SRX2517793 GSM2468331 SRP097787 SRS1940519 GSM2468331 RNA-Seq TRANSCRIPTOMIC cDNA Bone & marrow fraction were separated from tibia, femur and humeri of WT and Gfra2KO mice. After RBC lysis, mature hematopoietic cells were depleted using the EasyStep Mouse Hematopoietic Progenitor Cell Isolation Cocktail (Stem cell Technologies, 19756) according to the manufacturer’s protocol. The depleted cell population was stained for 20 minutes in PBS with 2 % FCS for LSK CD48-CD150+ markers. After dead cell exclusion using DAPI, pools of 30 viable LSK CD48-CD150+ cells were sorted using BD Influx™ cell sorter into 4 µl lysis buffer (0.5 U/µl SUPERase In RNase Inhibitor in 0.2 % (v/v) Triton-X-100) containing 12.5 mM DTT and 2.5 mM dNTP. RNAseq was performed following Smart-seq2 protocol (Picelli 2014) Pooled libraries of 4 replicates per sample were prepared using the Illumina Nextera XT DNA preparation kit & sequencing was performed on the Illumina HiSeq 4000 Illumina HiSeq 4000 gds 302468331 GEO Accession GSM2468331 SRX2517794 GSM2468332 SRP097787 SRS1940518 GSM2468332 RNA-Seq TRANSCRIPTOMIC cDNA Bone & marrow fraction were separated from tibia, femur and humeri of WT and Gfra2KO mice. After RBC lysis, mature hematopoietic cells were depleted using the EasyStep Mouse Hematopoietic Progenitor Cell Isolation Cocktail (Stem cell Technologies, 19756) according to the manufacturer’s protocol. The depleted cell population was stained for 20 minutes in PBS with 2 % FCS for LSK CD48-CD150+ markers. After dead cell exclusion using DAPI, pools of 30 viable LSK CD48-CD150+ cells were sorted using BD Influx™ cell sorter into 4 µl lysis buffer (0.5 U/µl SUPERase In RNase Inhibitor in 0.2 % (v/v) Triton-X-100) containing 12.5 mM DTT and 2.5 mM dNTP. RNAseq was performed following Smart-seq2 protocol (Picelli 2014) Pooled libraries of 4 replicates per sample were prepared using the Illumina Nextera XT DNA preparation kit & sequencing was performed on the Illumina HiSeq 4000 Illumina HiSeq 4000 gds 302468332 GEO Accession GSM2468332 SRX2517795 GSM2468333 SRP097787 SRS1940520 GSM2468333 RNA-Seq TRANSCRIPTOMIC cDNA Bone & marrow fraction were separated from tibia, femur and humeri of WT and Gfra2KO mice. After RBC lysis, mature hematopoietic cells were depleted using the EasyStep Mouse Hematopoietic Progenitor Cell Isolation Cocktail (Stem cell Technologies, 19756) according to the manufacturer’s protocol. The depleted cell population was stained for 20 minutes in PBS with 2 % FCS for LSK CD48-CD150+ markers. After dead cell exclusion using DAPI, pools of 30 viable LSK CD48-CD150+ cells were sorted using BD Influx™ cell sorter into 4 µl lysis buffer (0.5 U/µl SUPERase In RNase Inhibitor in 0.2 % (v/v) Triton-X-100) containing 12.5 mM DTT and 2.5 mM dNTP. RNAseq was performed following Smart-seq2 protocol (Picelli 2014) Pooled libraries of 4 replicates per sample were prepared using the Illumina Nextera XT DNA preparation kit & sequencing was performed on the Illumina HiSeq 4000 Illumina HiSeq 4000 gds 302468333 GEO Accession GSM2468333 SRX2517796 GSM2468334 SRP097787 SRS1940521 GSM2468334 RNA-Seq TRANSCRIPTOMIC cDNA Bone & marrow fraction were separated from tibia, femur and humeri of WT and Gfra2KO mice. After RBC lysis, mature hematopoietic cells were depleted using the EasyStep Mouse Hematopoietic Progenitor Cell Isolation Cocktail (Stem cell Technologies, 19756) according to the manufacturer’s protocol. The depleted cell population was stained for 20 minutes in PBS with 2 % FCS for LSK CD48-CD150+ markers. After dead cell exclusion using DAPI, pools of 30 viable LSK CD48-CD150+ cells were sorted using BD Influx™ cell sorter into 4 µl lysis buffer (0.5 U/µl SUPERase In RNase Inhibitor in 0.2 % (v/v) Triton-X-100) containing 12.5 mM DTT and 2.5 mM dNTP. RNAseq was performed following Smart-seq2 protocol (Picelli 2014) Pooled libraries of 4 replicates per sample were prepared using the Illumina Nextera XT DNA preparation kit & sequencing was performed on the Illumina HiSeq 4000 Illumina HiSeq 4000 gds 302468334 GEO Accession GSM2468334 SRX2517797 GSM2468335 SRP097787 SRS1940522 GSM2468335 RNA-Seq TRANSCRIPTOMIC cDNA Bone & marrow fraction were separated from tibia, femur and humeri of WT and Gfra2KO mice. After RBC lysis, mature hematopoietic cells were depleted using the EasyStep Mouse Hematopoietic Progenitor Cell Isolation Cocktail (Stem cell Technologies, 19756) according to the manufacturer’s protocol. The depleted cell population was stained for 20 minutes in PBS with 2 % FCS for LSK CD48-CD150+ markers. After dead cell exclusion using DAPI, pools of 30 viable LSK CD48-CD150+ cells were sorted using BD Influx™ cell sorter into 4 µl lysis buffer (0.5 U/µl SUPERase In RNase Inhibitor in 0.2 % (v/v) Triton-X-100) containing 12.5 mM DTT and 2.5 mM dNTP. RNAseq was performed following Smart-seq2 protocol (Picelli 2014) Pooled libraries of 4 replicates per sample were prepared using the Illumina Nextera XT DNA preparation kit & sequencing was performed on the Illumina HiSeq 4000 Illumina HiSeq 4000 gds 302468335 GEO Accession GSM2468335 SRX2517798 GSM2468336 SRP097787 SRS1940523 GSM2468336 RNA-Seq TRANSCRIPTOMIC cDNA Bone & marrow fraction were separated from tibia, femur and humeri of WT and Gfra2KO mice. After RBC lysis, mature hematopoietic cells were depleted using the EasyStep Mouse Hematopoietic Progenitor Cell Isolation Cocktail (Stem cell Technologies, 19756) according to the manufacturer’s protocol. The depleted cell population was stained for 20 minutes in PBS with 2 % FCS for LSK CD48-CD150+ markers. After dead cell exclusion using DAPI, pools of 30 viable LSK CD48-CD150+ cells were sorted using BD Influx™ cell sorter into 4 µl lysis buffer (0.5 U/µl SUPERase In RNase Inhibitor in 0.2 % (v/v) Triton-X-100) containing 12.5 mM DTT and 2.5 mM dNTP. RNAseq was performed following Smart-seq2 protocol (Picelli 2014) Pooled libraries of 4 replicates per sample were prepared using the Illumina Nextera XT DNA preparation kit & sequencing was performed on the Illumina HiSeq 4000 Illumina HiSeq 4000 gds 302468336 GEO Accession GSM2468336 SRX2517799 GSM2468337 SRP097787 SRS1940524 GSM2468337 RNA-Seq TRANSCRIPTOMIC cDNA Bone & marrow fraction were separated from tibia, femur and humeri of WT and Gfra2KO mice. After RBC lysis, mature hematopoietic cells were depleted using the EasyStep Mouse Hematopoietic Progenitor Cell Isolation Cocktail (Stem cell Technologies, 19756) according to the manufacturer’s protocol. The depleted cell population was stained for 20 minutes in PBS with 2 % FCS for LSK CD48-CD150+ markers. After dead cell exclusion using DAPI, pools of 30 viable LSK CD48-CD150+ cells were sorted using BD Influx™ cell sorter into 4 µl lysis buffer (0.5 U/µl SUPERase In RNase Inhibitor in 0.2 % (v/v) Triton-X-100) containing 12.5 mM DTT and 2.5 mM dNTP. RNAseq was performed following Smart-seq2 protocol (Picelli 2014) Pooled libraries of 4 replicates per sample were prepared using the Illumina Nextera XT DNA preparation kit & sequencing was performed on the Illumina HiSeq 4000 Illumina HiSeq 4000 gds 302468337 GEO Accession GSM2468337 SRX2517800 GSM2468338 SRP097787 SRS1940525 GSM2468338 RNA-Seq TRANSCRIPTOMIC cDNA Bone & marrow fraction were separated from tibia, femur and humeri of WT and Gfra2KO mice. After RBC lysis, mature hematopoietic cells were depleted using the EasyStep Mouse Hematopoietic Progenitor Cell Isolation Cocktail (Stem cell Technologies, 19756) according to the manufacturer’s protocol. The depleted cell population was stained for 20 minutes in PBS with 2 % FCS for LSK CD48-CD150+ markers. After dead cell exclusion using DAPI, pools of 30 viable LSK CD48-CD150+ cells were sorted using BD Influx™ cell sorter into 4 µl lysis buffer (0.5 U/µl SUPERase In RNase Inhibitor in 0.2 % (v/v) Triton-X-100) containing 12.5 mM DTT and 2.5 mM dNTP. RNAseq was performed following Smart-seq2 protocol (Picelli 2014) Pooled libraries of 4 replicates per sample were prepared using the Illumina Nextera XT DNA preparation kit & sequencing was performed on the Illumina HiSeq 4000 Illumina HiSeq 4000 gds 302468338 GEO Accession GSM2468338 SRX2517801 GSM2468339 SRP097787 SRS1940526 GSM2468339 RNA-Seq TRANSCRIPTOMIC cDNA Bone & marrow fraction were separated from tibia, femur and humeri of WT and Gfra2KO mice. After RBC lysis, mature hematopoietic cells were depleted using the EasyStep Mouse Hematopoietic Progenitor Cell Isolation Cocktail (Stem cell Technologies, 19756) according to the manufacturer’s protocol. The depleted cell population was stained for 20 minutes in PBS with 2 % FCS for LSK CD48-CD150+ markers. After dead cell exclusion using DAPI, pools of 30 viable LSK CD48-CD150+ cells were sorted using BD Influx™ cell sorter into 4 µl lysis buffer (0.5 U/µl SUPERase In RNase Inhibitor in 0.2 % (v/v) Triton-X-100) containing 12.5 mM DTT and 2.5 mM dNTP. RNAseq was performed following Smart-seq2 protocol (Picelli 2014) Pooled libraries of 4 replicates per sample were prepared using the Illumina Nextera XT DNA preparation kit & sequencing was performed on the Illumina HiSeq 4000 Illumina HiSeq 4000 gds 302468339 GEO Accession GSM2468339 SRX2517802 GSM2468340 SRP097787 SRS1940527 GSM2468340 RNA-Seq TRANSCRIPTOMIC cDNA Bone & marrow fraction were separated from tibia, femur and humeri of WT and Gfra2KO mice. After RBC lysis, mature hematopoietic cells were depleted using the EasyStep Mouse Hematopoietic Progenitor Cell Isolation Cocktail (Stem cell Technologies, 19756) according to the manufacturer’s protocol. The depleted cell population was stained for 20 minutes in PBS with 2 % FCS for LSK CD48-CD150+ markers. After dead cell exclusion using DAPI, pools of 30 viable LSK CD48-CD150+ cells were sorted using BD Influx™ cell sorter into 4 µl lysis buffer (0.5 U/µl SUPERase In RNase Inhibitor in 0.2 % (v/v) Triton-X-100) containing 12.5 mM DTT and 2.5 mM dNTP. RNAseq was performed following Smart-seq2 protocol (Picelli 2014) Pooled libraries of 4 replicates per sample were prepared using the Illumina Nextera XT DNA preparation kit & sequencing was performed on the Illumina HiSeq 4000 Illumina HiSeq 4000 gds 302468340 GEO Accession GSM2468340 SRX2517803 GSM2468341 SRP097787 SRS1940528 GSM2468341 RNA-Seq TRANSCRIPTOMIC cDNA Bone & marrow fraction were separated from tibia, femur and humeri of WT and Gfra2KO mice. After RBC lysis, mature hematopoietic cells were depleted using the EasyStep Mouse Hematopoietic Progenitor Cell Isolation Cocktail (Stem cell Technologies, 19756) according to the manufacturer’s protocol. The depleted cell population was stained for 20 minutes in PBS with 2 % FCS for LSK CD48-CD150+ markers. After dead cell exclusion using DAPI, pools of 30 viable LSK CD48-CD150+ cells were sorted using BD Influx™ cell sorter into 4 µl lysis buffer (0.5 U/µl SUPERase In RNase Inhibitor in 0.2 % (v/v) Triton-X-100) containing 12.5 mM DTT and 2.5 mM dNTP. RNAseq was performed following Smart-seq2 protocol (Picelli 2014) Pooled libraries of 4 replicates per sample were prepared using the Illumina Nextera XT DNA preparation kit & sequencing was performed on the Illumina HiSeq 4000 Illumina HiSeq 4000 gds 302468341 GEO Accession GSM2468341 SRX2517804 GSM2468342 SRP097787 SRS1940529 GSM2468342 RNA-Seq TRANSCRIPTOMIC cDNA Bone & marrow fraction were separated from tibia, femur and humeri of WT and Gfra2KO mice. After RBC lysis, mature hematopoietic cells were depleted using the EasyStep Mouse Hematopoietic Progenitor Cell Isolation Cocktail (Stem cell Technologies, 19756) according to the manufacturer’s protocol. The depleted cell population was stained for 20 minutes in PBS with 2 % FCS for LSK CD48-CD150+ markers. After dead cell exclusion using DAPI, pools of 30 viable LSK CD48-CD150+ cells were sorted using BD Influx™ cell sorter into 4 µl lysis buffer (0.5 U/µl SUPERase In RNase Inhibitor in 0.2 % (v/v) Triton-X-100) containing 12.5 mM DTT and 2.5 mM dNTP. RNAseq was performed following Smart-seq2 protocol (Picelli 2014) Pooled libraries of 4 replicates per sample were prepared using the Illumina Nextera XT DNA preparation kit & sequencing was performed on the Illumina HiSeq 4000 Illumina HiSeq 4000 gds 302468342 GEO Accession GSM2468342 SRX2517805 GSM2468343 SRP097787 SRS1940530 GSM2468343 RNA-Seq TRANSCRIPTOMIC cDNA Bone & marrow fraction were separated from tibia, femur and humeri of WT and Gfra2KO mice. After RBC lysis, mature hematopoietic cells were depleted using the EasyStep Mouse Hematopoietic Progenitor Cell Isolation Cocktail (Stem cell Technologies, 19756) according to the manufacturer’s protocol. The depleted cell population was stained for 20 minutes in PBS with 2 % FCS for LSK CD48-CD150+ markers. After dead cell exclusion using DAPI, pools of 30 viable LSK CD48-CD150+ cells were sorted using BD Influx™ cell sorter into 4 µl lysis buffer (0.5 U/µl SUPERase In RNase Inhibitor in 0.2 % (v/v) Triton-X-100) containing 12.5 mM DTT and 2.5 mM dNTP. RNAseq was performed following Smart-seq2 protocol (Picelli 2014) Pooled libraries of 4 replicates per sample were prepared using the Illumina Nextera XT DNA preparation kit & sequencing was performed on the Illumina HiSeq 4000 Illumina HiSeq 4000 gds 302468343 GEO Accession GSM2468343 SRX2517806 GSM2468344 SRP097787 SRS1940531 GSM2468344 RNA-Seq TRANSCRIPTOMIC cDNA Bone & marrow fraction were separated from tibia, femur and humeri of WT and Gfra2KO mice. After RBC lysis, mature hematopoietic cells were depleted using the EasyStep Mouse Hematopoietic Progenitor Cell Isolation Cocktail (Stem cell Technologies, 19756) according to the manufacturer’s protocol. The depleted cell population was stained for 20 minutes in PBS with 2 % FCS for LSK CD48-CD150+ markers. After dead cell exclusion using DAPI, pools of 30 viable LSK CD48-CD150+ cells were sorted using BD Influx™ cell sorter into 4 µl lysis buffer (0.5 U/µl SUPERase In RNase Inhibitor in 0.2 % (v/v) Triton-X-100) containing 12.5 mM DTT and 2.5 mM dNTP. RNAseq was performed following Smart-seq2 protocol (Picelli 2014) Pooled libraries of 4 replicates per sample were prepared using the Illumina Nextera XT DNA preparation kit & sequencing was performed on the Illumina HiSeq 4000 Illumina HiSeq 4000 gds 302468344 GEO Accession GSM2468344 SRX2517807 GSM2468345 SRP097787 SRS1940532 GSM2468345 RNA-Seq TRANSCRIPTOMIC cDNA Bone & marrow fraction were separated from tibia, femur and humeri of WT and Gfra2KO mice. After RBC lysis, mature hematopoietic cells were depleted using the EasyStep Mouse Hematopoietic Progenitor Cell Isolation Cocktail (Stem cell Technologies, 19756) according to the manufacturer’s protocol. The depleted cell population was stained for 20 minutes in PBS with 2 % FCS for LSK CD48-CD150+ markers. After dead cell exclusion using DAPI, pools of 30 viable LSK CD48-CD150+ cells were sorted using BD Influx™ cell sorter into 4 µl lysis buffer (0.5 U/µl SUPERase In RNase Inhibitor in 0.2 % (v/v) Triton-X-100) containing 12.5 mM DTT and 2.5 mM dNTP. RNAseq was performed following Smart-seq2 protocol (Picelli 2014) Pooled libraries of 4 replicates per sample were prepared using the Illumina Nextera XT DNA preparation kit & sequencing was performed on the Illumina HiSeq 4000 Illumina HiSeq 4000 gds 302468345 GEO Accession GSM2468345 SRX2517808 GSM2468346 SRP097787 SRS1940533 GSM2468346 RNA-Seq TRANSCRIPTOMIC cDNA Bone & marrow fraction were separated from tibia, femur and humeri of WT and Gfra2KO mice. After RBC lysis, mature hematopoietic cells were depleted using the EasyStep Mouse Hematopoietic Progenitor Cell Isolation Cocktail (Stem cell Technologies, 19756) according to the manufacturer’s protocol. The depleted cell population was stained for 20 minutes in PBS with 2 % FCS for LSK CD48-CD150+ markers. After dead cell exclusion using DAPI, pools of 30 viable LSK CD48-CD150+ cells were sorted using BD Influx™ cell sorter into 4 µl lysis buffer (0.5 U/µl SUPERase In RNase Inhibitor in 0.2 % (v/v) Triton-X-100) containing 12.5 mM DTT and 2.5 mM dNTP. RNAseq was performed following Smart-seq2 protocol (Picelli 2014) Pooled libraries of 4 replicates per sample were prepared using the Illumina Nextera XT DNA preparation kit & sequencing was performed on the Illumina HiSeq 4000 Illumina HiSeq 4000 gds 302468346 GEO Accession GSM2468346