SRX2568511 GSM2494232 SRP100217 SRS1984866 GSM2494232 RNA-Seq TRANSCRIPTOMIC cDNA The mpkCCD cells grown on permeable inserts of 6-well transwell were washed with PBS and lysed with 100 ul Trizol solution. Total RNA was isolated using Direct-zol RNA mini prep plus (ZYMO RESEARCH, R2070) as manufacturer's protocol. Sequencing libraries were prepared using Illumina TruSeq Stranded Total RNA Sample Prep Kit (Cat#RS-122-2201) as manufacturer's instruction. Total RNA (262 ng) of each sample was applied to ribosomal RNA removal beads to deplete ribosomal RNAs. The purified RNA was fragmented and then primed with random hexamers for cDNA synthesis using reverse transcriptase and random primers. First strand cDNA and second strand cDNA were sythesized serially and ligated with indexing adapters. DNA fragments including adapters were amplified by PCR using a PCR primer cocktail. Sample libraries were qualified using Agilent Technologies Bioanalyzer. Illumina HiSeq 3000 gds 302494232 GEO Accession GSM2494232 SRX2568512 GSM2494233 SRP100217 SRS1984867 GSM2494233 RNA-Seq TRANSCRIPTOMIC cDNA The mpkCCD cells grown on permeable inserts of 6-well transwell were washed with PBS and lysed with 100 ul Trizol solution. Total RNA was isolated using Direct-zol RNA mini prep plus (ZYMO RESEARCH, R2070) as manufacturer's protocol. Sequencing libraries were prepared using Illumina TruSeq Stranded Total RNA Sample Prep Kit (Cat#RS-122-2201) as manufacturer's instruction. Total RNA (262 ng) of each sample was applied to ribosomal RNA removal beads to deplete ribosomal RNAs. The purified RNA was fragmented and then primed with random hexamers for cDNA synthesis using reverse transcriptase and random primers. First strand cDNA and second strand cDNA were sythesized serially and ligated with indexing adapters. DNA fragments including adapters were amplified by PCR using a PCR primer cocktail. Sample libraries were qualified using Agilent Technologies Bioanalyzer. Illumina HiSeq 3000 gds 302494233 GEO Accession GSM2494233 SRX2568513 GSM2494234 SRP100217 SRS1984868 GSM2494234 RNA-Seq TRANSCRIPTOMIC cDNA The mpkCCD cells grown on permeable inserts of 6-well transwell were washed with PBS and lysed with 100 ul Trizol solution. Total RNA was isolated using Direct-zol RNA mini prep plus (ZYMO RESEARCH, R2070) as manufacturer's protocol. Sequencing libraries were prepared using Illumina TruSeq Stranded Total RNA Sample Prep Kit (Cat#RS-122-2201) as manufacturer's instruction. Total RNA (262 ng) of each sample was applied to ribosomal RNA removal beads to deplete ribosomal RNAs. The purified RNA was fragmented and then primed with random hexamers for cDNA synthesis using reverse transcriptase and random primers. First strand cDNA and second strand cDNA were sythesized serially and ligated with indexing adapters. DNA fragments including adapters were amplified by PCR using a PCR primer cocktail. Sample libraries were qualified using Agilent Technologies Bioanalyzer. Illumina HiSeq 3000 gds 302494234 GEO Accession GSM2494234 SRX2568514 GSM2494235 SRP100217 SRS1984869 GSM2494235 RNA-Seq TRANSCRIPTOMIC cDNA The mpkCCD cells grown on permeable inserts of 6-well transwell were washed with PBS and lysed with 100 ul Trizol solution. Total RNA was isolated using Direct-zol RNA mini prep plus (ZYMO RESEARCH, R2070) as manufacturer's protocol. Sequencing libraries were prepared using Illumina TruSeq Stranded Total RNA Sample Prep Kit (Cat#RS-122-2201) as manufacturer's instruction. Total RNA (262 ng) of each sample was applied to ribosomal RNA removal beads to deplete ribosomal RNAs. The purified RNA was fragmented and then primed with random hexamers for cDNA synthesis using reverse transcriptase and random primers. First strand cDNA and second strand cDNA were sythesized serially and ligated with indexing adapters. DNA fragments including adapters were amplified by PCR using a PCR primer cocktail. Sample libraries were qualified using Agilent Technologies Bioanalyzer. Illumina HiSeq 3000 gds 302494235 GEO Accession GSM2494235 SRX2568515 GSM2494236 SRP100217 SRS1984870 GSM2494236 RNA-Seq TRANSCRIPTOMIC cDNA The mpkCCD cells grown on permeable inserts of 6-well transwell were washed with PBS and lysed with 100 ul Trizol solution. Total RNA was isolated using Direct-zol RNA mini prep plus (ZYMO RESEARCH, R2070) as manufacturer's protocol. Sequencing libraries were prepared using Illumina TruSeq Stranded Total RNA Sample Prep Kit (Cat#RS-122-2201) as manufacturer's instruction. Total RNA (262 ng) of each sample was applied to ribosomal RNA removal beads to deplete ribosomal RNAs. The purified RNA was fragmented and then primed with random hexamers for cDNA synthesis using reverse transcriptase and random primers. First strand cDNA and second strand cDNA were sythesized serially and ligated with indexing adapters. DNA fragments including adapters were amplified by PCR using a PCR primer cocktail. Sample libraries were qualified using Agilent Technologies Bioanalyzer. Illumina HiSeq 3000 gds 302494236 GEO Accession GSM2494236 SRX2568516 GSM2494237 SRP100217 SRS1984871 GSM2494237 RNA-Seq TRANSCRIPTOMIC cDNA The mpkCCD cells grown on permeable inserts of 6-well transwell were washed with PBS and lysed with 100 ul Trizol solution. Total RNA was isolated using Direct-zol RNA mini prep plus (ZYMO RESEARCH, R2070) as manufacturer's protocol. Sequencing libraries were prepared using Illumina TruSeq Stranded Total RNA Sample Prep Kit (Cat#RS-122-2201) as manufacturer's instruction. Total RNA (262 ng) of each sample was applied to ribosomal RNA removal beads to deplete ribosomal RNAs. The purified RNA was fragmented and then primed with random hexamers for cDNA synthesis using reverse transcriptase and random primers. First strand cDNA and second strand cDNA were sythesized serially and ligated with indexing adapters. DNA fragments including adapters were amplified by PCR using a PCR primer cocktail. Sample libraries were qualified using Agilent Technologies Bioanalyzer. Illumina HiSeq 3000 gds 302494237 GEO Accession GSM2494237