SRX2902852 GSM2664253 SRP108999 SRS2269410 GSM2664253 RNA-Seq TRANSCRIPTOMIC cDNA The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 120 µl of the lysate was used for total RNA extraction by TRIzol LS Reagent (ThermoFisher scientific, 10296010) 2.5 µg of total RNA was used for isolation of mRNA with the Dynabeads mRNA DIRECT Purification Kit (ThermoFisher scientific, 61011). The entire isolated mRNA sample was used for library generation with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina sequencing (NEB, E7420S). NextSeq 500 gds 302664253 GSM2664253 GEO Accession GSM2664253 SRX2902853 GSM2664254 SRP108999 SRS2269411 GSM2664254 RNA-Seq TRANSCRIPTOMIC cDNA The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 120 µl of the lysate was used for total RNA extraction by TRIzol LS Reagent (ThermoFisher scientific, 10296010) 2.5 µg of total RNA was used for isolation of mRNA with the Dynabeads mRNA DIRECT Purification Kit (ThermoFisher scientific, 61011). The entire isolated mRNA sample was used for library generation with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina sequencing (NEB, E7420S). NextSeq 500 gds 302664254 GSM2664254 GEO Accession GSM2664254 SRX2902854 GSM2664255 SRP108999 SRS2269412 GSM2664255 RNA-Seq TRANSCRIPTOMIC cDNA The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 120 µl of the lysate was used for total RNA extraction by TRIzol LS Reagent (ThermoFisher scientific, 10296010) 2.5 µg of total RNA was used for isolation of mRNA with the Dynabeads mRNA DIRECT Purification Kit (ThermoFisher scientific, 61011). The entire isolated mRNA sample was used for library generation with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina sequencing (NEB, E7420S). NextSeq 500 gds 302664255 GSM2664255 GEO Accession GSM2664255 SRX2902855 GSM2664256 SRP108999 SRS2269413 GSM2664256 OTHER TRANSCRIPTOMIC other The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 240 µl of lysate was incubated with anti-Flag antibody coated magnetic beads and 10000 units of RNase T1 (ThermoFisher scientific, EN0541) to perform digestion of exposed mRNA and ribosome purification simultaneously. 100 µl of Dynabeads protein G coated with 4 µg anti-Flag antibody was used. The lysate-beads-RNase T1 mixture was incubated at 4°C for 6 hours and washed. RNA was extracted from ribosomes bound to the beads by TRIzol Reagent, and the co-precipitant linear acrylamide (ThermoFisher scientific, AM9520) was used to increase the RNA recovery rate. RNA fragments in the range of 30-45 nt was extracted by gel extraction. Library generation for ribosome profiling was performed using NEBNext Small RNA Library Prep Set for Illumina (NEB, E7330S), additional de-phosphorylation and phosphorylation steps were incorporated in the workflow to ensure compatibility with the kit. NextSeq 500 gds 302664256 GSM2664256 GEO Accession GSM2664256 SRX2902856 GSM2664257 SRP108999 SRS2269414 GSM2664257 OTHER TRANSCRIPTOMIC other The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 240 µl of lysate was incubated with anti-Flag antibody coated magnetic beads and 10000 units of RNase T1 (ThermoFisher scientific, EN0541) to perform digestion of exposed mRNA and ribosome purification simultaneously. 100 µl of Dynabeads protein G coated with 4 µg anti-Flag antibody was used. The lysate-beads-RNase T1 mixture was incubated at 4°C for 6 hours and washed. RNA was extracted from ribosomes bound to the beads by TRIzol Reagent, and the co-precipitant linear acrylamide (ThermoFisher scientific, AM9520) was used to increase the RNA recovery rate. RNA fragments in the range of 30-45 nt was extracted by gel extraction. Library generation for ribosome profiling was performed using NEBNext Small RNA Library Prep Set for Illumina (NEB, E7330S), additional de-phosphorylation and phosphorylation steps were incorporated in the workflow to ensure compatibility with the kit. NextSeq 500 gds 302664257 GSM2664257 GEO Accession GSM2664257 SRX2902857 GSM2664258 SRP108999 SRS2269415 GSM2664258 OTHER TRANSCRIPTOMIC other The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 240 µl of lysate was incubated with anti-Flag antibody coated magnetic beads and 10000 units of RNase T1 (ThermoFisher scientific, EN0541) to perform digestion of exposed mRNA and ribosome purification simultaneously. 100 µl of Dynabeads protein G coated with 4 µg anti-Flag antibody was used. The lysate-beads-RNase T1 mixture was incubated at 4°C for 6 hours and washed. RNA was extracted from ribosomes bound to the beads by TRIzol Reagent, and the co-precipitant linear acrylamide (ThermoFisher scientific, AM9520) was used to increase the RNA recovery rate. RNA fragments in the range of 30-45 nt was extracted by gel extraction. Library generation for ribosome profiling was performed using NEBNext Small RNA Library Prep Set for Illumina (NEB, E7330S), additional de-phosphorylation and phosphorylation steps were incorporated in the workflow to ensure compatibility with the kit. NextSeq 500 gds 302664258 GSM2664258 GEO Accession GSM2664258 SRX2902858 GSM2664259 SRP108999 SRS2269416 GSM2664259 OTHER TRANSCRIPTOMIC other The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 180 µl of the lysate was incubated with anti-flag antibody coated magnetic beads to purify ribosomes with associated mRNA. 75 µl of Dynabeads protein G (ThermoFisher scientific, 10004D) was used to coat 3 µg anti-Flag antibody (Sigma, F1804). The lysate-beads mixture was incubated at 4°C with rotation for 2 hours, then washed in buffer (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide), supplemented with 0.1% Triton-X100 (0.1 U/µl SUPERase in RNase Inhibitor (ThermoFisher scientific, AM2696). RNA was extracted from ribosomes bound to the beads by TRIzol Reagent, and the co-precipitant linear acrylamide (ThermoFisher scientific, AM9520) was used to increase the RNA recovery rate. 2.5 µg of total RNA was used for isolation of mRNA with the Dynabeads mRNA DIRECT Purification Kit (ThermoFisher scientific, 61011). The entire isolated mRNA sample was used for library generation with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina sequencing (NEB, E7420S). NextSeq 500 gds 302664259 GSM2664259 GEO Accession GSM2664259 SRX2902859 GSM2664260 SRP108999 SRS2269417 GSM2664260 OTHER TRANSCRIPTOMIC other The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 180 µl of the lysate was incubated with anti-flag antibody coated magnetic beads to purify ribosomes with associated mRNA. 75 µl of Dynabeads protein G (ThermoFisher scientific, 10004D) was used to coat 3 µg anti-Flag antibody (Sigma, F1804). The lysate-beads mixture was incubated at 4°C with rotation for 2 hours, then washed in buffer (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide), supplemented with 0.1% Triton-X100 (0.1 U/µl SUPERase in RNase Inhibitor (ThermoFisher scientific, AM2696). RNA was extracted from ribosomes bound to the beads by TRIzol Reagent, and the co-precipitant linear acrylamide (ThermoFisher scientific, AM9520) was used to increase the RNA recovery rate. 2.5 µg of total RNA was used for isolation of mRNA with the Dynabeads mRNA DIRECT Purification Kit (ThermoFisher scientific, 61011). The entire isolated mRNA sample was used for library generation with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina sequencing (NEB, E7420S). NextSeq 500 gds 302664260 GSM2664260 GEO Accession GSM2664260 SRX2902860 GSM2664261 SRP108999 SRS2269418 GSM2664261 OTHER TRANSCRIPTOMIC other The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 180 µl of the lysate was incubated with anti-flag antibody coated magnetic beads to purify ribosomes with associated mRNA. 75 µl of Dynabeads protein G (ThermoFisher scientific, 10004D) was used to coat 3 µg anti-Flag antibody (Sigma, F1804). The lysate-beads mixture was incubated at 4°C with rotation for 2 hours, then washed in buffer (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide), supplemented with 0.1% Triton-X100 (0.1 U/µl SUPERase in RNase Inhibitor (ThermoFisher scientific, AM2696). RNA was extracted from ribosomes bound to the beads by TRIzol Reagent, and the co-precipitant linear acrylamide (ThermoFisher scientific, AM9520) was used to increase the RNA recovery rate. 2.5 µg of total RNA was used for isolation of mRNA with the Dynabeads mRNA DIRECT Purification Kit (ThermoFisher scientific, 61011). The entire isolated mRNA sample was used for library generation with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina sequencing (NEB, E7420S). NextSeq 500 gds 302664261 GSM2664261 GEO Accession GSM2664261 SRX2902861 GSM2664262 SRP108999 SRS2269420 GSM2664262 RNA-Seq TRANSCRIPTOMIC cDNA The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 120 µl of the lysate was used for total RNA extraction by TRIzol LS Reagent (ThermoFisher scientific, 10296010) 2.5 µg of total RNA was used for isolation of mRNA with the Dynabeads mRNA DIRECT Purification Kit (ThermoFisher scientific, 61011). The entire isolated mRNA sample was used for library generation with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina sequencing (NEB, E7420S). NextSeq 500 gds 302664262 GSM2664262 GEO Accession GSM2664262 SRX2902862 GSM2664263 SRP108999 SRS2269419 GSM2664263 RNA-Seq TRANSCRIPTOMIC cDNA The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 120 µl of the lysate was used for total RNA extraction by TRIzol LS Reagent (ThermoFisher scientific, 10296010) 2.5 µg of total RNA was used for isolation of mRNA with the Dynabeads mRNA DIRECT Purification Kit (ThermoFisher scientific, 61011). The entire isolated mRNA sample was used for library generation with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina sequencing (NEB, E7420S). NextSeq 500 gds 302664263 GSM2664263 GEO Accession GSM2664263 SRX2902863 GSM2664264 SRP108999 SRS2269421 GSM2664264 RNA-Seq TRANSCRIPTOMIC cDNA The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 120 µl of the lysate was used for total RNA extraction by TRIzol LS Reagent (ThermoFisher scientific, 10296010) 2.5 µg of total RNA was used for isolation of mRNA with the Dynabeads mRNA DIRECT Purification Kit (ThermoFisher scientific, 61011). The entire isolated mRNA sample was used for library generation with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina sequencing (NEB, E7420S). NextSeq 500 gds 302664264 GSM2664264 GEO Accession GSM2664264 SRX2902864 GSM2664265 SRP108999 SRS2269422 GSM2664265 OTHER TRANSCRIPTOMIC other The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 240 µl of lysate was incubated with anti-Flag antibody coated magnetic beads and 10000 units of RNase T1 (ThermoFisher scientific, EN0541) to perform digestion of exposed mRNA and ribosome purification simultaneously. 100 µl of Dynabeads protein G coated with 4 µg anti-Flag antibody was used. The lysate-beads-RNase T1 mixture was incubated at 4°C for 6 hours and washed. RNA was extracted from ribosomes bound to the beads by TRIzol Reagent, and the co-precipitant linear acrylamide (ThermoFisher scientific, AM9520) was used to increase the RNA recovery rate. RNA fragments in the range of 30-45 nt was extracted by gel extraction. Library generation for ribosome profiling was performed using NEBNext Small RNA Library Prep Set for Illumina (NEB, E7330S), additional de-phosphorylation and phosphorylation steps were incorporated in the workflow to ensure compatibility with the kit. NextSeq 500 gds 302664265 GSM2664265 GEO Accession GSM2664265 SRX2902865 GSM2664266 SRP108999 SRS2269423 GSM2664266 OTHER TRANSCRIPTOMIC other The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 240 µl of lysate was incubated with anti-Flag antibody coated magnetic beads and 10000 units of RNase T1 (ThermoFisher scientific, EN0541) to perform digestion of exposed mRNA and ribosome purification simultaneously. 100 µl of Dynabeads protein G coated with 4 µg anti-Flag antibody was used. The lysate-beads-RNase T1 mixture was incubated at 4°C for 6 hours and washed. RNA was extracted from ribosomes bound to the beads by TRIzol Reagent, and the co-precipitant linear acrylamide (ThermoFisher scientific, AM9520) was used to increase the RNA recovery rate. RNA fragments in the range of 30-45 nt was extracted by gel extraction. Library generation for ribosome profiling was performed using NEBNext Small RNA Library Prep Set for Illumina (NEB, E7330S), additional de-phosphorylation and phosphorylation steps were incorporated in the workflow to ensure compatibility with the kit. NextSeq 500 gds 302664266 GSM2664266 GEO Accession GSM2664266 SRX2902866 GSM2664267 SRP108999 SRS2269424 GSM2664267 OTHER TRANSCRIPTOMIC other The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 240 µl of lysate was incubated with anti-Flag antibody coated magnetic beads and 10000 units of RNase T1 (ThermoFisher scientific, EN0541) to perform digestion of exposed mRNA and ribosome purification simultaneously. 100 µl of Dynabeads protein G coated with 4 µg anti-Flag antibody was used. The lysate-beads-RNase T1 mixture was incubated at 4°C for 6 hours and washed. RNA was extracted from ribosomes bound to the beads by TRIzol Reagent, and the co-precipitant linear acrylamide (ThermoFisher scientific, AM9520) was used to increase the RNA recovery rate. RNA fragments in the range of 30-45 nt was extracted by gel extraction. Library generation for ribosome profiling was performed using NEBNext Small RNA Library Prep Set for Illumina (NEB, E7330S), additional de-phosphorylation and phosphorylation steps were incorporated in the workflow to ensure compatibility with the kit. NextSeq 500 gds 302664267 GSM2664267 GEO Accession GSM2664267 SRX2902867 GSM2664268 SRP108999 SRS2269425 GSM2664268 RNA-Seq TRANSCRIPTOMIC cDNA The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 120 µl of the lysate was used for total RNA extraction by TRIzol LS Reagent (ThermoFisher scientific, 10296010) 2.5 µg of total RNA was used for isolation of mRNA with the Dynabeads mRNA DIRECT Purification Kit (ThermoFisher scientific, 61011). The entire isolated mRNA sample was used for library generation with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina sequencing (NEB, E7420S). NextSeq 500 gds 302664268 GSM2664268 GEO Accession GSM2664268 SRX2902868 GSM2664269 SRP108999 SRS2269426 GSM2664269 RNA-Seq TRANSCRIPTOMIC cDNA The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 120 µl of the lysate was used for total RNA extraction by TRIzol LS Reagent (ThermoFisher scientific, 10296010) 2.5 µg of total RNA was used for isolation of mRNA with the Dynabeads mRNA DIRECT Purification Kit (ThermoFisher scientific, 61011). The entire isolated mRNA sample was used for library generation with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina sequencing (NEB, E7420S). NextSeq 500 gds 302664269 GSM2664269 GEO Accession GSM2664269 SRX2902869 GSM2664270 SRP108999 SRS2269427 GSM2664270 RNA-Seq TRANSCRIPTOMIC cDNA The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 120 µl of the lysate was used for total RNA extraction by TRIzol LS Reagent (ThermoFisher scientific, 10296010) 2.5 µg of total RNA was used for isolation of mRNA with the Dynabeads mRNA DIRECT Purification Kit (ThermoFisher scientific, 61011). The entire isolated mRNA sample was used for library generation with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina sequencing (NEB, E7420S). NextSeq 500 gds 302664270 GSM2664270 GEO Accession GSM2664270 SRX2902870 GSM2664271 SRP108999 SRS2269428 GSM2664271 OTHER TRANSCRIPTOMIC other The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 240 µl of lysate was incubated with anti-Flag antibody coated magnetic beads and 10000 units of RNase T1 (ThermoFisher scientific, EN0541) to perform digestion of exposed mRNA and ribosome purification simultaneously. 100 µl of Dynabeads protein G coated with 4 µg anti-Flag antibody was used. The lysate-beads-RNase T1 mixture was incubated at 4°C for 6 hours and washed. RNA was extracted from ribosomes bound to the beads by TRIzol Reagent, and the co-precipitant linear acrylamide (ThermoFisher scientific, AM9520) was used to increase the RNA recovery rate. RNA fragments in the range of 30-45 nt was extracted by gel extraction. Library generation for ribosome profiling was performed using NEBNext Small RNA Library Prep Set for Illumina (NEB, E7330S), additional de-phosphorylation and phosphorylation steps were incorporated in the workflow to ensure compatibility with the kit. NextSeq 500 gds 302664271 GSM2664271 GEO Accession GSM2664271 SRX2902871 GSM2664272 SRP108999 SRS2269429 GSM2664272 OTHER TRANSCRIPTOMIC other The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 240 µl of lysate was incubated with anti-Flag antibody coated magnetic beads and 10000 units of RNase T1 (ThermoFisher scientific, EN0541) to perform digestion of exposed mRNA and ribosome purification simultaneously. 100 µl of Dynabeads protein G coated with 4 µg anti-Flag antibody was used. The lysate-beads-RNase T1 mixture was incubated at 4°C for 6 hours and washed. RNA was extracted from ribosomes bound to the beads by TRIzol Reagent, and the co-precipitant linear acrylamide (ThermoFisher scientific, AM9520) was used to increase the RNA recovery rate. RNA fragments in the range of 30-45 nt was extracted by gel extraction. Library generation for ribosome profiling was performed using NEBNext Small RNA Library Prep Set for Illumina (NEB, E7330S), additional de-phosphorylation and phosphorylation steps were incorporated in the workflow to ensure compatibility with the kit. NextSeq 500 gds 302664272 GSM2664272 GEO Accession GSM2664272 SRX2902872 GSM2664273 SRP108999 SRS2269430 GSM2664273 OTHER TRANSCRIPTOMIC other The frozen tissue was then homogenized in 540 µl lysis solution (10 mM HEPES, PH 7.4, 150 mM KCl, 5 mM MgCl2, 100 µg/ml Cycloheximide) supplemented with 0.5% Triton-X100, 1U/µl ANTI-RNase (ThermoFisher scientific, AM2690) and protease inhibitor (EDTA-free, Sigma, COEDTAF-RO). 240 µl of lysate was incubated with anti-Flag antibody coated magnetic beads and 10000 units of RNase T1 (ThermoFisher scientific, EN0541) to perform digestion of exposed mRNA and ribosome purification simultaneously. 100 µl of Dynabeads protein G coated with 4 µg anti-Flag antibody was used. The lysate-beads-RNase T1 mixture was incubated at 4°C for 6 hours and washed. RNA was extracted from ribosomes bound to the beads by TRIzol Reagent, and the co-precipitant linear acrylamide (ThermoFisher scientific, AM9520) was used to increase the RNA recovery rate. RNA fragments in the range of 30-45 nt was extracted by gel extraction. Library generation for ribosome profiling was performed using NEBNext Small RNA Library Prep Set for Illumina (NEB, E7330S), additional de-phosphorylation and phosphorylation steps were incorporated in the workflow to ensure compatibility with the kit. NextSeq 500 gds 302664273 GSM2664273 GEO Accession GSM2664273