SRX2765956 miraBRE_H3K9ac SRP034587 PRJNA219440 DNA (30 ng) from native ChIP was polished to generate blunt ended fragments, add 3_-dA overhangs by treatment with Klenow polymerase and ligated to adapters as described [21]. Fragments were size selected (150Đ500bp) after migration through a 2% NuSieve agarose gel and sequencing libraries were generated by PCR amplification with 1 __l sample DNA, 1_ Phusion polymerase mix (Finnzymes, #F531), 2.25 __M of each Solexa PCR primer. We used a standard protocol: denaturation, 90 _ C, 30 s; amplification (repeated 18 times), 98 _C for 10 s, 65 _C for 30 s, 72 _C for 30 s; final elongation, 72 _ C, 5 min. PCR products were purified on QIAquick PCR Purification columns (Qiagen, #28104) and eluted in 30__l Qiagen elution buffer. We quantified the DNA library with a Nan- odrop1000 spectrophotometer (Thermo Scientific) and clusters were generated with 4pM of the 10nM library dilution on the Illumina cluster station. A 36-cycle sequencing kit on the Illumina 1G analyzer was used according to the manufacturerŐs instructions SRS2150043 miracidia_both_BRE_chip_H3K9ac miraBRE_H3K9ac ChIP-Seq GENOMIC ChIP Illumina Genome Analyzer