SRX2584627 LID_SC_USC_1553 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998391 SC_USC_1553 LID_SC_USC_1553 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584628 LID_SC_USC_1554 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998390 SC_USC_1554 LID_SC_USC_1554 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584629 LID_SC_USC_1555 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998393 SC_USC_1555 LID_SC_USC_1555 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584630 LID_SC_USC_1556 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998392 SC_USC_1556 LID_SC_USC_1556 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584631 LID_SC_USC_1557 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998394 SC_USC_1557 LID_SC_USC_1557 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584632 LID_SC_USC_1558 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998396 SC_USC_1558 LID_SC_USC_1558 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584633 LID_SC_USC_1559 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998395 SC_USC_1559 LID_SC_USC_1559 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584634 LID_SC_USC_1560 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998397 SC_USC_1560 LID_SC_USC_1560 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584635 LID_SC_USC_1561 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998400 SC_USC_1561 LID_SC_USC_1561 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584636 LID_SC_USC_1562 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998398 SC_USC_1562 LID_SC_USC_1562 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584637 LID_SC_USC_1563 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998399 SC_USC_1563 LID_SC_USC_1563 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584638 LID_SC_USC_1564 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998401 SC_USC_1564 LID_SC_USC_1564 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584639 LID_SC_USC_1565 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998402 SC_USC_1565 LID_SC_USC_1565 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584640 LID_SC_USC_1566 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998403 SC_USC_1566 LID_SC_USC_1566 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584641 LID_SC_USC_1567 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998404 SC_USC_1567 LID_SC_USC_1567 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584642 LID_SC_USC_1568 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998405 SC_USC_1568 LID_SC_USC_1568 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584643 LID_SC_USC_1569 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998407 SC_USC_1569 LID_SC_USC_1569 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584644 LID_SC_USC_1570 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998406 SC_USC_1570 LID_SC_USC_1570 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584645 LID_SC_USC_1571 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998408 SC_USC_1571 LID_SC_USC_1571 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584646 LID_SC_USC_1572 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998409 SC_USC_1572 LID_SC_USC_1572 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584647 LID_SC_USC_1573 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998410 SC_USC_1573 LID_SC_USC_1573 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584648 LID_SC_USC_1574 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998411 SC_USC_1574 LID_SC_USC_1574 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584649 LID_SC_USC_1575 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998412 SC_USC_1575 LID_SC_USC_1575 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584650 LID_SC_USC_1576 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998414 SC_USC_1576 LID_SC_USC_1576 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584651 LID_SC_USC_1577 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998413 SC_USC_1577 LID_SC_USC_1577 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584652 LID_SC_USC_1578 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998415 SC_USC_1578 LID_SC_USC_1578 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584653 LID_SC_USC_1579 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998416 SC_USC_1579 LID_SC_USC_1579 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584654 LID_SC_USC_1580 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998417 SC_USC_1580 LID_SC_USC_1580 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584655 LID_SC_USC_1581 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998418 SC_USC_1581 LID_SC_USC_1581 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584656 LID_SC_USC_1582 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998419 SC_USC_1582 LID_SC_USC_1582 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584657 LID_SC_USC_1583 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998420 SC_USC_1583 LID_SC_USC_1583 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584658 LID_SC_USC_1584 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998421 SC_USC_1584 LID_SC_USC_1584 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584659 LID_SC_USC_1585 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998422 SC_USC_1585 LID_SC_USC_1585 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584660 LID_SC_USC_1586 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998423 SC_USC_1586 LID_SC_USC_1586 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584661 LID_SC_USC_1587 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998424 SC_USC_1587 LID_SC_USC_1587 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584662 LID_SC_USC_1588 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998426 SC_USC_1588 LID_SC_USC_1588 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584663 LID_SC_USC_1589 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998425 SC_USC_1589 LID_SC_USC_1589 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584664 LID_SC_USC_1590 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998428 SC_USC_1590 LID_SC_USC_1590 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584665 LID_SC_USC_1591 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998429 SC_USC_1591 LID_SC_USC_1591 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584666 LID_SC_USC_1592 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998427 SC_USC_1592 LID_SC_USC_1592 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584667 LID_SC_USC_1593 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998431 SC_USC_1593 LID_SC_USC_1593 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584668 LID_SC_USC_1594 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998430 SC_USC_1594 LID_SC_USC_1594 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584669 LID_SC_USC_1595 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998432 SC_USC_1595 LID_SC_USC_1595 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584670 LID_SC_USC_1596 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998437 SC_USC_1596 LID_SC_USC_1596 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584671 LID_SC_USC_1597 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998433 SC_USC_1597 LID_SC_USC_1597 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584672 LID_SC_USC_1598 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998434 SC_USC_1598 LID_SC_USC_1598 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584673 LID_SC_USC_1599 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998435 SC_USC_1599 LID_SC_USC_1599 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584674 LID_SC_USC_1600 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998436 SC_USC_1600 LID_SC_USC_1600 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584675 LID_SC_USC_1601 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998438 SC_USC_1601 LID_SC_USC_1601 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584676 LID_SC_USC_1602 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998439 SC_USC_1602 LID_SC_USC_1602 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584677 LID_SC_USC_1603 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998440 SC_USC_1603 LID_SC_USC_1603 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584678 LID_SC_USC_1604 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998441 SC_USC_1604 LID_SC_USC_1604 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584679 LID_SC_USC_1605 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998442 SC_USC_1605 LID_SC_USC_1605 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584680 LID_SC_USC_1606 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998443 SC_USC_1606 LID_SC_USC_1606 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584681 LID_SC_USC_1607 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998444 SC_USC_1607 LID_SC_USC_1607 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584682 LID_SC_USC_1608 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998453 SC_USC_1608 LID_SC_USC_1608 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584683 LID_SC_USC_1609 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998445 SC_USC_1609 LID_SC_USC_1609 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584684 LID_SC_USC_1610 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998446 SC_USC_1610 LID_SC_USC_1610 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584685 LID_SC_USC_1611 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998447 SC_USC_1611 LID_SC_USC_1611 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584686 LID_SC_USC_1612 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998448 SC_USC_1612 LID_SC_USC_1612 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584687 LID_SC_USC_1613 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998449 SC_USC_1613 LID_SC_USC_1613 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584688 LID_SC_USC_1614 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998450 SC_USC_1614 LID_SC_USC_1614 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584689 LID_SC_USC_1615 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998451 SC_USC_1615 LID_SC_USC_1615 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584690 LID_SC_USC_1616 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998452 SC_USC_1616 LID_SC_USC_1616 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584691 LID_SC_USC_1617 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998454 SC_USC_1617 LID_SC_USC_1617 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584692 LID_SC_USC_1618 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998455 SC_USC_1618 LID_SC_USC_1618 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584693 LID_SC_USC_1619 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998456 SC_USC_1619 LID_SC_USC_1619 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584694 LID_SC_USC_1620 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998457 SC_USC_1620 LID_SC_USC_1620 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584695 LID_SC_USC_1621 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998458 SC_USC_1621 LID_SC_USC_1621 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584696 LID_SC_USC_1622 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998459 SC_USC_1622 LID_SC_USC_1622 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584697 LID_SC_USC_1623 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998462 SC_USC_1623 LID_SC_USC_1623 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584698 LID_SC_USC_1624 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998460 SC_USC_1624 LID_SC_USC_1624 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584699 LID_SC_USC_1625 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998461 SC_USC_1625 LID_SC_USC_1625 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584700 LID_SC_USC_1626 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998463 SC_USC_1626 LID_SC_USC_1626 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584701 LID_SC_USC_1627 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998464 SC_USC_1627 LID_SC_USC_1627 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584702 LID_SC_USC_1628 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998465 SC_USC_1628 LID_SC_USC_1628 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584703 LID_SC_USC_1629 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998466 SC_USC_1629 LID_SC_USC_1629 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584704 LID_SC_USC_1630 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998467 SC_USC_1630 LID_SC_USC_1630 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584705 LID_SC_USC_1631 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998471 SC_USC_1631 LID_SC_USC_1631 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584706 LID_SC_USC_1632 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998468 SC_USC_1632 LID_SC_USC_1632 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584707 LID_SC_USC_1633 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998469 SC_USC_1633 LID_SC_USC_1633 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584708 LID_SC_USC_1634 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998470 SC_USC_1634 LID_SC_USC_1634 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584709 LID_SC_USC_1635 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998472 SC_USC_1635 LID_SC_USC_1635 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584710 LID_SC_USC_1636 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998473 SC_USC_1636 LID_SC_USC_1636 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584711 LID_SC_USC_1637 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998474 SC_USC_1637 LID_SC_USC_1637 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584712 LID_SC_USC_1638 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998475 SC_USC_1638 LID_SC_USC_1638 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584713 LID_SC_USC_1639 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998476 SC_USC_1639 LID_SC_USC_1639 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584714 LID_SC_USC_1640 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998477 SC_USC_1640 LID_SC_USC_1640 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584715 LID_SC_USC_1641 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998478 SC_USC_1641 LID_SC_USC_1641 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584716 LID_SC_USC_1642 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998479 SC_USC_1642 LID_SC_USC_1642 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584717 LID_SC_USC_1643 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998480 SC_USC_1643 LID_SC_USC_1643 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584718 LID_SC_USC_1644 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998481 SC_USC_1644 LID_SC_USC_1644 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584719 LID_SC_USC_1645 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998483 SC_USC_1645 LID_SC_USC_1645 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584720 LID_SC_USC_1646 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998484 SC_USC_1646 LID_SC_USC_1646 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584721 LID_SC_USC_1647 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998482 SC_USC_1647 LID_SC_USC_1647 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584722 LID_SC_USC_1648 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998485 SC_USC_1648 LID_SC_USC_1648 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584723 LID_SC_USC_1649 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998487 SC_USC_1649 LID_SC_USC_1649 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584724 LID_SC_USC_1650 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998486 SC_USC_1650 LID_SC_USC_1650 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584725 LID_SC_USC_1651 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998488 SC_USC_1651 LID_SC_USC_1651 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584726 LID_SC_USC_1652 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998489 SC_USC_1652 LID_SC_USC_1652 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584727 LID_SC_USC_1653 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998490 SC_USC_1653 LID_SC_USC_1653 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584728 LID_SC_USC_1654 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998491 SC_USC_1654 LID_SC_USC_1654 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584729 LID_SC_USC_1655 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998492 SC_USC_1655 LID_SC_USC_1655 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584730 LID_SC_USC_1656 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998493 SC_USC_1656 LID_SC_USC_1656 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584731 LID_SC_USC_1657 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998494 SC_USC_1657 LID_SC_USC_1657 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584732 LID_SC_USC_1658 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998495 SC_USC_1658 LID_SC_USC_1658 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584733 LID_SC_USC_1659 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998496 SC_USC_1659 LID_SC_USC_1659 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584734 LID_SC_USC_1660 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998497 SC_USC_1660 LID_SC_USC_1660 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584735 LID_SC_USC_1661 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998498 SC_USC_1661 LID_SC_USC_1661 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584736 LID_SC_USC_1662 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998499 SC_USC_1662 LID_SC_USC_1662 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584737 LID_SC_USC_1663 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998501 SC_USC_1663 LID_SC_USC_1663 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584738 LID_SC_USC_1664 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998500 SC_USC_1664 LID_SC_USC_1664 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584739 LID_SC_USC_1665 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998502 SC_USC_1665 LID_SC_USC_1665 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584740 LID_SC_USC_1666 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998503 SC_USC_1666 LID_SC_USC_1666 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584741 LID_SC_USC_1667 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998505 SC_USC_1667 LID_SC_USC_1667 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584742 LID_SC_USC_1668 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998504 SC_USC_1668 LID_SC_USC_1668 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584743 LID_SC_USC_1669 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998506 SC_USC_1669 LID_SC_USC_1669 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584744 LID_SC_USC_1670 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998507 SC_USC_1670 LID_SC_USC_1670 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584745 LID_SC_USC_1671 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998508 SC_USC_1671 LID_SC_USC_1671 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584746 LID_SC_USC_1672 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998509 SC_USC_1672 LID_SC_USC_1672 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584747 LID_SC_USC_1673 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998510 SC_USC_1673 LID_SC_USC_1673 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584748 LID_SC_USC_1674 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998511 SC_USC_1674 LID_SC_USC_1674 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584749 LID_SC_USC_1675 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998512 SC_USC_1675 LID_SC_USC_1675 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584750 LID_SC_USC_1676 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998513 SC_USC_1676 LID_SC_USC_1676 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584751 LID_SC_USC_1677 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998514 SC_USC_1677 LID_SC_USC_1677 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584752 LID_SC_USC_1678 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998515 SC_USC_1678 LID_SC_USC_1678 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584753 LID_SC_USC_1679 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998516 SC_USC_1679 LID_SC_USC_1679 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584754 LID_SC_USC_1680 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998517 SC_USC_1680 LID_SC_USC_1680 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584755 LID_SC_USC_1681 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998519 SC_USC_1681 LID_SC_USC_1681 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584756 LID_SC_USC_1682 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998518 SC_USC_1682 LID_SC_USC_1682 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584757 LID_SC_USC_1683 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998520 SC_USC_1683 LID_SC_USC_1683 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584758 LID_SC_USC_1684 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998521 SC_USC_1684 LID_SC_USC_1684 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584759 LID_SC_USC_1685 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998523 SC_USC_1685 LID_SC_USC_1685 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584760 LID_SC_USC_1686 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998524 SC_USC_1686 LID_SC_USC_1686 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584761 LID_SC_USC_1687 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998522 SC_USC_1687 LID_SC_USC_1687 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584762 LID_SC_USC_1688 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998525 SC_USC_1688 LID_SC_USC_1688 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584763 LID_SC_USC_1689 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998527 SC_USC_1689 LID_SC_USC_1689 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584764 LID_SC_USC_1690 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998526 SC_USC_1690 LID_SC_USC_1690 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584765 LID_SC_USC_1691 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998528 SC_USC_1691 LID_SC_USC_1691 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584766 LID_SC_USC_1692 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998532 SC_USC_1692 LID_SC_USC_1692 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584767 LID_SC_USC_1693 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998529 SC_USC_1693 LID_SC_USC_1693 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584768 LID_SC_USC_1694 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998531 SC_USC_1694 LID_SC_USC_1694 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584769 LID_SC_USC_1695 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998530 SC_USC_1695 LID_SC_USC_1695 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584770 LID_SC_USC_1696 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998533 SC_USC_1696 LID_SC_USC_1696 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584771 LID_SC_USC_1697 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998535 SC_USC_1697 LID_SC_USC_1697 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584772 LID_SC_USC_1698 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998534 SC_USC_1698 LID_SC_USC_1698 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584773 LID_SC_USC_1699 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998536 SC_USC_1699 LID_SC_USC_1699 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584774 LID_SC_USC_1700 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998537 SC_USC_1700 LID_SC_USC_1700 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584775 LID_SC_USC_1701 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998538 SC_USC_1701 LID_SC_USC_1701 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584776 LID_SC_USC_1702 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998539 SC_USC_1702 LID_SC_USC_1702 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584777 LID_SC_USC_1703 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998540 SC_USC_1703 LID_SC_USC_1703 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584778 LID_SC_USC_1704 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998541 SC_USC_1704 LID_SC_USC_1704 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584779 LID_SC_USC_1705 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998542 SC_USC_1705 LID_SC_USC_1705 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584780 LID_SC_USC_1706 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998545 SC_USC_1706 LID_SC_USC_1706 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584781 LID_SC_USC_1707 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998544 SC_USC_1707 LID_SC_USC_1707 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584782 LID_SC_USC_1708 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998543 SC_USC_1708 LID_SC_USC_1708 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584783 LID_SC_USC_1709 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998546 SC_USC_1709 LID_SC_USC_1709 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584784 LID_SC_USC_1710 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998547 SC_USC_1710 LID_SC_USC_1710 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584785 LID_SC_USC_1711 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998548 SC_USC_1711 LID_SC_USC_1711 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584786 LID_SC_USC_1712 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998549 SC_USC_1712 LID_SC_USC_1712 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584787 LID_SC_USC_1713 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998550 SC_USC_1713 LID_SC_USC_1713 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584788 LID_SC_USC_1714 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998551 SC_USC_1714 LID_SC_USC_1714 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584789 LID_SC_USC_1715 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998552 SC_USC_1715 LID_SC_USC_1715 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584790 LID_SC_USC_1716 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998555 SC_USC_1716 LID_SC_USC_1716 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584791 LID_SC_USC_1717 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998554 SC_USC_1717 LID_SC_USC_1717 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584792 LID_SC_USC_1718 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998553 SC_USC_1718 LID_SC_USC_1718 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584793 LID_SC_USC_1719 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998556 SC_USC_1719 LID_SC_USC_1719 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584794 LID_SC_USC_1720 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998557 SC_USC_1720 LID_SC_USC_1720 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584795 LID_SC_USC_1721 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998559 SC_USC_1721 LID_SC_USC_1721 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584796 LID_SC_USC_1722 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998558 SC_USC_1722 LID_SC_USC_1722 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584797 LID_SC_USC_1723 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998560 SC_USC_1723 LID_SC_USC_1723 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584798 LID_SC_USC_1724 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998561 SC_USC_1724 LID_SC_USC_1724 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584799 LID_SC_USC_1725 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998562 SC_USC_1725 LID_SC_USC_1725 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584800 LID_SC_USC_1726 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998563 SC_USC_1726 LID_SC_USC_1726 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584801 LID_SC_USC_1727 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998564 SC_USC_1727 LID_SC_USC_1727 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584802 LID_SC_USC_1728 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998565 SC_USC_1728 LID_SC_USC_1728 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584803 LID_SC_USC_1729 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998566 SC_USC_1729 LID_SC_USC_1729 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584804 LID_SC_USC_1730 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998568 SC_USC_1730 LID_SC_USC_1730 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584805 LID_SC_USC_1731 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998567 SC_USC_1731 LID_SC_USC_1731 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584806 LID_SC_USC_1732 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998569 SC_USC_1732 LID_SC_USC_1732 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584807 LID_SC_USC_1733 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998570 SC_USC_1733 LID_SC_USC_1733 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584808 LID_SC_USC_1734 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998571 SC_USC_1734 LID_SC_USC_1734 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584809 LID_SC_USC_1735 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998572 SC_USC_1735 LID_SC_USC_1735 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584810 LID_SC_USC_1736 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998573 SC_USC_1736 LID_SC_USC_1736 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584811 LID_SC_USC_1737 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998575 SC_USC_1737 LID_SC_USC_1737 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584812 LID_SC_USC_1738 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998574 SC_USC_1738 LID_SC_USC_1738 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584813 LID_SC_USC_1739 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998578 SC_USC_1739 LID_SC_USC_1739 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584814 LID_SC_USC_1740 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998577 SC_USC_1740 LID_SC_USC_1740 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584815 LID_SC_USC_1741 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998576 SC_USC_1741 LID_SC_USC_1741 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584816 LID_SC_USC_1742 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998579 SC_USC_1742 LID_SC_USC_1742 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584817 LID_SC_USC_1743 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998580 SC_USC_1743 LID_SC_USC_1743 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584818 LID_SC_USC_1744 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998581 SC_USC_1744 LID_SC_USC_1744 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584819 LID_SC_USC_1745 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998583 SC_USC_1745 LID_SC_USC_1745 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584820 LID_SC_USC_1746 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998582 SC_USC_1746 LID_SC_USC_1746 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584821 LID_SC_USC_1747 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998584 SC_USC_1747 LID_SC_USC_1747 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584822 LID_SC_USC_1748 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998585 SC_USC_1748 LID_SC_USC_1748 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584823 LID_SC_USC_1749 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998586 SC_USC_1749 LID_SC_USC_1749 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584824 LID_SC_USC_1750 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998587 SC_USC_1750 LID_SC_USC_1750 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584825 LID_SC_USC_1751 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998588 SC_USC_1751 LID_SC_USC_1751 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584826 LID_SC_USC_1752 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998589 SC_USC_1752 LID_SC_USC_1752 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584827 LID_SC_USC_1753 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998590 SC_USC_1753 LID_SC_USC_1753 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584828 LID_SC_USC_1754 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998591 SC_USC_1754 LID_SC_USC_1754 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584829 LID_SC_USC_1755 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998592 SC_USC_1755 LID_SC_USC_1755 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2000 alignment_software STAR SRX2584830 LID_SC_USC_1756 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998598 SC_USC_1756 LID_SC_USC_1756 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584831 LID_SC_USC_1757 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998594 SC_USC_1757 LID_SC_USC_1757 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584832 LID_SC_USC_1758 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998593 SC_USC_1758 LID_SC_USC_1758 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584833 LID_SC_USC_1759 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998595 SC_USC_1759 LID_SC_USC_1759 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584834 LID_SC_USC_1760 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998597 SC_USC_1760 LID_SC_USC_1760 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584835 LID_SC_USC_1761 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998596 SC_USC_1761 LID_SC_USC_1761 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584836 LID_SC_USC_1762 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998599 SC_USC_1762 LID_SC_USC_1762 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584837 LID_SC_USC_1763 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998600 SC_USC_1763 LID_SC_USC_1763 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584838 LID_SC_USC_1764 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998601 SC_USC_1764 LID_SC_USC_1764 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584839 LID_SC_USC_1765 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998602 SC_USC_1765 LID_SC_USC_1765 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584840 LID_SC_USC_1766 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998603 SC_USC_1766 LID_SC_USC_1766 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584841 LID_SC_USC_1767 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998604 SC_USC_1767 LID_SC_USC_1767 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584842 LID_SC_USC_1768 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998605 SC_USC_1768 LID_SC_USC_1768 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584843 LID_SC_USC_1769 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998606 SC_USC_1769 LID_SC_USC_1769 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584844 LID_SC_USC_1770 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998607 SC_USC_1770 LID_SC_USC_1770 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584845 LID_SC_USC_1771 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998608 SC_USC_1771 LID_SC_USC_1771 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584846 LID_SC_USC_1772 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998611 SC_USC_1772 LID_SC_USC_1772 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584847 LID_SC_USC_1773 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998609 SC_USC_1773 LID_SC_USC_1773 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584848 LID_SC_USC_1774 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998610 SC_USC_1774 LID_SC_USC_1774 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584849 LID_SC_USC_1775 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998612 SC_USC_1775 LID_SC_USC_1775 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584850 LID_SC_USC_1776 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998614 SC_USC_1776 LID_SC_USC_1776 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584851 LID_SC_USC_1777 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998613 SC_USC_1777 LID_SC_USC_1777 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584852 LID_SC_USC_1778 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998615 SC_USC_1778 LID_SC_USC_1778 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584853 LID_SC_USC_1779 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998616 SC_USC_1779 LID_SC_USC_1779 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584854 LID_SC_USC_1780 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998617 SC_USC_1780 LID_SC_USC_1780 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584855 LID_SC_USC_1781 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998618 SC_USC_1781 LID_SC_USC_1781 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584856 LID_SC_USC_1782 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998619 SC_USC_1782 LID_SC_USC_1782 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584857 LID_SC_USC_1783 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998621 SC_USC_1783 LID_SC_USC_1783 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584858 LID_SC_USC_1784 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998620 SC_USC_1784 LID_SC_USC_1784 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584859 LID_SC_USC_1785 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998622 SC_USC_1785 LID_SC_USC_1785 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584860 LID_SC_USC_1786 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998623 SC_USC_1786 LID_SC_USC_1786 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584861 LID_SC_USC_1787 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998624 SC_USC_1787 LID_SC_USC_1787 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584862 LID_SC_USC_1788 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998625 SC_USC_1788 LID_SC_USC_1788 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584863 LID_SC_USC_1789 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998626 SC_USC_1789 LID_SC_USC_1789 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584864 LID_SC_USC_1790 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998627 SC_USC_1790 LID_SC_USC_1790 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584865 LID_SC_USC_1791 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998628 SC_USC_1791 LID_SC_USC_1791 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584866 LID_SC_USC_1792 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998629 SC_USC_1792 LID_SC_USC_1792 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584867 LID_SC_USC_1793 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998630 SC_USC_1793 LID_SC_USC_1793 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584868 LID_SC_USC_1794 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998631 SC_USC_1794 LID_SC_USC_1794 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584869 LID_SC_USC_1795 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998633 SC_USC_1795 LID_SC_USC_1795 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584870 LID_SC_USC_1796 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998632 SC_USC_1796 LID_SC_USC_1796 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584871 LID_SC_USC_1797 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998634 SC_USC_1797 LID_SC_USC_1797 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584872 LID_SC_USC_1798 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998636 SC_USC_1798 LID_SC_USC_1798 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584873 LID_SC_USC_1799 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998638 SC_USC_1799 LID_SC_USC_1799 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584874 LID_SC_USC_1800 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998637 SC_USC_1800 LID_SC_USC_1800 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584875 LID_SC_USC_1801 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998635 SC_USC_1801 LID_SC_USC_1801 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584876 LID_SC_USC_1802 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998639 SC_USC_1802 LID_SC_USC_1802 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584877 LID_SC_USC_1803 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998640 SC_USC_1803 LID_SC_USC_1803 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584878 LID_SC_USC_1804 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998641 SC_USC_1804 LID_SC_USC_1804 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584879 LID_SC_USC_1805 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998642 SC_USC_1805 LID_SC_USC_1805 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584880 LID_SC_USC_1806 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998643 SC_USC_1806 LID_SC_USC_1806 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584881 LID_SC_USC_1807 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998644 SC_USC_1807 LID_SC_USC_1807 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584882 LID_SC_USC_1808 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998645 SC_USC_1808 LID_SC_USC_1808 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584883 LID_SC_USC_1809 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998646 SC_USC_1809 LID_SC_USC_1809 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584884 LID_SC_USC_1810 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998647 SC_USC_1810 LID_SC_USC_1810 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584885 LID_SC_USC_1811 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998649 SC_USC_1811 LID_SC_USC_1811 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584886 LID_SC_USC_1812 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998648 SC_USC_1812 LID_SC_USC_1812 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584887 LID_SC_USC_1813 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998651 SC_USC_1813 LID_SC_USC_1813 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584888 LID_SC_USC_1814 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998650 SC_USC_1814 LID_SC_USC_1814 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584889 LID_SC_USC_1815 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998652 SC_USC_1815 LID_SC_USC_1815 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584890 LID_SC_USC_1816 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998653 SC_USC_1816 LID_SC_USC_1816 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584891 LID_SC_USC_1817 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998654 SC_USC_1817 LID_SC_USC_1817 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584892 LID_SC_USC_1818 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998655 SC_USC_1818 LID_SC_USC_1818 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584893 LID_SC_USC_1819 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998656 SC_USC_1819 LID_SC_USC_1819 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584894 LID_SC_USC_1820 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998657 SC_USC_1820 LID_SC_USC_1820 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584895 LID_SC_USC_1821 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998658 SC_USC_1821 LID_SC_USC_1821 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584896 LID_SC_USC_1822 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998659 SC_USC_1822 LID_SC_USC_1822 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584897 LID_SC_USC_1823 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998660 SC_USC_1823 LID_SC_USC_1823 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584898 LID_SC_USC_1824 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998661 SC_USC_1824 LID_SC_USC_1824 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584899 LID_SC_USC_1825 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998662 SC_USC_1825 LID_SC_USC_1825 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584900 LID_SC_USC_1826 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998663 SC_USC_1826 LID_SC_USC_1826 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584901 LID_SC_USC_1827 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998664 SC_USC_1827 LID_SC_USC_1827 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584902 LID_SC_USC_1828 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998665 SC_USC_1828 LID_SC_USC_1828 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584903 LID_SC_USC_1829 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998666 SC_USC_1829 LID_SC_USC_1829 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584904 LID_SC_USC_1830 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998667 SC_USC_1830 LID_SC_USC_1830 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584905 LID_SC_USC_1831 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998668 SC_USC_1831 LID_SC_USC_1831 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584906 LID_SC_USC_1832 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998669 SC_USC_1832 LID_SC_USC_1832 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584907 LID_SC_USC_1833 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998670 SC_USC_1833 LID_SC_USC_1833 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584908 LID_SC_USC_1834 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998671 SC_USC_1834 LID_SC_USC_1834 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584909 LID_SC_USC_1835 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998672 SC_USC_1835 LID_SC_USC_1835 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584910 LID_SC_USC_1836 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998673 SC_USC_1836 LID_SC_USC_1836 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584911 LID_SC_USC_1837 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998674 SC_USC_1837 LID_SC_USC_1837 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584912 LID_SC_USC_1838 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998675 SC_USC_1838 LID_SC_USC_1838 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584913 LID_SC_USC_1839 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998677 SC_USC_1839 LID_SC_USC_1839 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584914 LID_SC_USC_1840 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998676 SC_USC_1840 LID_SC_USC_1840 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584915 LID_SC_USC_1841 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998678 SC_USC_1841 LID_SC_USC_1841 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584916 LID_SC_USC_1842 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998679 SC_USC_1842 LID_SC_USC_1842 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584917 LID_SC_USC_1843 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998680 SC_USC_1843 LID_SC_USC_1843 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584918 LID_SC_USC_1844 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998681 SC_USC_1844 LID_SC_USC_1844 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584919 LID_SC_USC_1845 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998682 SC_USC_1845 LID_SC_USC_1845 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584920 LID_SC_USC_1846 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998684 SC_USC_1846 LID_SC_USC_1846 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584921 LID_SC_USC_1847 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998683 SC_USC_1847 LID_SC_USC_1847 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584922 LID_SC_USC_1848 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998685 SC_USC_1848 LID_SC_USC_1848 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584923 LID_SC_USC_1849 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998686 SC_USC_1849 LID_SC_USC_1849 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584924 LID_SC_USC_1850 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998687 SC_USC_1850 LID_SC_USC_1850 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584925 LID_SC_USC_1851 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998689 SC_USC_1851 LID_SC_USC_1851 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584926 LID_SC_USC_1852 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998688 SC_USC_1852 LID_SC_USC_1852 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584927 LID_SC_USC_1853 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998690 SC_USC_1853 LID_SC_USC_1853 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584928 LID_SC_USC_1854 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998691 SC_USC_1854 LID_SC_USC_1854 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584929 LID_SC_USC_1855 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998692 SC_USC_1855 LID_SC_USC_1855 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584930 LID_SC_USC_1856 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998693 SC_USC_1856 LID_SC_USC_1856 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584931 LID_SC_USC_1857 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998694 SC_USC_1857 LID_SC_USC_1857 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584932 LID_SC_USC_1858 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998695 SC_USC_1858 LID_SC_USC_1858 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584933 LID_SC_USC_1859 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998696 SC_USC_1859 LID_SC_USC_1859 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584934 LID_SC_USC_1860 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998697 SC_USC_1860 LID_SC_USC_1860 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584935 LID_SC_USC_1861 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998698 SC_USC_1861 LID_SC_USC_1861 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584936 LID_SC_USC_1862 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998699 SC_USC_1862 LID_SC_USC_1862 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584937 LID_SC_USC_1863 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998700 SC_USC_1863 LID_SC_USC_1863 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584938 LID_SC_USC_1864 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998701 SC_USC_1864 LID_SC_USC_1864 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584939 LID_SC_USC_1865 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998704 SC_USC_1865 LID_SC_USC_1865 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584940 LID_SC_USC_1866 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998702 SC_USC_1866 LID_SC_USC_1866 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584941 LID_SC_USC_1867 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998703 SC_USC_1867 LID_SC_USC_1867 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584942 LID_SC_USC_1868 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998705 SC_USC_1868 LID_SC_USC_1868 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584943 LID_SC_USC_1869 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998706 SC_USC_1869 LID_SC_USC_1869 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584944 LID_SC_USC_1870 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998707 SC_USC_1870 LID_SC_USC_1870 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584945 LID_SC_USC_1871 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998708 SC_USC_1871 LID_SC_USC_1871 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584946 LID_SC_USC_1872 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998709 SC_USC_1872 LID_SC_USC_1872 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584947 LID_SC_USC_1873 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998710 SC_USC_1873 LID_SC_USC_1873 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584948 LID_SC_USC_1874 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998711 SC_USC_1874 LID_SC_USC_1874 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584949 LID_SC_USC_1875 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998712 SC_USC_1875 LID_SC_USC_1875 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584950 LID_SC_USC_1876 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998713 SC_USC_1876 LID_SC_USC_1876 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584951 LID_SC_USC_1877 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998714 SC_USC_1877 LID_SC_USC_1877 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584952 LID_SC_USC_1878 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998715 SC_USC_1878 LID_SC_USC_1878 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584953 LID_SC_USC_1879 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998716 SC_USC_1879 LID_SC_USC_1879 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584954 LID_SC_USC_1880 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998717 SC_USC_1880 LID_SC_USC_1880 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584955 LID_SC_USC_1881 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998720 SC_USC_1881 LID_SC_USC_1881 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584956 LID_SC_USC_1882 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998718 SC_USC_1882 LID_SC_USC_1882 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584957 LID_SC_USC_1883 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998719 SC_USC_1883 LID_SC_USC_1883 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584958 LID_SC_USC_1884 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998721 SC_USC_1884 LID_SC_USC_1884 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584959 LID_SC_USC_1885 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998722 SC_USC_1885 LID_SC_USC_1885 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584960 LID_SC_USC_1886 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998723 SC_USC_1886 LID_SC_USC_1886 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584961 LID_SC_USC_1887 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998724 SC_USC_1887 LID_SC_USC_1887 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584962 LID_SC_USC_1888 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998728 SC_USC_1888 LID_SC_USC_1888 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584963 LID_SC_USC_1889 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998725 SC_USC_1889 LID_SC_USC_1889 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584964 LID_SC_USC_1890 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998727 SC_USC_1890 LID_SC_USC_1890 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584965 LID_SC_USC_1891 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998726 SC_USC_1891 LID_SC_USC_1891 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584966 LID_SC_USC_1892 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998729 SC_USC_1892 LID_SC_USC_1892 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584967 LID_SC_USC_1893 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998730 SC_USC_1893 LID_SC_USC_1893 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584968 LID_SC_USC_1894 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998731 SC_USC_1894 LID_SC_USC_1894 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584969 LID_SC_USC_1895 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998732 SC_USC_1895 LID_SC_USC_1895 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584970 LID_SC_USC_1896 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998733 SC_USC_1896 LID_SC_USC_1896 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584971 LID_SC_USC_1897 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998734 SC_USC_1897 LID_SC_USC_1897 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584972 LID_SC_USC_1898 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998735 SC_USC_1898 LID_SC_USC_1898 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584973 LID_SC_USC_1899 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998736 SC_USC_1899 LID_SC_USC_1899 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584974 LID_SC_USC_1900 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998737 SC_USC_1900 LID_SC_USC_1900 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584975 LID_SC_USC_1901 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998738 SC_USC_1901 LID_SC_USC_1901 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584976 LID_SC_USC_1902 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998739 SC_USC_1902 LID_SC_USC_1902 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584977 LID_SC_USC_1903 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998742 SC_USC_1903 LID_SC_USC_1903 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584978 LID_SC_USC_1904 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998740 SC_USC_1904 LID_SC_USC_1904 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584979 LID_SC_USC_1905 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998741 SC_USC_1905 LID_SC_USC_1905 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584980 LID_SC_USC_1906 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998743 SC_USC_1906 LID_SC_USC_1906 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584981 LID_SC_USC_1907 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998745 SC_USC_1907 LID_SC_USC_1907 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584982 LID_SC_USC_1908 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998744 SC_USC_1908 LID_SC_USC_1908 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584983 LID_SC_USC_1909 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998746 SC_USC_1909 LID_SC_USC_1909 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584984 LID_SC_USC_1910 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998747 SC_USC_1910 LID_SC_USC_1910 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584985 LID_SC_USC_1911 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998748 SC_USC_1911 LID_SC_USC_1911 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584986 LID_SC_USC_1912 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998751 SC_USC_1912 LID_SC_USC_1912 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584987 LID_SC_USC_1913 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998749 SC_USC_1913 LID_SC_USC_1913 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584988 LID_SC_USC_1914 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998750 SC_USC_1914 LID_SC_USC_1914 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584989 LID_SC_USC_1915 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998753 SC_USC_1915 LID_SC_USC_1915 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584990 LID_SC_USC_1916 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998752 SC_USC_1916 LID_SC_USC_1916 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584991 LID_SC_USC_1917 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998754 SC_USC_1917 LID_SC_USC_1917 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584992 LID_SC_USC_1918 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998755 SC_USC_1918 LID_SC_USC_1918 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584993 LID_SC_USC_1919 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998756 SC_USC_1919 LID_SC_USC_1919 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584994 LID_SC_USC_1920 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998757 SC_USC_1920 LID_SC_USC_1920 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584995 LID_SC_USC_1921 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998758 SC_USC_1921 LID_SC_USC_1921 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584996 LID_SC_USC_1922 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998759 SC_USC_1922 LID_SC_USC_1922 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584997 LID_SC_USC_1923 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998760 SC_USC_1923 LID_SC_USC_1923 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2584998 LID_SC_USC_1924 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998761 SC_USC_1924 LID_SC_USC_1924 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2000 alignment_software STAR SRX2584999 LID_SC_USC_1925 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998762 SC_USC_1925 LID_SC_USC_1925 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585000 LID_SC_USC_1926 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998763 SC_USC_1926 LID_SC_USC_1926 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585001 LID_SC_USC_1927 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998765 SC_USC_1927 LID_SC_USC_1927 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2000 alignment_software STAR SRX2585002 LID_SC_USC_1928 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998764 SC_USC_1928 LID_SC_USC_1928 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2000 alignment_software STAR SRX2585003 LID_SC_USC_1929 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998767 SC_USC_1929 LID_SC_USC_1929 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585004 LID_SC_USC_1930 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998766 SC_USC_1930 LID_SC_USC_1930 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585005 LID_SC_USC_1931 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998768 SC_USC_1931 LID_SC_USC_1931 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585006 LID_SC_USC_1932 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998769 SC_USC_1932 LID_SC_USC_1932 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585007 LID_SC_USC_1933 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998770 SC_USC_1933 LID_SC_USC_1933 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585008 LID_SC_USC_1934 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998771 SC_USC_1934 LID_SC_USC_1934 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585009 LID_SC_USC_1935 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998772 SC_USC_1935 LID_SC_USC_1935 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585010 LID_SC_USC_1936 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998773 SC_USC_1936 LID_SC_USC_1936 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585011 LID_SC_USC_1937 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998777 SC_USC_1937 LID_SC_USC_1937 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585012 LID_SC_USC_1938 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998776 SC_USC_1938 LID_SC_USC_1938 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585013 LID_SC_USC_1939 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998774 SC_USC_1939 LID_SC_USC_1939 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585014 LID_SC_USC_1940 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998775 SC_USC_1940 LID_SC_USC_1940 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585015 LID_SC_USC_1941 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998778 SC_USC_1941 LID_SC_USC_1941 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585016 LID_SC_USC_1942 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998781 SC_USC_1942 LID_SC_USC_1942 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585017 LID_SC_USC_1943 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998779 SC_USC_1943 LID_SC_USC_1943 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585018 LID_SC_USC_1944 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998780 SC_USC_1944 LID_SC_USC_1944 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585019 LID_SC_USC_1945 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998782 SC_USC_1945 LID_SC_USC_1945 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585020 LID_SC_USC_1946 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998783 SC_USC_1946 LID_SC_USC_1946 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585021 LID_SC_USC_1947 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998785 SC_USC_1947 LID_SC_USC_1947 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585022 LID_SC_USC_1948 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998784 SC_USC_1948 LID_SC_USC_1948 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585023 LID_SC_USC_1949 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998786 SC_USC_1949 LID_SC_USC_1949 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585024 LID_SC_USC_1950 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998788 SC_USC_1950 LID_SC_USC_1950 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585025 LID_SC_USC_1951 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998787 SC_USC_1951 LID_SC_USC_1951 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585026 LID_SC_USC_1952 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998790 SC_USC_1952 LID_SC_USC_1952 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585027 LID_SC_USC_1953 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998789 SC_USC_1953 LID_SC_USC_1953 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585028 LID_SC_USC_1954 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998791 SC_USC_1954 LID_SC_USC_1954 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585029 LID_SC_USC_1955 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998793 SC_USC_1955 LID_SC_USC_1955 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585030 LID_SC_USC_1956 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998792 SC_USC_1956 LID_SC_USC_1956 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585031 LID_SC_USC_1957 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998794 SC_USC_1957 LID_SC_USC_1957 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585032 LID_SC_USC_1958 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998795 SC_USC_1958 LID_SC_USC_1958 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585033 LID_SC_USC_1959 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998796 SC_USC_1959 LID_SC_USC_1959 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585034 LID_SC_USC_1960 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998798 SC_USC_1960 LID_SC_USC_1960 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585035 LID_SC_USC_1961 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998797 SC_USC_1961 LID_SC_USC_1961 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585036 LID_SC_USC_1962 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998799 SC_USC_1962 LID_SC_USC_1962 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585037 LID_SC_USC_1963 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998816 SC_USC_1963 LID_SC_USC_1963 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585038 LID_SC_USC_1964 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998800 SC_USC_1964 LID_SC_USC_1964 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585039 LID_SC_USC_1965 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998819 SC_USC_1965 LID_SC_USC_1965 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585040 LID_SC_USC_1966 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998801 SC_USC_1966 LID_SC_USC_1966 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585041 LID_SC_USC_1967 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998802 SC_USC_1967 LID_SC_USC_1967 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585042 LID_SC_USC_1968 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998803 SC_USC_1968 LID_SC_USC_1968 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585043 LID_SC_USC_1969 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998804 SC_USC_1969 LID_SC_USC_1969 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585044 LID_SC_USC_1970 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998805 SC_USC_1970 LID_SC_USC_1970 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585045 LID_SC_USC_1971 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998806 SC_USC_1971 LID_SC_USC_1971 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585046 LID_SC_USC_1972 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998807 SC_USC_1972 LID_SC_USC_1972 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585047 LID_SC_USC_1973 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998809 SC_USC_1973 LID_SC_USC_1973 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585048 LID_SC_USC_1974 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998808 SC_USC_1974 LID_SC_USC_1974 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585049 LID_SC_USC_1975 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998810 SC_USC_1975 LID_SC_USC_1975 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585050 LID_SC_USC_1976 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998811 SC_USC_1976 LID_SC_USC_1976 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585051 LID_SC_USC_1977 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998812 SC_USC_1977 LID_SC_USC_1977 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585052 LID_SC_USC_1978 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998813 SC_USC_1978 LID_SC_USC_1978 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585053 LID_SC_USC_1979 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998814 SC_USC_1979 LID_SC_USC_1979 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585054 LID_SC_USC_1980 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998815 SC_USC_1980 LID_SC_USC_1980 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585055 LID_SC_USC_1981 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998817 SC_USC_1981 LID_SC_USC_1981 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585056 LID_SC_USC_1982 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998818 SC_USC_1982 LID_SC_USC_1982 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585057 LID_SC_USC_1983 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998821 SC_USC_1983 LID_SC_USC_1983 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585058 LID_SC_USC_1984 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998822 SC_USC_1984 LID_SC_USC_1984 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585059 LID_SC_USC_1985 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998820 SC_USC_1985 LID_SC_USC_1985 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585060 LID_SC_USC_1986 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998823 SC_USC_1986 LID_SC_USC_1986 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585061 LID_SC_USC_1987 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998824 SC_USC_1987 LID_SC_USC_1987 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585062 LID_SC_USC_1988 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998829 SC_USC_1988 LID_SC_USC_1988 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585063 LID_SC_USC_1989 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998825 SC_USC_1989 LID_SC_USC_1989 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585064 LID_SC_USC_1990 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998826 SC_USC_1990 LID_SC_USC_1990 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585065 LID_SC_USC_1991 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998830 SC_USC_1991 LID_SC_USC_1991 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585066 LID_SC_USC_1992 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998827 SC_USC_1992 LID_SC_USC_1992 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585067 LID_SC_USC_1993 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998828 SC_USC_1993 LID_SC_USC_1993 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585068 LID_SC_USC_1994 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998831 SC_USC_1994 LID_SC_USC_1994 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585069 LID_SC_USC_1995 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998832 SC_USC_1995 LID_SC_USC_1995 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585070 LID_SC_USC_1996 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998833 SC_USC_1996 LID_SC_USC_1996 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585071 LID_SC_USC_1997 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998834 SC_USC_1997 LID_SC_USC_1997 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585072 LID_SC_USC_1998 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998835 SC_USC_1998 LID_SC_USC_1998 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585073 LID_SC_USC_1999 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998837 SC_USC_1999 LID_SC_USC_1999 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585074 LID_SC_USC_2000 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998836 SC_USC_2000 LID_SC_USC_2000 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585075 LID_SC_USC_2001 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998843 SC_USC_2001 LID_SC_USC_2001 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585076 LID_SC_USC_2002 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998845 SC_USC_2002 LID_SC_USC_2002 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585077 LID_SC_USC_2003 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998841 SC_USC_2003 LID_SC_USC_2003 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585078 LID_SC_USC_2004 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998838 SC_USC_2004 LID_SC_USC_2004 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585079 LID_SC_USC_2005 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998839 SC_USC_2005 LID_SC_USC_2005 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585080 LID_SC_USC_2006 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998842 SC_USC_2006 LID_SC_USC_2006 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585081 LID_SC_USC_2007 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998840 SC_USC_2007 LID_SC_USC_2007 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585082 LID_SC_USC_2008 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998844 SC_USC_2008 LID_SC_USC_2008 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585083 LID_SC_USC_2009 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998852 SC_USC_2009 LID_SC_USC_2009 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585084 LID_SC_USC_2010 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998846 SC_USC_2010 LID_SC_USC_2010 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585085 LID_SC_USC_2011 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998847 SC_USC_2011 LID_SC_USC_2011 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585086 LID_SC_USC_2012 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998848 SC_USC_2012 LID_SC_USC_2012 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585087 LID_SC_USC_2013 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998849 SC_USC_2013 LID_SC_USC_2013 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585088 LID_SC_USC_2014 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998850 SC_USC_2014 LID_SC_USC_2014 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585089 LID_SC_USC_2015 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998851 SC_USC_2015 LID_SC_USC_2015 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585090 LID_SC_USC_2016 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998858 SC_USC_2016 LID_SC_USC_2016 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585091 LID_SC_USC_2017 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998855 SC_USC_2017 LID_SC_USC_2017 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585092 LID_SC_USC_2018 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998853 SC_USC_2018 LID_SC_USC_2018 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585093 LID_SC_USC_2019 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998857 SC_USC_2019 LID_SC_USC_2019 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585094 LID_SC_USC_2020 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998856 SC_USC_2020 LID_SC_USC_2020 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585095 LID_SC_USC_2021 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998854 SC_USC_2021 LID_SC_USC_2021 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585096 LID_SC_USC_2022 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998859 SC_USC_2022 LID_SC_USC_2022 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585097 LID_SC_USC_2023 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998861 SC_USC_2023 LID_SC_USC_2023 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585098 LID_SC_USC_2024 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998860 SC_USC_2024 LID_SC_USC_2024 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585099 LID_SC_USC_2025 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998864 SC_USC_2025 LID_SC_USC_2025 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585100 LID_SC_USC_2026 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998863 SC_USC_2026 LID_SC_USC_2026 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585101 LID_SC_USC_2027 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998865 SC_USC_2027 LID_SC_USC_2027 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585102 LID_SC_USC_2028 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998862 SC_USC_2028 LID_SC_USC_2028 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585103 LID_SC_USC_2029 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998866 SC_USC_2029 LID_SC_USC_2029 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585104 LID_SC_USC_2030 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998867 SC_USC_2030 LID_SC_USC_2030 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585105 LID_SC_USC_2031 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998868 SC_USC_2031 LID_SC_USC_2031 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585106 LID_SC_USC_2032 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998869 SC_USC_2032 LID_SC_USC_2032 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585107 LID_SC_USC_2033 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998870 SC_USC_2033 LID_SC_USC_2033 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585108 LID_SC_USC_2034 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998871 SC_USC_2034 LID_SC_USC_2034 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585109 LID_SC_USC_2035 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998873 SC_USC_2035 LID_SC_USC_2035 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585110 LID_SC_USC_2036 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998872 SC_USC_2036 LID_SC_USC_2036 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585111 LID_SC_USC_2037 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998874 SC_USC_2037 LID_SC_USC_2037 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585112 LID_SC_USC_2038 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998875 SC_USC_2038 LID_SC_USC_2038 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585113 LID_SC_USC_2039 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998877 SC_USC_2039 LID_SC_USC_2039 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585114 LID_SC_USC_2040 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998876 SC_USC_2040 LID_SC_USC_2040 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585115 LID_SC_USC_2041 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998878 SC_USC_2041 LID_SC_USC_2041 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585116 LID_SC_USC_2042 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998879 SC_USC_2042 LID_SC_USC_2042 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585117 LID_SC_USC_2043 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998880 SC_USC_2043 LID_SC_USC_2043 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585118 LID_SC_USC_2044 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998881 SC_USC_2044 LID_SC_USC_2044 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585119 LID_SC_USC_2045 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998886 SC_USC_2045 LID_SC_USC_2045 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585120 LID_SC_USC_2046 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998883 SC_USC_2046 LID_SC_USC_2046 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585121 LID_SC_USC_2047 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998884 SC_USC_2047 LID_SC_USC_2047 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585122 LID_SC_USC_2048 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998882 SC_USC_2048 LID_SC_USC_2048 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585123 LID_SC_USC_2049 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998885 SC_USC_2049 LID_SC_USC_2049 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585124 LID_SC_USC_2050 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998887 SC_USC_2050 LID_SC_USC_2050 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585125 LID_SC_USC_2051 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998888 SC_USC_2051 LID_SC_USC_2051 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585126 LID_SC_USC_2052 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998889 SC_USC_2052 LID_SC_USC_2052 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585127 LID_SC_USC_2053 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998891 SC_USC_2053 LID_SC_USC_2053 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585128 LID_SC_USC_2054 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998890 SC_USC_2054 LID_SC_USC_2054 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585129 LID_SC_USC_2055 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998893 SC_USC_2055 LID_SC_USC_2055 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585130 LID_SC_USC_2056 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998892 SC_USC_2056 LID_SC_USC_2056 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585131 LID_SC_USC_2057 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998894 SC_USC_2057 LID_SC_USC_2057 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585132 LID_SC_USC_2058 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998895 SC_USC_2058 LID_SC_USC_2058 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585133 LID_SC_USC_2059 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998896 SC_USC_2059 LID_SC_USC_2059 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585134 LID_SC_USC_2060 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998897 SC_USC_2060 LID_SC_USC_2060 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585135 LID_SC_USC_2061 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998905 SC_USC_2061 LID_SC_USC_2061 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585136 LID_SC_USC_2062 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998898 SC_USC_2062 LID_SC_USC_2062 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585137 LID_SC_USC_2063 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998900 SC_USC_2063 LID_SC_USC_2063 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585138 LID_SC_USC_2064 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998899 SC_USC_2064 LID_SC_USC_2064 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585139 LID_SC_USC_2065 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998901 SC_USC_2065 LID_SC_USC_2065 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585140 LID_SC_USC_2066 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998902 SC_USC_2066 LID_SC_USC_2066 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585141 LID_SC_USC_2067 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998903 SC_USC_2067 LID_SC_USC_2067 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585142 LID_SC_USC_2068 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998904 SC_USC_2068 LID_SC_USC_2068 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585143 LID_SC_USC_2069 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998907 SC_USC_2069 LID_SC_USC_2069 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585144 LID_SC_USC_2070 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998908 SC_USC_2070 LID_SC_USC_2070 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585145 LID_SC_USC_2071 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998906 SC_USC_2071 LID_SC_USC_2071 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585146 LID_SC_USC_2072 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998909 SC_USC_2072 LID_SC_USC_2072 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585147 LID_SC_USC_2073 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998910 SC_USC_2073 LID_SC_USC_2073 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585148 LID_SC_USC_2074 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998911 SC_USC_2074 LID_SC_USC_2074 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585149 LID_SC_USC_2075 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998912 SC_USC_2075 LID_SC_USC_2075 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585150 LID_SC_USC_2076 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998914 SC_USC_2076 LID_SC_USC_2076 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585151 LID_SC_USC_2077 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998913 SC_USC_2077 LID_SC_USC_2077 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585152 LID_SC_USC_2078 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998915 SC_USC_2078 LID_SC_USC_2078 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585153 LID_SC_USC_2079 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998916 SC_USC_2079 LID_SC_USC_2079 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585154 LID_SC_USC_2080 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998917 SC_USC_2080 LID_SC_USC_2080 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585155 LID_SC_USC_2081 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998918 SC_USC_2081 LID_SC_USC_2081 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585156 LID_SC_USC_2082 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998919 SC_USC_2082 LID_SC_USC_2082 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585157 LID_SC_USC_2083 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998921 SC_USC_2083 LID_SC_USC_2083 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585158 LID_SC_USC_2084 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998920 SC_USC_2084 LID_SC_USC_2084 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585159 LID_SC_USC_2085 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998922 SC_USC_2085 LID_SC_USC_2085 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585160 LID_SC_USC_2086 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998925 SC_USC_2086 LID_SC_USC_2086 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585161 LID_SC_USC_2087 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998927 SC_USC_2087 LID_SC_USC_2087 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585162 LID_SC_USC_2088 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998923 SC_USC_2088 LID_SC_USC_2088 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585163 LID_SC_USC_2089 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998928 SC_USC_2089 LID_SC_USC_2089 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585164 LID_SC_USC_2090 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998929 SC_USC_2090 LID_SC_USC_2090 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585165 LID_SC_USC_2091 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998924 SC_USC_2091 LID_SC_USC_2091 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585166 LID_SC_USC_2092 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998930 SC_USC_2092 LID_SC_USC_2092 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585167 LID_SC_USC_2093 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998926 SC_USC_2093 LID_SC_USC_2093 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585168 LID_SC_USC_2094 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998932 SC_USC_2094 LID_SC_USC_2094 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585169 LID_SC_USC_2095 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998933 SC_USC_2095 LID_SC_USC_2095 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585170 LID_SC_USC_2096 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998931 SC_USC_2096 LID_SC_USC_2096 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585171 LID_SC_USC_2097 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998935 SC_USC_2097 LID_SC_USC_2097 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585172 LID_SC_USC_2098 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998934 SC_USC_2098 LID_SC_USC_2098 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585173 LID_SC_USC_2099 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998938 SC_USC_2099 LID_SC_USC_2099 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585174 LID_SC_USC_2100 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998937 SC_USC_2100 LID_SC_USC_2100 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585175 LID_SC_USC_2101 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998936 SC_USC_2101 LID_SC_USC_2101 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585176 LID_SC_USC_2102 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998939 SC_USC_2102 LID_SC_USC_2102 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585177 LID_SC_USC_2103 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998940 SC_USC_2103 LID_SC_USC_2103 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585178 LID_SC_USC_2104 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998941 SC_USC_2104 LID_SC_USC_2104 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585179 LID_SC_USC_2105 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998943 SC_USC_2105 LID_SC_USC_2105 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585180 LID_SC_USC_2106 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998942 SC_USC_2106 LID_SC_USC_2106 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585181 LID_SC_USC_2107 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998944 SC_USC_2107 LID_SC_USC_2107 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585182 LID_SC_USC_2108 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998945 SC_USC_2108 LID_SC_USC_2108 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585183 LID_SC_USC_2109 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998946 SC_USC_2109 LID_SC_USC_2109 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585184 LID_SC_USC_2110 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998947 SC_USC_2110 LID_SC_USC_2110 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585185 LID_SC_USC_2111 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998948 SC_USC_2111 LID_SC_USC_2111 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585186 LID_SC_USC_2112 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998949 SC_USC_2112 LID_SC_USC_2112 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585187 LID_SC_USC_2113 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998951 SC_USC_2113 LID_SC_USC_2113 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585188 LID_SC_USC_2114 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998950 SC_USC_2114 LID_SC_USC_2114 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585189 LID_SC_USC_2115 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998953 SC_USC_2115 LID_SC_USC_2115 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585190 LID_SC_USC_2116 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998952 SC_USC_2116 LID_SC_USC_2116 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585191 LID_SC_USC_2117 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998954 SC_USC_2117 LID_SC_USC_2117 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585192 LID_SC_USC_2118 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998955 SC_USC_2118 LID_SC_USC_2118 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585193 LID_SC_USC_2119 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998956 SC_USC_2119 LID_SC_USC_2119 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585194 LID_SC_USC_2120 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998957 SC_USC_2120 LID_SC_USC_2120 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585195 LID_SC_USC_2121 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998962 SC_USC_2121 LID_SC_USC_2121 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585196 LID_SC_USC_2122 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998959 SC_USC_2122 LID_SC_USC_2122 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585197 LID_SC_USC_2123 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998961 SC_USC_2123 LID_SC_USC_2123 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585198 LID_SC_USC_2124 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998960 SC_USC_2124 LID_SC_USC_2124 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585199 LID_SC_USC_2125 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998958 SC_USC_2125 LID_SC_USC_2125 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585200 LID_SC_USC_2126 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998963 SC_USC_2126 LID_SC_USC_2126 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585201 LID_SC_USC_2127 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998964 SC_USC_2127 LID_SC_USC_2127 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585202 LID_SC_USC_2128 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998965 SC_USC_2128 LID_SC_USC_2128 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585203 LID_SC_USC_2129 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998970 SC_USC_2129 LID_SC_USC_2129 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585204 LID_SC_USC_2130 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998971 SC_USC_2130 LID_SC_USC_2130 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585205 LID_SC_USC_2131 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998972 SC_USC_2131 LID_SC_USC_2131 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585206 LID_SC_USC_2132 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998966 SC_USC_2132 LID_SC_USC_2132 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585207 LID_SC_USC_2133 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998969 SC_USC_2133 LID_SC_USC_2133 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585208 LID_SC_USC_2134 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998967 SC_USC_2134 LID_SC_USC_2134 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585209 LID_SC_USC_2135 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998968 SC_USC_2135 LID_SC_USC_2135 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585210 LID_SC_USC_2136 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998973 SC_USC_2136 LID_SC_USC_2136 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585211 LID_SC_USC_2137 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998974 SC_USC_2137 LID_SC_USC_2137 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585212 LID_SC_USC_2138 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998975 SC_USC_2138 LID_SC_USC_2138 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585213 LID_SC_USC_2139 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998977 SC_USC_2139 LID_SC_USC_2139 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585214 LID_SC_USC_2140 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998976 SC_USC_2140 LID_SC_USC_2140 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585215 LID_SC_USC_2141 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998981 SC_USC_2141 LID_SC_USC_2141 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585216 LID_SC_USC_2142 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998978 SC_USC_2142 LID_SC_USC_2142 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585217 LID_SC_USC_2143 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998979 SC_USC_2143 LID_SC_USC_2143 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585218 LID_SC_USC_2144 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998980 SC_USC_2144 LID_SC_USC_2144 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585219 LID_SC_USC_2145 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998982 SC_USC_2145 LID_SC_USC_2145 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585220 LID_SC_USC_2146 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998983 SC_USC_2146 LID_SC_USC_2146 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585221 LID_SC_USC_2147 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998987 SC_USC_2147 LID_SC_USC_2147 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585222 LID_SC_USC_2148 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998984 SC_USC_2148 LID_SC_USC_2148 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585223 LID_SC_USC_2149 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998985 SC_USC_2149 LID_SC_USC_2149 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585224 LID_SC_USC_2150 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998986 SC_USC_2150 LID_SC_USC_2150 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585225 LID_SC_USC_2151 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998988 SC_USC_2151 LID_SC_USC_2151 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585226 LID_SC_USC_2152 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998990 SC_USC_2152 LID_SC_USC_2152 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585227 LID_SC_USC_2153 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998989 SC_USC_2153 LID_SC_USC_2153 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585228 LID_SC_USC_2154 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998994 SC_USC_2154 LID_SC_USC_2154 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585229 LID_SC_USC_2155 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998991 SC_USC_2155 LID_SC_USC_2155 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585230 LID_SC_USC_2156 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998993 SC_USC_2156 LID_SC_USC_2156 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585231 LID_SC_USC_2157 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998992 SC_USC_2157 LID_SC_USC_2157 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585232 LID_SC_USC_2158 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998996 SC_USC_2158 LID_SC_USC_2158 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585233 LID_SC_USC_2159 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998995 SC_USC_2159 LID_SC_USC_2159 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585234 LID_SC_USC_2160 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998997 SC_USC_2160 LID_SC_USC_2160 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585235 LID_SC_USC_2161 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998998 SC_USC_2161 LID_SC_USC_2161 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585236 LID_SC_USC_2162 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1998999 SC_USC_2162 LID_SC_USC_2162 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585237 LID_SC_USC_2163 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999000 SC_USC_2163 LID_SC_USC_2163 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585238 LID_SC_USC_2164 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999001 SC_USC_2164 LID_SC_USC_2164 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585239 LID_SC_USC_2165 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999004 SC_USC_2165 LID_SC_USC_2165 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585240 LID_SC_USC_2166 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999002 SC_USC_2166 LID_SC_USC_2166 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585241 LID_SC_USC_2167 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999003 SC_USC_2167 LID_SC_USC_2167 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585242 LID_SC_USC_2168 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999005 SC_USC_2168 LID_SC_USC_2168 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585243 LID_SC_USC_2169 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999008 SC_USC_2169 LID_SC_USC_2169 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585244 LID_SC_USC_2170 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999006 SC_USC_2170 LID_SC_USC_2170 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585245 LID_SC_USC_2171 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999009 SC_USC_2171 LID_SC_USC_2171 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585246 LID_SC_USC_2172 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999007 SC_USC_2172 LID_SC_USC_2172 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585247 LID_SC_USC_2173 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999010 SC_USC_2173 LID_SC_USC_2173 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585248 LID_SC_USC_2174 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999012 SC_USC_2174 LID_SC_USC_2174 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585249 LID_SC_USC_2175 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999011 SC_USC_2175 LID_SC_USC_2175 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585250 LID_SC_USC_2176 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999013 SC_USC_2176 LID_SC_USC_2176 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585251 LID_SC_USC_2177 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999016 SC_USC_2177 LID_SC_USC_2177 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585252 LID_SC_USC_2178 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999015 SC_USC_2178 LID_SC_USC_2178 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585253 LID_SC_USC_2179 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999014 SC_USC_2179 LID_SC_USC_2179 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585254 LID_SC_USC_2180 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999017 SC_USC_2180 LID_SC_USC_2180 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585255 LID_SC_USC_2181 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999018 SC_USC_2181 LID_SC_USC_2181 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585256 LID_SC_USC_2182 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999019 SC_USC_2182 LID_SC_USC_2182 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585257 LID_SC_USC_2183 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999025 SC_USC_2183 LID_SC_USC_2183 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585258 LID_SC_USC_2184 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999020 SC_USC_2184 LID_SC_USC_2184 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585259 LID_SC_USC_2185 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999022 SC_USC_2185 LID_SC_USC_2185 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585260 LID_SC_USC_2186 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999021 SC_USC_2186 LID_SC_USC_2186 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585261 LID_SC_USC_2187 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999024 SC_USC_2187 LID_SC_USC_2187 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585262 LID_SC_USC_2188 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999026 SC_USC_2188 LID_SC_USC_2188 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585263 LID_SC_USC_2189 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999023 SC_USC_2189 LID_SC_USC_2189 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585264 LID_SC_USC_2190 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999027 SC_USC_2190 LID_SC_USC_2190 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585265 LID_SC_USC_2191 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999028 SC_USC_2191 LID_SC_USC_2191 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585266 LID_SC_USC_2192 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999029 SC_USC_2192 LID_SC_USC_2192 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585267 LID_SC_USC_2193 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999030 SC_USC_2193 LID_SC_USC_2193 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585268 LID_SC_USC_2194 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999031 SC_USC_2194 LID_SC_USC_2194 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585269 LID_SC_USC_2195 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999038 SC_USC_2195 LID_SC_USC_2195 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585270 LID_SC_USC_2196 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999032 SC_USC_2196 LID_SC_USC_2196 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585271 LID_SC_USC_2197 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999035 SC_USC_2197 LID_SC_USC_2197 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585272 LID_SC_USC_2198 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999036 SC_USC_2198 LID_SC_USC_2198 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585273 LID_SC_USC_2199 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999037 SC_USC_2199 LID_SC_USC_2199 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585274 LID_SC_USC_2200 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999033 SC_USC_2200 LID_SC_USC_2200 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585275 LID_SC_USC_2201 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999040 SC_USC_2201 LID_SC_USC_2201 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585276 LID_SC_USC_2202 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999034 SC_USC_2202 LID_SC_USC_2202 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585277 LID_SC_USC_2203 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999039 SC_USC_2203 LID_SC_USC_2203 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585278 LID_SC_USC_2204 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999042 SC_USC_2204 LID_SC_USC_2204 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585279 LID_SC_USC_2205 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999048 SC_USC_2205 LID_SC_USC_2205 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585280 LID_SC_USC_2206 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999046 SC_USC_2206 LID_SC_USC_2206 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585281 LID_SC_USC_2207 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999041 SC_USC_2207 LID_SC_USC_2207 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585282 LID_SC_USC_2208 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999049 SC_USC_2208 LID_SC_USC_2208 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585283 LID_SC_USC_2209 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999047 SC_USC_2209 LID_SC_USC_2209 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585284 LID_SC_USC_2210 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999043 SC_USC_2210 LID_SC_USC_2210 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585285 LID_SC_USC_2211 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999044 SC_USC_2211 LID_SC_USC_2211 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585286 LID_SC_USC_2212 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999045 SC_USC_2212 LID_SC_USC_2212 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585287 LID_SC_USC_2213 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999051 SC_USC_2213 LID_SC_USC_2213 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585288 LID_SC_USC_2214 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999050 SC_USC_2214 LID_SC_USC_2214 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585289 LID_SC_USC_2215 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999052 SC_USC_2215 LID_SC_USC_2215 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585290 LID_SC_USC_2216 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999053 SC_USC_2216 LID_SC_USC_2216 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585291 LID_SC_USC_2217 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999054 SC_USC_2217 LID_SC_USC_2217 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585292 LID_SC_USC_2218 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999055 SC_USC_2218 LID_SC_USC_2218 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585293 LID_SC_USC_2219 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999056 SC_USC_2219 LID_SC_USC_2219 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585294 LID_SC_USC_2220 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999058 SC_USC_2220 LID_SC_USC_2220 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585295 LID_SC_USC_2221 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999057 SC_USC_2221 LID_SC_USC_2221 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585296 LID_SC_USC_2222 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999059 SC_USC_2222 LID_SC_USC_2222 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585297 LID_SC_USC_2223 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999060 SC_USC_2223 LID_SC_USC_2223 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585298 LID_SC_USC_2224 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999062 SC_USC_2224 LID_SC_USC_2224 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585299 LID_SC_USC_2225 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999061 SC_USC_2225 LID_SC_USC_2225 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585300 LID_SC_USC_2226 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999063 SC_USC_2226 LID_SC_USC_2226 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585301 LID_SC_USC_2227 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999064 SC_USC_2227 LID_SC_USC_2227 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585302 LID_SC_USC_2228 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999065 SC_USC_2228 LID_SC_USC_2228 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585303 LID_SC_USC_2229 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999066 SC_USC_2229 LID_SC_USC_2229 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585304 LID_SC_USC_2230 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999067 SC_USC_2230 LID_SC_USC_2230 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585305 LID_SC_USC_2231 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999070 SC_USC_2231 LID_SC_USC_2231 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585306 LID_SC_USC_2232 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999069 SC_USC_2232 LID_SC_USC_2232 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585307 LID_SC_USC_2233 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999068 SC_USC_2233 LID_SC_USC_2233 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585308 LID_SC_USC_2234 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999071 SC_USC_2234 LID_SC_USC_2234 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585309 LID_SC_USC_2235 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999072 SC_USC_2235 LID_SC_USC_2235 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585310 LID_SC_USC_2236 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999074 SC_USC_2236 LID_SC_USC_2236 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585311 LID_SC_USC_2237 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999073 SC_USC_2237 LID_SC_USC_2237 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585312 LID_SC_USC_2238 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999075 SC_USC_2238 LID_SC_USC_2238 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585313 LID_SC_USC_2239 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999077 SC_USC_2239 LID_SC_USC_2239 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585314 LID_SC_USC_2240 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999076 SC_USC_2240 LID_SC_USC_2240 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585315 LID_SC_USC_2241 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999078 SC_USC_2241 LID_SC_USC_2241 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585316 LID_SC_USC_2242 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999079 SC_USC_2242 LID_SC_USC_2242 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585317 LID_SC_USC_2243 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999082 SC_USC_2243 LID_SC_USC_2243 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585318 LID_SC_USC_2244 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999081 SC_USC_2244 LID_SC_USC_2244 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585319 LID_SC_USC_2245 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999080 SC_USC_2245 LID_SC_USC_2245 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585320 LID_SC_USC_2246 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999083 SC_USC_2246 LID_SC_USC_2246 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585321 LID_SC_USC_2247 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999084 SC_USC_2247 LID_SC_USC_2247 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585322 LID_SC_USC_2248 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999086 SC_USC_2248 LID_SC_USC_2248 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585323 LID_SC_USC_2249 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999085 SC_USC_2249 LID_SC_USC_2249 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585324 LID_SC_USC_2250 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999087 SC_USC_2250 LID_SC_USC_2250 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585325 LID_SC_USC_2251 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999088 SC_USC_2251 LID_SC_USC_2251 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585326 LID_SC_USC_2252 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999089 SC_USC_2252 LID_SC_USC_2252 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585327 LID_SC_USC_2253 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999090 SC_USC_2253 LID_SC_USC_2253 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585328 LID_SC_USC_2254 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999093 SC_USC_2254 LID_SC_USC_2254 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585329 LID_SC_USC_2255 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999091 SC_USC_2255 LID_SC_USC_2255 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585330 LID_SC_USC_2256 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999092 SC_USC_2256 LID_SC_USC_2256 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585331 LID_SC_USC_2257 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999094 SC_USC_2257 LID_SC_USC_2257 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585332 LID_SC_USC_2258 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999095 SC_USC_2258 LID_SC_USC_2258 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585333 LID_SC_USC_2259 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999101 SC_USC_2259 LID_SC_USC_2259 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585334 LID_SC_USC_2260 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999100 SC_USC_2260 LID_SC_USC_2260 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585335 LID_SC_USC_2261 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999096 SC_USC_2261 LID_SC_USC_2261 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585336 LID_SC_USC_2262 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999097 SC_USC_2262 LID_SC_USC_2262 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585337 LID_SC_USC_2263 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999102 SC_USC_2263 LID_SC_USC_2263 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585338 LID_SC_USC_2264 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999104 SC_USC_2264 LID_SC_USC_2264 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585339 LID_SC_USC_2265 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999103 SC_USC_2265 LID_SC_USC_2265 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585340 LID_SC_USC_2266 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999098 SC_USC_2266 LID_SC_USC_2266 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585341 LID_SC_USC_2267 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999099 SC_USC_2267 LID_SC_USC_2267 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585342 LID_SC_USC_2268 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999108 SC_USC_2268 LID_SC_USC_2268 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585343 LID_SC_USC_2269 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999105 SC_USC_2269 LID_SC_USC_2269 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585344 LID_SC_USC_2270 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999107 SC_USC_2270 LID_SC_USC_2270 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585345 LID_SC_USC_2271 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999106 SC_USC_2271 LID_SC_USC_2271 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585346 LID_SC_USC_2272 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999109 SC_USC_2272 LID_SC_USC_2272 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585347 LID_SC_USC_2273 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999110 SC_USC_2273 LID_SC_USC_2273 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585348 LID_SC_USC_2274 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999115 SC_USC_2274 LID_SC_USC_2274 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585349 LID_SC_USC_2275 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999111 SC_USC_2275 LID_SC_USC_2275 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585350 LID_SC_USC_2276 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999112 SC_USC_2276 LID_SC_USC_2276 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585351 LID_SC_USC_2277 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999113 SC_USC_2277 LID_SC_USC_2277 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585352 LID_SC_USC_2278 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999114 SC_USC_2278 LID_SC_USC_2278 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585353 LID_SC_USC_2279 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999116 SC_USC_2279 LID_SC_USC_2279 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585354 LID_SC_USC_2280 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999117 SC_USC_2280 LID_SC_USC_2280 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585355 LID_SC_USC_2281 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999118 SC_USC_2281 LID_SC_USC_2281 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585356 LID_SC_USC_2282 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999119 SC_USC_2282 LID_SC_USC_2282 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585357 LID_SC_USC_2283 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999120 SC_USC_2283 LID_SC_USC_2283 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585358 LID_SC_USC_2284 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999121 SC_USC_2284 LID_SC_USC_2284 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585359 LID_SC_USC_2285 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999122 SC_USC_2285 LID_SC_USC_2285 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585360 LID_SC_USC_2286 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999123 SC_USC_2286 LID_SC_USC_2286 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585361 LID_SC_USC_2287 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999125 SC_USC_2287 LID_SC_USC_2287 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585362 LID_SC_USC_2288 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999124 SC_USC_2288 LID_SC_USC_2288 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585363 LID_SC_USC_2289 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999126 SC_USC_2289 LID_SC_USC_2289 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585364 LID_SC_USC_2290 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999127 SC_USC_2290 LID_SC_USC_2290 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585365 LID_SC_USC_2291 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999129 SC_USC_2291 LID_SC_USC_2291 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585366 LID_SC_USC_2292 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999128 SC_USC_2292 LID_SC_USC_2292 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585367 LID_SC_USC_2293 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999130 SC_USC_2293 LID_SC_USC_2293 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585368 LID_SC_USC_2294 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999132 SC_USC_2294 LID_SC_USC_2294 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585369 LID_SC_USC_2295 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999131 SC_USC_2295 LID_SC_USC_2295 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585370 LID_SC_USC_2296 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999133 SC_USC_2296 LID_SC_USC_2296 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585371 LID_SC_USC_2297 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999134 SC_USC_2297 LID_SC_USC_2297 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585372 LID_SC_USC_2298 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999137 SC_USC_2298 LID_SC_USC_2298 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585373 LID_SC_USC_2299 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999135 SC_USC_2299 LID_SC_USC_2299 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585374 LID_SC_USC_2300 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999136 SC_USC_2300 LID_SC_USC_2300 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585375 LID_SC_USC_2301 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999138 SC_USC_2301 LID_SC_USC_2301 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585376 LID_SC_USC_2302 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999139 SC_USC_2302 LID_SC_USC_2302 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585377 LID_SC_USC_2303 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999140 SC_USC_2303 LID_SC_USC_2303 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585378 LID_SC_USC_2304 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999141 SC_USC_2304 LID_SC_USC_2304 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585379 LID_SC_USC_2305 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999143 SC_USC_2305 LID_SC_USC_2305 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585380 LID_SC_USC_2306 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999142 SC_USC_2306 LID_SC_USC_2306 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585381 LID_SC_USC_2307 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999144 SC_USC_2307 LID_SC_USC_2307 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585382 LID_SC_USC_2308 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999145 SC_USC_2308 LID_SC_USC_2308 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585383 LID_SC_USC_2309 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999146 SC_USC_2309 LID_SC_USC_2309 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585384 LID_SC_USC_2310 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999147 SC_USC_2310 LID_SC_USC_2310 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585385 LID_SC_USC_2311 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999148 SC_USC_2311 LID_SC_USC_2311 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585386 LID_SC_USC_2312 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999149 SC_USC_2312 LID_SC_USC_2312 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585387 LID_SC_USC_2313 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999150 SC_USC_2313 LID_SC_USC_2313 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585388 LID_SC_USC_2314 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999151 SC_USC_2314 LID_SC_USC_2314 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585389 LID_SC_USC_2315 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999153 SC_USC_2315 LID_SC_USC_2315 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585390 LID_SC_USC_2316 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999152 SC_USC_2316 LID_SC_USC_2316 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585391 LID_SC_USC_2317 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999154 SC_USC_2317 LID_SC_USC_2317 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585392 LID_SC_USC_2318 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999155 SC_USC_2318 LID_SC_USC_2318 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585393 LID_SC_USC_2319 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999156 SC_USC_2319 LID_SC_USC_2319 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585394 LID_SC_USC_2320 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999158 SC_USC_2320 LID_SC_USC_2320 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585395 LID_SC_USC_2321 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999157 SC_USC_2321 LID_SC_USC_2321 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585396 LID_SC_USC_2322 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999160 SC_USC_2322 LID_SC_USC_2322 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585397 LID_SC_USC_2323 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999159 SC_USC_2323 LID_SC_USC_2323 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585398 LID_SC_USC_2324 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999161 SC_USC_2324 LID_SC_USC_2324 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585399 LID_SC_USC_2325 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999162 SC_USC_2325 LID_SC_USC_2325 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585400 LID_SC_USC_2326 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999163 SC_USC_2326 LID_SC_USC_2326 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585401 LID_SC_USC_2327 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999164 SC_USC_2327 LID_SC_USC_2327 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR SRX2585402 LID_SC_USC_2328 SRP051388 phs000836 "Single cell RNA was harvested, subjected to aRNA amplification using oligo-dT primers containing T7 promoters as previously described (J Vis Exp. 2011, 50: 2634), with minor modifications. Specifically, we reduced the volume of reactions two times as compared to the method described in the article, and used bead purification instead of ethanol precipitation and RNA column purification. Sequencing library was prepared from 150-400ng aRNA using the TruSeq Stranded mRNA Sample Preparation Kit (illumina, CA, USA) according to manufacturer's protocol. Briefly, cDNA was synthesized using reverse transcriptase (SuperScript III) and random primers. The cDNA was further converted into double stranded DNA, blunt-ended and adenylated at 3'-end. The resulting dsDNA fragments were ligated to double stranded adapters and enriched with PCR using illumina primers. After cleaning up using AMPure XP beads (Beckman, CA, USA), quality control was performed on the resulting library using an Agilent Technologies 2200 TapeStation to characterize DNA fragment size and concentration. The library was subjected to Illumina sequencing platforms on Hiseq2000 and Hiseq2500. Reads were then demultiplexed by index sorting." SRS1999165 SC_USC_2328 LID_SC_USC_2328 RNA-Seq TRANSCRIPTOMIC RANDOM 101 0 Application Read Forward 1 Illumina HiSeq 2500 alignment_software STAR