SRX2766934 GSM2590462 SRP105341 SRS2151049 GSM2590462 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitation was performed as previously described (Aparicio, Geisberg et al. 2005). Briefly, yeast cells were grown in 500 ml of SC medium to an OD600 of 0.8–0.9 and were crosslinked with 1% formaldehyde (Sigma-Aldrich, Saint Louis, MO) for 20 minutes before the chromatin was extracted. The chromatin was sonicated using Bioruptor (Diagenode, Denville, NJ) at the high setting for ten cycles of 30 sec on/off to yield an average DNA fragment size of 500 bp. Chromatin extracts were diluted in immunoprecipitation (IP) buffer and centrifuged to pellet debris; the resulting supernatant, containing the chromatin solution, was aliquoted for immunoprecipitation as follows. Chromatin was first incubated overnight with antibody at 4°C, then with Dynabeads® protein G beads (Thermo Fisher Scientific, Waltham, MA). Beads were washed several times, and DNA was recovered in elution buffer (1% SDS, 0.1 M NaHCO3). Crosslinking was reversed by incubating samples at 65°C overnight, followed by protease treatment, phenol/chloroform extraction, and ethanol precipitation of the recovered DNA. Sequence libraries were constructed and validated using the Illumina library protocol and sequenced using the Illumina HiSeq 2000 system as 50 bp single-end reads. Illumina HiSeq 2500 gds 302590462 GSM2590462 GEO Accession GSM2590462 SRX2766935 GSM2590463 SRP105341 SRS2151050 GSM2590463 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitation was performed as previously described (Aparicio, Geisberg et al. 2005). Briefly, yeast cells were grown in 500 ml of SC medium to an OD600 of 0.8–0.9 and were crosslinked with 1% formaldehyde (Sigma-Aldrich, Saint Louis, MO) for 20 minutes before the chromatin was extracted. The chromatin was sonicated using Bioruptor (Diagenode, Denville, NJ) at the high setting for ten cycles of 30 sec on/off to yield an average DNA fragment size of 500 bp. Chromatin extracts were diluted in immunoprecipitation (IP) buffer and centrifuged to pellet debris; the resulting supernatant, containing the chromatin solution, was aliquoted for immunoprecipitation as follows. Chromatin was first incubated overnight with antibody at 4°C, then with Dynabeads® protein G beads (Thermo Fisher Scientific, Waltham, MA). Beads were washed several times, and DNA was recovered in elution buffer (1% SDS, 0.1 M NaHCO3). Crosslinking was reversed by incubating samples at 65°C overnight, followed by protease treatment, phenol/chloroform extraction, and ethanol precipitation of the recovered DNA. Sequence libraries were constructed and validated using the Illumina library protocol and sequenced using the Illumina HiSeq 2000 system as 50 bp single-end reads. Illumina HiSeq 2500 gds 302590463 GSM2590463 GEO Accession GSM2590463 SRX2766936 GSM2590464 SRP105341 SRS2151051 GSM2590464 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitation was performed as previously described (Aparicio, Geisberg et al. 2005). Briefly, yeast cells were grown in 500 ml of SC medium to an OD600 of 0.8–0.9 and were crosslinked with 1% formaldehyde (Sigma-Aldrich, Saint Louis, MO) for 20 minutes before the chromatin was extracted. The chromatin was sonicated using Bioruptor (Diagenode, Denville, NJ) at the high setting for ten cycles of 30 sec on/off to yield an average DNA fragment size of 500 bp. Chromatin extracts were diluted in immunoprecipitation (IP) buffer and centrifuged to pellet debris; the resulting supernatant, containing the chromatin solution, was aliquoted for immunoprecipitation as follows. Chromatin was first incubated overnight with antibody at 4°C, then with Dynabeads® protein G beads (Thermo Fisher Scientific, Waltham, MA). Beads were washed several times, and DNA was recovered in elution buffer (1% SDS, 0.1 M NaHCO3). Crosslinking was reversed by incubating samples at 65°C overnight, followed by protease treatment, phenol/chloroform extraction, and ethanol precipitation of the recovered DNA. Sequence libraries were constructed and validated using the Illumina library protocol and sequenced using the Illumina HiSeq 2000 system as 50 bp single-end reads. Illumina HiSeq 2500 gds 302590464 GSM2590464 GEO Accession GSM2590464 SRX2766937 GSM2590465 SRP105341 SRS2151052 GSM2590465 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitation was performed as previously described (Aparicio, Geisberg et al. 2005). Briefly, yeast cells were grown in 500 ml of SC medium to an OD600 of 0.8–0.9 and were crosslinked with 1% formaldehyde (Sigma-Aldrich, Saint Louis, MO) for 20 minutes before the chromatin was extracted. The chromatin was sonicated using Bioruptor (Diagenode, Denville, NJ) at the high setting for ten cycles of 30 sec on/off to yield an average DNA fragment size of 500 bp. Chromatin extracts were diluted in immunoprecipitation (IP) buffer and centrifuged to pellet debris; the resulting supernatant, containing the chromatin solution, was aliquoted for immunoprecipitation as follows. Chromatin was first incubated overnight with antibody at 4°C, then with Dynabeads® protein G beads (Thermo Fisher Scientific, Waltham, MA). Beads were washed several times, and DNA was recovered in elution buffer (1% SDS, 0.1 M NaHCO3). Crosslinking was reversed by incubating samples at 65°C overnight, followed by protease treatment, phenol/chloroform extraction, and ethanol precipitation of the recovered DNA. Sequence libraries were constructed and validated using the Illumina library protocol and sequenced using the Illumina HiSeq 2000 system as 50 bp single-end reads. Illumina HiSeq 2500 gds 302590465 GSM2590465 GEO Accession GSM2590465 SRX2766938 GSM2590466 SRP105341 SRS2151053 GSM2590466 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitation was performed as previously described (Aparicio, Geisberg et al. 2005). Briefly, yeast cells were grown in 500 ml of SC medium to an OD600 of 0.8–0.9 and were crosslinked with 1% formaldehyde (Sigma-Aldrich, Saint Louis, MO) for 20 minutes before the chromatin was extracted. The chromatin was sonicated using Bioruptor (Diagenode, Denville, NJ) at the high setting for ten cycles of 30 sec on/off to yield an average DNA fragment size of 500 bp. Chromatin extracts were diluted in immunoprecipitation (IP) buffer and centrifuged to pellet debris; the resulting supernatant, containing the chromatin solution, was aliquoted for immunoprecipitation as follows. Chromatin was first incubated overnight with antibody at 4°C, then with Dynabeads® protein G beads (Thermo Fisher Scientific, Waltham, MA). Beads were washed several times, and DNA was recovered in elution buffer (1% SDS, 0.1 M NaHCO3). Crosslinking was reversed by incubating samples at 65°C overnight, followed by protease treatment, phenol/chloroform extraction, and ethanol precipitation of the recovered DNA. Sequence libraries were constructed and validated using the Illumina library protocol and sequenced using the Illumina HiSeq 2000 system as 50 bp single-end reads. Illumina HiSeq 2500 gds 302590466 GSM2590466 GEO Accession GSM2590466 SRX2766939 GSM2590467 SRP105341 SRS2151055 GSM2590467 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitation was performed as previously described (Aparicio, Geisberg et al. 2005). Briefly, yeast cells were grown in 500 ml of SC medium to an OD600 of 0.8–0.9 and were crosslinked with 1% formaldehyde (Sigma-Aldrich, Saint Louis, MO) for 20 minutes before the chromatin was extracted. The chromatin was sonicated using Bioruptor (Diagenode, Denville, NJ) at the high setting for ten cycles of 30 sec on/off to yield an average DNA fragment size of 500 bp. Chromatin extracts were diluted in immunoprecipitation (IP) buffer and centrifuged to pellet debris; the resulting supernatant, containing the chromatin solution, was aliquoted for immunoprecipitation as follows. Chromatin was first incubated overnight with antibody at 4°C, then with Dynabeads® protein G beads (Thermo Fisher Scientific, Waltham, MA). Beads were washed several times, and DNA was recovered in elution buffer (1% SDS, 0.1 M NaHCO3). Crosslinking was reversed by incubating samples at 65°C overnight, followed by protease treatment, phenol/chloroform extraction, and ethanol precipitation of the recovered DNA. Sequence libraries were constructed and validated using the Illumina library protocol and sequenced using the Illumina HiSeq 2000 system as 50 bp single-end reads. Illumina HiSeq 2500 gds 302590467 GSM2590467 GEO Accession GSM2590467 SRX2766940 GSM2590468 SRP105341 SRS2151056 GSM2590468 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitation was performed as previously described (Aparicio, Geisberg et al. 2005). Briefly, yeast cells were grown in 500 ml of SC medium to an OD600 of 0.8–0.9 and were crosslinked with 1% formaldehyde (Sigma-Aldrich, Saint Louis, MO) for 20 minutes before the chromatin was extracted. The chromatin was sonicated using Bioruptor (Diagenode, Denville, NJ) at the high setting for ten cycles of 30 sec on/off to yield an average DNA fragment size of 500 bp. Chromatin extracts were diluted in immunoprecipitation (IP) buffer and centrifuged to pellet debris; the resulting supernatant, containing the chromatin solution, was aliquoted for immunoprecipitation as follows. Chromatin was first incubated overnight with antibody at 4°C, then with Dynabeads® protein G beads (Thermo Fisher Scientific, Waltham, MA). Beads were washed several times, and DNA was recovered in elution buffer (1% SDS, 0.1 M NaHCO3). Crosslinking was reversed by incubating samples at 65°C overnight, followed by protease treatment, phenol/chloroform extraction, and ethanol precipitation of the recovered DNA. Sequence libraries were constructed and validated using the Illumina library protocol and sequenced using the Illumina HiSeq 2000 system as 50 bp single-end reads. Illumina HiSeq 2500 gds 302590468 GSM2590468 GEO Accession GSM2590468 SRX2766941 GSM2590469 SRP105341 SRS2151054 GSM2590469 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitation was performed as previously described (Aparicio, Geisberg et al. 2005). Briefly, yeast cells were grown in 500 ml of SC medium to an OD600 of 0.8–0.9 and were crosslinked with 1% formaldehyde (Sigma-Aldrich, Saint Louis, MO) for 20 minutes before the chromatin was extracted. The chromatin was sonicated using Bioruptor (Diagenode, Denville, NJ) at the high setting for ten cycles of 30 sec on/off to yield an average DNA fragment size of 500 bp. Chromatin extracts were diluted in immunoprecipitation (IP) buffer and centrifuged to pellet debris; the resulting supernatant, containing the chromatin solution, was aliquoted for immunoprecipitation as follows. Chromatin was first incubated overnight with antibody at 4°C, then with Dynabeads® protein G beads (Thermo Fisher Scientific, Waltham, MA). Beads were washed several times, and DNA was recovered in elution buffer (1% SDS, 0.1 M NaHCO3). Crosslinking was reversed by incubating samples at 65°C overnight, followed by protease treatment, phenol/chloroform extraction, and ethanol precipitation of the recovered DNA. Sequence libraries were constructed and validated using the Illumina library protocol and sequenced using the Illumina HiSeq 2000 system as 50 bp single-end reads. Illumina HiSeq 2500 gds 302590469 GSM2590469 GEO Accession GSM2590469 SRX2766942 GSM2590470 SRP105341 SRS2151057 GSM2590470 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitation was performed as previously described (Aparicio, Geisberg et al. 2005). Briefly, yeast cells were grown in 500 ml of SC medium to an OD600 of 0.8–0.9 and were crosslinked with 1% formaldehyde (Sigma-Aldrich, Saint Louis, MO) for 20 minutes before the chromatin was extracted. The chromatin was sonicated using Bioruptor (Diagenode, Denville, NJ) at the high setting for ten cycles of 30 sec on/off to yield an average DNA fragment size of 500 bp. Chromatin extracts were diluted in immunoprecipitation (IP) buffer and centrifuged to pellet debris; the resulting supernatant, containing the chromatin solution, was aliquoted for immunoprecipitation as follows. Chromatin was first incubated overnight with antibody at 4°C, then with Dynabeads® protein G beads (Thermo Fisher Scientific, Waltham, MA). Beads were washed several times, and DNA was recovered in elution buffer (1% SDS, 0.1 M NaHCO3). Crosslinking was reversed by incubating samples at 65°C overnight, followed by protease treatment, phenol/chloroform extraction, and ethanol precipitation of the recovered DNA. Sequence libraries were constructed and validated using the Illumina library protocol and sequenced using the Illumina HiSeq 2000 system as 50 bp single-end reads. Illumina HiSeq 2500 gds 302590470 GSM2590470 GEO Accession GSM2590470 SRX2766943 GSM2590471 SRP105341 SRS2151059 GSM2590471 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitation was performed as previously described (Aparicio, Geisberg et al. 2005). Briefly, yeast cells were grown in 500 ml of SC medium to an OD600 of 0.8–0.9 and were crosslinked with 1% formaldehyde (Sigma-Aldrich, Saint Louis, MO) for 20 minutes before the chromatin was extracted. The chromatin was sonicated using Bioruptor (Diagenode, Denville, NJ) at the high setting for ten cycles of 30 sec on/off to yield an average DNA fragment size of 500 bp. Chromatin extracts were diluted in immunoprecipitation (IP) buffer and centrifuged to pellet debris; the resulting supernatant, containing the chromatin solution, was aliquoted for immunoprecipitation as follows. Chromatin was first incubated overnight with antibody at 4°C, then with Dynabeads® protein G beads (Thermo Fisher Scientific, Waltham, MA). Beads were washed several times, and DNA was recovered in elution buffer (1% SDS, 0.1 M NaHCO3). Crosslinking was reversed by incubating samples at 65°C overnight, followed by protease treatment, phenol/chloroform extraction, and ethanol precipitation of the recovered DNA. Sequence libraries were constructed and validated using the Illumina library protocol and sequenced using the Illumina HiSeq 2000 system as 50 bp single-end reads. Illumina HiSeq 2500 gds 302590471 GSM2590471 GEO Accession GSM2590471 SRX2766944 GSM2590472 SRP105341 SRS2151058 GSM2590472 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitation was performed as previously described (Aparicio, Geisberg et al. 2005). Briefly, yeast cells were grown in 500 ml of SC medium to an OD600 of 0.8–0.9 and were crosslinked with 1% formaldehyde (Sigma-Aldrich, Saint Louis, MO) for 20 minutes before the chromatin was extracted. The chromatin was sonicated using Bioruptor (Diagenode, Denville, NJ) at the high setting for ten cycles of 30 sec on/off to yield an average DNA fragment size of 500 bp. Chromatin extracts were diluted in immunoprecipitation (IP) buffer and centrifuged to pellet debris; the resulting supernatant, containing the chromatin solution, was aliquoted for immunoprecipitation as follows. Chromatin was first incubated overnight with antibody at 4°C, then with Dynabeads® protein G beads (Thermo Fisher Scientific, Waltham, MA). Beads were washed several times, and DNA was recovered in elution buffer (1% SDS, 0.1 M NaHCO3). Crosslinking was reversed by incubating samples at 65°C overnight, followed by protease treatment, phenol/chloroform extraction, and ethanol precipitation of the recovered DNA. Sequence libraries were constructed and validated using the Illumina library protocol and sequenced using the Illumina HiSeq 2000 system as 50 bp single-end reads. Illumina HiSeq 2500 gds 302590472 GSM2590472 GEO Accession GSM2590472 SRX2766945 GSM2590473 SRP105341 SRS2151060 GSM2590473 ChIP-Seq GENOMIC ChIP Chromatin immunoprecipitation was performed as previously described (Aparicio, Geisberg et al. 2005). Briefly, yeast cells were grown in 500 ml of SC medium to an OD600 of 0.8–0.9 and were crosslinked with 1% formaldehyde (Sigma-Aldrich, Saint Louis, MO) for 20 minutes before the chromatin was extracted. The chromatin was sonicated using Bioruptor (Diagenode, Denville, NJ) at the high setting for ten cycles of 30 sec on/off to yield an average DNA fragment size of 500 bp. Chromatin extracts were diluted in immunoprecipitation (IP) buffer and centrifuged to pellet debris; the resulting supernatant, containing the chromatin solution, was aliquoted for immunoprecipitation as follows. Chromatin was first incubated overnight with antibody at 4°C, then with Dynabeads® protein G beads (Thermo Fisher Scientific, Waltham, MA). Beads were washed several times, and DNA was recovered in elution buffer (1% SDS, 0.1 M NaHCO3). Crosslinking was reversed by incubating samples at 65°C overnight, followed by protease treatment, phenol/chloroform extraction, and ethanol precipitation of the recovered DNA. Sequence libraries were constructed and validated using the Illumina library protocol and sequenced using the Illumina HiSeq 2000 system as 50 bp single-end reads. Illumina HiSeq 2500 gds 302590473 GSM2590473 GEO Accession GSM2590473