SRP095345 PRJNA358055 GSE92565 High depth sequencing of BrU-labeled nascent RNA. BrU incorporation into nascent RNA followed by immunoprecipitation with a BrU specific antibody. The nascent RNA was subsequently eluted with BrU competition. Overall design: HEK293T cells were incubated with 2mM bromouridine for 15min labeling time and chased with 20mM uridine for different time intervals (0min, 15min, 30min and 60min). Nascent RNA was purified during BrU-IP with anti-BrdU. GSE92565 pubmed 28250203