SRX2763788 GSM2588868 SRP105184 SRS2148352 GSM2588868 RNA-Seq TRANSCRIPTOMIC cDNA Cell-sorted lung macrophages were lysed using QIAshredder Mini Spin Column (Qiagen, #79656) and RNA was extracted using RNeasy Mini Kit (Qiagen, #74106) following the manufacturer's instructions. mRNA was purified from approximately 500ng of total RNA with oligo-dT beads and sheared by incubation at 94C. Following first-strand synthesis with random primers, second strand synthesis was performed with dUTP for generating strand-specific sequencing libraries. The cDNA library was then end-repaired, and A-tailed, adapters were ligated and second-strand digestion was performed by Uracil-DNA-Glycosylase. Indexed libraries that met appropriate cut-offs for both were quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution determined with the LabChip GX or Agilent Bioanalyzer. Samples with a yield of ≥0.5 ng/ul were used for sequencing. Illumina HiSeq 2500 gds 302588868 GSM2588868 GEO Accession GSM2588868 SRX2763789 GSM2588869 SRP105184 SRS2148353 GSM2588869 RNA-Seq TRANSCRIPTOMIC cDNA Cell-sorted lung macrophages were lysed using QIAshredder Mini Spin Column (Qiagen, #79656) and RNA was extracted using RNeasy Mini Kit (Qiagen, #74106) following the manufacturer's instructions. mRNA was purified from approximately 500ng of total RNA with oligo-dT beads and sheared by incubation at 94C. Following first-strand synthesis with random primers, second strand synthesis was performed with dUTP for generating strand-specific sequencing libraries. The cDNA library was then end-repaired, and A-tailed, adapters were ligated and second-strand digestion was performed by Uracil-DNA-Glycosylase. Indexed libraries that met appropriate cut-offs for both were quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution determined with the LabChip GX or Agilent Bioanalyzer. Samples with a yield of ≥0.5 ng/ul were used for sequencing. Illumina HiSeq 2500 gds 302588869 GSM2588869 GEO Accession GSM2588869