SRX2566194 OMe_038 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982600 SAMN06340897 OMe_038 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566195 OMe_035 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982601 SAMN06340896 OMe_035 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566196 OMe_034 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982602 SAMN06340895 OMe_034 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566197 OMe_033 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982603 SAMN06340894 OMe_033 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566198 OMe_032 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982604 SAMN06340893 OMe_032 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566199 OMe_031 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982605 SAMN06340892 OMe_031 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566200 OMe_030 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982606 SAMN06340891 OMe_030 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566201 OMe_029 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982607 SAMN06340890 OMe_029 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566202 OMe_028 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982608 SAMN06340889 OMe_028 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566204 OMe_026 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982610 SAMN06340887 OMe_026 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566205 OMe_025 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982611 SAMN06340886 OMe_025 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566206 OMe_024 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982612 SAMN06340885 OMe_024 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566207 OMe_023 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982613 SAMN06340884 OMe_023 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566208 OMe_022 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982614 SAMN06340883 OMe_022 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566209 OMe_021 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982615 SAMN06340882 OMe_021 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566210 OMe_020 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982616 SAMN06340881 OMe_020 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566211 OMe_019 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982617 SAMN06340880 OMe_019 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566212 OMe_018 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982618 SAMN06340879 OMe_018 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566213 OMe_017 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982619 SAMN06340878 OMe_017 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566214 OMe_016 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982620 SAMN06340877 OMe_016 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566193 OMe_040 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982599 SAMN06340898 OMe_040 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566216 OMe_014 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982622 SAMN06340875 OMe_014 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566218 OMe_012 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982624 SAMN06340873 OMe_012 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566219 OMe_011 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982625 SAMN06340872 OMe_011 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566220 OMe_010 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982626 SAMN06340871 OMe_010 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566221 OMe_009 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982627 SAMN06340870 OMe_009 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566222 OMe_008 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982628 SAMN06340869 OMe_008 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566223 OMe_007 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982629 SAMN06340868 OMe_007 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566224 OMe_006 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982630 SAMN06340867 OMe_006 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566225 OMe_005 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982631 SAMN06340866 OMe_005 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566226 OMe_004 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982632 SAMN06340865 OMe_004 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566227 OMe_002 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982633 SAMN06340864 OMe_002 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566228 OMe_001 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982634 SAMN06340863 OMe_001 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566229 OMd_100 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982635 SAMN06340862 OMd_100 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566230 OMd_099 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982636 SAMN06340861 OMd_099 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566231 OMd_098 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982637 SAMN06340860 OMd_098 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566232 OMd_096 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982638 SAMN06340859 OMd_096 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566233 OMd_092 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982639 SAMN06340858 OMd_092 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566215 OMe_015 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982621 SAMN06340876 OMe_015 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566235 OMd_090 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982641 SAMN06340856 OMd_090 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566236 OMd_089 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982642 SAMN06340855 OMd_089 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566237 OMd_088 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982643 SAMN06340854 OMd_088 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566238 OMd_087 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982644 SAMN06340853 OMd_087 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566239 OMd_086 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982645 SAMN06340852 OMd_086 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566217 OMe_013 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982623 SAMN06340874 OMe_013 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566203 OMe_027 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982609 SAMN06340888 OMe_027 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566240 OMd_085 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982646 SAMN06340851 OMd_085 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566241 OMd_084 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982647 SAMN06340850 OMd_084 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566234 OMd_091 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982640 SAMN06340857 OMd_091 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566243 OMd_082 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982649 SAMN06340848 OMd_082 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566246 OMd_075 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982652 SAMN06340845 OMd_075 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566247 OMd_074 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982656 SAMN06340844 OMd_074 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566248 OMd_073 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982653 SAMN06340843 OMd_073 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566249 OMd_072 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982655 SAMN06340842 OMd_072 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566250 OMd_071 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982654 SAMN06340841 OMd_071 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566251 OMd_070 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982657 SAMN06340840 OMd_070 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566252 OMd_069 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982658 SAMN06340839 OMd_069 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566253 OMd_068 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982659 SAMN06340838 OMd_068 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566244 OMd_081 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982650 SAMN06340847 OMd_081 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566255 OMd_066 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982661 SAMN06340836 OMd_066 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566256 OMd_065 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982662 SAMN06340835 OMd_065 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566257 OMd_064 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982663 SAMN06340834 OMd_064 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566258 OMd_063 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982664 SAMN06340833 OMd_063 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566259 OMd_062 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982665 SAMN06340832 OMd_062 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566260 OMd_061 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982666 SAMN06340831 OMd_061 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566261 OMd_060 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982667 SAMN06340830 OMd_060 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566262 OMd_059 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982668 SAMN06340829 OMd_059 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566263 OMd_058 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982669 SAMN06340828 OMd_058 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566264 OMd_057 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982670 SAMN06340827 OMd_057 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566265 OMd_056 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982671 SAMN06340826 OMd_056 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566266 OMd_055 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982672 SAMN06340825 OMd_055 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566267 OMd_054 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982673 SAMN06340824 OMd_054 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566268 OMd_053 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982674 SAMN06340823 OMd_053 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566242 OMd_083 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982648 SAMN06340849 OMd_083 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566254 OMd_067 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982660 SAMN06340837 OMd_067 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566269 OMd_050 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982675 SAMN06340822 OMd_050 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566272 OMd_047 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982678 SAMN06340819 OMd_047 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566273 OMd_046 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982680 SAMN06340818 OMd_046 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566274 OMd_045 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982679 SAMN06340817 OMd_045 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566275 OMd_044 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982681 SAMN06340816 OMd_044 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566276 OMd_043 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982682 SAMN06340815 OMd_043 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566277 OMd_042 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982683 SAMN06340814 OMd_042 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566278 OMd_041 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982684 SAMN06340813 OMd_041 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566279 OMd_040 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982685 SAMN06340812 OMd_040 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566245 OMd_079 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982651 SAMN06340846 OMd_079 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566281 OMd_038 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982687 SAMN06340810 OMd_038 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566282 OMd_037 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982688 SAMN06340809 OMd_037 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566283 OMd_036 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982689 SAMN06340808 OMd_036 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566284 OMd_035 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982690 SAMN06340807 OMd_035 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566285 OMd_034 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982691 SAMN06340806 OMd_034 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566286 OMd_033 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982692 SAMN06340805 OMd_033 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566287 OMd_032 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982693 SAMN06340804 OMd_032 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566288 OMd_031 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982694 SAMN06340803 OMd_031 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566289 OMd_029 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982695 SAMN06340802 OMd_029 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566290 OMd_028 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982696 SAMN06340801 OMd_028 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566291 OMd_027 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982697 SAMN06340800 OMd_027 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566271 OMd_048 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982677 SAMN06340820 OMd_048 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566280 OMd_039 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982686 SAMN06340811 OMd_039 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566270 OMd_049 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982676 SAMN06340821 OMd_049 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566295 OMd_023 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982701 SAMN06340796 OMd_023 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566296 OMd_022 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982702 SAMN06340795 OMd_022 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566297 OMd_021 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982703 SAMN06340794 OMd_021 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566298 OMd_020 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982704 SAMN06340793 OMd_020 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566299 OMd_019 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982705 SAMN06340792 OMd_019 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566300 OMd_018 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982707 SAMN06340791 OMd_018 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566301 OMd_017 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982706 SAMN06340790 OMd_017 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566302 OMd_016 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982708 SAMN06340789 OMd_016 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566303 OMd_014 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982709 SAMN06340788 OMd_014 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566293 OMd_025 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982699 SAMN06340798 OMd_025 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566305 OMd_012 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982711 SAMN06340786 OMd_012 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566306 OMd_011 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982712 SAMN06340785 OMd_011 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566307 OMd_010 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982713 SAMN06340784 OMd_010 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566308 OMd_009 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982714 SAMN06340783 OMd_009 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566309 OMd_008 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982715 SAMN06340782 OMd_008 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566310 OMd_007 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982716 SAMN06340781 OMd_007 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566311 OMd_006 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982717 SAMN06340780 OMd_006 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566312 OMd_005 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982719 SAMN06340779 OMd_005 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566313 OMd_004 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982718 SAMN06340778 OMd_004 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566314 OMd_002 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982720 SAMN06340777 OMd_002 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566315 OMd_001 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982722 SAMN06340776 OMd_001 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566294 OMd_024 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982700 SAMN06340797 OMd_024 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566292 OMd_026 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982698 SAMN06340799 OMd_026 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566316 OMc_100 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982721 SAMN06340775 OMc_100 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566317 OMc_099 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982724 SAMN06340774 OMc_099 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566304 OMd_013 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982710 SAMN06340787 OMd_013 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566319 OMc_096 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982726 SAMN06340772 OMc_096 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566320 OMc_095 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982725 SAMN06340771 OMc_095 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566318 OMc_097 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982723 SAMN06340773 OMc_097 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566322 OMc_093 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982727 SAMN06340769 OMc_093 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566323 OMc_092 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982729 SAMN06340768 OMc_092 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566321 OMc_094 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982728 SAMN06340770 OMc_094 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566325 OMc_090 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982730 SAMN06340766 OMc_090 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566326 OMc_089 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982732 SAMN06340765 OMc_089 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566324 OMc_091 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982731 SAMN06340767 OMc_091 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566328 OMc_087 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982734 SAMN06340763 OMc_087 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566329 OMc_086 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982735 SAMN06340762 OMc_086 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566327 OMc_088 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982733 SAMN06340764 OMc_088 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566331 OMc_084 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982737 SAMN06340760 OMc_084 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566332 OMc_081 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982738 SAMN06340759 OMc_081 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566330 OMc_085 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982736 SAMN06340761 OMc_085 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566334 OMc_079 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982740 SAMN06340757 OMc_079 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566335 OMc_078 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982741 SAMN06340756 OMc_078 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566333 OMc_080 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982739 SAMN06340758 OMc_080 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566337 OMc_075 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982743 SAMN06340754 OMc_075 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566338 OMc_074 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982744 SAMN06340753 OMc_074 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566336 OMc_077 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982742 SAMN06340755 OMc_077 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566340 OMc_071 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982746 SAMN06340751 OMc_071 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566341 OMc_070 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982747 SAMN06340750 OMc_070 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566339 OMc_073 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982745 SAMN06340752 OMc_073 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566343 OMc_068 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982749 SAMN06340748 OMc_068 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566344 OMc_067 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982750 SAMN06340747 OMc_067 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566342 OMc_069 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982748 SAMN06340749 OMc_069 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566346 OMc_065 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982752 SAMN06340745 OMc_065 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566347 OMc_063 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982753 SAMN06340744 OMc_063 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566345 OMc_066 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982751 SAMN06340746 OMc_066 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566349 OMc_061 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982755 SAMN06340742 OMc_061 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566350 OMc_059 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982756 SAMN06340741 OMc_059 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566348 OMc_062 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982754 SAMN06340743 OMc_062 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566352 OMc_056 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982758 SAMN06340739 OMc_056 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566353 OMc_055 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982759 SAMN06340738 OMc_055 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566351 OMc_058 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982757 SAMN06340740 OMc_058 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566355 OMc_053 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982761 SAMN06340736 OMc_053 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566356 OMc_052 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982762 SAMN06340735 OMc_052 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566354 OMc_054 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982760 SAMN06340737 OMc_054 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566358 OMc_050 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982764 SAMN06340733 OMc_050 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566359 OMc_049 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982765 SAMN06340732 OMc_049 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566357 OMc_051 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982763 SAMN06340734 OMc_051 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566361 OMc_047 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982767 SAMN06340730 OMc_047 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566362 OMc_045 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982768 SAMN06340729 OMc_045 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566360 OMc_048 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982766 SAMN06340731 OMc_048 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566364 OMc_043 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982770 SAMN06340727 OMc_043 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566365 OMc_042 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982771 SAMN06340726 OMc_042 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566363 OMc_044 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982769 SAMN06340728 OMc_044 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566367 OMc_040 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982773 SAMN06340724 OMc_040 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566368 OMc_039 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982774 SAMN06340723 OMc_039 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566366 OMc_041 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982772 SAMN06340725 OMc_041 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566370 OMc_036 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982776 SAMN06340721 OMc_036 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566371 OMc_035 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982777 SAMN06340720 OMc_035 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566369 OMc_038 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982775 SAMN06340722 OMc_038 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566373 OMc_033 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982779 SAMN06340718 OMc_033 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566374 OMc_032 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982780 SAMN06340717 OMc_032 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566372 OMc_034 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982778 SAMN06340719 OMc_034 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566376 OMc_030 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982782 SAMN06340715 OMc_030 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566377 OMc_029 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982783 SAMN06340714 OMc_029 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566375 OMc_031 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982781 SAMN06340716 OMc_031 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566379 OMc_027 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982785 SAMN06340712 OMc_027 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566380 OMc_026 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982786 SAMN06340711 OMc_026 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566378 OMc_028 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982784 SAMN06340713 OMc_028 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566382 OMc_024 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982788 SAMN06340709 OMc_024 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566383 OMc_023 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982789 SAMN06340708 OMc_023 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566381 OMc_025 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982787 SAMN06340710 OMc_025 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566385 OMc_021 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982791 SAMN06340706 OMc_021 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566386 OMc_020 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982792 SAMN06340705 OMc_020 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566384 OMc_022 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982790 SAMN06340707 OMc_022 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566388 OMc_018 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982794 SAMN06340703 OMc_018 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566389 OMc_017 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982795 SAMN06340702 OMc_017 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566387 OMc_019 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982793 SAMN06340704 OMc_019 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566391 OMc_015 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982797 SAMN06340700 OMc_015 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566392 OMc_014 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982798 SAMN06340699 OMc_014 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566390 OMc_016 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982796 SAMN06340701 OMc_016 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566394 OMc_012 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982800 SAMN06340697 OMc_012 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566395 OMc_011 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982801 SAMN06340696 OMc_011 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566393 OMc_013 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982799 SAMN06340698 OMc_013 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566397 OMc_009 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982803 SAMN06340694 OMc_009 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566398 OMc_008 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982804 SAMN06340693 OMc_008 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566396 OMc_010 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982802 SAMN06340695 OMc_010 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566400 OMc_006 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982806 SAMN06340691 OMc_006 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566401 OMc_005 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982807 SAMN06340690 OMc_005 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566399 OMc_007 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982805 SAMN06340692 OMc_007 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566403 OMc_003 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982809 SAMN06340688 OMc_003 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566404 OMc_002 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982810 SAMN06340687 OMc_002 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566402 OMc_004 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982808 SAMN06340689 OMc_004 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566406 OMb_100 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982812 SAMN06340685 OMb_100 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566407 OMb_099 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982813 SAMN06340684 OMb_099 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566405 OMc_001 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982811 SAMN06340686 OMc_001 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566409 OMb_097 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982815 SAMN06340682 OMb_097 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566410 OMb_096 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982816 SAMN06340681 OMb_096 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566408 OMb_098 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982814 SAMN06340683 OMb_098 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566412 OMb_094 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982818 SAMN06340679 OMb_094 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566413 OMb_092 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982819 SAMN06340678 OMb_092 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566411 OMb_095 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982817 SAMN06340680 OMb_095 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566415 OMb_090 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982821 SAMN06340676 OMb_090 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566416 OMb_089 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982822 SAMN06340675 OMb_089 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566414 OMb_091 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982820 SAMN06340677 OMb_091 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566418 OMb_087 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982824 SAMN06340673 OMb_087 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566419 OMb_086 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982825 SAMN06340672 OMb_086 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566417 OMb_088 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982823 SAMN06340674 OMb_088 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566421 OMb_084 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982827 SAMN06340670 OMb_084 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566422 OMb_083 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982828 SAMN06340669 OMb_083 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566420 OMb_085 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982826 SAMN06340671 OMb_085 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566424 OMb_081 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982830 SAMN06340667 OMb_081 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566425 OMb_080 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982831 SAMN06340666 OMb_080 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566423 OMb_082 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982829 SAMN06340668 OMb_082 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566429 OMb_076 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982835 SAMN06340662 OMb_076 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566430 OMb_075 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982836 SAMN06340661 OMb_075 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566427 OMb_078 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982833 SAMN06340664 OMb_078 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566432 OMb_073 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982838 SAMN06340659 OMb_073 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566433 OMb_072 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982839 SAMN06340658 OMb_072 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566434 OMb_071 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982840 SAMN06340657 OMb_071 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566435 OMb_070 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982841 SAMN06340656 OMb_070 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566428 OMb_077 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982834 SAMN06340663 OMb_077 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566437 OMb_067 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982843 SAMN06340654 OMb_067 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566426 OMb_079 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982832 SAMN06340665 OMb_079 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566439 OMb_065 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982845 SAMN06340652 OMb_065 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566436 OMb_068 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982842 SAMN06340655 OMb_068 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566441 OMb_063 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982847 SAMN06340650 OMb_063 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566438 OMb_066 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982844 SAMN06340653 OMb_066 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566431 OMb_074 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982837 SAMN06340660 OMb_074 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566442 OMb_062 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982848 SAMN06340649 OMb_062 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566443 OMb_061 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982849 SAMN06340648 OMb_061 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566446 OMb_058 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982852 SAMN06340645 OMb_058 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566447 OMb_057 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982853 SAMN06340644 OMb_057 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566448 OMb_056 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982854 SAMN06340643 OMb_056 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566449 OMb_055 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982855 SAMN06340642 OMb_055 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566450 OMb_054 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982856 SAMN06340641 OMb_054 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566451 OMb_053 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982857 SAMN06340640 OMb_053 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566452 OMb_052 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982858 SAMN06340639 OMb_052 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566453 OMb_051 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982859 SAMN06340638 OMb_051 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566454 OMb_050 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982860 SAMN06340637 OMb_050 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566455 OMb_049 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982861 SAMN06340636 OMb_049 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566456 OMb_048 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982862 SAMN06340635 OMb_048 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566457 OMb_047 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982863 SAMN06340634 OMb_047 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566458 OMb_046 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982864 SAMN06340633 OMb_046 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566459 OMb_045 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982865 SAMN06340632 OMb_045 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566460 OMb_044 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982866 SAMN06340631 OMb_044 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566440 OMb_064 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982846 SAMN06340651 OMb_064 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566462 OMb_042 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982868 SAMN06340629 OMb_042 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566463 OMb_041 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982869 SAMN06340628 OMb_041 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566464 OMb_039 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982870 SAMN06340627 OMb_039 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566465 OMb_038 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982871 SAMN06340626 OMb_038 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566466 OMb_036 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982872 SAMN06340625 OMb_036 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566467 OMb_034 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982873 SAMN06340624 OMb_034 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566468 OMb_033 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982874 SAMN06340623 OMb_033 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566469 OMb_032 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982875 SAMN06340622 OMb_032 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566445 OMb_059 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982851 SAMN06340646 OMb_059 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566471 OMb_030 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982877 SAMN06340620 OMb_030 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566472 OMb_029 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982878 SAMN06340619 OMb_029 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566473 OMb_028 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982879 SAMN06340618 OMb_028 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566474 OMb_026 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982880 SAMN06340617 OMb_026 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566475 OMb_025 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982881 SAMN06340616 OMb_025 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566476 OMb_024 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982882 SAMN06340615 OMb_024 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566477 OMb_023 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982883 SAMN06340614 OMb_023 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566478 OMb_022 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982884 SAMN06340613 OMb_022 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566479 OMb_021 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982886 SAMN06340612 OMb_021 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566480 OMb_020 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982885 SAMN06340611 OMb_020 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566481 OMb_019 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982887 SAMN06340610 OMb_019 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566482 OMb_018 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982888 SAMN06340609 OMb_018 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566483 OMb_017 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982889 SAMN06340608 OMb_017 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566484 OMb_016 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982890 SAMN06340607 OMb_016 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566485 OMb_014 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982892 SAMN06340606 OMb_014 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566461 OMb_043 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982867 SAMN06340630 OMb_043 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566487 OMb_012 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982893 SAMN06340604 OMb_012 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566488 OMb_011 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982894 SAMN06340603 OMb_011 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566489 OMb_010 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982896 SAMN06340602 OMb_010 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566490 OMb_009 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982895 SAMN06340601 OMb_009 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566491 OMb_008 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982899 SAMN06340600 OMb_008 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566492 OMb_007 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982897 SAMN06340599 OMb_007 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566493 OMb_006 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982900 SAMN06340598 OMb_006 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566494 OMb_005 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982898 SAMN06340597 OMb_005 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566444 OMb_060 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982850 SAMN06340647 OMb_060 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566496 OMb_003 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982901 SAMN06340595 OMb_003 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566497 OMb_002 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982902 SAMN06340594 OMb_002 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566498 OMb_001 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982904 SAMN06340593 OMb_001 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566499 OMa_079 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982905 SAMN06340592 OMa_079 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566500 OMa_077 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982906 SAMN06340591 OMa_077 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566501 OMa_076 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982907 SAMN06340590 OMa_076 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566502 OMa_075 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982908 SAMN06340589 OMa_075 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566503 OMa_074 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982909 SAMN06340588 OMa_074 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566504 OMa_071 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982911 SAMN06340587 OMa_071 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566505 OMa_070 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982912 SAMN06340586 OMa_070 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566506 OMa_069 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982910 SAMN06340585 OMa_069 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566507 OMa_068 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982913 SAMN06340584 OMa_068 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566508 OMa_067 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982914 SAMN06340583 OMa_067 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566509 OMa_065 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982915 SAMN06340582 OMa_065 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566510 OMa_064 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982916 SAMN06340581 OMa_064 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566486 OMb_013 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982891 SAMN06340605 OMb_013 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566512 OMa_062 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982918 SAMN06340579 OMa_062 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566513 OMa_061 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982919 SAMN06340578 OMa_061 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566514 OMa_060 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982920 SAMN06340577 OMa_060 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566515 OMa_059 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982921 SAMN06340576 OMa_059 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566516 OMa_056 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982922 SAMN06340575 OMa_056 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566517 OMa_055 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982923 SAMN06340574 OMa_055 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566518 OMa_053 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982924 SAMN06340573 OMa_053 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566519 OMa_051 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982925 SAMN06340572 OMa_051 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566520 OMa_050 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982926 SAMN06340571 OMa_050 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566521 OMa_047 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982927 SAMN06340570 OMa_047 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566522 OMa_045 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982929 SAMN06340569 OMa_045 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566495 OMb_004 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982903 SAMN06340596 OMb_004 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566524 OMa_043 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982930 SAMN06340567 OMa_043 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566525 OMa_042 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982931 SAMN06340566 OMa_042 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566526 OMa_041 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982932 SAMN06340565 OMa_041 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566470 OMb_031 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982876 SAMN06340621 OMb_031 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566528 OMa_039 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982934 SAMN06340563 OMa_039 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566529 OMa_038 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982935 SAMN06340562 OMa_038 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566530 OMa_036 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982937 SAMN06340561 OMa_036 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566531 OMa_035 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982936 SAMN06340560 OMa_035 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566532 OMa_034 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982938 SAMN06340559 OMa_034 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566533 OMa_033 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982939 SAMN06340558 OMa_033 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566534 OMa_032 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982940 SAMN06340557 OMa_032 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566535 OMa_031 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982941 SAMN06340556 OMa_031 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566536 OMa_030 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982942 SAMN06340555 OMa_030 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566537 OMa_028 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982943 SAMN06340554 OMa_028 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566538 OMa_027 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982944 SAMN06340553 OMa_027 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566539 OMa_025 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982945 SAMN06340552 OMa_025 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566540 OMa_024 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982946 SAMN06340551 OMa_024 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566541 OMa_023 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982947 SAMN06340550 OMa_023 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566542 OMa_022 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982948 SAMN06340549 OMa_022 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566523 OMa_044 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982928 SAMN06340568 OMa_044 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566544 OMa_019 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982950 SAMN06340547 OMa_019 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566545 OMa_018 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982951 SAMN06340546 OMa_018 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566546 OMa_017 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982953 SAMN06340545 OMa_017 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566547 OMa_016 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982952 SAMN06340544 OMa_016 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566548 OMa_015 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982954 SAMN06340543 OMa_015 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566549 OMa_014 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982955 SAMN06340542 OMa_014 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566550 OMa_013 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982956 SAMN06340541 OMa_013 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566551 OMa_012 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982957 SAMN06340540 OMa_012 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566527 OMa_040 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982933 SAMN06340564 OMa_040 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566553 OMa_010 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982959 SAMN06340538 OMa_010 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566554 OMa_009 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982960 SAMN06340537 OMa_009 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566555 OMa_007 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982961 SAMN06340536 OMa_007 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566556 OMa_005 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982964 SAMN06340535 OMa_005 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566557 OMa_004 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982963 SAMN06340534 OMa_004 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566558 OMa_003 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982962 SAMN06340533 OMa_003 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566559 OMa_002 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982965 SAMN06340532 OMa_002 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566560 OMa_001 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982966 SAMN06340531 OMa_001 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566511 OMa_063 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982917 SAMN06340580 OMa_063 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566552 OMa_011 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982958 SAMN06340539 OMa_011 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium SRX2566543 OMa_021 SRP100020 PRJNA306572 For each sample, PCRs were performed in triplicate using the Qiagen Multiplex PCR kit, between 10 and 80 ng template DNA, a primer concentration of 0.5 然, and a final reaction volume of 25 無. PCRs were performed as follows: One cycle at 95 。 for 15 min, 27 cycles each at 95 。 for 30 s, 55 。 for 90 s, and 72 。 for 30 s, followed by a final extension step at 72 。 for 10 min. Triplicate PCRs for each sample were pooled and cleaned with the Agencourt AMPure XP magnetic bead system (Beckman Coulter, Brea, CA, USA), and subsequently underwent an indexing PCR to add Nextera XT barcoded sequencing adapters (Illumina) according to the manufacturer掇 instructions. Indexed PCR products were cleaned using the Invitrogen SequalPrep normalization plate kit (Thermo Fisher Scientific, Carlsbad, CA, USA) following the manufacturer掇 instructions and eluted at normalized concentrations (~4 nM) in 20 _l elution buffer and pooled in equimolar ratios. Pooled samples were quality checked on the BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) for presence of primer dimers. The library was sequenced at 8 pM with 10% phiX on the Illumina MiSeq, 2*300 bp paired-end version 3 chemistry according to the manufacturer掇 specifications. SRS1982949 SAMN06340548 OMa_021 AMPLICON METAGENOMIC PCR 454 GS FLX Titanium