SRX3374302 GSM2845288 GSM2845288: embryo_A+_uninjected; Danio rerio; OTHER SRP124609 SRS2671533 GSM2845288 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina HiSeq 2500 gds 302845288 GEO Accession GSM2845288 SRX3374303 GSM2845289 GSM2845289: embryo_A+_1h; Danio rerio; OTHER SRP124609 SRS2671532 GSM2845289 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina HiSeq 2500 gds 302845289 GEO Accession GSM2845289 SRX3374304 GSM2845290 GSM2845290: embryo_A+_2h; Danio rerio; OTHER SRP124609 SRS2671534 GSM2845290 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina HiSeq 2500 gds 302845290 GEO Accession GSM2845290 SRX3374305 GSM2845291 GSM2845291: embryo_A+_3h; Danio rerio; OTHER SRP124609 SRS2671535 GSM2845291 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina HiSeq 2500 gds 302845291 GEO Accession GSM2845291 SRX3374306 GSM2845292 GSM2845292: embryo_A+_4h; Danio rerio; OTHER SRP124609 SRS2671536 GSM2845292 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina HiSeq 2500 gds 302845292 GEO Accession GSM2845292 SRX3374307 GSM2845293 GSM2845293: embryo_A+_5h; Danio rerio; OTHER SRP124609 SRS2671539 GSM2845293 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina HiSeq 2500 gds 302845293 GEO Accession GSM2845293 SRX3374308 GSM2845294 GSM2845294: embryo_A+_6h; Danio rerio; OTHER SRP124609 SRS2671537 GSM2845294 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina HiSeq 2500 gds 302845294 GEO Accession GSM2845294 SRX3374309 GSM2845295 GSM2845295: embryo_A+_7h; Danio rerio; OTHER SRP124609 SRS2671538 GSM2845295 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina HiSeq 2500 gds 302845295 GEO Accession GSM2845295 SRX3374310 GSM2845296 GSM2845296: embryo_A+_8h; Danio rerio; OTHER SRP124609 SRS2671540 GSM2845296 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina HiSeq 2500 gds 302845296 GEO Accession GSM2845296 SRX3374311 GSM2845297 GSM2845297: embryo_A+_10h; Danio rerio; OTHER SRP124609 SRS2671597 GSM2845297 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina HiSeq 2500 gds 302845297 GEO Accession GSM2845297 SRX3374312 GSM2845298 GSM2845298: embryo_A-_uninjected; Danio rerio; OTHER SRP124609 SRS2671541 GSM2845298 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina HiSeq 2500 gds 302845298 GEO Accession GSM2845298 SRX3374313 GSM2845299 GSM2845299: embryo_A-_1h; Danio rerio; OTHER SRP124609 SRS2671542 GSM2845299 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina HiSeq 2500 gds 302845299 GEO Accession GSM2845299 SRX3374314 GSM2845300 GSM2845300: embryo_A-_2h; Danio rerio; OTHER SRP124609 SRS2671543 GSM2845300 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina HiSeq 2500 gds 302845300 GEO Accession GSM2845300 SRX3374315 GSM2845301 GSM2845301: embryo_A-_3h; Danio rerio; OTHER SRP124609 SRS2671544 GSM2845301 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina HiSeq 2500 gds 302845301 GEO Accession GSM2845301 SRX3374316 GSM2845302 GSM2845302: embryo_A-_4h; Danio rerio; OTHER SRP124609 SRS2671545 GSM2845302 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina HiSeq 2500 gds 302845302 GEO Accession GSM2845302 SRX3374317 GSM2845303 GSM2845303: embryo_A-_5h; Danio rerio; OTHER SRP124609 SRS2671546 GSM2845303 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina HiSeq 2500 gds 302845303 GEO Accession GSM2845303 SRX3374318 GSM2845304 GSM2845304: embryo_A-_6h; Danio rerio; OTHER SRP124609 SRS2671547 GSM2845304 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina HiSeq 2500 gds 302845304 GEO Accession GSM2845304 SRX3374319 GSM2845305 GSM2845305: embryo_A-_7h; Danio rerio; OTHER SRP124609 SRS2671549 GSM2845305 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina HiSeq 2500 gds 302845305 GEO Accession GSM2845305 SRX3374320 GSM2845306 GSM2845306: embryo_A-_8h; Danio rerio; OTHER SRP124609 SRS2671550 GSM2845306 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina HiSeq 2500 gds 302845306 GEO Accession GSM2845306 SRX3374321 GSM2845307 GSM2845307: embryo_A-_10h; Danio rerio; OTHER SRP124609 SRS2671548 GSM2845307 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina HiSeq 2500 gds 302845307 GEO Accession GSM2845307 SRX3374322 GSM2845308 GSM2845308: biorep_A+_uninjected; Danio rerio; OTHER SRP124609 SRS2671551 GSM2845308 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845308 GEO Accession GSM2845308 SRX3374323 GSM2845309 GSM2845309: biorep_A+_2h.1; Danio rerio; OTHER SRP124609 SRS2671552 GSM2845309 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845309 GEO Accession GSM2845309 SRX3374324 GSM2845310 GSM2845310: biorep_A+_4h.1; Danio rerio; OTHER SRP124609 SRS2671598 GSM2845310 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845310 GEO Accession GSM2845310 SRX3374325 GSM2845311 GSM2845311: biorep_A+_6h.1; Danio rerio; OTHER SRP124609 SRS2671553 GSM2845311 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845311 GEO Accession GSM2845311 SRX3374326 GSM2845312 GSM2845312: biorep_A+_8h.1; Danio rerio; OTHER SRP124609 SRS2671554 GSM2845312 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845312 GEO Accession GSM2845312 SRX3374327 GSM2845313 GSM2845313: biorep_A+_10h.1; Danio rerio; OTHER SRP124609 SRS2671555 GSM2845313 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845313 GEO Accession GSM2845313 SRX3374328 GSM2845314 GSM2845314: biorep_A+_1h.2; Danio rerio; OTHER SRP124609 SRS2671556 GSM2845314 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845314 GEO Accession GSM2845314 SRX3374329 GSM2845315 GSM2845315: biorep_A+_4h.2; Danio rerio; OTHER SRP124609 SRS2671557 GSM2845315 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845315 GEO Accession GSM2845315 SRX3374330 GSM2845316 GSM2845316: biorep_A+_6h.2; Danio rerio; OTHER SRP124609 SRS2671558 GSM2845316 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845316 GEO Accession GSM2845316 SRX3374331 GSM2845317 GSM2845317: biorep_A+_8h.2; Danio rerio; OTHER SRP124609 SRS2671559 GSM2845317 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845317 GEO Accession GSM2845317 SRX3374332 GSM2845318 GSM2845318: biorep_A+_10h.2; Danio rerio; OTHER SRP124609 SRS2671561 GSM2845318 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845318 GEO Accession GSM2845318 SRX3374333 GSM2845319 GSM2845319: techrep_A+_uninjected.1; Danio rerio; OTHER SRP124609 SRS2671560 GSM2845319 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845319 GEO Accession GSM2845319 SRX3374334 GSM2845320 GSM2845320: techrep_A+_1h.1; Danio rerio; OTHER SRP124609 SRS2671562 GSM2845320 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845320 GEO Accession GSM2845320 SRX3374335 GSM2845321 GSM2845321: techrep_A+_2h.1; Danio rerio; OTHER SRP124609 SRS2671563 GSM2845321 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845321 GEO Accession GSM2845321 SRX3374336 GSM2845322 GSM2845322: techrep_A+_3h.1; Danio rerio; OTHER SRP124609 SRS2671564 GSM2845322 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845322 GEO Accession GSM2845322 SRX3374337 GSM2845323 GSM2845323: techrep_A+_4h.1; Danio rerio; OTHER SRP124609 SRS2671565 GSM2845323 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845323 GEO Accession GSM2845323 SRX3374338 GSM2845324 GSM2845324: techrep_A+_5h.1; Danio rerio; OTHER SRP124609 SRS2671566 GSM2845324 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845324 GEO Accession GSM2845324 SRX3374339 GSM2845325 GSM2845325: techrep_A+_6h.1.1; Danio rerio; OTHER SRP124609 SRS2671567 GSM2845325 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845325 GEO Accession GSM2845325 SRX3374340 GSM2845326 GSM2845326: techrep_A+_6h.1.2; Danio rerio; OTHER SRP124609 SRS2671568 GSM2845326 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845326 GEO Accession GSM2845326 SRX3374341 GSM2845327 GSM2845327: techrep_A+_7h.1; Danio rerio; OTHER SRP124609 SRS2671569 GSM2845327 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845327 GEO Accession GSM2845327 SRX3374342 GSM2845328 GSM2845328: techrep_A+_8h.1; Danio rerio; OTHER SRP124609 SRS2671570 GSM2845328 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845328 GEO Accession GSM2845328 SRX3374343 GSM2845329 GSM2845329: techrep_A+_9h.1; Danio rerio; OTHER SRP124609 SRS2671572 GSM2845329 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845329 GEO Accession GSM2845329 SRX3374344 GSM2845330 GSM2845330: techrep_A+_10h.1; Danio rerio; OTHER SRP124609 SRS2671571 GSM2845330 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845330 GEO Accession GSM2845330 SRX3374345 GSM2845331 GSM2845331: techrep_A+_uninjected.2; Danio rerio; OTHER SRP124609 SRS2671574 GSM2845331 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845331 GEO Accession GSM2845331 SRX3374346 GSM2845332 GSM2845332: techrep_A+_1h.2; Danio rerio; OTHER SRP124609 SRS2671575 GSM2845332 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845332 GEO Accession GSM2845332 SRX3374347 GSM2845333 GSM2845333: techrep_A+_2h.2; Danio rerio; OTHER SRP124609 SRS2671573 GSM2845333 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845333 GEO Accession GSM2845333 SRX3374348 GSM2845334 GSM2845334: techrep_A+_3h.2; Danio rerio; OTHER SRP124609 SRS2671576 GSM2845334 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845334 GEO Accession GSM2845334 SRX3374349 GSM2845335 GSM2845335: techrep_A+_4h.2; Danio rerio; OTHER SRP124609 SRS2671578 GSM2845335 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845335 GEO Accession GSM2845335 SRX3374350 GSM2845336 GSM2845336: techrep_A+_5h.2; Danio rerio; OTHER SRP124609 SRS2671577 GSM2845336 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845336 GEO Accession GSM2845336 SRX3374351 GSM2845337 GSM2845337: techrep_A+_6h.2.1; Danio rerio; OTHER SRP124609 SRS2671599 GSM2845337 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845337 GEO Accession GSM2845337 SRX3374352 GSM2845338 GSM2845338: techrep_A+_6h.2.2; Danio rerio; OTHER SRP124609 SRS2671579 GSM2845338 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845338 GEO Accession GSM2845338 SRX3374353 GSM2845339 GSM2845339: techrep_A+_7h.2; Danio rerio; OTHER SRP124609 SRS2671581 GSM2845339 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845339 GEO Accession GSM2845339 SRX3374354 GSM2845340 GSM2845340: techrep_A+_8h.2; Danio rerio; OTHER SRP124609 SRS2671582 GSM2845340 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845340 GEO Accession GSM2845340 SRX3374355 GSM2845341 GSM2845341: techrep_A+_9h.2; Danio rerio; OTHER SRP124609 SRS2671580 GSM2845341 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845341 GEO Accession GSM2845341 SRX3374356 GSM2845342 GSM2845342: techrep_A+_10h.2; Danio rerio; OTHER SRP124609 SRS2671583 GSM2845342 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845342 GEO Accession GSM2845342 SRX3374357 GSM2845343 GSM2845343: rep_A-_uninjected; Danio rerio; OTHER SRP124609 SRS2671584 GSM2845343 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845343 GEO Accession GSM2845343 SRX3374358 GSM2845344 GSM2845344: rep_A-_1h; Danio rerio; OTHER SRP124609 SRS2671585 GSM2845344 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845344 GEO Accession GSM2845344 SRX3374359 GSM2845345 GSM2845345: rep_A-_2h; Danio rerio; OTHER SRP124609 SRS2671600 GSM2845345 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845345 GEO Accession GSM2845345 SRX3374360 GSM2845346 GSM2845346: rep_A-_3h; Danio rerio; OTHER SRP124609 SRS2671587 GSM2845346 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845346 GEO Accession GSM2845346 SRX3374361 GSM2845347 GSM2845347: rep_A-_4h; Danio rerio; OTHER SRP124609 SRS2671586 GSM2845347 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845347 GEO Accession GSM2845347 SRX3374362 GSM2845348 GSM2845348: rep_A-_6h.1; Danio rerio; OTHER SRP124609 SRS2671588 GSM2845348 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845348 GEO Accession GSM2845348 SRX3374363 GSM2845349 GSM2845349: rep_A-_6h.2; Danio rerio; OTHER SRP124609 SRS2671589 GSM2845349 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845349 GEO Accession GSM2845349 SRX3374364 GSM2845350 GSM2845350: rep_A-_6h.3; Danio rerio; OTHER SRP124609 SRS2671590 GSM2845350 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845350 GEO Accession GSM2845350 SRX3374365 GSM2845351 GSM2845351: rep_A-_8h; Danio rerio; OTHER SRP124609 SRS2671591 GSM2845351 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845351 GEO Accession GSM2845351 SRX3374366 GSM2845352 GSM2845352: rep_A-_10h; Danio rerio; OTHER SRP124609 SRS2671592 GSM2845352 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845352 GEO Accession GSM2845352 SRX3374367 GSM2845353 GSM2845353: oocyte_A-_1h; Danio rerio; OTHER SRP124609 SRS2671602 GSM2845353 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845353 GEO Accession GSM2845353 SRX3374368 GSM2845354 GSM2845354: oocyte_A-_4h; Danio rerio; OTHER SRP124609 SRS2671593 GSM2845354 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845354 GEO Accession GSM2845354 SRX3374369 GSM2845355 GSM2845355: oocyte_A-_7h; Danio rerio; OTHER SRP124609 SRS2671601 GSM2845355 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845355 GEO Accession GSM2845355 SRX3374370 GSM2845356 GSM2845356: oocyte_A+_1h; Danio rerio; OTHER SRP124609 SRS2671595 GSM2845356 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845356 GEO Accession GSM2845356 SRX3374371 GSM2845357 GSM2845357: oocyte_A+_4h; Danio rerio; OTHER SRP124609 SRS2671594 GSM2845357 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845357 GEO Accession GSM2845357 SRX3374372 GSM2845358 GSM2845358: oocyte_A+_7h; Danio rerio; OTHER SRP124609 SRS2671596 GSM2845358 OTHER TRANSCRIPTOMIC other Total RNA was isolated using TRIzol (Invitrogen), after adding 120fg of mRNA with 5 known 3'UTR control sequences into each RNA sample during the initial TRIzol lysis step. In the first step, total-RNA was reverse-transcribed with Maxima RT (Thermo Fisher) and a gene-specific primer that matched the constant 3'UTR of mRNA reporters. RT primer also added a random 8nt UMI and a constant RT adaptor sequence. In the second step, resulting cDNA was amplified by 18 cycles of Phusion PCR with primers that matched the reporter's constant 3'UTR sequence and the RT adaptor. PCR primers also added the appropriate Illumina sample barcodes and sequencing. adaptors Illumina MiSeq gds 302845358 GEO Accession GSM2845358