SRX2566772 GSM2492623 SRP100060 SRS1983148 GSM2492623 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested using the RNEasy-Plus Kit (Qiagen;74136) and assessed for quality using the TapeStation (Agilent). Samples with RNA Integrity Number of 8 or greater were rRNA depleted using Ribo Minus (Invitrogen;K1550-04). rRNA-depleted samples were prepared using the TruSeq mRNA SamplePrep v2 kit (Illumina;RS-122-2001, RS-122-2002). The entire fraction of 0.1-3 ug of rRNA depleted total RNA was fragmented and copied into first-strand cDNA using reverse transcriptase and random primers. cDNA 3‘-ends were adenylated and adapters were ligated. One of the adapters had a 6-nucleotide barcode that was unique for each sample, allowing us to multiplex in a HiSeq flow cell (Illumina). Products were purified and enriched by PCR to create the final cDNA library. Libraries were checked for quality and quantity by TapeStation (Agilent) and qPCR using a library quantification kit for Illumina platforms (Kapa Biosystems;KK4835). They were clustered on the cBot (illumina) and sequenced 24 samples per lane on 6 lanes of a 50-cycle single end HiSeq 4000. HiSeq Control Software version 3.3.52 was used according to manufacturer’s protocols. Demultiplexing and Fastq file generation was done using bcl2fastq version 2.17.1.14. Illumina HiSeq 4000 gds 302492623 GEO Accession GSM2492623 SRX2566773 GSM2492624 SRP100060 SRS1983150 GSM2492624 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested using the RNEasy-Plus Kit (Qiagen;74136) and assessed for quality using the TapeStation (Agilent). Samples with RNA Integrity Number of 8 or greater were rRNA depleted using Ribo Minus (Invitrogen;K1550-04). rRNA-depleted samples were prepared using the TruSeq mRNA SamplePrep v2 kit (Illumina;RS-122-2001, RS-122-2002). The entire fraction of 0.1-3 ug of rRNA depleted total RNA was fragmented and copied into first-strand cDNA using reverse transcriptase and random primers. cDNA 3‘-ends were adenylated and adapters were ligated. One of the adapters had a 6-nucleotide barcode that was unique for each sample, allowing us to multiplex in a HiSeq flow cell (Illumina). Products were purified and enriched by PCR to create the final cDNA library. Libraries were checked for quality and quantity by TapeStation (Agilent) and qPCR using a library quantification kit for Illumina platforms (Kapa Biosystems;KK4835). They were clustered on the cBot (illumina) and sequenced 24 samples per lane on 6 lanes of a 50-cycle single end HiSeq 4000. HiSeq Control Software version 3.3.52 was used according to manufacturer’s protocols. Demultiplexing and Fastq file generation was done using bcl2fastq version 2.17.1.14. Illumina HiSeq 4000 gds 302492624 GEO Accession GSM2492624 SRX2566774 GSM2492625 SRP100060 SRS1983149 GSM2492625 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested using the RNEasy-Plus Kit (Qiagen;74136) and assessed for quality using the TapeStation (Agilent). Samples with RNA Integrity Number of 8 or greater were rRNA depleted using Ribo Minus (Invitrogen;K1550-04). rRNA-depleted samples were prepared using the TruSeq mRNA SamplePrep v2 kit (Illumina;RS-122-2001, RS-122-2002). The entire fraction of 0.1-3 ug of rRNA depleted total RNA was fragmented and copied into first-strand cDNA using reverse transcriptase and random primers. cDNA 3‘-ends were adenylated and adapters were ligated. One of the adapters had a 6-nucleotide barcode that was unique for each sample, allowing us to multiplex in a HiSeq flow cell (Illumina). Products were purified and enriched by PCR to create the final cDNA library. Libraries were checked for quality and quantity by TapeStation (Agilent) and qPCR using a library quantification kit for Illumina platforms (Kapa Biosystems;KK4835). They were clustered on the cBot (illumina) and sequenced 24 samples per lane on 6 lanes of a 50-cycle single end HiSeq 4000. HiSeq Control Software version 3.3.52 was used according to manufacturer’s protocols. Demultiplexing and Fastq file generation was done using bcl2fastq version 2.17.1.14. Illumina HiSeq 4000 gds 302492625 GEO Accession GSM2492625 SRX2566775 GSM2492626 SRP100060 SRS1983151 GSM2492626 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested using the RNEasy-Plus Kit (Qiagen;74136) and assessed for quality using the TapeStation (Agilent). Samples with RNA Integrity Number of 8 or greater were rRNA depleted using Ribo Minus (Invitrogen;K1550-04). rRNA-depleted samples were prepared using the TruSeq mRNA SamplePrep v2 kit (Illumina;RS-122-2001, RS-122-2002). The entire fraction of 0.1-3 ug of rRNA depleted total RNA was fragmented and copied into first-strand cDNA using reverse transcriptase and random primers. cDNA 3‘-ends were adenylated and adapters were ligated. One of the adapters had a 6-nucleotide barcode that was unique for each sample, allowing us to multiplex in a HiSeq flow cell (Illumina). Products were purified and enriched by PCR to create the final cDNA library. Libraries were checked for quality and quantity by TapeStation (Agilent) and qPCR using a library quantification kit for Illumina platforms (Kapa Biosystems;KK4835). They were clustered on the cBot (illumina) and sequenced 24 samples per lane on 6 lanes of a 50-cycle single end HiSeq 4000. HiSeq Control Software version 3.3.52 was used according to manufacturer’s protocols. Demultiplexing and Fastq file generation was done using bcl2fastq version 2.17.1.14. Illumina HiSeq 4000 gds 302492626 GEO Accession GSM2492626 SRX2566776 GSM2492627 SRP100060 SRS1983152 GSM2492627 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested using the RNEasy-Plus Kit (Qiagen;74136) and assessed for quality using the TapeStation (Agilent). Samples with RNA Integrity Number of 8 or greater were rRNA depleted using Ribo Minus (Invitrogen;K1550-04). rRNA-depleted samples were prepared using the TruSeq mRNA SamplePrep v2 kit (Illumina;RS-122-2001, RS-122-2002). The entire fraction of 0.1-3 ug of rRNA depleted total RNA was fragmented and copied into first-strand cDNA using reverse transcriptase and random primers. cDNA 3‘-ends were adenylated and adapters were ligated. One of the adapters had a 6-nucleotide barcode that was unique for each sample, allowing us to multiplex in a HiSeq flow cell (Illumina). Products were purified and enriched by PCR to create the final cDNA library. Libraries were checked for quality and quantity by TapeStation (Agilent) and qPCR using a library quantification kit for Illumina platforms (Kapa Biosystems;KK4835). They were clustered on the cBot (illumina) and sequenced 24 samples per lane on 6 lanes of a 50-cycle single end HiSeq 4000. HiSeq Control Software version 3.3.52 was used according to manufacturer’s protocols. Demultiplexing and Fastq file generation was done using bcl2fastq version 2.17.1.14. Illumina HiSeq 4000 gds 302492627 GEO Accession GSM2492627 SRX2566777 GSM2492628 SRP100060 SRS1983153 GSM2492628 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested using the RNEasy-Plus Kit (Qiagen;74136) and assessed for quality using the TapeStation (Agilent). Samples with RNA Integrity Number of 8 or greater were rRNA depleted using Ribo Minus (Invitrogen;K1550-04). rRNA-depleted samples were prepared using the TruSeq mRNA SamplePrep v2 kit (Illumina;RS-122-2001, RS-122-2002). The entire fraction of 0.1-3 ug of rRNA depleted total RNA was fragmented and copied into first-strand cDNA using reverse transcriptase and random primers. cDNA 3‘-ends were adenylated and adapters were ligated. One of the adapters had a 6-nucleotide barcode that was unique for each sample, allowing us to multiplex in a HiSeq flow cell (Illumina). Products were purified and enriched by PCR to create the final cDNA library. Libraries were checked for quality and quantity by TapeStation (Agilent) and qPCR using a library quantification kit for Illumina platforms (Kapa Biosystems;KK4835). They were clustered on the cBot (illumina) and sequenced 24 samples per lane on 6 lanes of a 50-cycle single end HiSeq 4000. HiSeq Control Software version 3.3.52 was used according to manufacturer’s protocols. Demultiplexing and Fastq file generation was done using bcl2fastq version 2.17.1.14. Illumina HiSeq 4000 gds 302492628 GEO Accession GSM2492628 SRX2566778 GSM2492629 SRP100060 SRS1983154 GSM2492629 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested using the RNEasy-Plus Kit (Qiagen;74136) and assessed for quality using the TapeStation (Agilent). Samples with RNA Integrity Number of 8 or greater were rRNA depleted using Ribo Minus (Invitrogen;K1550-04). rRNA-depleted samples were prepared using the TruSeq mRNA SamplePrep v2 kit (Illumina;RS-122-2001, RS-122-2002). The entire fraction of 0.1-3 ug of rRNA depleted total RNA was fragmented and copied into first-strand cDNA using reverse transcriptase and random primers. cDNA 3‘-ends were adenylated and adapters were ligated. One of the adapters had a 6-nucleotide barcode that was unique for each sample, allowing us to multiplex in a HiSeq flow cell (Illumina). Products were purified and enriched by PCR to create the final cDNA library. Libraries were checked for quality and quantity by TapeStation (Agilent) and qPCR using a library quantification kit for Illumina platforms (Kapa Biosystems;KK4835). They were clustered on the cBot (illumina) and sequenced 24 samples per lane on 6 lanes of a 50-cycle single end HiSeq 4000. HiSeq Control Software version 3.3.52 was used according to manufacturer’s protocols. Demultiplexing and Fastq file generation was done using bcl2fastq version 2.17.1.14. Illumina HiSeq 4000 gds 302492629 GEO Accession GSM2492629 SRX2566779 GSM2492630 SRP100060 SRS1983155 GSM2492630 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested using the RNEasy-Plus Kit (Qiagen;74136) and assessed for quality using the TapeStation (Agilent). Samples with RNA Integrity Number of 8 or greater were rRNA depleted using Ribo Minus (Invitrogen;K1550-04). rRNA-depleted samples were prepared using the TruSeq mRNA SamplePrep v2 kit (Illumina;RS-122-2001, RS-122-2002). The entire fraction of 0.1-3 ug of rRNA depleted total RNA was fragmented and copied into first-strand cDNA using reverse transcriptase and random primers. cDNA 3‘-ends were adenylated and adapters were ligated. One of the adapters had a 6-nucleotide barcode that was unique for each sample, allowing us to multiplex in a HiSeq flow cell (Illumina). Products were purified and enriched by PCR to create the final cDNA library. Libraries were checked for quality and quantity by TapeStation (Agilent) and qPCR using a library quantification kit for Illumina platforms (Kapa Biosystems;KK4835). They were clustered on the cBot (illumina) and sequenced 24 samples per lane on 6 lanes of a 50-cycle single end HiSeq 4000. HiSeq Control Software version 3.3.52 was used according to manufacturer’s protocols. Demultiplexing and Fastq file generation was done using bcl2fastq version 2.17.1.14. Illumina HiSeq 4000 gds 302492630 GEO Accession GSM2492630 SRX2566780 GSM2492631 SRP100060 SRS1983156 GSM2492631 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested using the RNEasy-Plus Kit (Qiagen;74136) and assessed for quality using the TapeStation (Agilent). Samples with RNA Integrity Number of 8 or greater were rRNA depleted using Ribo Minus (Invitrogen;K1550-04). rRNA-depleted samples were prepared using the TruSeq mRNA SamplePrep v2 kit (Illumina;RS-122-2001, RS-122-2002). The entire fraction of 0.1-3 ug of rRNA depleted total RNA was fragmented and copied into first-strand cDNA using reverse transcriptase and random primers. cDNA 3‘-ends were adenylated and adapters were ligated. One of the adapters had a 6-nucleotide barcode that was unique for each sample, allowing us to multiplex in a HiSeq flow cell (Illumina). Products were purified and enriched by PCR to create the final cDNA library. Libraries were checked for quality and quantity by TapeStation (Agilent) and qPCR using a library quantification kit for Illumina platforms (Kapa Biosystems;KK4835). They were clustered on the cBot (illumina) and sequenced 24 samples per lane on 6 lanes of a 50-cycle single end HiSeq 4000. HiSeq Control Software version 3.3.52 was used according to manufacturer’s protocols. Demultiplexing and Fastq file generation was done using bcl2fastq version 2.17.1.14. Illumina HiSeq 4000 gds 302492631 GEO Accession GSM2492631 SRX2566781 GSM2492632 SRP100060 SRS1983157 GSM2492632 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested using the RNEasy-Plus Kit (Qiagen;74136) and assessed for quality using the TapeStation (Agilent). Samples with RNA Integrity Number of 8 or greater were rRNA depleted using Ribo Minus (Invitrogen;K1550-04). rRNA-depleted samples were prepared using the TruSeq mRNA SamplePrep v2 kit (Illumina;RS-122-2001, RS-122-2002). The entire fraction of 0.1-3 ug of rRNA depleted total RNA was fragmented and copied into first-strand cDNA using reverse transcriptase and random primers. cDNA 3‘-ends were adenylated and adapters were ligated. One of the adapters had a 6-nucleotide barcode that was unique for each sample, allowing us to multiplex in a HiSeq flow cell (Illumina). Products were purified and enriched by PCR to create the final cDNA library. Libraries were checked for quality and quantity by TapeStation (Agilent) and qPCR using a library quantification kit for Illumina platforms (Kapa Biosystems;KK4835). They were clustered on the cBot (illumina) and sequenced 24 samples per lane on 6 lanes of a 50-cycle single end HiSeq 4000. HiSeq Control Software version 3.3.52 was used according to manufacturer’s protocols. Demultiplexing and Fastq file generation was done using bcl2fastq version 2.17.1.14. Illumina HiSeq 4000 gds 302492632 GEO Accession GSM2492632 SRX2566782 GSM2492633 SRP100060 SRS1983158 GSM2492633 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested using the RNEasy-Plus Kit (Qiagen;74136) and assessed for quality using the TapeStation (Agilent). Samples with RNA Integrity Number of 8 or greater were rRNA depleted using Ribo Minus (Invitrogen;K1550-04). rRNA-depleted samples were prepared using the TruSeq mRNA SamplePrep v2 kit (Illumina;RS-122-2001, RS-122-2002). The entire fraction of 0.1-3 ug of rRNA depleted total RNA was fragmented and copied into first-strand cDNA using reverse transcriptase and random primers. cDNA 3‘-ends were adenylated and adapters were ligated. One of the adapters had a 6-nucleotide barcode that was unique for each sample, allowing us to multiplex in a HiSeq flow cell (Illumina). Products were purified and enriched by PCR to create the final cDNA library. Libraries were checked for quality and quantity by TapeStation (Agilent) and qPCR using a library quantification kit for Illumina platforms (Kapa Biosystems;KK4835). They were clustered on the cBot (illumina) and sequenced 24 samples per lane on 6 lanes of a 50-cycle single end HiSeq 4000. HiSeq Control Software version 3.3.52 was used according to manufacturer’s protocols. Demultiplexing and Fastq file generation was done using bcl2fastq version 2.17.1.14. Illumina HiSeq 4000 gds 302492633 GEO Accession GSM2492633 SRX2566783 GSM2492634 SRP100060 SRS1983159 GSM2492634 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested using the RNEasy-Plus Kit (Qiagen;74136) and assessed for quality using the TapeStation (Agilent). Samples with RNA Integrity Number of 8 or greater were rRNA depleted using Ribo Minus (Invitrogen;K1550-04). rRNA-depleted samples were prepared using the TruSeq mRNA SamplePrep v2 kit (Illumina;RS-122-2001, RS-122-2002). The entire fraction of 0.1-3 ug of rRNA depleted total RNA was fragmented and copied into first-strand cDNA using reverse transcriptase and random primers. cDNA 3‘-ends were adenylated and adapters were ligated. One of the adapters had a 6-nucleotide barcode that was unique for each sample, allowing us to multiplex in a HiSeq flow cell (Illumina). Products were purified and enriched by PCR to create the final cDNA library. Libraries were checked for quality and quantity by TapeStation (Agilent) and qPCR using a library quantification kit for Illumina platforms (Kapa Biosystems;KK4835). They were clustered on the cBot (illumina) and sequenced 24 samples per lane on 6 lanes of a 50-cycle single end HiSeq 4000. HiSeq Control Software version 3.3.52 was used according to manufacturer’s protocols. Demultiplexing and Fastq file generation was done using bcl2fastq version 2.17.1.14. Illumina HiSeq 4000 gds 302492634 GEO Accession GSM2492634 SRX2566784 GSM2492635 SRP100060 SRS1983160 GSM2492635 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested using the RNEasy-Plus Kit (Qiagen;74136) and assessed for quality using the TapeStation (Agilent). Samples with RNA Integrity Number of 8 or greater were rRNA depleted using Ribo Minus (Invitrogen;K1550-04). rRNA-depleted samples were prepared using the TruSeq mRNA SamplePrep v2 kit (Illumina;RS-122-2001, RS-122-2002). The entire fraction of 0.1-3 ug of rRNA depleted total RNA was fragmented and copied into first-strand cDNA using reverse transcriptase and random primers. cDNA 3‘-ends were adenylated and adapters were ligated. One of the adapters had a 6-nucleotide barcode that was unique for each sample, allowing us to multiplex in a HiSeq flow cell (Illumina). Products were purified and enriched by PCR to create the final cDNA library. Libraries were checked for quality and quantity by TapeStation (Agilent) and qPCR using a library quantification kit for Illumina platforms (Kapa Biosystems;KK4835). They were clustered on the cBot (illumina) and sequenced 24 samples per lane on 6 lanes of a 50-cycle single end HiSeq 4000. HiSeq Control Software version 3.3.52 was used according to manufacturer’s protocols. Demultiplexing and Fastq file generation was done using bcl2fastq version 2.17.1.14. Illumina HiSeq 4000 gds 302492635 GEO Accession GSM2492635 SRX2566785 GSM2492636 SRP100060 SRS1983161 GSM2492636 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested using the RNEasy-Plus Kit (Qiagen;74136) and assessed for quality using the TapeStation (Agilent). Samples with RNA Integrity Number of 8 or greater were rRNA depleted using Ribo Minus (Invitrogen;K1550-04). rRNA-depleted samples were prepared using the TruSeq mRNA SamplePrep v2 kit (Illumina;RS-122-2001, RS-122-2002). The entire fraction of 0.1-3 ug of rRNA depleted total RNA was fragmented and copied into first-strand cDNA using reverse transcriptase and random primers. cDNA 3‘-ends were adenylated and adapters were ligated. One of the adapters had a 6-nucleotide barcode that was unique for each sample, allowing us to multiplex in a HiSeq flow cell (Illumina). Products were purified and enriched by PCR to create the final cDNA library. Libraries were checked for quality and quantity by TapeStation (Agilent) and qPCR using a library quantification kit for Illumina platforms (Kapa Biosystems;KK4835). They were clustered on the cBot (illumina) and sequenced 24 samples per lane on 6 lanes of a 50-cycle single end HiSeq 4000. HiSeq Control Software version 3.3.52 was used according to manufacturer’s protocols. Demultiplexing and Fastq file generation was done using bcl2fastq version 2.17.1.14. Illumina HiSeq 4000 gds 302492636 GEO Accession GSM2492636 SRX2566786 GSM2492637 SRP100060 SRS1983162 GSM2492637 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested using the RNEasy-Plus Kit (Qiagen;74136) and assessed for quality using the TapeStation (Agilent). Samples with RNA Integrity Number of 8 or greater were rRNA depleted using Ribo Minus (Invitrogen;K1550-04). rRNA-depleted samples were prepared using the TruSeq mRNA SamplePrep v2 kit (Illumina;RS-122-2001, RS-122-2002). The entire fraction of 0.1-3 ug of rRNA depleted total RNA was fragmented and copied into first-strand cDNA using reverse transcriptase and random primers. cDNA 3‘-ends were adenylated and adapters were ligated. One of the adapters had a 6-nucleotide barcode that was unique for each sample, allowing us to multiplex in a HiSeq flow cell (Illumina). Products were purified and enriched by PCR to create the final cDNA library. Libraries were checked for quality and quantity by TapeStation (Agilent) and qPCR using a library quantification kit for Illumina platforms (Kapa Biosystems;KK4835). They were clustered on the cBot (illumina) and sequenced 24 samples per lane on 6 lanes of a 50-cycle single end HiSeq 4000. HiSeq Control Software version 3.3.52 was used according to manufacturer’s protocols. Demultiplexing and Fastq file generation was done using bcl2fastq version 2.17.1.14. Illumina HiSeq 4000 gds 302492637 GEO Accession GSM2492637 SRX2566787 GSM2492638 SRP100060 SRS1983163 GSM2492638 RNA-Seq TRANSCRIPTOMIC cDNA RNA was harvested using the RNEasy-Plus Kit (Qiagen;74136) and assessed for quality using the TapeStation (Agilent). Samples with RNA Integrity Number of 8 or greater were rRNA depleted using Ribo Minus (Invitrogen;K1550-04). rRNA-depleted samples were prepared using the TruSeq mRNA SamplePrep v2 kit (Illumina;RS-122-2001, RS-122-2002). The entire fraction of 0.1-3 ug of rRNA depleted total RNA was fragmented and copied into first-strand cDNA using reverse transcriptase and random primers. cDNA 3‘-ends were adenylated and adapters were ligated. One of the adapters had a 6-nucleotide barcode that was unique for each sample, allowing us to multiplex in a HiSeq flow cell (Illumina). Products were purified and enriched by PCR to create the final cDNA library. Libraries were checked for quality and quantity by TapeStation (Agilent) and qPCR using a library quantification kit for Illumina platforms (Kapa Biosystems;KK4835). They were clustered on the cBot (illumina) and sequenced 24 samples per lane on 6 lanes of a 50-cycle single end HiSeq 4000. HiSeq Control Software version 3.3.52 was used according to manufacturer’s protocols. Demultiplexing and Fastq file generation was done using bcl2fastq version 2.17.1.14. Illumina HiSeq 4000 gds 302492638 GEO Accession GSM2492638