCG_ttcTCAGC_R1.fq.M3C4-TQ_3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207983
Uhl_Replicate-3_atCGGTT_CTAGG_R1.fq.PH.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725508
PH.3
Uhl_Replicate-3_atCGGTT_CTAGG_R1.fq.PH.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207984
Uhl_Replicate-3_atCGGTT_ttcTCAGC_R1.fq.M3C4-TQ_1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725507
M3C4-TQ_1
Uhl_Replicate-3_atCGGTT_ttcTCAGC_R1.fq.M3C4-TQ_1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207985
Uhl_Replicate-3_cAACAC_ccACGTC_R1.fq.MOCK
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725509
MOCK
Uhl_Replicate-3_cAACAC_ccACGTC_R1.fq.MOCK
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207986
Uhl_Replicate-3_cAACAC_CTAGG_R1.fq.PH.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725510
PH.2
Uhl_Replicate-3_cAACAC_CTAGG_R1.fq.PH.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207987
Uhl_Replicate-3_ctGGATG_CTAGG_R1.fq.CA.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725511
CA.1
Uhl_Replicate-3_ctGGATG_CTAGG_R1.fq.CA.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207988
Uhl_Replicate-3_ctGGATG_ttcTCAGC_R1.fq.AP.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725512
AP.2
Uhl_Replicate-3_ctGGATG_ttcTCAGC_R1.fq.AP.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207989
Uhl_Replicate-3_gCCACA_CTAGG_R1.fq.HB.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725513
HB.3
Uhl_Replicate-3_gCCACA_CTAGG_R1.fq.HB.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207990
Uhl_Replicate-3_gCCACA_ttcTCAGC_R1.fq.AP.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725514
AP.1
Uhl_Replicate-3_gCCACA_ttcTCAGC_R1.fq.AP.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207991
Uhl_Replicate-3_GGTAC_CTAGG_R1.fq.PH.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725515
PH.1
Uhl_Replicate-3_GGTAC_CTAGG_R1.fq.PH.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207992
Uhl_Replicate-3_GGTAC_tGCTTA_R1.fq.CA.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725516
CA.3
Uhl_Replicate-3_GGTAC_tGCTTA_R1.fq.CA.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207993
Uhl_Replicate-3_tcgGTCAA_CTAGG_R1.fq.HB.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725517
HB.1
Uhl_Replicate-3_tcgGTCAA_CTAGG_R1.fq.HB.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207994
Uhl_Replicate-3_tcgGTCAA_ttcTCAGC_R1.fq.M3C4-TQ_2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725518
M3C4-TQ_2
Uhl_Replicate-3_tcgGTCAA_ttcTCAGC_R1.fq.M3C4-TQ_2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207995
Uhl_Replicate-3_tgaTTGAC_CTAGG_R1.fq.CA.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725519
CA.2
Uhl_Replicate-3_tgaTTGAC_CTAGG_R1.fq.CA.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207996
Uhl_Replicate-3_tgaTTGAC_ttcTCAGC_R1.fq.AP.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725520
AP.3
Uhl_Replicate-3_tgaTTGAC_ttcTCAGC_R1.fq.AP.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207997
Uhl_Replicate-4_AAGCG_CTAGG_R1.fq.HB.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725505
HB.2
Uhl_Replicate-4_AAGCG_CTAGG_R1.fq.HB.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207998
Uhl_Replicate-4_AAGCG_ttcTCAGC_R1.fq.M3C4-TQ_3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725506
M3C4-TQ_3
Uhl_Replicate-4_AAGCG_ttcTCAGC_R1.fq.M3C4-TQ_3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2207999
Uhl_Replicate-4_atCGGTT_CTAGG_R1.fq.PH.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725508
PH.3
Uhl_Replicate-4_atCGGTT_CTAGG_R1.fq.PH.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208000
Uhl_Replicate-4_atCGGTT_ttcTCAGC_R1.fq.M3C4-TQ_1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725507
M3C4-TQ_1
Uhl_Replicate-4_atCGGTT_ttcTCAGC_R1.fq.M3C4-TQ_1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208001
Uhl_Replicate-4_cAACAC_ccACGTC_R1.fq.MOCK
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725509
MOCK
Uhl_Replicate-4_cAACAC_ccACGTC_R1.fq.MOCK
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208002
Uhl_Replicate-4_cAACAC_CTAGG_R1.fq.PH.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725510
PH.2
Uhl_Replicate-4_cAACAC_CTAGG_R1.fq.PH.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208003
Uhl_Replicate-4_ctGGATG_CTAGG_R1.fq.CA.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725511
CA.1
Uhl_Replicate-4_ctGGATG_CTAGG_R1.fq.CA.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208004
Uhl_Replicate-4_ctGGATG_ttcTCAGC_R1.fq.AP.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725512
AP.2
Uhl_Replicate-4_ctGGATG_ttcTCAGC_R1.fq.AP.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208005
Uhl_Replicate-4_gCCACA_CTAGG_R1.fq.HB.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725513
HB.3
Uhl_Replicate-4_gCCACA_CTAGG_R1.fq.HB.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208006
Uhl_Replicate-4_gCCACA_ttcTCAGC_R1.fq.AP.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725514
AP.1
Uhl_Replicate-4_gCCACA_ttcTCAGC_R1.fq.AP.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208007
Uhl_Replicate-4_GGTAC_CTAGG_R1.fq.PH.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725515
PH.1
Uhl_Replicate-4_GGTAC_CTAGG_R1.fq.PH.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208008
Uhl_Replicate-4_GGTAC_tGCTTA_R1.fq.CA.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725516
CA.3
Uhl_Replicate-4_GGTAC_tGCTTA_R1.fq.CA.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208009
Uhl_Replicate-4_tcgGTCAA_CTAGG_R1.fq.HB.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725517
HB.1
Uhl_Replicate-4_tcgGTCAA_CTAGG_R1.fq.HB.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208010
Uhl_Replicate-4_tcgGTCAA_ttcTCAGC_R1.fq.M3C4-TQ_2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725518
M3C4-TQ_2
Uhl_Replicate-4_tcgGTCAA_ttcTCAGC_R1.fq.M3C4-TQ_2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208011
Uhl_Replicate-4_tgaTTGAC_CTAGG_R1.fq.CA.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725519
CA.2
Uhl_Replicate-4_tgaTTGAC_CTAGG_R1.fq.CA.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208012
Uhl_Replicate-4_tgaTTGAC_ttcTCAGC_R1.fq.AP.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725520
AP.3
Uhl_Replicate-4_tgaTTGAC_ttcTCAGC_R1.fq.AP.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208013
Uhl_Replicate-5_AAGCG_CTAGG_R1.fq.HB.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725505
HB.2
Uhl_Replicate-5_AAGCG_CTAGG_R1.fq.HB.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208014
Uhl_Replicate-5_AAGCG_ttcTCAGC_R1.fq.M3C4-TQ_3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725506
M3C4-TQ_3
Uhl_Replicate-5_AAGCG_ttcTCAGC_R1.fq.M3C4-TQ_3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208015
Uhl_Replicate-5_atCGGTT_CTAGG_R1.fq.PH.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725508
PH.3
Uhl_Replicate-5_atCGGTT_CTAGG_R1.fq.PH.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208016
Uhl_Replicate-5_atCGGTT_ttcTCAGC_R1.fq.M3C4-TQ_1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725507
M3C4-TQ_1
Uhl_Replicate-5_atCGGTT_ttcTCAGC_R1.fq.M3C4-TQ_1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208017
Uhl_Replicate-5_cAACAC_ccACGTC_R1.fq.MOCK
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725509
MOCK
Uhl_Replicate-5_cAACAC_ccACGTC_R1.fq.MOCK
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208018
Uhl_Replicate-5_cAACAC_CTAGG_R1.fq.PH.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725510
PH.2
Uhl_Replicate-5_cAACAC_CTAGG_R1.fq.PH.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208019
Uhl_Replicate-5_ctGGATG_CTAGG_R1.fq.CA.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725511
CA.1
Uhl_Replicate-5_ctGGATG_CTAGG_R1.fq.CA.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208020
Uhl_Replicate-5_ctGGATG_ttcTCAGC_R1.fq.AP.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725512
AP.2
Uhl_Replicate-5_ctGGATG_ttcTCAGC_R1.fq.AP.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208021
Uhl_Replicate-5_gCCACA_CTAGG_R1.fq.HB.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725513
HB.3
Uhl_Replicate-5_gCCACA_CTAGG_R1.fq.HB.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208022
Uhl_Replicate-5_gCCACA_ttcTCAGC_R1.fq.AP.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725514
AP.1
Uhl_Replicate-5_gCCACA_ttcTCAGC_R1.fq.AP.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208023
Uhl_Replicate-5_GGTAC_CTAGG_R1.fq.PH.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725515
PH.1
Uhl_Replicate-5_GGTAC_CTAGG_R1.fq.PH.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208024
Uhl_Replicate-5_GGTAC_tGCTTA_R1.fq.CA.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725516
CA.3
Uhl_Replicate-5_GGTAC_tGCTTA_R1.fq.CA.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208025
Uhl_Replicate-5_tcgGTCAA_CTAGG_R1.fq.HB.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725517
HB.1
Uhl_Replicate-5_tcgGTCAA_CTAGG_R1.fq.HB.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208026
Uhl_Replicate-5_tcgGTCAA_ttcTCAGC_R1.fq.M3C4-TQ_2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725518
M3C4-TQ_2
Uhl_Replicate-5_tcgGTCAA_ttcTCAGC_R1.fq.M3C4-TQ_2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208027
Uhl_Replicate-5_tgaTTGAC_CTAGG_R1.fq.CA.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725519
CA.2
Uhl_Replicate-5_tgaTTGAC_CTAGG_R1.fq.CA.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208028
Uhl_Replicate-5_tgaTTGAC_ttcTCAGC_R1.fq.AP.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725520
AP.3
Uhl_Replicate-5_tgaTTGAC_ttcTCAGC_R1.fq.AP.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208029
Uhl_Replicate-6_AAGCG_CTAGG_R1.fq.HB.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725505
HB.2
Uhl_Replicate-6_AAGCG_CTAGG_R1.fq.HB.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208030
Uhl_Replicate-6_AAGCG_ttcTCAGC_R1.fq.M3C4-TQ_3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725506
M3C4-TQ_3
Uhl_Replicate-6_AAGCG_ttcTCAGC_R1.fq.M3C4-TQ_3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208031
Uhl_Replicate-6_atCGGTT_CTAGG_R1.fq.PH.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725508
PH.3
Uhl_Replicate-6_atCGGTT_CTAGG_R1.fq.PH.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208032
Uhl_Replicate-6_atCGGTT_ttcTCAGC_R1.fq.M3C4-TQ_1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725507
M3C4-TQ_1
Uhl_Replicate-6_atCGGTT_ttcTCAGC_R1.fq.M3C4-TQ_1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208033
Uhl_Replicate-6_cAACAC_ccACGTC_R1.fq.MOCK
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725509
MOCK
Uhl_Replicate-6_cAACAC_ccACGTC_R1.fq.MOCK
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208034
Uhl_Replicate-6_cAACAC_CTAGG_R1.fq.PH.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725510
PH.2
Uhl_Replicate-6_cAACAC_CTAGG_R1.fq.PH.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208035
Uhl_Replicate-6_ctGGATG_CTAGG_R1.fq.CA.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725511
CA.1
Uhl_Replicate-6_ctGGATG_CTAGG_R1.fq.CA.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208036
Uhl_Replicate-6_ctGGATG_ttcTCAGC_R1.fq.AP.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725512
AP.2
Uhl_Replicate-6_ctGGATG_ttcTCAGC_R1.fq.AP.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208037
Uhl_Replicate-6_gCCACA_CTAGG_R1.fq.HB.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725513
HB.3
Uhl_Replicate-6_gCCACA_CTAGG_R1.fq.HB.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208038
Uhl_Replicate-6_gCCACA_ttcTCAGC_R1.fq.AP.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725514
AP.1
Uhl_Replicate-6_gCCACA_ttcTCAGC_R1.fq.AP.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208039
Uhl_Replicate-6_GGTAC_CTAGG_R1.fq.PH.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725515
PH.1
Uhl_Replicate-6_GGTAC_CTAGG_R1.fq.PH.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208040
Uhl_Replicate-6_GGTAC_tGCTTA_R1.fq.CA.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725516
CA.3
Uhl_Replicate-6_GGTAC_tGCTTA_R1.fq.CA.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208041
Uhl_Replicate-6_tcgGTCAA_CTAGG_R1.fq.HB.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725517
HB.1
Uhl_Replicate-6_tcgGTCAA_CTAGG_R1.fq.HB.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208042
Uhl_Replicate-6_tcgGTCAA_ttcTCAGC_R1.fq.M3C4-TQ_2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725518
M3C4-TQ_2
Uhl_Replicate-6_tcgGTCAA_ttcTCAGC_R1.fq.M3C4-TQ_2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208043
Uhl_Replicate-6_tgaTTGAC_CTAGG_R1.fq.CA.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725519
CA.2
Uhl_Replicate-6_tgaTTGAC_CTAGG_R1.fq.CA.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208044
Uhl_Replicate-6_tgaTTGAC_ttcTCAGC_R1.fq.AP.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725520
AP.3
Uhl_Replicate-6_tgaTTGAC_ttcTCAGC_R1.fq.AP.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208045
Uhl_Replicate-7_AAGCG_CTAGG_R1.fq.HB.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725505
HB.2
Uhl_Replicate-7_AAGCG_CTAGG_R1.fq.HB.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208046
Uhl_Replicate-7_AAGCG_ttcTCAGC_R1.fq.M3C4-TQ_3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725506
M3C4-TQ_3
Uhl_Replicate-7_AAGCG_ttcTCAGC_R1.fq.M3C4-TQ_3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208047
Uhl_Replicate-7_atCGGTT_CTAGG_R1.fq.PH.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725508
PH.3
Uhl_Replicate-7_atCGGTT_CTAGG_R1.fq.PH.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208048
Uhl_Replicate-7_atCGGTT_ttcTCAGC_R1.fq.M3C4-TQ_1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725507
M3C4-TQ_1
Uhl_Replicate-7_atCGGTT_ttcTCAGC_R1.fq.M3C4-TQ_1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208049
Uhl_Replicate-7_cAACAC_ccACGTC_R1.fq.MOCK
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725509
MOCK
Uhl_Replicate-7_cAACAC_ccACGTC_R1.fq.MOCK
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208050
Uhl_Replicate-7_cAACAC_CTAGG_R1.fq.PH.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725510
PH.2
Uhl_Replicate-7_cAACAC_CTAGG_R1.fq.PH.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208051
Uhl_Replicate-7_ctGGATG_CTAGG_R1.fq.CA.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725511
CA.1
Uhl_Replicate-7_ctGGATG_CTAGG_R1.fq.CA.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208052
Uhl_Replicate-7_ctGGATG_ttcTCAGC_R1.fq.AP.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725512
AP.2
Uhl_Replicate-7_ctGGATG_ttcTCAGC_R1.fq.AP.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208053
Uhl_Replicate-7_gCCACA_CTAGG_R1.fq.HB.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725513
HB.3
Uhl_Replicate-7_gCCACA_CTAGG_R1.fq.HB.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208054
Uhl_Replicate-7_gCCACA_ttcTCAGC_R1.fq.AP.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725514
AP.1
Uhl_Replicate-7_gCCACA_ttcTCAGC_R1.fq.AP.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208055
Uhl_Replicate-7_GGTAC_CTAGG_R1.fq.PH.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725515
PH.1
Uhl_Replicate-7_GGTAC_CTAGG_R1.fq.PH.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208056
Uhl_Replicate-7_GGTAC_tGCTTA_R1.fq.CA.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725516
CA.3
Uhl_Replicate-7_GGTAC_tGCTTA_R1.fq.CA.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208057
Uhl_Replicate-7_tcgGTCAA_CTAGG_R1.fq.HB.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725517
HB.1
Uhl_Replicate-7_tcgGTCAA_CTAGG_R1.fq.HB.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208058
Uhl_Replicate-7_tcgGTCAA_ttcTCAGC_R1.fq.M3C4-TQ_2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725518
M3C4-TQ_2
Uhl_Replicate-7_tcgGTCAA_ttcTCAGC_R1.fq.M3C4-TQ_2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208059
Uhl_Replicate-7_tgaTTGAC_CTAGG_R1.fq.CA.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725519
CA.2
Uhl_Replicate-7_tgaTTGAC_CTAGG_R1.fq.CA.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208060
Uhl_Replicate-7_tgaTTGAC_ttcTCAGC_R1.fq.AP.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725520
AP.3
Uhl_Replicate-7_tgaTTGAC_ttcTCAGC_R1.fq.AP.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208061
Uhl_Replicate-8_AAGCG_CTAGG_R1.fq.HB.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725505
HB.2
Uhl_Replicate-8_AAGCG_CTAGG_R1.fq.HB.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208062
Uhl_Replicate-8_AAGCG_ttcTCAGC_R1.fq.M3C4-TQ_3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725506
M3C4-TQ_3
Uhl_Replicate-8_AAGCG_ttcTCAGC_R1.fq.M3C4-TQ_3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208063
Uhl_Replicate-8_atCGGTT_CTAGG_R1.fq.PH.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725508
PH.3
Uhl_Replicate-8_atCGGTT_CTAGG_R1.fq.PH.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208064
Uhl_Replicate-8_atCGGTT_ttcTCAGC_R1.fq.M3C4-TQ_1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725507
M3C4-TQ_1
Uhl_Replicate-8_atCGGTT_ttcTCAGC_R1.fq.M3C4-TQ_1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208065
Uhl_Replicate-8_cAACAC_ccACGTC_R1.fq.MOCK
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725509
MOCK
Uhl_Replicate-8_cAACAC_ccACGTC_R1.fq.MOCK
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208066
Uhl_Replicate-8_cAACAC_CTAGG_R1.fq.PH.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725510
PH.2
Uhl_Replicate-8_cAACAC_CTAGG_R1.fq.PH.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208067
Uhl_Replicate-8_ctGGATG_CTAGG_R1.fq.CA.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725511
CA.1
Uhl_Replicate-8_ctGGATG_CTAGG_R1.fq.CA.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208068
Uhl_Replicate-8_ctGGATG_ttcTCAGC_R1.fq.AP.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725512
AP.2
Uhl_Replicate-8_ctGGATG_ttcTCAGC_R1.fq.AP.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208069
Uhl_Replicate-8_gCCACA_CTAGG_R1.fq.HB.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725513
HB.3
Uhl_Replicate-8_gCCACA_CTAGG_R1.fq.HB.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208070
Uhl_Replicate-8_gCCACA_ttcTCAGC_R1.fq.AP.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725514
AP.1
Uhl_Replicate-8_gCCACA_ttcTCAGC_R1.fq.AP.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208071
Uhl_Replicate-8_GGTAC_CTAGG_R1.fq.PH.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725515
PH.1
Uhl_Replicate-8_GGTAC_CTAGG_R1.fq.PH.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208072
Uhl_Replicate-8_GGTAC_tGCTTA_R1.fq.CA.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725516
CA.3
Uhl_Replicate-8_GGTAC_tGCTTA_R1.fq.CA.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208073
Uhl_Replicate-8_tcgGTCAA_CTAGG_R1.fq.HB.1
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725517
HB.1
Uhl_Replicate-8_tcgGTCAA_CTAGG_R1.fq.HB.1
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208074
Uhl_Replicate-8_tcgGTCAA_ttcTCAGC_R1.fq.M3C4-TQ_2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725518
M3C4-TQ_2
Uhl_Replicate-8_tcgGTCAA_ttcTCAGC_R1.fq.M3C4-TQ_2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208075
Uhl_Replicate-8_tgaTTGAC_CTAGG_R1.fq.CA.2
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725519
CA.2
Uhl_Replicate-8_tgaTTGAC_CTAGG_R1.fq.CA.2
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq
SRX2208076
Uhl_Replicate-8_tgaTTGAC_ttcTCAGC_R1.fq.AP.3
SRP090758
Plant metabolites can trigger biodegradation of environmental pollutants.
SRS1725520
AP.3
Uhl_Replicate-8_tgaTTGAC_ttcTCAGC_R1.fq.AP.3
AMPLICON
METAGENOMIC
PCR
"The DNA extraction was performed with the FastDNA Spin Kit for Soil (MP Bio, USA) according to manufacturer’s instructions. Primers 515 forward 5’-GTGYCAGCMGCNGCGG-3’ and 926 reverse 5’-CCGYCAATTYMTTTRAGTTT-3’ (adapted from Walters et al. (2016) while modifying the forward primer) were utilized to target hypervariable regions V4–V5 of the 16S rRNA genes amplified by PCR in a final volume of 15 µL with: KAPA HiFi HotStart ReadyMix (Kapa Biosystems, Boston, MA, USA) containing 0.02 U/µL of KAPA HiFi HotStart DNA Polymerase, 2.5 mM MgCl2 and 0.3 mM of each dNTP; 0.3 µM of each primer (Generi Biotech, Czech Republic); template DNA (~20 ng). The cycling program was set as follows: 5 min at 95 °C, 20 cycles of 20 s at 98 °C, 15 s at 56 °C, 15 s at 72 °C and a final extension of 5 min at 72 °C. A volume of 0.5 µL of the PCR product was utilized as template for another round of PCR, performed under the same conditions, except that the final reaction volume was 25 µL, with 1 µM of each primer, and the cycle number was decreased to 5-10. The forward and reverse primers utilized for the second PCR were modified with sequencing adapters for Illumina sequencing systems . The resultant PCR products were purified using AMPure XP Beads (Agencourt, Beckman Coulter, USA) and were further indexed and sequenced on a MiSeq sequencing system at the University of Alaska Fairbanks core facility."
Illumina MiSeq