SRX2449438 GSM2441722 SRP095835 SRS1885100 GSM2441722 RNA-Seq TRANSCRIPTOMIC cDNA Sampling from a CF animal and a sex-matched littermate control was performed within a time window of 20 min, and between 12:00-14:00h. Tissue was homogenized with a rotor-stator homogenizer in Trizol reagent (Qiagen), and total RNA was extracted using the Nucleospin RNA kit (Macherey-Nagel). Integrity of the extracted RNA was verified by gel electrophoresis. Libraries were prepared by BGI. In brief, the mRNA is enriched by using the oligo(dT) magnetic beads. Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then the first strand of cDNA is synthesized by using random hexamer-primer. Buffer, dNTPs, RNase H and DNA polymerase I are added to synthesize the second strand. The double strand cDNA is purified with magnetic beads. End reparation and 3’-end single nucleotide A (adenine) addition is then performed. Finally, sequencing adaptors are ligated to the fragments. The fragments are enriched by PCR amplification. During the QC step, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used to qualify and quantify of the sample library. The library products are ready for sequencing via Illumina HiSeqTM 2000 or Ion Proton System (4476610). Illumina HiSeq 2000 gds 302441722 GEO Accession GSM2441722 SRX2449439 GSM2441723 SRP095835 SRS1885098 GSM2441723 RNA-Seq TRANSCRIPTOMIC cDNA Sampling from a CF animal and a sex-matched littermate control was performed within a time window of 20 min, and between 12:00-14:00h. Tissue was homogenized with a rotor-stator homogenizer in Trizol reagent (Qiagen), and total RNA was extracted using the Nucleospin RNA kit (Macherey-Nagel). Integrity of the extracted RNA was verified by gel electrophoresis. Libraries were prepared by BGI. In brief, the mRNA is enriched by using the oligo(dT) magnetic beads. Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then the first strand of cDNA is synthesized by using random hexamer-primer. Buffer, dNTPs, RNase H and DNA polymerase I are added to synthesize the second strand. The double strand cDNA is purified with magnetic beads. End reparation and 3’-end single nucleotide A (adenine) addition is then performed. Finally, sequencing adaptors are ligated to the fragments. The fragments are enriched by PCR amplification. During the QC step, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used to qualify and quantify of the sample library. The library products are ready for sequencing via Illumina HiSeqTM 2000 or Ion Proton System (4476610). Illumina HiSeq 2000 gds 302441723 GEO Accession GSM2441723 SRX2449440 GSM2441724 SRP095835 SRS1885099 GSM2441724 RNA-Seq TRANSCRIPTOMIC cDNA Sampling from a CF animal and a sex-matched littermate control was performed within a time window of 20 min, and between 12:00-14:00h. Tissue was homogenized with a rotor-stator homogenizer in Trizol reagent (Qiagen), and total RNA was extracted using the Nucleospin RNA kit (Macherey-Nagel). Integrity of the extracted RNA was verified by gel electrophoresis. Libraries were prepared by BGI. In brief, the mRNA is enriched by using the oligo(dT) magnetic beads. Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then the first strand of cDNA is synthesized by using random hexamer-primer. Buffer, dNTPs, RNase H and DNA polymerase I are added to synthesize the second strand. The double strand cDNA is purified with magnetic beads. End reparation and 3’-end single nucleotide A (adenine) addition is then performed. Finally, sequencing adaptors are ligated to the fragments. The fragments are enriched by PCR amplification. During the QC step, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used to qualify and quantify of the sample library. The library products are ready for sequencing via Illumina HiSeqTM 2000 or Ion Proton System (4476610). Ion Torrent Proton gds 302441724 GEO Accession GSM2441724 SRX2449441 GSM2441726 SRP095835 SRS1885101 GSM2441726 RNA-Seq TRANSCRIPTOMIC cDNA Sampling from a CF animal and a sex-matched littermate control was performed within a time window of 20 min, and between 12:00-14:00h. Tissue was homogenized with a rotor-stator homogenizer in Trizol reagent (Qiagen), and total RNA was extracted using the Nucleospin RNA kit (Macherey-Nagel). Integrity of the extracted RNA was verified by gel electrophoresis. Libraries were prepared by BGI. In brief, the mRNA is enriched by using the oligo(dT) magnetic beads. Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then the first strand of cDNA is synthesized by using random hexamer-primer. Buffer, dNTPs, RNase H and DNA polymerase I are added to synthesize the second strand. The double strand cDNA is purified with magnetic beads. End reparation and 3’-end single nucleotide A (adenine) addition is then performed. Finally, sequencing adaptors are ligated to the fragments. The fragments are enriched by PCR amplification. During the QC step, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used to qualify and quantify of the sample library. The library products are ready for sequencing via Illumina HiSeqTM 2000 or Ion Proton System (4476610). Illumina HiSeq 2000 gds 302441726 GEO Accession GSM2441726 SRX2449442 GSM2441725 SRP095835 SRS1885102 GSM2441725 RNA-Seq TRANSCRIPTOMIC cDNA Sampling from a CF animal and a sex-matched littermate control was performed within a time window of 20 min, and between 12:00-14:00h. Tissue was homogenized with a rotor-stator homogenizer in Trizol reagent (Qiagen), and total RNA was extracted using the Nucleospin RNA kit (Macherey-Nagel). Integrity of the extracted RNA was verified by gel electrophoresis. Libraries were prepared by BGI. In brief, the mRNA is enriched by using the oligo(dT) magnetic beads. Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then the first strand of cDNA is synthesized by using random hexamer-primer. Buffer, dNTPs, RNase H and DNA polymerase I are added to synthesize the second strand. The double strand cDNA is purified with magnetic beads. End reparation and 3’-end single nucleotide A (adenine) addition is then performed. Finally, sequencing adaptors are ligated to the fragments. The fragments are enriched by PCR amplification. During the QC step, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used to qualify and quantify of the sample library. The library products are ready for sequencing via Illumina HiSeqTM 2000 or Ion Proton System (4476610). Illumina HiSeq 2000 gds 302441725 GEO Accession GSM2441725 SRX2449443 GSM2441727 SRP095835 SRS1885103 GSM2441727 RNA-Seq TRANSCRIPTOMIC cDNA Sampling from a CF animal and a sex-matched littermate control was performed within a time window of 20 min, and between 12:00-14:00h. Tissue was homogenized with a rotor-stator homogenizer in Trizol reagent (Qiagen), and total RNA was extracted using the Nucleospin RNA kit (Macherey-Nagel). Integrity of the extracted RNA was verified by gel electrophoresis. Libraries were prepared by BGI. In brief, the mRNA is enriched by using the oligo(dT) magnetic beads. Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then the first strand of cDNA is synthesized by using random hexamer-primer. Buffer, dNTPs, RNase H and DNA polymerase I are added to synthesize the second strand. The double strand cDNA is purified with magnetic beads. End reparation and 3’-end single nucleotide A (adenine) addition is then performed. Finally, sequencing adaptors are ligated to the fragments. The fragments are enriched by PCR amplification. During the QC step, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used to qualify and quantify of the sample library. The library products are ready for sequencing via Illumina HiSeqTM 2000 or Ion Proton System (4476610). Illumina HiSeq 2000 gds 302441727 GEO Accession GSM2441727 SRX2449444 GSM2441728 SRP095835 SRS1885104 GSM2441728 RNA-Seq TRANSCRIPTOMIC cDNA Sampling from a CF animal and a sex-matched littermate control was performed within a time window of 20 min, and between 12:00-14:00h. Tissue was homogenized with a rotor-stator homogenizer in Trizol reagent (Qiagen), and total RNA was extracted using the Nucleospin RNA kit (Macherey-Nagel). Integrity of the extracted RNA was verified by gel electrophoresis. Libraries were prepared by BGI. In brief, the mRNA is enriched by using the oligo(dT) magnetic beads. Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then the first strand of cDNA is synthesized by using random hexamer-primer. Buffer, dNTPs, RNase H and DNA polymerase I are added to synthesize the second strand. The double strand cDNA is purified with magnetic beads. End reparation and 3’-end single nucleotide A (adenine) addition is then performed. Finally, sequencing adaptors are ligated to the fragments. The fragments are enriched by PCR amplification. During the QC step, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used to qualify and quantify of the sample library. The library products are ready for sequencing via Illumina HiSeqTM 2000 or Ion Proton System (4476610). Ion Torrent Proton gds 302441728 GEO Accession GSM2441728 SRX2449445 GSM2441729 SRP095835 SRS1885105 GSM2441729 RNA-Seq TRANSCRIPTOMIC cDNA Sampling from a CF animal and a sex-matched littermate control was performed within a time window of 20 min, and between 12:00-14:00h. Tissue was homogenized with a rotor-stator homogenizer in Trizol reagent (Qiagen), and total RNA was extracted using the Nucleospin RNA kit (Macherey-Nagel). Integrity of the extracted RNA was verified by gel electrophoresis. Libraries were prepared by BGI. In brief, the mRNA is enriched by using the oligo(dT) magnetic beads. Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then the first strand of cDNA is synthesized by using random hexamer-primer. Buffer, dNTPs, RNase H and DNA polymerase I are added to synthesize the second strand. The double strand cDNA is purified with magnetic beads. End reparation and 3’-end single nucleotide A (adenine) addition is then performed. Finally, sequencing adaptors are ligated to the fragments. The fragments are enriched by PCR amplification. During the QC step, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used to qualify and quantify of the sample library. The library products are ready for sequencing via Illumina HiSeqTM 2000 or Ion Proton System (4476610). Illumina HiSeq 2000 gds 302441729 GEO Accession GSM2441729