SRX3069147 GSM2735046 SRP114987 PRJNA397451 SRS2413598 SAMN07457646 RNA-Seq TRANSCRIPTOMIC cDNA All the colons were flushed with ice-cold saline to remove feces and opened longitudinally on filter paper. The proximal segment and tumors of the right portion of the colon were removed, then colon epithelial cells were isolated from the remaining tissues using a scraping method as previously described. Briefly, the tissues were scraped using a glass slide on ice and the mucosal scrapings were resuspended in ice cold PBS. The remaining tissue after scraping was snap frozen and stored for further analyses. The epithelial preparations were centrifuged at low-speed (150x g) and washed with ice cold PBS twice to reduce blood cell contamination. Isolated epithelial cells were pelleted, snap frozen with dry ice, then store at -80°C for further analysis. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 302735046 GSM2735046 GEO Accession GSM2735046 SRX3069148 GSM2735047 SRP114987 PRJNA397451 SRS2413600 SAMN07457645 RNA-Seq TRANSCRIPTOMIC cDNA All the colons were flushed with ice-cold saline to remove feces and opened longitudinally on filter paper. The proximal segment and tumors of the right portion of the colon were removed, then colon epithelial cells were isolated from the remaining tissues using a scraping method as previously described. Briefly, the tissues were scraped using a glass slide on ice and the mucosal scrapings were resuspended in ice cold PBS. The remaining tissue after scraping was snap frozen and stored for further analyses. The epithelial preparations were centrifuged at low-speed (150x g) and washed with ice cold PBS twice to reduce blood cell contamination. Isolated epithelial cells were pelleted, snap frozen with dry ice, then store at -80°C for further analysis. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 302735047 GSM2735047 GEO Accession GSM2735047 SRX3069149 GSM2735048 SRP114987 PRJNA397451 SRS2413599 SAMN07457644 RNA-Seq TRANSCRIPTOMIC cDNA All the colons were flushed with ice-cold saline to remove feces and opened longitudinally on filter paper. The proximal segment and tumors of the right portion of the colon were removed, then colon epithelial cells were isolated from the remaining tissues using a scraping method as previously described. Briefly, the tissues were scraped using a glass slide on ice and the mucosal scrapings were resuspended in ice cold PBS. The remaining tissue after scraping was snap frozen and stored for further analyses. The epithelial preparations were centrifuged at low-speed (150x g) and washed with ice cold PBS twice to reduce blood cell contamination. Isolated epithelial cells were pelleted, snap frozen with dry ice, then store at -80°C for further analysis. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 302735048 GSM2735048 GEO Accession GSM2735048 SRX3069150 GSM2735049 SRP114987 PRJNA397451 SRS2413596 SAMN07457695 RNA-Seq TRANSCRIPTOMIC cDNA All the colons were flushed with ice-cold saline to remove feces and opened longitudinally on filter paper. The proximal segment and tumors of the right portion of the colon were removed, then colon epithelial cells were isolated from the remaining tissues using a scraping method as previously described. Briefly, the tissues were scraped using a glass slide on ice and the mucosal scrapings were resuspended in ice cold PBS. The remaining tissue after scraping was snap frozen and stored for further analyses. The epithelial preparations were centrifuged at low-speed (150x g) and washed with ice cold PBS twice to reduce blood cell contamination. Isolated epithelial cells were pelleted, snap frozen with dry ice, then store at -80°C for further analysis. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 302735049 GSM2735049 GEO Accession GSM2735049 SRX3069151 GSM2735050 SRP114987 PRJNA397451 SRS2413597 SAMN07457694 RNA-Seq TRANSCRIPTOMIC cDNA All the colons were flushed with ice-cold saline to remove feces and opened longitudinally on filter paper. The proximal segment and tumors of the right portion of the colon were removed, then colon epithelial cells were isolated from the remaining tissues using a scraping method as previously described. Briefly, the tissues were scraped using a glass slide on ice and the mucosal scrapings were resuspended in ice cold PBS. The remaining tissue after scraping was snap frozen and stored for further analyses. The epithelial preparations were centrifuged at low-speed (150x g) and washed with ice cold PBS twice to reduce blood cell contamination. Isolated epithelial cells were pelleted, snap frozen with dry ice, then store at -80°C for further analysis. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 302735050 GSM2735050 GEO Accession GSM2735050 SRX3069152 GSM2735051 SRP114987 PRJNA397451 SRS2413595 SAMN07457693 RNA-Seq TRANSCRIPTOMIC cDNA All the colons were flushed with ice-cold saline to remove feces and opened longitudinally on filter paper. The proximal segment and tumors of the right portion of the colon were removed, then colon epithelial cells were isolated from the remaining tissues using a scraping method as previously described. Briefly, the tissues were scraped using a glass slide on ice and the mucosal scrapings were resuspended in ice cold PBS. The remaining tissue after scraping was snap frozen and stored for further analyses. The epithelial preparations were centrifuged at low-speed (150x g) and washed with ice cold PBS twice to reduce blood cell contamination. Isolated epithelial cells were pelleted, snap frozen with dry ice, then store at -80°C for further analysis. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 302735051 GSM2735051 GEO Accession GSM2735051 SRX3069153 GSM2735052 SRP114987 PRJNA397451 SRS2413594 SAMN07457692 RNA-Seq TRANSCRIPTOMIC cDNA All the colons were flushed with ice-cold saline to remove feces and opened longitudinally on filter paper. The proximal segment and tumors of the right portion of the colon were removed, then colon epithelial cells were isolated from the remaining tissues using a scraping method as previously described. Briefly, the tissues were scraped using a glass slide on ice and the mucosal scrapings were resuspended in ice cold PBS. The remaining tissue after scraping was snap frozen and stored for further analyses. The epithelial preparations were centrifuged at low-speed (150x g) and washed with ice cold PBS twice to reduce blood cell contamination. Isolated epithelial cells were pelleted, snap frozen with dry ice, then store at -80°C for further analysis. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 302735052 GSM2735052 GEO Accession GSM2735052 SRX3069154 GSM2735053 SRP114987 PRJNA397451 SRS2413592 SAMN07457691 RNA-Seq TRANSCRIPTOMIC cDNA All the colons were flushed with ice-cold saline to remove feces and opened longitudinally on filter paper. The proximal segment and tumors of the right portion of the colon were removed, then colon epithelial cells were isolated from the remaining tissues using a scraping method as previously described. Briefly, the tissues were scraped using a glass slide on ice and the mucosal scrapings were resuspended in ice cold PBS. The remaining tissue after scraping was snap frozen and stored for further analyses. The epithelial preparations were centrifuged at low-speed (150x g) and washed with ice cold PBS twice to reduce blood cell contamination. Isolated epithelial cells were pelleted, snap frozen with dry ice, then store at -80°C for further analysis. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 302735053 GSM2735053 GEO Accession GSM2735053 SRX3069155 GSM2735054 SRP114987 PRJNA397451 SRS2413593 SAMN07457690 RNA-Seq TRANSCRIPTOMIC cDNA All the colons were flushed with ice-cold saline to remove feces and opened longitudinally on filter paper. The proximal segment and tumors of the right portion of the colon were removed, then colon epithelial cells were isolated from the remaining tissues using a scraping method as previously described. Briefly, the tissues were scraped using a glass slide on ice and the mucosal scrapings were resuspended in ice cold PBS. The remaining tissue after scraping was snap frozen and stored for further analyses. The epithelial preparations were centrifuged at low-speed (150x g) and washed with ice cold PBS twice to reduce blood cell contamination. Isolated epithelial cells were pelleted, snap frozen with dry ice, then store at -80°C for further analysis. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 302735054 GSM2735054 GEO Accession GSM2735054 SRX3069156 GSM2735055 SRP114987 PRJNA397451 SRS2413590 SAMN07457689 RNA-Seq TRANSCRIPTOMIC cDNA All the colons were flushed with ice-cold saline to remove feces and opened longitudinally on filter paper. The proximal segment and tumors of the right portion of the colon were removed, then colon epithelial cells were isolated from the remaining tissues using a scraping method as previously described. Briefly, the tissues were scraped using a glass slide on ice and the mucosal scrapings were resuspended in ice cold PBS. The remaining tissue after scraping was snap frozen and stored for further analyses. The epithelial preparations were centrifuged at low-speed (150x g) and washed with ice cold PBS twice to reduce blood cell contamination. Isolated epithelial cells were pelleted, snap frozen with dry ice, then store at -80°C for further analysis. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 302735055 GSM2735055 GEO Accession GSM2735055 SRX3069157 GSM2735056 SRP114987 PRJNA397451 SRS2413589 SAMN07457688 RNA-Seq TRANSCRIPTOMIC cDNA All the colons were flushed with ice-cold saline to remove feces and opened longitudinally on filter paper. The proximal segment and tumors of the right portion of the colon were removed, then colon epithelial cells were isolated from the remaining tissues using a scraping method as previously described. Briefly, the tissues were scraped using a glass slide on ice and the mucosal scrapings were resuspended in ice cold PBS. The remaining tissue after scraping was snap frozen and stored for further analyses. The epithelial preparations were centrifuged at low-speed (150x g) and washed with ice cold PBS twice to reduce blood cell contamination. Isolated epithelial cells were pelleted, snap frozen with dry ice, then store at -80°C for further analysis. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 302735056 GSM2735056 GEO Accession GSM2735056 SRX3069158 GSM2735057 SRP114987 PRJNA397451 SRS2413591 SAMN07457687 RNA-Seq TRANSCRIPTOMIC cDNA All the colons were flushed with ice-cold saline to remove feces and opened longitudinally on filter paper. The proximal segment and tumors of the right portion of the colon were removed, then colon epithelial cells were isolated from the remaining tissues using a scraping method as previously described. Briefly, the tissues were scraped using a glass slide on ice and the mucosal scrapings were resuspended in ice cold PBS. The remaining tissue after scraping was snap frozen and stored for further analyses. The epithelial preparations were centrifuged at low-speed (150x g) and washed with ice cold PBS twice to reduce blood cell contamination. Isolated epithelial cells were pelleted, snap frozen with dry ice, then store at -80°C for further analysis. RNA libraries were prepared for sequencing using standard Illumina protocols NextSeq 500 gds 302735057 GSM2735057 GEO Accession GSM2735057