SRX2774783 BRC10-50 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158093 BRC10-50 BRC10-50 WGS GENOMIC PCR Illumina MiSeq SRX2774784 BRC9-50 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158094 BRC9-50 BRC9-50 WGS GENOMIC PCR Illumina MiSeq SRX2774785 BRC8-50 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158095 BRC8-50 BRC8-50 WGS GENOMIC PCR Illumina MiSeq SRX2774786 BRC7-50 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158096 BRC7-50 BRC7-50 WGS GENOMIC PCR Illumina MiSeq SRX2774787 BRC6-50 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158097 BRC6-50 BRC6-50 WGS GENOMIC PCR Illumina MiSeq SRX2774788 BRC5-50 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158098 BRC5-50 BRC5-50 WGS GENOMIC PCR Illumina MiSeq SRX2774789 BRC4-50 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158099 BRC4-50 BRC4-50 WGS GENOMIC PCR Illumina MiSeq SRX2774790 BRC3-50 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158100 BRC3-50 BRC3-50 WGS GENOMIC PCR Illumina MiSeq SRX2774791 BRC1-50 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158101 BRC1-50 BRC1-50 WGS GENOMIC PCR Illumina MiSeq SRX2774792 BR10-50 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158102 BR10-50 BR10-50 WGS GENOMIC PCR Illumina MiSeq SRX2774793 BR9-50 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158103 BR9-50 BR9-50 WGS GENOMIC PCR Illumina MiSeq SRX2774794 BR8-50 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158104 BR8-50 BR8-50 WGS GENOMIC PCR Illumina MiSeq SRX2774795 BR7-50 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158105 BR7-50 BR7-50 WGS GENOMIC PCR Illumina MiSeq SRX2774796 BR6-50 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158106 BR6-50 BR6-50 WGS GENOMIC PCR Illumina MiSeq SRX2774797 BR4-50 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158107 BR4-50 BR4-50 WGS GENOMIC PCR Illumina MiSeq SRX2774798 BR3-50 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158108 BR3-50 BR3-50 WGS GENOMIC PCR Illumina MiSeq SRX2774799 BR2-50 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158110 BR2-50 BR2-50 WGS GENOMIC PCR Illumina MiSeq SRX2774800 BR1-50 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158109 BR1-50 BR1-50 WGS GENOMIC PCR Illumina MiSeq SRX2774801 BN1-50 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158111 BN1-50 BN1-50 WGS GENOMIC PCR Illumina MiSeq SRX2774802 BNC5-100 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158112 BNC5-100 BNC5-100 WGS GENOMIC PCR Illumina MiSeq SRX2774803 BNC4-100 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158113 BNC4-100 BNC4-100 WGS GENOMIC PCR Illumina MiSeq SRX2774804 BNC3-100 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158116 BNC3-100 BNC3-100 WGS GENOMIC PCR Illumina MiSeq SRX2774805 BNC2-100 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158114 BNC2-100 BNC2-100 WGS GENOMIC PCR Illumina MiSeq SRX2774806 BNC1-100 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158115 BNC1-100 BNC1-100 WGS GENOMIC PCR Illumina MiSeq SRX2774807 BN10-100 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158117 BN10-100 BN10-100 WGS GENOMIC PCR Illumina MiSeq SRX2774808 BN9-100 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158118 BN9-100 BN9-100 WGS GENOMIC PCR Illumina MiSeq SRX2774809 BN8-100 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158119 BN8-100 BN8-100 WGS GENOMIC PCR Illumina MiSeq SRX2774810 BN7-100 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158120 BN7-100 BN7-100 WGS GENOMIC PCR Illumina MiSeq SRX2774811 BN6-100 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158121 BN6-100 BN6-100 WGS GENOMIC PCR Illumina MiSeq SRX2774812 BN5-100 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158122 BN5-100 BN5-100 WGS GENOMIC PCR Illumina MiSeq SRX2774813 BN4-100 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158123 BN4-100 BN4-100 WGS GENOMIC PCR Illumina MiSeq SRX2774814 BN3-100 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158124 BN3-100 BN3-100 WGS GENOMIC PCR Illumina MiSeq SRX2774815 BN2-100 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158125 BN2-100 BN2-100 WGS GENOMIC PCR Illumina MiSeq SRX2774816 BN1-100 SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158126 BN1-100 BN1-100 WGS GENOMIC PCR Illumina MiSeq SRX2774817 Ancestor SRP105908 PRJNA385021 Samples were streaked to single colonies on LB agar. Single colonies were then used to inoculate LB liquid media. Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega), and quantified using the Nanodrop 1000 Spectrophotometer and Qubit 2.0 Fluorometer. Sequencing was carried out by Macrogen Korea using an Illumina MiSeq platform with 2x250bp paired end reads. All 15 evolved samples were multiplexed in a single Illumina MiSeq Lane. SRS2158127 Ancestor Ancestor WGS GENOMIC PCR Illumina MiSeq