SRX3038108 Thermophilic UASB reactor SRP113607 PRJNA395859 The experimental setup consisted of two UASB reactors installed in series (R1 and R2), with volumes of 12.1 L (R1) and 5.6 L (R2), in a climate chamber with storage tanks for influent and effluent, diaphragm pump, and fiberglass gasometers (Figure 1). The reactors were constructed with PVC tubes, with phase separators in a Y shape, at a 45° angle to the vertical (Cavalcanti et al., 1999 adapted by Bruno and Oliveira, 2013; Villa-Montoya et al., 2016). The influent and UASB reactors temperatures were maintained in thermophilic range (54 to 56 o C) with a heating and temperature control system (equipped with thermocouple) installed in climate chamber. SRS2386552 SAMN07415631 Thermophilic UASB reactor AMPLICON OTHER PCR Ion Torrent PGM