SRP118600 PRJNA411777 GSE104127 Next Generation Sequencing Analysis of WT and Hectd3–/– BMDMs during F. novicida infection Purpose: The goals of this study are to compare NGS-derived WT and Hectd3–/– transcrip profiling (RNA-seq) in the absence and presence of F. novicida infection Methods: Bone marrow derived macrophages (BMDMs) from WT and Hectd3–/– mice were infected with F .novicida for 8 and 12 hours. mRNA profiles were generated by deep sequencing using Illumina GAIIx. Results: Using an optimized data analysis workflow, we mapped about 80 million sequence reads per sample (around 95% clean reads were successfully mapped) to the mouse genome and identified 22,589 transcripts in the WT and Hectd3–/– BMDMs with or without F. novicida infection. Conclusions: Our study represents the first detailed analysis of HECTD3 deficiency regulated gene expression in response to F. novicida infection generated by RNA-seq technology. Our results demonstrated that the type I IFN signaling induced by F. novicida infection was impaired in the absence of HECTD3. Overall design: mRNA profiles of WT and Hectd3–/– BMDMs with or without F. novicida infection for 8 and 12 h were generated by deep sequencing, using Illumina Hiseq platform. GSE104127 pubmed 29920190