SRP110684 PRJNA392279 GSE100603 High-Throughput Sequencing Reveals Differential Expression of miRNAs in respiratory tree from the sea cucumber Apostichopus japonicus Under Hypoxia Stress The present study identified and characterized miRNAs, which may play a major role in stress resistance. we applied high-throughput sequencing to investigate the alterations of miRNAs expression of sea cucumber under hypoxia stress(DO2_1,DO2_2,DO2_3),slight hypoxia stress(DO4_1,DO4_2,DO4_3) and normal condition(DO8_1,DO8_2,DO8_3). These results will provide a basis for future studies of miRNA regulation in sea cucumbers under hypoxia stress. Overall design: 9 individuals (3 biological replicates×3 sea cucumbers per biological replicate) under hypoxia stress (DO 2mg/L) last for three days were used for our experiments (3 biological replicates: DO2_1,DO2_2,DO2_3). 9 individuals (3 biological replicates×3 sea cucumbers per biological replicate) under slight hypoxia stress (DO 4mg/L) last for three days were used for our experiments (3 biological replicates: DO4_1,DO4_2,DO4_3).9 sea cucumbers under normal condition (DO 8mg/L) (3 biological replicates×3 sea cucumbers per biological replicate) served as the control (3 biological replicates:DO8_1,DO8_2,DO8_3). GSE100603 pubmed 28916650