SRX3205375 GSM2790507 SRP118616 SRS2531366 GSM2790507 ATAC-seq GENOMIC other 100,000  TPCs were isolated by FACS and washed once with cold 1x PBS. Cells were pelleted and lysed in 50 μl and spun at 500g for 25min at 4C to collect nuclei. Pelleted nuclei were incubated for 30min at 37 C with 2.5ul of Tn5 transposase (Illumina Cat #FC-121-1030) before transposed DNA fragments were purified with a Qiagen MinElute Kit for ATAC-seq library generation. Illumina HiSeq 2500 gds 302790507 GSM2790507 GEO Accession GSM2790507 SRX3205376 GSM2790508 SRP118616 SRS2531367 GSM2790508 ATAC-seq GENOMIC other 100,000  TPCs were isolated by FACS and washed once with cold 1x PBS. Cells were pelleted and lysed in 50 μl and spun at 500g for 25min at 4C to collect nuclei. Pelleted nuclei were incubated for 30min at 37 C with 2.5ul of Tn5 transposase (Illumina Cat #FC-121-1030) before transposed DNA fragments were purified with a Qiagen MinElute Kit for ATAC-seq library generation. Illumina HiSeq 2500 gds 302790508 GSM2790508 GEO Accession GSM2790508 SRX5282999 GSM3576196 SRP118616 PRJNA411785 SRS4282748 GSM3576196 ATAC-seq GENOMIC other 50,000  tumor-propagating cells (TPCs) were isolated by FACS and washed once with cold 1x PBS. Cells were pelleted and lysed in 50 μl and spun at 500g for 25min at 4C to collect nuclei. Pelleted nuclei were incubated for 30min at 37 C with 2.5ul of Tn5 transposase (Illumina Cat #FC-121-1030) before transposed DNA fragments were purified with a Qiagen MinElute Kit for ATAC-seq library generation. Illumina HiSeq 2500 gds 303576196 GSM3576196 GEO Accession GSM3576196 SRX5283000 GSM3576197 SRP118616 PRJNA411785 SRS4282749 GSM3576197 ATAC-seq GENOMIC other 50,000  tumor-propagating cells (TPCs) were isolated by FACS and washed once with cold 1x PBS. Cells were pelleted and lysed in 50 μl and spun at 500g for 25min at 4C to collect nuclei. Pelleted nuclei were incubated for 30min at 37 C with 2.5ul of Tn5 transposase (Illumina Cat #FC-121-1030) before transposed DNA fragments were purified with a Qiagen MinElute Kit for ATAC-seq library generation. Illumina HiSeq 2500 gds 303576197 GSM3576197 GEO Accession GSM3576197 SRX5283001 GSM3576198 SRP118616 PRJNA411785 SRS4282750 GSM3576198 ATAC-seq GENOMIC other 50,000  tumor-propagating cells (TPCs) were isolated by FACS and washed once with cold 1x PBS. Cells were pelleted and lysed in 50 μl and spun at 500g for 25min at 4C to collect nuclei. Pelleted nuclei were incubated for 30min at 37 C with 2.5ul of Tn5 transposase (Illumina Cat #FC-121-1030) before transposed DNA fragments were purified with a Qiagen MinElute Kit for ATAC-seq library generation. Illumina HiSeq 2500 gds 303576198 GSM3576198 GEO Accession GSM3576198 SRX5283002 GSM3576199 SRP118616 PRJNA411785 SRS4282751 GSM3576199 ATAC-seq GENOMIC other 50,000  tumor-propagating cells (TPCs) were isolated by FACS and washed once with cold 1x PBS. Cells were pelleted and lysed in 50 μl and spun at 500g for 25min at 4C to collect nuclei. Pelleted nuclei were incubated for 30min at 37 C with 2.5ul of Tn5 transposase (Illumina Cat #FC-121-1030) before transposed DNA fragments were purified with a Qiagen MinElute Kit for ATAC-seq library generation. Illumina HiSeq 2500 gds 303576199 GSM3576199 GEO Accession GSM3576199