SRX2573620 GSM2494783 SRP100163 SRS1989498 GSM2494783 RNA-Seq TRANSCRIPTOMIC cDNA Embryos were dissociated first in a Dounce homogenizer and then further using a syringe with 22G x 2" needle, followed by filtering through 20 um cell strainer and fixation in 80% methanol/PBS Captured transcriptomes were reverse transcribed while bound on barcoded oligo dT beads; PCR handles added by template switching; cDNA PCR-amplified (4 + 9 cycles); 600 pg cDNA library fragmented and amplified with Nextera XT v2 DNA sample preparation kit using custom primers for 3'-targeted amplification (Macosko et al. 2015 Cell 161) Drop-seq single-cell transcriptome profiling according to Macosko et al. 2015 Cell 161 NextSeq 500 gds 302494783 GEO Accession GSM2494783 SRX2573621 GSM2494784 SRP100163 SRS1989499 GSM2494784 RNA-Seq TRANSCRIPTOMIC cDNA Embryos were dissociated first in a Dounce homogenizer and then further using a syringe with 22G x 2" needle, followed by filtering through 20 um cell strainer and fixation in 80% methanol/PBS Captured transcriptomes were reverse transcribed while bound on barcoded oligo dT beads; PCR handles added by template switching; cDNA PCR-amplified (4 + 9 cycles); 600 pg cDNA library fragmented and amplified with Nextera XT v2 DNA sample preparation kit using custom primers for 3'-targeted amplification (Macosko et al. 2015 Cell 161) Drop-seq single-cell transcriptome profiling according to Macosko et al. 2015 Cell 161 NextSeq 500 gds 302494784 GEO Accession GSM2494784 SRX2573622 GSM2494785 SRP100163 SRS1989500 GSM2494785 RNA-Seq TRANSCRIPTOMIC cDNA Embryos were dissociated first in a Dounce homogenizer and then further using a syringe with 22G x 2" needle, followed by filtering through 20 um cell strainer and fixation in 80% methanol/PBS Captured transcriptomes were reverse transcribed while bound on barcoded oligo dT beads; PCR handles added by template switching; cDNA PCR-amplified (4 + 9 cycles); 600 pg cDNA library fragmented and amplified with Nextera XT v2 DNA sample preparation kit using custom primers for 3'-targeted amplification (Macosko et al. 2015 Cell 161) Drop-seq single-cell transcriptome profiling according to Macosko et al. 2015 Cell 161 NextSeq 500 gds 302494785 GEO Accession GSM2494785 SRX2573623 GSM2494786 SRP100163 SRS1989501 GSM2494786 RNA-Seq TRANSCRIPTOMIC cDNA Embryos were dissociated first in a Dounce homogenizer and then further using a syringe with 22G x 2" needle, followed by filtering through 20 um cell strainer and fixation in 80% methanol/PBS Captured transcriptomes were reverse transcribed while bound on barcoded oligo dT beads; PCR handles added by template switching; cDNA PCR-amplified (4 + 9 cycles); 600 pg cDNA library fragmented and amplified with Nextera XT v2 DNA sample preparation kit using custom primers for 3'-targeted amplification (Macosko et al. 2015 Cell 161) Drop-seq single-cell transcriptome profiling according to Macosko et al. 2015 Cell 161 NextSeq 500 gds 302494786 GEO Accession GSM2494786 SRX2573624 GSM2494787 SRP100163 SRS1989502 GSM2494787 RNA-Seq TRANSCRIPTOMIC cDNA Embryos were dissociated first in a Dounce homogenizer and then further using a syringe with 22G x 2" needle, followed by filtering through 20 um cell strainer and fixation in 80% methanol/PBS Captured transcriptomes were reverse transcribed while bound on barcoded oligo dT beads; PCR handles added by template switching; cDNA PCR-amplified (4 + 9 cycles); 600 pg cDNA library fragmented and amplified with Nextera XT v2 DNA sample preparation kit using custom primers for 3'-targeted amplification (Macosko et al. 2015 Cell 161) Drop-seq single-cell transcriptome profiling according to Macosko et al. 2015 Cell 161 NextSeq 500 gds 302494787 GEO Accession GSM2494787 SRX2573625 GSM2494788 SRP100163 SRS1989503 GSM2494788 RNA-Seq TRANSCRIPTOMIC cDNA Embryos were dissociated first in a Dounce homogenizer and then further using a syringe with 22G x 2" needle, followed by filtering through 20 um cell strainer and fixation in 80% methanol/PBS Captured transcriptomes were reverse transcribed while bound on barcoded oligo dT beads; PCR handles added by template switching; cDNA PCR-amplified (4 + 9 cycles); 600 pg cDNA library fragmented and amplified with Nextera XT v2 DNA sample preparation kit using custom primers for 3'-targeted amplification (Macosko et al. 2015 Cell 161) Drop-seq single-cell transcriptome profiling according to Macosko et al. 2015 Cell 161 NextSeq 500 gds 302494788 GEO Accession GSM2494788 SRX2573626 GSM2494789 SRP100163 SRS1989504 GSM2494789 RNA-Seq TRANSCRIPTOMIC cDNA Embryos were dissociated first in a Dounce homogenizer and then further using a syringe with 22G x 2" needle, followed by filtering through 20 um cell strainer and fixation in 80% methanol/PBS Captured transcriptomes were reverse transcribed while bound on barcoded oligo dT beads; PCR handles added by template switching; cDNA PCR-amplified (4 + 9 cycles); 600 pg cDNA library fragmented and amplified with Nextera XT v2 DNA sample preparation kit using custom primers for 3'-targeted amplification (Macosko et al. 2015 Cell 161) Drop-seq single-cell transcriptome profiling according to Macosko et al. 2015 Cell 161 NextSeq 500 gds 302494789 GEO Accession GSM2494789 SRX2573627 GSM2494790 SRP100163 SRS1989505 GSM2494790 RNA-Seq TRANSCRIPTOMIC cDNA Whole, live embryos were dissolved in TRIZOL, shock-frozen in liquid nitrogen, then total RNA was extracted Libraries were generated from 200 ng RNA with NEBNext Ultra RNA Library Prep Kit for Illumina using poly(A) mRNA magnetic isolation. mRNAseq, unstranded NextSeq 500 gds 302494790 GEO Accession GSM2494790 SRX2573628 GSM2494791 SRP100163 SRS1989506 GSM2494791 RNA-Seq TRANSCRIPTOMIC cDNA Whole, live embryos were dissolved in TRIZOL, shock-frozen in liquid nitrogen, then total RNA was extracted Libraries were generated from 200 ng RNA with NEBNext Ultra RNA Library Prep Kit for Illumina using poly(A) mRNA magnetic isolation. mRNAseq, unstranded NextSeq 500 gds 302494791 GEO Accession GSM2494791