SRX2564849 GSM2492305 SRP099882 SRS1981500 GSM2492305 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492305 GEO Accession GSM2492305 SRX2564850 GSM2492306 SRP099882 SRS1981501 GSM2492306 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492306 GEO Accession GSM2492306 SRX2564851 GSM2492307 SRP099882 SRS1981502 GSM2492307 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492307 GEO Accession GSM2492307 SRX2564852 GSM2492308 SRP099882 SRS1981503 GSM2492308 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492308 GEO Accession GSM2492308 SRX2564853 GSM2492309 SRP099882 SRS1981504 GSM2492309 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492309 GEO Accession GSM2492309 SRX2564854 GSM2492310 SRP099882 SRS1981505 GSM2492310 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492310 GEO Accession GSM2492310 SRX2564855 GSM2492311 SRP099882 SRS1981506 GSM2492311 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492311 GEO Accession GSM2492311 SRX2564856 GSM2492312 SRP099882 SRS1981507 GSM2492312 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492312 GEO Accession GSM2492312 SRX2564857 GSM2492313 SRP099882 SRS1981508 GSM2492313 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492313 GEO Accession GSM2492313 SRX2564858 GSM2492314 SRP099882 SRS1981509 GSM2492314 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492314 GEO Accession GSM2492314 SRX2564859 GSM2492315 SRP099882 SRS1981510 GSM2492315 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492315 GEO Accession GSM2492315 SRX2564860 GSM2492316 SRP099882 SRS1981512 GSM2492316 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492316 GEO Accession GSM2492316 SRX2564861 GSM2492317 SRP099882 SRS1981511 GSM2492317 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492317 GEO Accession GSM2492317 SRX2564862 GSM2492318 SRP099882 SRS1981513 GSM2492318 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492318 GEO Accession GSM2492318 SRX2564863 GSM2492319 SRP099882 SRS1981514 GSM2492319 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492319 GEO Accession GSM2492319 SRX2564864 GSM2492320 SRP099882 SRS1981515 GSM2492320 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492320 GEO Accession GSM2492320 SRX2564865 GSM2492321 SRP099882 SRS1981516 GSM2492321 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492321 GEO Accession GSM2492321 SRX2564866 GSM2492322 SRP099882 SRS1981517 GSM2492322 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492322 GEO Accession GSM2492322 SRX2564867 GSM2492323 SRP099882 SRS1981518 GSM2492323 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492323 GEO Accession GSM2492323 SRX2564868 GSM2492324 SRP099882 SRS1981519 GSM2492324 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492324 GEO Accession GSM2492324 SRX2564869 GSM2492325 SRP099882 SRS1981520 GSM2492325 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492325 GEO Accession GSM2492325 SRX2564870 GSM2492326 SRP099882 SRS1981521 GSM2492326 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492326 GEO Accession GSM2492326 SRX2564871 GSM2492327 SRP099882 SRS1981522 GSM2492327 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492327 GEO Accession GSM2492327 SRX2564872 GSM2492328 SRP099882 SRS1981523 GSM2492328 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492328 GEO Accession GSM2492328 SRX2564873 GSM2492329 SRP099882 SRS1981524 GSM2492329 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492329 GEO Accession GSM2492329 SRX2564874 GSM2492330 SRP099882 SRS1981525 GSM2492330 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492330 GEO Accession GSM2492330 SRX2564875 GSM2492331 SRP099882 SRS1981526 GSM2492331 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492331 GEO Accession GSM2492331 SRX2564876 GSM2492332 SRP099882 SRS1981527 GSM2492332 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492332 GEO Accession GSM2492332 SRX2564877 GSM2492333 SRP099882 SRS1981528 GSM2492333 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492333 GEO Accession GSM2492333 SRX2564878 GSM2492334 SRP099882 SRS1981529 GSM2492334 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492334 GEO Accession GSM2492334 SRX2564879 GSM2492335 SRP099882 SRS1981530 GSM2492335 RNA-Seq TRANSCRIPTOMIC cDNA Hearts were digested in RMPI containing 20mg/ML Liberase and 10U/ml DNase (37oC for 45 minutes). Single cell suspensions were filtered through a 100mM strainer and stained with antibodies to allow cDC1s,cDC2s, moDCs and MFs to be isolated by FACS. RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). Per sample, an amount of 4 ng of total RNA was used as input for the SMART-Seq v4 Ultra Low Input RNA protocol (version “040215”) from Clontech Laboratories, Inc. Subsequently, 1ng of purified cDNA was sheared to 300bp using the Covaris M220. From the sheared material, sequencing libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (version 3.0 -8/15), according to the manufacturer’s protocol including a size selection to 250bp insert size with the following minor modifications: only half of the library is subjected to a 15 cycle PCR in the final library amplification step. Sequence-libraries of each sample were equimolarly pooled and sequenced on 2 NextSeq500 flow-cells at 1x75 bp with a v2 high-output kit. NextSeq 500 gds 302492335 GEO Accession GSM2492335