SRX2749558 L002749 SRP104455 PRJNA380953 Cell suspensions were loaded on a GemCode Single Cell Instrument to generate single cell Gel bead in Emulsions (GEMs). Upon encapsulation, cells lysis benign and gel beads automatically dissolve to release their oligonucleotides for reverse transcription of poly-adneylated RNAs. Each cDNA molecule contains a UMI and shared barcode per GEM after RT. Subsequently, the emulsions were broken and barcoded cDNAs were pooled for PCR amplification. Amplified cDNAs were shared to ~ 200bp using a Covaris E210 system. After end repair and A-tailing, adapters were ligated to product, followed by PCR to incorporate sample indices. SRS2135625 SAMN06657350 L002749 RNA-Seq TRANSCRIPTOMIC size fractionation Illumina HiSeq 2500 SRX2749559 L002668 SRP104455 PRJNA380953 Cell suspensions were loaded on a GemCode Single Cell Instrument to generate single cell Gel bead in Emulsions (GEMs). Upon encapsulation, cells lysis benign and gel beads automatically dissolve to release their oligonucleotides for reverse transcription of poly-adneylated RNAs. Each cDNA molecule contains a UMI and shared barcode per GEM after RT. Subsequently, the emulsions were broken and barcoded cDNAs were pooled for PCR amplification. Amplified cDNAs were shared to ~ 200bp using a Covaris E210 system. After end repair and A-tailing, adapters were ligated to product, followed by PCR to incorporate sample indices. SRS2135626 SAMN06657349 L002668 RNA-Seq TRANSCRIPTOMIC size fractionation Illumina HiSeq 2500 SRX2749560 L002598 SRP104455 PRJNA380953 Cell suspensions were loaded on a GemCode Single Cell Instrument to generate single cell Gel bead in Emulsions (GEMs). Upon encapsulation, cells lysis benign and gel beads automatically dissolve to release their oligonucleotides for reverse transcription of poly-adneylated RNAs. Each cDNA molecule contains a UMI and shared barcode per GEM after RT. Subsequently, the emulsions were broken and barcoded cDNAs were pooled for PCR amplification. Amplified cDNAs were shared to ~ 200bp using a Covaris E210 system. After end repair and A-tailing, adapters were ligated to product, followed by PCR to incorporate sample indices. SRS2135627 SAMN06657348 L002598 RNA-Seq TRANSCRIPTOMIC size fractionation Illumina HiSeq 2500