SRP124647 PRJNA417698 Altitude and temperature gradient effect in bacterial communities of three forest ecosystems from Monte Tlaloc, Mexico. Altitude and temperate gradient in forest ecosystems of the Sierra Nevada in central Mexico have seven types of forest ecosystems, including pine (PN), Oyamel (OY) and Encino (EN). These ecosystems present temperature variations due to a natural altitude gradient, which leads to changes in the homeostasis of microbiota in the rhizosphere of these ecosystems. A system of blocks moved in the same place and 300 m below in an altitudinal gradient was used, the change of physicochemical and temperature variables was recorded. The 16S metagenomics ™ system was used for the Ion Torrent ™ platform with HiQ ™ chemistries, the analysis of the sequences was made on the Ion Reporter ™ platform. We detected 2457 OTU, 1693 in EN, 1208 in PN and OY with 1575; the UTOs that presented the highest prevalence in the three ecosystems were the genera Sphingomonas, Bradyrhizobium and Rhodoplanes. The diversity indices indicate that the samples are mega diverse; a correlation of temperature was found only with pH, correlation was found by the greater number of correlations of the OTU Sphingomonas sediminicola and Bradyrhizobium sp. The use of independent culture techniques allowed a broader description of the changes in the rhizospheric microbiota of three temperate forest ecosystems of Mount Tlaloc.