SRX2409398 Peds015_p15 SRP078093 phs001121 To construct the RNA sequencing library, we isolated RNA from tissue or cell line samples and selected for poly(A)-enriched RNA. The RNA was fragmented, treated with DNase, and dephosphorylated. These RNA fragments were purified and the sample underwent RNA hydrolysis, ssDNA adapter ligation to the 3' end, followed by PCR enrichment. We then size selected the RNA molecules and sequenced them on a HiSeq 2500. SRS1546381 CLF-PED-015-T Peds015_p15 RNA-Seq TRANSCRIPTOMIC other 204 0 Application Read Forward 1 1 Application Read Reverse 103 Illumina HiSeq 2500 alignment_software TopHat v1.4.1