SRP124146 PRJNA417184 Hsf5 is required for spermatogenesis in zebrafish n the present study we identify Zebrafish Hsf5 and show that it is required for proper spermatogenesis. The hsf5 mutants are male sterile and have malformed sperms. We proved evidences that Hsf5 is required for prophase-1 progression during meiosis. Hsf5 loss leads to misregulation of regulators of cell cycle, DNA damage repair that are required for prophase-1 progression during spermatogenesis. Hsf5 binding is also observed at these genes suggesting Hsf5 is an important upstream regulator for meiotic cells division during spermatogenesis.