SRX2885621 HL_2 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253672 HL_2 HL_2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885622 HL_1 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253673 HL_1 HL_1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885623 HP_1 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253674 HP_1 HP_1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885624 HL_3 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253675 HL_3 HL_3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885625 HP_3 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253676 HP_3 HP_3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885626 HP_2 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253677 HP_2 HP_2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885627 HA_2 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253678 HA_2 HA_2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885628 HA_1 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253679 HA_1 HA_1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885629 HA_4 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253680 HA_4 HA_4 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885630 HA_3 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253681 HA_3 HA_3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885631 CK_2 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253682 CK_2 CK_2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885632 CK_3 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253683 CK_3 CK_3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885633 CK_1 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253684 CK_1 CK_1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885634 AT_3 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253685 AT_3 AT_3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885635 LABL_2 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253686 LABL_2 LABL_2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885636 LABL_1 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253687 LABL_1 LABL_1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885637 LL_1 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253688 LL_1 LL_1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885638 LABL_3 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253689 LABL_3 LABL_3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885639 LL_3 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253690 LL_3 LL_3 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885640 LL_2 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253691 LL_2 LL_2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885641 AT_2 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253692 AT_2 AT_2 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX2885642 AT_1 SRP108683 bp0 The variable region 4 (V4) of the bacterial 16S rRNA gene was amplified with the general 16S rRNA primers 515F (5’-GTGYCAGCMGCCGCGGTA-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’). The PCR reactions were carried out in a total volume of 30 µl which comprised of 15 µl of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 µM of forward and reverse primers and 10 ng of template DNA. SRS2253693 AT_1 AT_1 AMPLICON METAGENOMIC PCR Illumina MiSeq