SRX2882361 GSM2649279 SRP108576 SRS2250829 GSM2649279 MeDIP-Seq GENOMIC 5-methylcytidine antibody DNA was extracted with average concentrations of 370 ng/µl determined by Qubit Fluorometer, micro plate reader, and nanodrop. The extracted DNA achieved a quantity of above the cut off of ≥ 5µg for all samples. Genome DNA was fragmented to 100-500bp by sonication. DNA-end was repaired to overhang a 3'-dA, then adapters were ligated to the end of DNA fragments. Double-stranded DNA was denatured. Then DNA fragments were immunoprecipitated by 5mC antibody. Real-time PCR was used to validate the quality of immunoprecipitation. DNA fragments with proper size (usually 200-300bp, including adaptor sequence) were selected after PCR amplification. Then there was adequate library for sequencing. Illumina HiSeq 2000 gds 302649279 GSM2649279 GEO Accession GSM2649279 SRX2882362 GSM2649280 SRP108576 SRS2250830 GSM2649280 MeDIP-Seq GENOMIC 5-methylcytidine antibody DNA was extracted with average concentrations of 370 ng/µl determined by Qubit Fluorometer, micro plate reader, and nanodrop. The extracted DNA achieved a quantity of above the cut off of ≥ 5µg for all samples. Genome DNA was fragmented to 100-500bp by sonication. DNA-end was repaired to overhang a 3'-dA, then adapters were ligated to the end of DNA fragments. Double-stranded DNA was denatured. Then DNA fragments were immunoprecipitated by 5mC antibody. Real-time PCR was used to validate the quality of immunoprecipitation. DNA fragments with proper size (usually 200-300bp, including adaptor sequence) were selected after PCR amplification. Then there was adequate library for sequencing. Illumina HiSeq 2000 gds 302649280 GSM2649280 GEO Accession GSM2649280 SRX2882363 GSM2649281 SRP108576 SRS2250831 GSM2649281 MeDIP-Seq GENOMIC 5-methylcytidine antibody DNA was extracted with average concentrations of 370 ng/µl determined by Qubit Fluorometer, micro plate reader, and nanodrop. The extracted DNA achieved a quantity of above the cut off of ≥ 5µg for all samples. Genome DNA was fragmented to 100-500bp by sonication. DNA-end was repaired to overhang a 3'-dA, then adapters were ligated to the end of DNA fragments. Double-stranded DNA was denatured. Then DNA fragments were immunoprecipitated by 5mC antibody. Real-time PCR was used to validate the quality of immunoprecipitation. DNA fragments with proper size (usually 200-300bp, including adaptor sequence) were selected after PCR amplification. Then there was adequate library for sequencing. Illumina HiSeq 2000 gds 302649281 GSM2649281 GEO Accession GSM2649281 SRX2882364 GSM2649282 SRP108576 SRS2250832 GSM2649282 MeDIP-Seq GENOMIC 5-methylcytidine antibody DNA was extracted with average concentrations of 370 ng/µl determined by Qubit Fluorometer, micro plate reader, and nanodrop. The extracted DNA achieved a quantity of above the cut off of ≥ 5µg for all samples. Genome DNA was fragmented to 100-500bp by sonication. DNA-end was repaired to overhang a 3'-dA, then adapters were ligated to the end of DNA fragments. Double-stranded DNA was denatured. Then DNA fragments were immunoprecipitated by 5mC antibody. Real-time PCR was used to validate the quality of immunoprecipitation. DNA fragments with proper size (usually 200-300bp, including adaptor sequence) were selected after PCR amplification. Then there was adequate library for sequencing. Illumina HiSeq 2000 gds 302649282 GSM2649282 GEO Accession GSM2649282