GSM2719262: L2_1; Drosophila melanogaster; RNA-Seq

SRX3041854 GSM2719262 GSM2719262: L2_1; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389797 GSM2719262 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719262 GSM2719262 GEO Accession GSM2719262 SRX3041855 GSM2719263 GSM2719263: L2_2; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389798 GSM2719263 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719263 GSM2719263 GEO Accession GSM2719263 SRX3041856 GSM2719264 GSM2719264: L2_3; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389799 GSM2719264 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719264 GSM2719264 GEO Accession GSM2719264 SRX3041857 GSM2719265 GSM2719265: L3_2; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389800 GSM2719265 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719265 GSM2719265 GEO Accession GSM2719265 SRX3041858 GSM2719266 GSM2719266: L3_3; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389801 GSM2719266 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719266 GSM2719266 GEO Accession GSM2719266 SRX3041859 GSM2719267 GSM2719267: L3_4; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389802 GSM2719267 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719267 GSM2719267 GEO Accession GSM2719267 SRX3041860 GSM2719268 GSM2719268: 0h_1; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389803 GSM2719268 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719268 GSM2719268 GEO Accession GSM2719268 SRX3041861 GSM2719269 GSM2719269: 0h_2; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389804 GSM2719269 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719269 GSM2719269 GEO Accession GSM2719269 SRX3041862 GSM2719270 GSM2719270: 0h_3; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389805 GSM2719270 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719270 GSM2719270 GEO Accession GSM2719270 SRX3041863 GSM2719271 GSM2719271: 0h_4; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389807 GSM2719271 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719271 GSM2719271 GEO Accession GSM2719271 SRX3041864 GSM2719272 GSM2719272: 3h_1; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389806 GSM2719272 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719272 GSM2719272 GEO Accession GSM2719272 SRX3041865 GSM2719273 GSM2719273: 3h_2; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389808 GSM2719273 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719273 GSM2719273 GEO Accession GSM2719273 SRX3041866 GSM2719274 GSM2719274: 3h_3; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389809 GSM2719274 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719274 GSM2719274 GEO Accession GSM2719274 SRX3041867 GSM2719275 GSM2719275: 6h_1; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389810 GSM2719275 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719275 GSM2719275 GEO Accession GSM2719275 SRX3041868 GSM2719276 GSM2719276: 6h_2; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389811 GSM2719276 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719276 GSM2719276 GEO Accession GSM2719276 SRX3041869 GSM2719277 GSM2719277: 6h_3; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389812 GSM2719277 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719277 GSM2719277 GEO Accession GSM2719277 SRX3041870 GSM2719278 GSM2719278: 6h_4; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389813 GSM2719278 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719278 GSM2719278 GEO Accession GSM2719278 SRX3041871 GSM2719279 GSM2719279: 9h_1; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389814 GSM2719279 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719279 GSM2719279 GEO Accession GSM2719279 SRX3041872 GSM2719280 GSM2719280: 9h_2; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389815 GSM2719280 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719280 GSM2719280 GEO Accession GSM2719280 SRX3041873 GSM2719281 GSM2719281: 9h_3; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389816 GSM2719281 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719281 GSM2719281 GEO Accession GSM2719281 SRX3041874 GSM2719282 GSM2719282: 9h_4; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389817 GSM2719282 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719282 GSM2719282 GEO Accession GSM2719282 SRX3041875 GSM2719283 GSM2719283: 12h_1; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389818 GSM2719283 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719283 GSM2719283 GEO Accession GSM2719283 SRX3041876 GSM2719284 GSM2719284: 12h_2; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389819 GSM2719284 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719284 GSM2719284 GEO Accession GSM2719284 SRX3041877 GSM2719285 GSM2719285: 12h_3; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389820 GSM2719285 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719285 GSM2719285 GEO Accession GSM2719285 SRX3041878 GSM2719286 GSM2719286: 12h_4; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389822 GSM2719286 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719286 GSM2719286 GEO Accession GSM2719286 SRX3041879 GSM2719287 GSM2719287: 15h_1; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389821 GSM2719287 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719287 GSM2719287 GEO Accession GSM2719287 SRX3041880 GSM2719288 GSM2719288: 15h_2; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389823 GSM2719288 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719288 GSM2719288 GEO Accession GSM2719288 SRX3041881 GSM2719289 GSM2719289: 15h_3; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389824 GSM2719289 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719289 GSM2719289 GEO Accession GSM2719289 SRX3041882 GSM2719290 GSM2719290: 15h_4; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389825 GSM2719290 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719290 GSM2719290 GEO Accession GSM2719290 SRX3041883 GSM2719291 GSM2719291: 18h_1; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389826 GSM2719291 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719291 GSM2719291 GEO Accession GSM2719291 SRX3041884 GSM2719292 GSM2719292: 18h_2; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389827 GSM2719292 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719292 GSM2719292 GEO Accession GSM2719292 SRX3041885 GSM2719293 GSM2719293: 18h_3; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389828 GSM2719293 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719293 GSM2719293 GEO Accession GSM2719293 SRX3041886 GSM2719294 GSM2719294: 18h_4; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389829 GSM2719294 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719294 GSM2719294 GEO Accession GSM2719294 SRX3041887 GSM2719295 GSM2719295: 21h_1; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389830 GSM2719295 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719295 GSM2719295 GEO Accession GSM2719295 SRX3041888 GSM2719296 GSM2719296: 21h_2; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389831 GSM2719296 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719296 GSM2719296 GEO Accession GSM2719296 SRX3041889 GSM2719297 GSM2719297: 21h_3; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389832 GSM2719297 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719297 GSM2719297 GEO Accession GSM2719297 SRX3041890 GSM2719298 GSM2719298: 24h_1; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389833 GSM2719298 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719298 GSM2719298 GEO Accession GSM2719298 SRX3041891 GSM2719299 GSM2719299: 24h_2; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389834 GSM2719299 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719299 GSM2719299 GEO Accession GSM2719299 SRX3041892 GSM2719300 GSM2719300: 24h_3; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389835 GSM2719300 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719300 GSM2719300 GEO Accession GSM2719300 SRX3041893 GSM2719301 GSM2719301: 24h_4; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389836 GSM2719301 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719301 GSM2719301 GEO Accession GSM2719301 SRX3041894 GSM2719302 GSM2719302: 27h_1; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389837 GSM2719302 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719302 GSM2719302 GEO Accession GSM2719302 SRX3041895 GSM2719303 GSM2719303: 27h_2; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389839 GSM2719303 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719303 GSM2719303 GEO Accession GSM2719303 SRX3041896 GSM2719304 GSM2719304: 27h_3; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389838 GSM2719304 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719304 GSM2719304 GEO Accession GSM2719304 SRX3041897 GSM2719305 GSM2719305: 27h_4; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389840 GSM2719305 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719305 GSM2719305 GEO Accession GSM2719305 SRX3041898 GSM2719306 GSM2719306: 30h_1; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389841 GSM2719306 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719306 GSM2719306 GEO Accession GSM2719306 SRX3041899 GSM2719307 GSM2719307: 30h_2; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389842 GSM2719307 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719307 GSM2719307 GEO Accession GSM2719307 SRX3041900 GSM2719308 GSM2719308: 30h_3; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389843 GSM2719308 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719308 GSM2719308 GEO Accession GSM2719308 SRX3041901 GSM2719309 GSM2719309: 30h_4; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389844 GSM2719309 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719309 GSM2719309 GEO Accession GSM2719309 SRX3041902 GSM2719310 GSM2719310: Adult_1; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389845 GSM2719310 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719310 GSM2719310 GEO Accession GSM2719310 SRX3041903 GSM2719311 GSM2719311: Adult_2; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389846 GSM2719311 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719311 GSM2719311 GEO Accession GSM2719311 SRX3041904 GSM2719312 GSM2719312: Adult_3; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389847 GSM2719312 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719312 GSM2719312 GEO Accession GSM2719312 SRX3041905 GSM2719313 GSM2719313: Adult_4; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389848 GSM2719313 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719313 GSM2719313 GEO Accession GSM2719313 SRX3041906 GSM2719314 GSM2719314: NP3061_1; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389849 GSM2719314 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719314 GSM2719314 GEO Accession GSM2719314 SRX3041907 GSM2719315 GSM2719315: NP3061_2; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389850 GSM2719315 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719315 GSM2719315 GEO Accession GSM2719315 SRX3041908 GSM2719316 GSM2719316: NP3061_3; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389851 GSM2719316 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719316 GSM2719316 GEO Accession GSM2719316 SRX3041909 GSM2719317 GSM2719317: NP3061_4; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389852 GSM2719317 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719317 GSM2719317 GEO Accession GSM2719317 SRX3041910 GSM2719318 GSM2719318: C155_1; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389853 GSM2719318 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719318 GSM2719318 GEO Accession GSM2719318 SRX3041911 GSM2719319 GSM2719319: C155_2; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389854 GSM2719319 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719319 GSM2719319 GEO Accession GSM2719319 SRX3041912 GSM2719320 GSM2719320: C155_3; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389855 GSM2719320 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719320 GSM2719320 GEO Accession GSM2719320 SRX3041913 GSM2719321 GSM2719321: C155_4; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389856 GSM2719321 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719321 GSM2719321 GEO Accession GSM2719321 SRX3041914 GSM2719322 GSM2719322: ALRM_Adult_1; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389857 GSM2719322 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719322 GSM2719322 GEO Accession GSM2719322 SRX3041915 GSM2719323 GSM2719323: ALRM_Adult_2; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389858 GSM2719323 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719323 GSM2719323 GEO Accession GSM2719323 SRX3041916 GSM2719324 GSM2719324: ALRM_Adult_3; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389859 GSM2719324 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719324 GSM2719324 GEO Accession GSM2719324 SRX3041917 GSM2719325 GSM2719325: ALRM_Adult_4; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389860 GSM2719325 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719325 GSM2719325 GEO Accession GSM2719325 SRX3041918 GSM2719326 GSM2719326: Control_L3_B; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389861 GSM2719326 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719326 GSM2719326 GEO Accession GSM2719326 SRX3041919 GSM2719327 GSM2719327: Control_L3_C; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389863 GSM2719327 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719327 GSM2719327 GEO Accession GSM2719327 SRX3041920 GSM2719328 GSM2719328: Control_0h_A; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389862 GSM2719328 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719328 GSM2719328 GEO Accession GSM2719328 SRX3041921 GSM2719329 GSM2719329: Control_0h_B; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389864 GSM2719329 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719329 GSM2719329 GEO Accession GSM2719329 SRX3041922 GSM2719330 GSM2719330: Control_0h_C; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389865 GSM2719330 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719330 GSM2719330 GEO Accession GSM2719330 SRX3041923 GSM2719331 GSM2719331: Control_6h_A; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389866 GSM2719331 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719331 GSM2719331 GEO Accession GSM2719331 SRX3041924 GSM2719332 GSM2719332: Control_6h_C; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389867 GSM2719332 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719332 GSM2719332 GEO Accession GSM2719332 SRX3041925 GSM2719333 GSM2719333: Control_12h_A; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389868 GSM2719333 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719333 GSM2719333 GEO Accession GSM2719333 SRX3041926 GSM2719334 GSM2719334: Control_12h_B; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389869 GSM2719334 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719334 GSM2719334 GEO Accession GSM2719334 SRX3041927 GSM2719335 GSM2719335: Control_12h_C; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389870 GSM2719335 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719335 GSM2719335 GEO Accession GSM2719335 SRX3041928 GSM2719336 GSM2719336: EcR_L3_A; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389871 GSM2719336 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719336 GSM2719336 GEO Accession GSM2719336 SRX3041929 GSM2719337 GSM2719337: EcR_L3_B; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389872 GSM2719337 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719337 GSM2719337 GEO Accession GSM2719337 SRX3041930 GSM2719338 GSM2719338: EcR_L3_C; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389873 GSM2719338 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719338 GSM2719338 GEO Accession GSM2719338 SRX3041931 GSM2719339 GSM2719339: EcR_0h_A; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389874 GSM2719339 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719339 GSM2719339 GEO Accession GSM2719339 SRX3041932 GSM2719340 GSM2719340: EcR_0h_B; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389875 GSM2719340 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719340 GSM2719340 GEO Accession GSM2719340 SRX3041933 GSM2719341 GSM2719341: EcR_0h_C; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389876 GSM2719341 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719341 GSM2719341 GEO Accession GSM2719341 SRX3041934 GSM2719342 GSM2719342: EcR_6h_A; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389877 GSM2719342 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719342 GSM2719342 GEO Accession GSM2719342 SRX3041935 GSM2719343 GSM2719343: EcR_6h_B; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389878 GSM2719343 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719343 GSM2719343 GEO Accession GSM2719343 SRX3041936 GSM2719344 GSM2719344: EcR_6h_C; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389879 GSM2719344 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719344 GSM2719344 GEO Accession GSM2719344 SRX3041937 GSM2719345 GSM2719345: EcR_12h_A; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389880 GSM2719345 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719345 GSM2719345 GEO Accession GSM2719345 SRX3041938 GSM2719346 GSM2719346: EcR_12h_B; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389882 GSM2719346 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719346 GSM2719346 GEO Accession GSM2719346 SRX3041939 GSM2719347 GSM2719347: E75_L3_A; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389881 GSM2719347 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719347 GSM2719347 GEO Accession GSM2719347 SRX3041940 GSM2719348 GSM2719348: E75_L3_B; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389883 GSM2719348 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719348 GSM2719348 GEO Accession GSM2719348 SRX3041941 GSM2719349 GSM2719349: E75_L3_C; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389884 GSM2719349 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719349 GSM2719349 GEO Accession GSM2719349 SRX3041942 GSM2719350 GSM2719350: E75_0h_A; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389885 GSM2719350 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719350 GSM2719350 GEO Accession GSM2719350 SRX3041943 GSM2719351 GSM2719351: E75_0h_B; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389886 GSM2719351 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719351 GSM2719351 GEO Accession GSM2719351 SRX3041944 GSM2719352 GSM2719352: E75_6h_A; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389887 GSM2719352 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719352 GSM2719352 GEO Accession GSM2719352 SRX3041945 GSM2719353 GSM2719353: E75_6h_B; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389888 GSM2719353 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719353 GSM2719353 GEO Accession GSM2719353 SRX3041946 GSM2719354 GSM2719354: E75_6h_C; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389889 GSM2719354 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719354 GSM2719354 GEO Accession GSM2719354 SRX3041947 GSM2719355 GSM2719355: E75_12h_A; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389891 GSM2719355 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719355 GSM2719355 GEO Accession GSM2719355 SRX3041948 GSM2719356 GSM2719356: E75_12h_C; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389890 GSM2719356 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719356 GSM2719356 GEO Accession GSM2719356 SRX3041949 GSM2719357 GSM2719357: Sox14_L3_A; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389892 GSM2719357 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719357 GSM2719357 GEO Accession GSM2719357 SRX3041950 GSM2719358 GSM2719358: Sox14_L3_B; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389893 GSM2719358 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719358 GSM2719358 GEO Accession GSM2719358 SRX3041951 GSM2719359 GSM2719359: Sox14_L3_C; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389894 GSM2719359 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719359 GSM2719359 GEO Accession GSM2719359 SRX3041952 GSM2719360 GSM2719360: Sox14_0h_A; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389895 GSM2719360 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719360 GSM2719360 GEO Accession GSM2719360 SRX3041953 GSM2719361 GSM2719361: Sox14_0h_B; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389898 GSM2719361 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719361 GSM2719361 GEO Accession GSM2719361 SRX3041954 GSM2719362 GSM2719362: Sox14_0h_C; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389896 GSM2719362 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719362 GSM2719362 GEO Accession GSM2719362 SRX3041955 GSM2719363 GSM2719363: Sox14_6h_A; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389897 GSM2719363 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719363 GSM2719363 GEO Accession GSM2719363 SRX3041956 GSM2719364 GSM2719364: Sox14_6h_B; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389899 GSM2719364 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719364 GSM2719364 GEO Accession GSM2719364 SRX3041957 GSM2719365 GSM2719365: Sox14_6h_C; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389900 GSM2719365 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719365 GSM2719365 GEO Accession GSM2719365 SRX3041958 GSM2719366 GSM2719366: Sox14_12h_A; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389901 GSM2719366 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719366 GSM2719366 GEO Accession GSM2719366 SRX3041959 GSM2719367 GSM2719367: Sox14_12h_B; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389902 GSM2719367 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719367 GSM2719367 GEO Accession GSM2719367 SRX3041960 GSM2719368 GSM2719368: Sox14_12h_C; Drosophila melanogaster; RNA-Seq SRP113673 SRS2389903 GSM2719368 RNA-Seq TRANSCRIPTOMIC cDNA Brains were dissected a cold Ringer’s solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 DsRed+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Library constracted using MARS-seq technique (Jaitin et al, Science 2014). Briefly mRNA was barcoded, converted into cDNA and linearly amplified by T7 in vitro transcription. single end protocol ( samples used read2 to read molecule barcodes and UMI) NextSeq 500 gds 302719368 GSM2719368 GEO Accession GSM2719368