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    <TITLE>GSM2845403: Rat 61; Rattus norvegicus; RNA-Seq</TITLE>
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    <TITLE>GSM2845404: Rat 64; Rattus norvegicus; RNA-Seq</TITLE>
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      <PRIMARY_ID>SRX3375223</PRIMARY_ID>
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    <TITLE>GSM2845405: Rat 67; Rattus norvegicus; RNA-Seq</TITLE>
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        <PRIMARY_ID>SRP124621</PRIMARY_ID>
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      <PRIMARY_ID>SRX3375224</PRIMARY_ID>
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    <TITLE>GSM2845406: Rat 69; Rattus norvegicus; RNA-Seq</TITLE>
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        <PRIMARY_ID>SRP124621</PRIMARY_ID>
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      <PRIMARY_ID>SRX3375225</PRIMARY_ID>
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    <TITLE>GSM2845407: Rat 71; Rattus norvegicus; RNA-Seq</TITLE>
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        <PRIMARY_ID>SRP124621</PRIMARY_ID>
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          <SINGLE/>
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        <LIBRARY_CONSTRUCTION_PROTOCOL>Illumina TruSeq RNA Sample Prep Kit v2 (Illumina, Inc.) was used with 100–1000 ng of total RNA for the construction of sequencing libraries. In short, total RNA samples were poly(A)-selected and subsequently reverser transcribed into double-stranded cDNA. The following steps were cDNA fragmentation, end-repair, and polyadenylation before ligation of the TruSeq adapters containing the index for multiplexing. We checked the quality and quantity of the libraries with a Qubit (1.0) fluorometer and the Caliper LabChip® GX (Caliper Life Sciences, Inc.). The product had an average fragment size of ∼260bp. We normalized the libraries to 10 nm in 10 mm Tris-Cl, pH 8.5 with 0.1% Tween 20. Total RNA samples were poly(A)-selected and subsequently reverser transcribed into double-stranded cDNA. The following steps were cDNA fragmentation, end-repair, and polyadenylation before ligation of the TruSeq adapters containing the index for multiplexing. We checked the quality and quantity of the libraries with a Qubit (1.0) fluorometer and the Caliper LabChip® GX (Caliper Life Sciences, Inc.). The product had an average fragment size of ∼260bp. We normalized the libraries to 10 nm in 10 mm Tris-Cl, pH 8.5 with 0.1% Tween 20. The TruSeq SR Cluster kit v3-cBot-HS (Illumina, Inc.) was used for cluster generation. Sequencing was performed on the Illumina HiSeq 2000 single end 100 bp using the TruSeq SBS kit v3-HS (Illumina, Inc.).</LIBRARY_CONSTRUCTION_PROTOCOL>
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      <PRIMARY_ID>SRX3375226</PRIMARY_ID>
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    <TITLE>GSM2845408: Rat 88; Rattus norvegicus; RNA-Seq</TITLE>
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        <INSTRUMENT_MODEL>Illumina HiSeq 2000</INSTRUMENT_MODEL>
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