SRX2440126 GSM2436965 SRP095530 SRS1874713 GSM2436965 RNA-Seq TRANSCRIPTOMIC cDNA After collecting 60-80 dissected VNCs on dry ice, the tissue wash crushed in 500ul of Trizol with glass beads and a pestle. This preparation was then stored at -80 degrees until all the samples were ready for RNA extraction. RNA extraction was performed by first spinning the crushed material at 11,000G for 10 minutes at 4 degrees C to pellet cuticle and lipids. Trizol supernatant was pulled off the pellet and 1/5 volume of chloroform was added and mixed in a 5’ Prime- heavy phase lock gel and centrifuged. The aqueous phase was pulled off and the RNA was cleaned and isolated on a Zymo Research RCC5 column. An on column DNase digestion was performed to degrade genomic DNA. The RNA was quantified using the Qubit fluorometer before sending total RNA samples to the Massively Parallel Sequencing Shared Resource (MPSSR) at Oregon Health and Science University (OHSU) for library preparation. Briefly, total RNA concentration and sample integrity was assessed via running on an Agilent RNA 6000 Pico chip on an Agilent Technologies 2100 Bioanalyzer instrument. Following quantification and quality control, 325ng of total RNA was subjected to ribosomal RNA reduction via Epicenter’s Ribo-Zero™ Gold kit (Human/Mouse/Rat). The output of this was taken into Illumina’s TruSeq® RNA Sample Preparation v2, beginning with the RNA fragmentation step. It should be noted that a poly(A) selection was not performed due to limited starting total RNA. Barcode indexing adapters were ligated and all 10 samples (5 control and 5 injured) were sequenced (100 bp single reads) on a single flow cell lane on the Illumina HiSeq 2500 Seqeuncer Illumina HiSeq 2500 gds 302436965 GEO Accession GSM2436965 SRX2440127 GSM2436966 SRP095530 SRS1874714 GSM2436966 RNA-Seq TRANSCRIPTOMIC cDNA After collecting 60-80 dissected VNCs on dry ice, the tissue wash crushed in 500ul of Trizol with glass beads and a pestle. This preparation was then stored at -80 degrees until all the samples were ready for RNA extraction. RNA extraction was performed by first spinning the crushed material at 11,000G for 10 minutes at 4 degrees C to pellet cuticle and lipids. Trizol supernatant was pulled off the pellet and 1/5 volume of chloroform was added and mixed in a 5’ Prime- heavy phase lock gel and centrifuged. The aqueous phase was pulled off and the RNA was cleaned and isolated on a Zymo Research RCC5 column. An on column DNase digestion was performed to degrade genomic DNA. The RNA was quantified using the Qubit fluorometer before sending total RNA samples to the Massively Parallel Sequencing Shared Resource (MPSSR) at Oregon Health and Science University (OHSU) for library preparation. Briefly, total RNA concentration and sample integrity was assessed via running on an Agilent RNA 6000 Pico chip on an Agilent Technologies 2100 Bioanalyzer instrument. Following quantification and quality control, 325ng of total RNA was subjected to ribosomal RNA reduction via Epicenter’s Ribo-Zero™ Gold kit (Human/Mouse/Rat). The output of this was taken into Illumina’s TruSeq® RNA Sample Preparation v2, beginning with the RNA fragmentation step. It should be noted that a poly(A) selection was not performed due to limited starting total RNA. Barcode indexing adapters were ligated and all 10 samples (5 control and 5 injured) were sequenced (100 bp single reads) on a single flow cell lane on the Illumina HiSeq 2500 Seqeuncer Illumina HiSeq 2500 gds 302436966 GEO Accession GSM2436966 SRX2440128 GSM2436967 SRP095530 SRS1874716 GSM2436967 RNA-Seq TRANSCRIPTOMIC cDNA After collecting 60-80 dissected VNCs on dry ice, the tissue wash crushed in 500ul of Trizol with glass beads and a pestle. This preparation was then stored at -80 degrees until all the samples were ready for RNA extraction. RNA extraction was performed by first spinning the crushed material at 11,000G for 10 minutes at 4 degrees C to pellet cuticle and lipids. Trizol supernatant was pulled off the pellet and 1/5 volume of chloroform was added and mixed in a 5’ Prime- heavy phase lock gel and centrifuged. The aqueous phase was pulled off and the RNA was cleaned and isolated on a Zymo Research RCC5 column. An on column DNase digestion was performed to degrade genomic DNA. The RNA was quantified using the Qubit fluorometer before sending total RNA samples to the Massively Parallel Sequencing Shared Resource (MPSSR) at Oregon Health and Science University (OHSU) for library preparation. Briefly, total RNA concentration and sample integrity was assessed via running on an Agilent RNA 6000 Pico chip on an Agilent Technologies 2100 Bioanalyzer instrument. Following quantification and quality control, 325ng of total RNA was subjected to ribosomal RNA reduction via Epicenter’s Ribo-Zero™ Gold kit (Human/Mouse/Rat). The output of this was taken into Illumina’s TruSeq® RNA Sample Preparation v2, beginning with the RNA fragmentation step. It should be noted that a poly(A) selection was not performed due to limited starting total RNA. Barcode indexing adapters were ligated and all 10 samples (5 control and 5 injured) were sequenced (100 bp single reads) on a single flow cell lane on the Illumina HiSeq 2500 Seqeuncer Illumina HiSeq 2500 gds 302436967 GEO Accession GSM2436967 SRX2440129 GSM2436968 SRP095530 SRS1874715 GSM2436968 RNA-Seq TRANSCRIPTOMIC cDNA After collecting 60-80 dissected VNCs on dry ice, the tissue wash crushed in 500ul of Trizol with glass beads and a pestle. This preparation was then stored at -80 degrees until all the samples were ready for RNA extraction. RNA extraction was performed by first spinning the crushed material at 11,000G for 10 minutes at 4 degrees C to pellet cuticle and lipids. Trizol supernatant was pulled off the pellet and 1/5 volume of chloroform was added and mixed in a 5’ Prime- heavy phase lock gel and centrifuged. The aqueous phase was pulled off and the RNA was cleaned and isolated on a Zymo Research RCC5 column. An on column DNase digestion was performed to degrade genomic DNA. The RNA was quantified using the Qubit fluorometer before sending total RNA samples to the Massively Parallel Sequencing Shared Resource (MPSSR) at Oregon Health and Science University (OHSU) for library preparation. Briefly, total RNA concentration and sample integrity was assessed via running on an Agilent RNA 6000 Pico chip on an Agilent Technologies 2100 Bioanalyzer instrument. Following quantification and quality control, 325ng of total RNA was subjected to ribosomal RNA reduction via Epicenter’s Ribo-Zero™ Gold kit (Human/Mouse/Rat). The output of this was taken into Illumina’s TruSeq® RNA Sample Preparation v2, beginning with the RNA fragmentation step. It should be noted that a poly(A) selection was not performed due to limited starting total RNA. Barcode indexing adapters were ligated and all 10 samples (5 control and 5 injured) were sequenced (100 bp single reads) on a single flow cell lane on the Illumina HiSeq 2500 Seqeuncer Illumina HiSeq 2500 gds 302436968 GEO Accession GSM2436968 SRX2440130 GSM2436969 SRP095530 SRS1874717 GSM2436969 RNA-Seq TRANSCRIPTOMIC cDNA After collecting 60-80 dissected VNCs on dry ice, the tissue wash crushed in 500ul of Trizol with glass beads and a pestle. This preparation was then stored at -80 degrees until all the samples were ready for RNA extraction. RNA extraction was performed by first spinning the crushed material at 11,000G for 10 minutes at 4 degrees C to pellet cuticle and lipids. Trizol supernatant was pulled off the pellet and 1/5 volume of chloroform was added and mixed in a 5’ Prime- heavy phase lock gel and centrifuged. The aqueous phase was pulled off and the RNA was cleaned and isolated on a Zymo Research RCC5 column. An on column DNase digestion was performed to degrade genomic DNA. The RNA was quantified using the Qubit fluorometer before sending total RNA samples to the Massively Parallel Sequencing Shared Resource (MPSSR) at Oregon Health and Science University (OHSU) for library preparation. Briefly, total RNA concentration and sample integrity was assessed via running on an Agilent RNA 6000 Pico chip on an Agilent Technologies 2100 Bioanalyzer instrument. Following quantification and quality control, 325ng of total RNA was subjected to ribosomal RNA reduction via Epicenter’s Ribo-Zero™ Gold kit (Human/Mouse/Rat). The output of this was taken into Illumina’s TruSeq® RNA Sample Preparation v2, beginning with the RNA fragmentation step. It should be noted that a poly(A) selection was not performed due to limited starting total RNA. Barcode indexing adapters were ligated and all 10 samples (5 control and 5 injured) were sequenced (100 bp single reads) on a single flow cell lane on the Illumina HiSeq 2500 Seqeuncer Illumina HiSeq 2500 gds 302436969 GEO Accession GSM2436969 SRX2440131 GSM2436970 SRP095530 SRS1874718 GSM2436970 RNA-Seq TRANSCRIPTOMIC cDNA After collecting 60-80 dissected VNCs on dry ice, the tissue wash crushed in 500ul of Trizol with glass beads and a pestle. This preparation was then stored at -80 degrees until all the samples were ready for RNA extraction. RNA extraction was performed by first spinning the crushed material at 11,000G for 10 minutes at 4 degrees C to pellet cuticle and lipids. Trizol supernatant was pulled off the pellet and 1/5 volume of chloroform was added and mixed in a 5’ Prime- heavy phase lock gel and centrifuged. The aqueous phase was pulled off and the RNA was cleaned and isolated on a Zymo Research RCC5 column. An on column DNase digestion was performed to degrade genomic DNA. The RNA was quantified using the Qubit fluorometer before sending total RNA samples to the Massively Parallel Sequencing Shared Resource (MPSSR) at Oregon Health and Science University (OHSU) for library preparation. Briefly, total RNA concentration and sample integrity was assessed via running on an Agilent RNA 6000 Pico chip on an Agilent Technologies 2100 Bioanalyzer instrument. Following quantification and quality control, 325ng of total RNA was subjected to ribosomal RNA reduction via Epicenter’s Ribo-Zero™ Gold kit (Human/Mouse/Rat). The output of this was taken into Illumina’s TruSeq® RNA Sample Preparation v2, beginning with the RNA fragmentation step. It should be noted that a poly(A) selection was not performed due to limited starting total RNA. Barcode indexing adapters were ligated and all 10 samples (5 control and 5 injured) were sequenced (100 bp single reads) on a single flow cell lane on the Illumina HiSeq 2500 Seqeuncer Illumina HiSeq 2500 gds 302436970 GEO Accession GSM2436970 SRX2440132 GSM2436971 SRP095530 SRS1874719 GSM2436971 RNA-Seq TRANSCRIPTOMIC cDNA After collecting 60-80 dissected VNCs on dry ice, the tissue wash crushed in 500ul of Trizol with glass beads and a pestle. This preparation was then stored at -80 degrees until all the samples were ready for RNA extraction. RNA extraction was performed by first spinning the crushed material at 11,000G for 10 minutes at 4 degrees C to pellet cuticle and lipids. Trizol supernatant was pulled off the pellet and 1/5 volume of chloroform was added and mixed in a 5’ Prime- heavy phase lock gel and centrifuged. The aqueous phase was pulled off and the RNA was cleaned and isolated on a Zymo Research RCC5 column. An on column DNase digestion was performed to degrade genomic DNA. The RNA was quantified using the Qubit fluorometer before sending total RNA samples to the Massively Parallel Sequencing Shared Resource (MPSSR) at Oregon Health and Science University (OHSU) for library preparation. Briefly, total RNA concentration and sample integrity was assessed via running on an Agilent RNA 6000 Pico chip on an Agilent Technologies 2100 Bioanalyzer instrument. Following quantification and quality control, 325ng of total RNA was subjected to ribosomal RNA reduction via Epicenter’s Ribo-Zero™ Gold kit (Human/Mouse/Rat). The output of this was taken into Illumina’s TruSeq® RNA Sample Preparation v2, beginning with the RNA fragmentation step. It should be noted that a poly(A) selection was not performed due to limited starting total RNA. Barcode indexing adapters were ligated and all 10 samples (5 control and 5 injured) were sequenced (100 bp single reads) on a single flow cell lane on the Illumina HiSeq 2500 Seqeuncer Illumina HiSeq 2500 gds 302436971 GEO Accession GSM2436971 SRX2440133 GSM2436972 SRP095530 SRS1874720 GSM2436972 RNA-Seq TRANSCRIPTOMIC cDNA After collecting 60-80 dissected VNCs on dry ice, the tissue wash crushed in 500ul of Trizol with glass beads and a pestle. This preparation was then stored at -80 degrees until all the samples were ready for RNA extraction. RNA extraction was performed by first spinning the crushed material at 11,000G for 10 minutes at 4 degrees C to pellet cuticle and lipids. Trizol supernatant was pulled off the pellet and 1/5 volume of chloroform was added and mixed in a 5’ Prime- heavy phase lock gel and centrifuged. The aqueous phase was pulled off and the RNA was cleaned and isolated on a Zymo Research RCC5 column. An on column DNase digestion was performed to degrade genomic DNA. The RNA was quantified using the Qubit fluorometer before sending total RNA samples to the Massively Parallel Sequencing Shared Resource (MPSSR) at Oregon Health and Science University (OHSU) for library preparation. Briefly, total RNA concentration and sample integrity was assessed via running on an Agilent RNA 6000 Pico chip on an Agilent Technologies 2100 Bioanalyzer instrument. Following quantification and quality control, 325ng of total RNA was subjected to ribosomal RNA reduction via Epicenter’s Ribo-Zero™ Gold kit (Human/Mouse/Rat). The output of this was taken into Illumina’s TruSeq® RNA Sample Preparation v2, beginning with the RNA fragmentation step. It should be noted that a poly(A) selection was not performed due to limited starting total RNA. Barcode indexing adapters were ligated and all 10 samples (5 control and 5 injured) were sequenced (100 bp single reads) on a single flow cell lane on the Illumina HiSeq 2500 Seqeuncer Illumina HiSeq 2500 gds 302436972 GEO Accession GSM2436972 SRX2440134 GSM2436973 SRP095530 SRS1874721 GSM2436973 RNA-Seq TRANSCRIPTOMIC cDNA After collecting 60-80 dissected VNCs on dry ice, the tissue wash crushed in 500ul of Trizol with glass beads and a pestle. This preparation was then stored at -80 degrees until all the samples were ready for RNA extraction. RNA extraction was performed by first spinning the crushed material at 11,000G for 10 minutes at 4 degrees C to pellet cuticle and lipids. Trizol supernatant was pulled off the pellet and 1/5 volume of chloroform was added and mixed in a 5’ Prime- heavy phase lock gel and centrifuged. The aqueous phase was pulled off and the RNA was cleaned and isolated on a Zymo Research RCC5 column. An on column DNase digestion was performed to degrade genomic DNA. The RNA was quantified using the Qubit fluorometer before sending total RNA samples to the Massively Parallel Sequencing Shared Resource (MPSSR) at Oregon Health and Science University (OHSU) for library preparation. Briefly, total RNA concentration and sample integrity was assessed via running on an Agilent RNA 6000 Pico chip on an Agilent Technologies 2100 Bioanalyzer instrument. Following quantification and quality control, 325ng of total RNA was subjected to ribosomal RNA reduction via Epicenter’s Ribo-Zero™ Gold kit (Human/Mouse/Rat). The output of this was taken into Illumina’s TruSeq® RNA Sample Preparation v2, beginning with the RNA fragmentation step. It should be noted that a poly(A) selection was not performed due to limited starting total RNA. Barcode indexing adapters were ligated and all 10 samples (5 control and 5 injured) were sequenced (100 bp single reads) on a single flow cell lane on the Illumina HiSeq 2500 Seqeuncer Illumina HiSeq 2500 gds 302436973 GEO Accession GSM2436973 SRX2440135 GSM2436974 SRP095530 SRS1874722 GSM2436974 RNA-Seq TRANSCRIPTOMIC cDNA After collecting 60-80 dissected VNCs on dry ice, the tissue wash crushed in 500ul of Trizol with glass beads and a pestle. This preparation was then stored at -80 degrees until all the samples were ready for RNA extraction. RNA extraction was performed by first spinning the crushed material at 11,000G for 10 minutes at 4 degrees C to pellet cuticle and lipids. Trizol supernatant was pulled off the pellet and 1/5 volume of chloroform was added and mixed in a 5’ Prime- heavy phase lock gel and centrifuged. The aqueous phase was pulled off and the RNA was cleaned and isolated on a Zymo Research RCC5 column. An on column DNase digestion was performed to degrade genomic DNA. The RNA was quantified using the Qubit fluorometer before sending total RNA samples to the Massively Parallel Sequencing Shared Resource (MPSSR) at Oregon Health and Science University (OHSU) for library preparation. Briefly, total RNA concentration and sample integrity was assessed via running on an Agilent RNA 6000 Pico chip on an Agilent Technologies 2100 Bioanalyzer instrument. Following quantification and quality control, 325ng of total RNA was subjected to ribosomal RNA reduction via Epicenter’s Ribo-Zero™ Gold kit (Human/Mouse/Rat). The output of this was taken into Illumina’s TruSeq® RNA Sample Preparation v2, beginning with the RNA fragmentation step. It should be noted that a poly(A) selection was not performed due to limited starting total RNA. Barcode indexing adapters were ligated and all 10 samples (5 control and 5 injured) were sequenced (100 bp single reads) on a single flow cell lane on the Illumina HiSeq 2500 Seqeuncer Illumina HiSeq 2500 gds 302436974 GEO Accession GSM2436974