SRS2676236 SAMN07978069 7227 Drosophila melanogaster To investigate the role of Dnmt2 in conferring immunity against bacterial infection in Drosophila melanogaster, we perform Escherichia coli infection in 1-3 day old males and observed variation in survival. Using genome-wide sequencing analysis, we sequenced wild type and mt2 null flies pre and post 6 hours of infection. Transcriptional profiling showed that there are inherent differences between wild and null flies even before infection. After infection both these flies showed induction of the immune response genes but with a higher extent in null flies, suggesting inflammatory responses in these flies after infection at a young age. This study suggests that absence of dnmt2 causes changes in the expression profile of an organism and it also provides novel insights into the determinants underlying the innate immune response in flies. It also reveals how loss of Dnmt2 can cause transcriptional variation in turn mediating overt physiological differences an thus determining infection susceptibility of an organism. strain W1118 isolate not applicable breed not applicable cultivar not applicable ecotype not applicable age 1-3day dev_stage adult sex male tissue whole body biomaterial_provider Deppti Deobagkar Savitribai Phule Pune University birth_date not applicable birth_location not applicable breeding_history not applicable breeding_method not applicable cell_line not applicable cell_subtype not applicable cell_type not applicable collected_by Varada Abhyankar collection_date 2014-09-30 culture_collection not applicable death_date not applicable disease not applicable disease_stage not applicable genotype not applicable geo_loc_name India growth_protocol not applicable health_state not applicable isolation_source not applicable lat_lon not applicable phenotype not applicable sample_type RNA specimen_voucher not applicable store_cond not applicable stud_book_number not applicable treatment not applicable BioSampleModel Model organism or animal