SRX3381259 sediment SRP124740 PRJNA417809 Illumina MiSeq paired-end (2 x 250 bp) sequencing was performed on the V4 hypervariable region of the 16S rRNA gene of extracted DNA. In summary, ribosomal sequences were amplified with a 30-cycle PCR reaction using the HotStarTaq Plus Master Mix Kit (Qiagen, Carlsbad, CA) with a 53ÁC annealing temp using barcoded 515F (5Í-GTG CCA GCM GCC GCG GTA A) primer paired with 806R (5Í-GGA CTA CHV GGG TWT CTA AT) primer. Amplicons were pooled and purified with calibrated Agencourt Ampure XP beads (Agencourt Bioscience Corporation, Beverly, MA). Purified PCR product was used to prepare DNA libraries by following the Illumina TruSeq DNA library preparation protocol and sequenced on an Illumina MiSeq following manufacturerÍs guidelines (Illumina Inc., San Diego, CA). SRS2676811 SAMN08007277 sediment AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3381260 microcosm1 SRP124740 PRJNA417809 Illumina MiSeq paired-end (2 x 250 bp) sequencing was performed on the V4 hypervariable region of the 16S rRNA gene of extracted DNA. In summary, ribosomal sequences were amplified with a 30-cycle PCR reaction using the HotStarTaq Plus Master Mix Kit (Qiagen, Carlsbad, CA) with a 53ÁC annealing temp using barcoded 515F (5Í-GTG CCA GCM GCC GCG GTA A) primer paired with 806R (5Í-GGA CTA CHV GGG TWT CTA AT) primer. Amplicons were pooled and purified with calibrated Agencourt Ampure XP beads (Agencourt Bioscience Corporation, Beverly, MA). Purified PCR product was used to prepare DNA libraries by following the Illumina TruSeq DNA library preparation protocol and sequenced on an Illumina MiSeq following manufacturerÍs guidelines (Illumina Inc., San Diego, CA). SRS2676812 SAMN08007134 microcosm1 AMPLICON METAGENOMIC PCR Illumina MiSeq SRX3381261 microcosm2 SRP124740 PRJNA417809 Illumina MiSeq paired-end (2 x 250 bp) sequencing was performed on the V4 hypervariable region of the 16S rRNA gene of extracted DNA. In summary, ribosomal sequences were amplified with a 30-cycle PCR reaction using the HotStarTaq Plus Master Mix Kit (Qiagen, Carlsbad, CA) with a 53ÁC annealing temp using barcoded 515F (5Í-GTG CCA GCM GCC GCG GTA A) primer paired with 806R (5Í-GGA CTA CHV GGG TWT CTA AT) primer. Amplicons were pooled and purified with calibrated Agencourt Ampure XP beads (Agencourt Bioscience Corporation, Beverly, MA). Purified PCR product was used to prepare DNA libraries by following the Illumina TruSeq DNA library preparation protocol and sequenced on an Illumina MiSeq following manufacturerÍs guidelines (Illumina Inc., San Diego, CA). SRS2676813 SAMN08007276 microcosm2 AMPLICON METAGENOMIC PCR Illumina MiSeq