SRX2789119 GSM2607700 SRP106720 SRS2170758 GSM2607700 Bisulfite-Seq GENOMIC RANDOM Genomic DNA was extracted from 3 dnmt1+/+ and 3 dnmt1-/- medaka at 18 dpf using DNeasy blood and tissue kit (Qiagen). 150ng of DNA was used for the bisulfite reaction using the EZ DNA methylation kit (Zymo Research) and the bisulfite-treated DNA was recovered in 15ml of elution buffer. 1ml of bisulfite-treated DNA solution was used for 30 cycles of PCR amplification with the indicated bisulfite primers in a reaction volume of 20ml; 1ml of the first PCR reaction was used for a nested PCR reaction with P5 and P7 adaptor-attached bisulfite primers. After the nested PCR reactions, amplicons were purified using PCR purification kit (Qiagen) and qualified and quantified by gel electrophoresis, the High Sensitivity DNA kit (Agilent) for the Agilent Bioanalyzer and the Qubit dsDNA HS assay kit (Invitrogen). The eight amplicons derived from the same original source were mixed at 1ng/ml each and 15ml of the mixture were amplified using P5 and P7 primers attached to barcodes using NEBNext DNA library prep kit (New England Biolabs), following the manufacturer«s protocol. The amplicons were purified using AMPure XP (Beckman Coulter) and qualified and quantified using Bioanalyzer and Qubit dsDNA HS assay kit. Finally, all amplicons were mixed at 1ng/ml each and 5 ml of mixed amplicons were used for sequencing in paired-end 150bp mode on 1 lane of MiSeq Sequencing System (Illlumina). Illumina MiSeq gds 302607700 GSM2607700 GEO Accession GSM2607700 SRX2789120 GSM2607701 SRP106720 SRS2170760 GSM2607701 Bisulfite-Seq GENOMIC RANDOM Genomic DNA was extracted from 3 dnmt1+/+ and 3 dnmt1-/- medaka at 18 dpf using DNeasy blood and tissue kit (Qiagen). 150ng of DNA was used for the bisulfite reaction using the EZ DNA methylation kit (Zymo Research) and the bisulfite-treated DNA was recovered in 15ml of elution buffer. 1ml of bisulfite-treated DNA solution was used for 30 cycles of PCR amplification with the indicated bisulfite primers in a reaction volume of 20ml; 1ml of the first PCR reaction was used for a nested PCR reaction with P5 and P7 adaptor-attached bisulfite primers. After the nested PCR reactions, amplicons were purified using PCR purification kit (Qiagen) and qualified and quantified by gel electrophoresis, the High Sensitivity DNA kit (Agilent) for the Agilent Bioanalyzer and the Qubit dsDNA HS assay kit (Invitrogen). The eight amplicons derived from the same original source were mixed at 1ng/ml each and 15ml of the mixture were amplified using P5 and P7 primers attached to barcodes using NEBNext DNA library prep kit (New England Biolabs), following the manufacturer«s protocol. The amplicons were purified using AMPure XP (Beckman Coulter) and qualified and quantified using Bioanalyzer and Qubit dsDNA HS assay kit. Finally, all amplicons were mixed at 1ng/ml each and 5 ml of mixed amplicons were used for sequencing in paired-end 150bp mode on 1 lane of MiSeq Sequencing System (Illlumina). Illumina MiSeq gds 302607701 GSM2607701 GEO Accession GSM2607701 SRX2789121 GSM2607702 SRP106720 SRS2170759 GSM2607702 Bisulfite-Seq GENOMIC RANDOM Genomic DNA was extracted from 3 dnmt1+/+ and 3 dnmt1-/- medaka at 18 dpf using DNeasy blood and tissue kit (Qiagen). 150ng of DNA was used for the bisulfite reaction using the EZ DNA methylation kit (Zymo Research) and the bisulfite-treated DNA was recovered in 15ml of elution buffer. 1ml of bisulfite-treated DNA solution was used for 30 cycles of PCR amplification with the indicated bisulfite primers in a reaction volume of 20ml; 1ml of the first PCR reaction was used for a nested PCR reaction with P5 and P7 adaptor-attached bisulfite primers. After the nested PCR reactions, amplicons were purified using PCR purification kit (Qiagen) and qualified and quantified by gel electrophoresis, the High Sensitivity DNA kit (Agilent) for the Agilent Bioanalyzer and the Qubit dsDNA HS assay kit (Invitrogen). The eight amplicons derived from the same original source were mixed at 1ng/ml each and 15ml of the mixture were amplified using P5 and P7 primers attached to barcodes using NEBNext DNA library prep kit (New England Biolabs), following the manufacturer«s protocol. The amplicons were purified using AMPure XP (Beckman Coulter) and qualified and quantified using Bioanalyzer and Qubit dsDNA HS assay kit. Finally, all amplicons were mixed at 1ng/ml each and 5 ml of mixed amplicons were used for sequencing in paired-end 150bp mode on 1 lane of MiSeq Sequencing System (Illlumina). Illumina MiSeq gds 302607702 GSM2607702 GEO Accession GSM2607702 SRX2789122 GSM2607703 SRP106720 SRS2170761 GSM2607703 Bisulfite-Seq GENOMIC RANDOM Genomic DNA was extracted from 3 dnmt1+/+ and 3 dnmt1-/- medaka at 18 dpf using DNeasy blood and tissue kit (Qiagen). 150ng of DNA was used for the bisulfite reaction using the EZ DNA methylation kit (Zymo Research) and the bisulfite-treated DNA was recovered in 15ml of elution buffer. 1ml of bisulfite-treated DNA solution was used for 30 cycles of PCR amplification with the indicated bisulfite primers in a reaction volume of 20ml; 1ml of the first PCR reaction was used for a nested PCR reaction with P5 and P7 adaptor-attached bisulfite primers. After the nested PCR reactions, amplicons were purified using PCR purification kit (Qiagen) and qualified and quantified by gel electrophoresis, the High Sensitivity DNA kit (Agilent) for the Agilent Bioanalyzer and the Qubit dsDNA HS assay kit (Invitrogen). The eight amplicons derived from the same original source were mixed at 1ng/ml each and 15ml of the mixture were amplified using P5 and P7 primers attached to barcodes using NEBNext DNA library prep kit (New England Biolabs), following the manufacturer«s protocol. The amplicons were purified using AMPure XP (Beckman Coulter) and qualified and quantified using Bioanalyzer and Qubit dsDNA HS assay kit. Finally, all amplicons were mixed at 1ng/ml each and 5 ml of mixed amplicons were used for sequencing in paired-end 150bp mode on 1 lane of MiSeq Sequencing System (Illlumina). Illumina MiSeq gds 302607703 GSM2607703 GEO Accession GSM2607703 SRX2789123 GSM2607704 SRP106720 SRS2170762 GSM2607704 Bisulfite-Seq GENOMIC RANDOM Genomic DNA was extracted from 3 dnmt1+/+ and 3 dnmt1-/- medaka at 18 dpf using DNeasy blood and tissue kit (Qiagen). 150ng of DNA was used for the bisulfite reaction using the EZ DNA methylation kit (Zymo Research) and the bisulfite-treated DNA was recovered in 15ml of elution buffer. 1ml of bisulfite-treated DNA solution was used for 30 cycles of PCR amplification with the indicated bisulfite primers in a reaction volume of 20ml; 1ml of the first PCR reaction was used for a nested PCR reaction with P5 and P7 adaptor-attached bisulfite primers. After the nested PCR reactions, amplicons were purified using PCR purification kit (Qiagen) and qualified and quantified by gel electrophoresis, the High Sensitivity DNA kit (Agilent) for the Agilent Bioanalyzer and the Qubit dsDNA HS assay kit (Invitrogen). The eight amplicons derived from the same original source were mixed at 1ng/ml each and 15ml of the mixture were amplified using P5 and P7 primers attached to barcodes using NEBNext DNA library prep kit (New England Biolabs), following the manufacturer«s protocol. The amplicons were purified using AMPure XP (Beckman Coulter) and qualified and quantified using Bioanalyzer and Qubit dsDNA HS assay kit. Finally, all amplicons were mixed at 1ng/ml each and 5 ml of mixed amplicons were used for sequencing in paired-end 150bp mode on 1 lane of MiSeq Sequencing System (Illlumina). Illumina MiSeq gds 302607704 GSM2607704 GEO Accession GSM2607704 SRX2789124 GSM2607705 SRP106720 SRS2170763 GSM2607705 Bisulfite-Seq GENOMIC RANDOM Genomic DNA was extracted from 3 dnmt1+/+ and 3 dnmt1-/- medaka at 18 dpf using DNeasy blood and tissue kit (Qiagen). 150ng of DNA was used for the bisulfite reaction using the EZ DNA methylation kit (Zymo Research) and the bisulfite-treated DNA was recovered in 15ml of elution buffer. 1ml of bisulfite-treated DNA solution was used for 30 cycles of PCR amplification with the indicated bisulfite primers in a reaction volume of 20ml; 1ml of the first PCR reaction was used for a nested PCR reaction with P5 and P7 adaptor-attached bisulfite primers. After the nested PCR reactions, amplicons were purified using PCR purification kit (Qiagen) and qualified and quantified by gel electrophoresis, the High Sensitivity DNA kit (Agilent) for the Agilent Bioanalyzer and the Qubit dsDNA HS assay kit (Invitrogen). The eight amplicons derived from the same original source were mixed at 1ng/ml each and 15ml of the mixture were amplified using P5 and P7 primers attached to barcodes using NEBNext DNA library prep kit (New England Biolabs), following the manufacturer«s protocol. The amplicons were purified using AMPure XP (Beckman Coulter) and qualified and quantified using Bioanalyzer and Qubit dsDNA HS assay kit. Finally, all amplicons were mixed at 1ng/ml each and 5 ml of mixed amplicons were used for sequencing in paired-end 150bp mode on 1 lane of MiSeq Sequencing System (Illlumina). Illumina MiSeq gds 302607705 GSM2607705 GEO Accession GSM2607705