SRX3373141 GSM2844742 SRP124590 SRS2670408 GSM2844742 RNA-Seq TRANSCRIPTOMIC cDNA Generation of Arid1aflox/flox mice on the C57BL/6 background was previously described (Gao et al., 2008). Cre-mediated deletion of Arid1a removed exon 8 and introduced a frameshift mutation and a premature stop codon (p.Gly809Hisfs*6; wild type ARID1A protein has 2283 amino acids). Ptenflox/flox mice on the BALB/c background (Strain C;129S4-Ptentm1Hwu/J) were obtained from the Jackson Laboratory (Groszer et al., 2001). Cre-mediated deletion of Pten removed exon 5 and introduced a frameshift mutation (p.Val85Glyfs*14). Arid1aflox/flox;Ptenflox/flox mice were generated by intercrossing these transgenic strains. In order to express Cre recombinase specifically in the mouse uterine epithelium for all subsequent genetic alterations described, we used Pax8-Cre mice which were generated by crossing mice expressing the reverse tetracycline-controlled transactivator (rtTA) under the control of the Pax8 promoter (Pax8-rtTA) with mice expressing Cre recombinase in a tetracycline-dependent manner (TetO-Cre) (Perets et al., 2013). To generate mouse models with Arid1a and Pten individual or combined knockout in uterine epithelium, we crossed Pax8-Cre mice with Arid1aflox/flox mice, Ptenflox/flox mice, and Arid1aflox/flox;Ptenflox/flox mice. Figure S4A summarizes the generation of these genetically engineered mice in which Arid1a and Pten can be conditionally knocked out. All experimental mice were maintained on a mixed genetic background (C57BL/6, BALB/c, and S129). The resultant genotype of each model was confirmed by genomic DNA PCR, using primer sequences described previously (Guan et al., 2014). The wild-type, floxed, and recombined Arid1a generate 669, 845, and 298 bp PCR products, respectively. The predicted PCR products for wild-type, flox, and recombined Pten are 1018, 1100, and 400 bp, respectively. Knockout was initiated by treating mice with doxycycline either through oral gavage (2 mg/mouse/day). All of the animal procedures were approved by the Johns Hopkins University Animal Care Committee. Uteri from 4 iPAD and 4 iPD mice were harvested two weeks after gene knockout (by feeding doxycycline). Hematoxylin-and-eosin stained tissue sections were prepared, and the diagnosis of endometrioid carcinoma from all iPAD mice and EIN from all iPD mice was confirmed by histopathology. Total RNA was isolated from mouse uterine tissues using the Qiagen RNeasy Plus Mini Kit. RNA quality was assessed using the Agilent 2100 Bioanalyzer RNA Nano Chip. RIN values ranged from 5.8 to 7.8. RNA-sequencing was performed by GeneWiz, Inc. on the Illumina HiSeq2500 platform, in a 2x100 bp paired-end high output V4 chemistry configuration. Illumina HiSeq 2500 gds 302844742 GSM2844742 GEO Accession GSM2844742 SRX3373142 GSM2844750 SRP124590 SRS2670406 GSM2844750 RNA-Seq TRANSCRIPTOMIC cDNA Generation of Arid1aflox/flox mice on the C57BL/6 background was previously described (Gao et al., 2008). Cre-mediated deletion of Arid1a removed exon 8 and introduced a frameshift mutation and a premature stop codon (p.Gly809Hisfs*6; wild type ARID1A protein has 2283 amino acids). Ptenflox/flox mice on the BALB/c background (Strain C;129S4-Ptentm1Hwu/J) were obtained from the Jackson Laboratory (Groszer et al., 2001). Cre-mediated deletion of Pten removed exon 5 and introduced a frameshift mutation (p.Val85Glyfs*14). Arid1aflox/flox;Ptenflox/flox mice were generated by intercrossing these transgenic strains. In order to express Cre recombinase specifically in the mouse uterine epithelium for all subsequent genetic alterations described, we used Pax8-Cre mice which were generated by crossing mice expressing the reverse tetracycline-controlled transactivator (rtTA) under the control of the Pax8 promoter (Pax8-rtTA) with mice expressing Cre recombinase in a tetracycline-dependent manner (TetO-Cre) (Perets et al., 2013). To generate mouse models with Arid1a and Pten individual or combined knockout in uterine epithelium, we crossed Pax8-Cre mice with Arid1aflox/flox mice, Ptenflox/flox mice, and Arid1aflox/flox;Ptenflox/flox mice. Figure S4A summarizes the generation of these genetically engineered mice in which Arid1a and Pten can be conditionally knocked out. All experimental mice were maintained on a mixed genetic background (C57BL/6, BALB/c, and S129). The resultant genotype of each model was confirmed by genomic DNA PCR, using primer sequences described previously (Guan et al., 2014). The wild-type, floxed, and recombined Arid1a generate 669, 845, and 298 bp PCR products, respectively. The predicted PCR products for wild-type, flox, and recombined Pten are 1018, 1100, and 400 bp, respectively. Knockout was initiated by treating mice with doxycycline either through oral gavage (2 mg/mouse/day). All of the animal procedures were approved by the Johns Hopkins University Animal Care Committee. Uteri from 4 iPAD and 4 iPD mice were harvested two weeks after gene knockout (by feeding doxycycline). Hematoxylin-and-eosin stained tissue sections were prepared, and the diagnosis of endometrioid carcinoma from all iPAD mice and EIN from all iPD mice was confirmed by histopathology. Total RNA was isolated from mouse uterine tissues using the Qiagen RNeasy Plus Mini Kit. RNA quality was assessed using the Agilent 2100 Bioanalyzer RNA Nano Chip. RIN values ranged from 5.8 to 7.8. RNA-sequencing was performed by GeneWiz, Inc. on the Illumina HiSeq2500 platform, in a 2x100 bp paired-end high output V4 chemistry configuration. Illumina HiSeq 2500 gds 302844750 GSM2844750 GEO Accession GSM2844750 SRX3373143 GSM2844752 SRP124590 SRS2670407 GSM2844752 RNA-Seq TRANSCRIPTOMIC cDNA Generation of Arid1aflox/flox mice on the C57BL/6 background was previously described (Gao et al., 2008). Cre-mediated deletion of Arid1a removed exon 8 and introduced a frameshift mutation and a premature stop codon (p.Gly809Hisfs*6; wild type ARID1A protein has 2283 amino acids). Ptenflox/flox mice on the BALB/c background (Strain C;129S4-Ptentm1Hwu/J) were obtained from the Jackson Laboratory (Groszer et al., 2001). Cre-mediated deletion of Pten removed exon 5 and introduced a frameshift mutation (p.Val85Glyfs*14). Arid1aflox/flox;Ptenflox/flox mice were generated by intercrossing these transgenic strains. In order to express Cre recombinase specifically in the mouse uterine epithelium for all subsequent genetic alterations described, we used Pax8-Cre mice which were generated by crossing mice expressing the reverse tetracycline-controlled transactivator (rtTA) under the control of the Pax8 promoter (Pax8-rtTA) with mice expressing Cre recombinase in a tetracycline-dependent manner (TetO-Cre) (Perets et al., 2013). To generate mouse models with Arid1a and Pten individual or combined knockout in uterine epithelium, we crossed Pax8-Cre mice with Arid1aflox/flox mice, Ptenflox/flox mice, and Arid1aflox/flox;Ptenflox/flox mice. Figure S4A summarizes the generation of these genetically engineered mice in which Arid1a and Pten can be conditionally knocked out. All experimental mice were maintained on a mixed genetic background (C57BL/6, BALB/c, and S129). The resultant genotype of each model was confirmed by genomic DNA PCR, using primer sequences described previously (Guan et al., 2014). The wild-type, floxed, and recombined Arid1a generate 669, 845, and 298 bp PCR products, respectively. The predicted PCR products for wild-type, flox, and recombined Pten are 1018, 1100, and 400 bp, respectively. Knockout was initiated by treating mice with doxycycline either through oral gavage (2 mg/mouse/day). All of the animal procedures were approved by the Johns Hopkins University Animal Care Committee. Uteri from 4 iPAD and 4 iPD mice were harvested two weeks after gene knockout (by feeding doxycycline). Hematoxylin-and-eosin stained tissue sections were prepared, and the diagnosis of endometrioid carcinoma from all iPAD mice and EIN from all iPD mice was confirmed by histopathology. Total RNA was isolated from mouse uterine tissues using the Qiagen RNeasy Plus Mini Kit. RNA quality was assessed using the Agilent 2100 Bioanalyzer RNA Nano Chip. RIN values ranged from 5.8 to 7.8. RNA-sequencing was performed by GeneWiz, Inc. on the Illumina HiSeq2500 platform, in a 2x100 bp paired-end high output V4 chemistry configuration. Illumina HiSeq 2500 gds 302844752 GSM2844752 GEO Accession GSM2844752 SRX3373144 GSM2844754 SRP124590 SRS2670409 GSM2844754 RNA-Seq TRANSCRIPTOMIC cDNA Generation of Arid1aflox/flox mice on the C57BL/6 background was previously described (Gao et al., 2008). Cre-mediated deletion of Arid1a removed exon 8 and introduced a frameshift mutation and a premature stop codon (p.Gly809Hisfs*6; wild type ARID1A protein has 2283 amino acids). Ptenflox/flox mice on the BALB/c background (Strain C;129S4-Ptentm1Hwu/J) were obtained from the Jackson Laboratory (Groszer et al., 2001). Cre-mediated deletion of Pten removed exon 5 and introduced a frameshift mutation (p.Val85Glyfs*14). Arid1aflox/flox;Ptenflox/flox mice were generated by intercrossing these transgenic strains. In order to express Cre recombinase specifically in the mouse uterine epithelium for all subsequent genetic alterations described, we used Pax8-Cre mice which were generated by crossing mice expressing the reverse tetracycline-controlled transactivator (rtTA) under the control of the Pax8 promoter (Pax8-rtTA) with mice expressing Cre recombinase in a tetracycline-dependent manner (TetO-Cre) (Perets et al., 2013). To generate mouse models with Arid1a and Pten individual or combined knockout in uterine epithelium, we crossed Pax8-Cre mice with Arid1aflox/flox mice, Ptenflox/flox mice, and Arid1aflox/flox;Ptenflox/flox mice. Figure S4A summarizes the generation of these genetically engineered mice in which Arid1a and Pten can be conditionally knocked out. All experimental mice were maintained on a mixed genetic background (C57BL/6, BALB/c, and S129). The resultant genotype of each model was confirmed by genomic DNA PCR, using primer sequences described previously (Guan et al., 2014). The wild-type, floxed, and recombined Arid1a generate 669, 845, and 298 bp PCR products, respectively. The predicted PCR products for wild-type, flox, and recombined Pten are 1018, 1100, and 400 bp, respectively. Knockout was initiated by treating mice with doxycycline either through oral gavage (2 mg/mouse/day). All of the animal procedures were approved by the Johns Hopkins University Animal Care Committee. Uteri from 4 iPAD and 4 iPD mice were harvested two weeks after gene knockout (by feeding doxycycline). Hematoxylin-and-eosin stained tissue sections were prepared, and the diagnosis of endometrioid carcinoma from all iPAD mice and EIN from all iPD mice was confirmed by histopathology. Total RNA was isolated from mouse uterine tissues using the Qiagen RNeasy Plus Mini Kit. RNA quality was assessed using the Agilent 2100 Bioanalyzer RNA Nano Chip. RIN values ranged from 5.8 to 7.8. RNA-sequencing was performed by GeneWiz, Inc. on the Illumina HiSeq2500 platform, in a 2x100 bp paired-end high output V4 chemistry configuration. Illumina HiSeq 2500 gds 302844754 GSM2844754 GEO Accession GSM2844754 SRX3373145 GSM2844756 SRP124590 SRS2670501 GSM2844756 RNA-Seq TRANSCRIPTOMIC cDNA Generation of Arid1aflox/flox mice on the C57BL/6 background was previously described (Gao et al., 2008). Cre-mediated deletion of Arid1a removed exon 8 and introduced a frameshift mutation and a premature stop codon (p.Gly809Hisfs*6; wild type ARID1A protein has 2283 amino acids). Ptenflox/flox mice on the BALB/c background (Strain C;129S4-Ptentm1Hwu/J) were obtained from the Jackson Laboratory (Groszer et al., 2001). Cre-mediated deletion of Pten removed exon 5 and introduced a frameshift mutation (p.Val85Glyfs*14). Arid1aflox/flox;Ptenflox/flox mice were generated by intercrossing these transgenic strains. In order to express Cre recombinase specifically in the mouse uterine epithelium for all subsequent genetic alterations described, we used Pax8-Cre mice which were generated by crossing mice expressing the reverse tetracycline-controlled transactivator (rtTA) under the control of the Pax8 promoter (Pax8-rtTA) with mice expressing Cre recombinase in a tetracycline-dependent manner (TetO-Cre) (Perets et al., 2013). To generate mouse models with Arid1a and Pten individual or combined knockout in uterine epithelium, we crossed Pax8-Cre mice with Arid1aflox/flox mice, Ptenflox/flox mice, and Arid1aflox/flox;Ptenflox/flox mice. Figure S4A summarizes the generation of these genetically engineered mice in which Arid1a and Pten can be conditionally knocked out. All experimental mice were maintained on a mixed genetic background (C57BL/6, BALB/c, and S129). The resultant genotype of each model was confirmed by genomic DNA PCR, using primer sequences described previously (Guan et al., 2014). The wild-type, floxed, and recombined Arid1a generate 669, 845, and 298 bp PCR products, respectively. The predicted PCR products for wild-type, flox, and recombined Pten are 1018, 1100, and 400 bp, respectively. Knockout was initiated by treating mice with doxycycline either through oral gavage (2 mg/mouse/day). All of the animal procedures were approved by the Johns Hopkins University Animal Care Committee. Uteri from 4 iPAD and 4 iPD mice were harvested two weeks after gene knockout (by feeding doxycycline). Hematoxylin-and-eosin stained tissue sections were prepared, and the diagnosis of endometrioid carcinoma from all iPAD mice and EIN from all iPD mice was confirmed by histopathology. Total RNA was isolated from mouse uterine tissues using the Qiagen RNeasy Plus Mini Kit. RNA quality was assessed using the Agilent 2100 Bioanalyzer RNA Nano Chip. RIN values ranged from 5.8 to 7.8. RNA-sequencing was performed by GeneWiz, Inc. on the Illumina HiSeq2500 platform, in a 2x100 bp paired-end high output V4 chemistry configuration. Illumina HiSeq 2500 gds 302844756 GSM2844756 GEO Accession GSM2844756 SRX3373146 GSM2844758 SRP124590 SRS2670410 GSM2844758 RNA-Seq TRANSCRIPTOMIC cDNA Generation of Arid1aflox/flox mice on the C57BL/6 background was previously described (Gao et al., 2008). Cre-mediated deletion of Arid1a removed exon 8 and introduced a frameshift mutation and a premature stop codon (p.Gly809Hisfs*6; wild type ARID1A protein has 2283 amino acids). Ptenflox/flox mice on the BALB/c background (Strain C;129S4-Ptentm1Hwu/J) were obtained from the Jackson Laboratory (Groszer et al., 2001). Cre-mediated deletion of Pten removed exon 5 and introduced a frameshift mutation (p.Val85Glyfs*14). Arid1aflox/flox;Ptenflox/flox mice were generated by intercrossing these transgenic strains. In order to express Cre recombinase specifically in the mouse uterine epithelium for all subsequent genetic alterations described, we used Pax8-Cre mice which were generated by crossing mice expressing the reverse tetracycline-controlled transactivator (rtTA) under the control of the Pax8 promoter (Pax8-rtTA) with mice expressing Cre recombinase in a tetracycline-dependent manner (TetO-Cre) (Perets et al., 2013). To generate mouse models with Arid1a and Pten individual or combined knockout in uterine epithelium, we crossed Pax8-Cre mice with Arid1aflox/flox mice, Ptenflox/flox mice, and Arid1aflox/flox;Ptenflox/flox mice. Figure S4A summarizes the generation of these genetically engineered mice in which Arid1a and Pten can be conditionally knocked out. All experimental mice were maintained on a mixed genetic background (C57BL/6, BALB/c, and S129). The resultant genotype of each model was confirmed by genomic DNA PCR, using primer sequences described previously (Guan et al., 2014). The wild-type, floxed, and recombined Arid1a generate 669, 845, and 298 bp PCR products, respectively. The predicted PCR products for wild-type, flox, and recombined Pten are 1018, 1100, and 400 bp, respectively. Knockout was initiated by treating mice with doxycycline either through oral gavage (2 mg/mouse/day). All of the animal procedures were approved by the Johns Hopkins University Animal Care Committee. Uteri from 4 iPAD and 4 iPD mice were harvested two weeks after gene knockout (by feeding doxycycline). Hematoxylin-and-eosin stained tissue sections were prepared, and the diagnosis of endometrioid carcinoma from all iPAD mice and EIN from all iPD mice was confirmed by histopathology. Total RNA was isolated from mouse uterine tissues using the Qiagen RNeasy Plus Mini Kit. RNA quality was assessed using the Agilent 2100 Bioanalyzer RNA Nano Chip. RIN values ranged from 5.8 to 7.8. RNA-sequencing was performed by GeneWiz, Inc. on the Illumina HiSeq2500 platform, in a 2x100 bp paired-end high output V4 chemistry configuration. Illumina HiSeq 2500 gds 302844758 GSM2844758 GEO Accession GSM2844758 SRX3373147 GSM2844761 SRP124590 SRS2670411 GSM2844761 RNA-Seq TRANSCRIPTOMIC cDNA Generation of Arid1aflox/flox mice on the C57BL/6 background was previously described (Gao et al., 2008). Cre-mediated deletion of Arid1a removed exon 8 and introduced a frameshift mutation and a premature stop codon (p.Gly809Hisfs*6; wild type ARID1A protein has 2283 amino acids). Ptenflox/flox mice on the BALB/c background (Strain C;129S4-Ptentm1Hwu/J) were obtained from the Jackson Laboratory (Groszer et al., 2001). Cre-mediated deletion of Pten removed exon 5 and introduced a frameshift mutation (p.Val85Glyfs*14). Arid1aflox/flox;Ptenflox/flox mice were generated by intercrossing these transgenic strains. In order to express Cre recombinase specifically in the mouse uterine epithelium for all subsequent genetic alterations described, we used Pax8-Cre mice which were generated by crossing mice expressing the reverse tetracycline-controlled transactivator (rtTA) under the control of the Pax8 promoter (Pax8-rtTA) with mice expressing Cre recombinase in a tetracycline-dependent manner (TetO-Cre) (Perets et al., 2013). To generate mouse models with Arid1a and Pten individual or combined knockout in uterine epithelium, we crossed Pax8-Cre mice with Arid1aflox/flox mice, Ptenflox/flox mice, and Arid1aflox/flox;Ptenflox/flox mice. Figure S4A summarizes the generation of these genetically engineered mice in which Arid1a and Pten can be conditionally knocked out. All experimental mice were maintained on a mixed genetic background (C57BL/6, BALB/c, and S129). The resultant genotype of each model was confirmed by genomic DNA PCR, using primer sequences described previously (Guan et al., 2014). The wild-type, floxed, and recombined Arid1a generate 669, 845, and 298 bp PCR products, respectively. The predicted PCR products for wild-type, flox, and recombined Pten are 1018, 1100, and 400 bp, respectively. Knockout was initiated by treating mice with doxycycline either through oral gavage (2 mg/mouse/day). All of the animal procedures were approved by the Johns Hopkins University Animal Care Committee. Uteri from 4 iPAD and 4 iPD mice were harvested two weeks after gene knockout (by feeding doxycycline). Hematoxylin-and-eosin stained tissue sections were prepared, and the diagnosis of endometrioid carcinoma from all iPAD mice and EIN from all iPD mice was confirmed by histopathology. Total RNA was isolated from mouse uterine tissues using the Qiagen RNeasy Plus Mini Kit. RNA quality was assessed using the Agilent 2100 Bioanalyzer RNA Nano Chip. RIN values ranged from 5.8 to 7.8. RNA-sequencing was performed by GeneWiz, Inc. on the Illumina HiSeq2500 platform, in a 2x100 bp paired-end high output V4 chemistry configuration. Illumina HiSeq 2500 gds 302844761 GSM2844761 GEO Accession GSM2844761 SRX3373148 GSM2844763 SRP124590 SRS2670412 GSM2844763 RNA-Seq TRANSCRIPTOMIC cDNA Generation of Arid1aflox/flox mice on the C57BL/6 background was previously described (Gao et al., 2008). Cre-mediated deletion of Arid1a removed exon 8 and introduced a frameshift mutation and a premature stop codon (p.Gly809Hisfs*6; wild type ARID1A protein has 2283 amino acids). Ptenflox/flox mice on the BALB/c background (Strain C;129S4-Ptentm1Hwu/J) were obtained from the Jackson Laboratory (Groszer et al., 2001). Cre-mediated deletion of Pten removed exon 5 and introduced a frameshift mutation (p.Val85Glyfs*14). Arid1aflox/flox;Ptenflox/flox mice were generated by intercrossing these transgenic strains. In order to express Cre recombinase specifically in the mouse uterine epithelium for all subsequent genetic alterations described, we used Pax8-Cre mice which were generated by crossing mice expressing the reverse tetracycline-controlled transactivator (rtTA) under the control of the Pax8 promoter (Pax8-rtTA) with mice expressing Cre recombinase in a tetracycline-dependent manner (TetO-Cre) (Perets et al., 2013). To generate mouse models with Arid1a and Pten individual or combined knockout in uterine epithelium, we crossed Pax8-Cre mice with Arid1aflox/flox mice, Ptenflox/flox mice, and Arid1aflox/flox;Ptenflox/flox mice. Figure S4A summarizes the generation of these genetically engineered mice in which Arid1a and Pten can be conditionally knocked out. All experimental mice were maintained on a mixed genetic background (C57BL/6, BALB/c, and S129). The resultant genotype of each model was confirmed by genomic DNA PCR, using primer sequences described previously (Guan et al., 2014). The wild-type, floxed, and recombined Arid1a generate 669, 845, and 298 bp PCR products, respectively. The predicted PCR products for wild-type, flox, and recombined Pten are 1018, 1100, and 400 bp, respectively. Knockout was initiated by treating mice with doxycycline either through oral gavage (2 mg/mouse/day). All of the animal procedures were approved by the Johns Hopkins University Animal Care Committee. Uteri from 4 iPAD and 4 iPD mice were harvested two weeks after gene knockout (by feeding doxycycline). Hematoxylin-and-eosin stained tissue sections were prepared, and the diagnosis of endometrioid carcinoma from all iPAD mice and EIN from all iPD mice was confirmed by histopathology. Total RNA was isolated from mouse uterine tissues using the Qiagen RNeasy Plus Mini Kit. RNA quality was assessed using the Agilent 2100 Bioanalyzer RNA Nano Chip. RIN values ranged from 5.8 to 7.8. RNA-sequencing was performed by GeneWiz, Inc. on the Illumina HiSeq2500 platform, in a 2x100 bp paired-end high output V4 chemistry configuration. Illumina HiSeq 2500 gds 302844763 GSM2844763 GEO Accession GSM2844763 SRX5195761 GSM3537275 SRP124590 PRJNA417568 SRS4201623 GSM3537275 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from iPAD and iPD mouse uterine tissues using the Qiagen RNeasy Plus Mini Kit. Uteri from iAD mice were harvested and subjected to epithelial cells isolation using Multi Tissue Dissociation Kit 1, CD45 MicroBeads, and CD326 MicroBeads (all from Miltenyi Biotech) according to manufacturer protocols. Total RNA from the isolated iAD mice uterine epithelial cells were extracted using the Qiagen RNeasy Plus Micro Kit. RNA quality was assessed using the Agilent 2100 Bioanalyzer RNA Nano Chip. RIN values ranged from 5.8 to 7.8 for iPD and iPAD mice uteri, and 7.0 to 9.4 for iAD mice uterine epithelial cells. RNA-sequencing was performed by GeneWiz, Inc. on the Illumina HiSeq2500 platform, in a 2x150 bp paired-end high output V4 chemistry configuration. Illumina HiSeq 2500 gds 303537275 GSM3537275 GEO Accession GSM3537275 SRX5195762 GSM3537276 SRP124590 PRJNA417568 SRS4201624 GSM3537276 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was isolated from iPAD and iPD mouse uterine tissues using the Qiagen RNeasy Plus Mini Kit. Uteri from iAD mice were harvested and subjected to epithelial cells isolation using Multi Tissue Dissociation Kit 1, CD45 MicroBeads, and CD326 MicroBeads (all from Miltenyi Biotech) according to manufacturer protocols. Total RNA from the isolated iAD mice uterine epithelial cells were extracted using the Qiagen RNeasy Plus Micro Kit. RNA quality was assessed using the Agilent 2100 Bioanalyzer RNA Nano Chip. RIN values ranged from 5.8 to 7.8 for iPD and iPAD mice uteri, and 7.0 to 9.4 for iAD mice uterine epithelial cells. RNA-sequencing was performed by GeneWiz, Inc. on the Illumina HiSeq2500 platform, in a 2x150 bp paired-end high output V4 chemistry configuration. Illumina HiSeq 2500 gds 303537276 GSM3537276 GEO Accession GSM3537276