SRP100199 PRJNA359099 oral metagenome Targeted loci This study aimed to evaluate the bacterial diversity in occlusal biofilms, and its relationship with clinical surface diagnosis and dietary habits. Anamneses were recorded from thirteen 12-year-old children. Biofilm samples collected from occlusal surfaces of 46 permanent second molars were analyzed by 16S rRNA amplicon sequencing. oral metagenome