SRX3383591 GSM2850131 SRP124778 SRS2679117 GSM2850131 RNA-Seq TRANSCRIPTOMIC cDNA For RIP-Seq：Cells were washed twice with cold PBS and the cell pellet was re-suspended with 2 volumes of lysis buffer (150 mM KCl, 10 mM HEPES pH 7.6, 2 mM EDTA, 0.5% NP-40, 0.5 mM DTT, 1:100 protease inhibitor cocktail, 400 U/mL RNase inhibitor).For RNA-Seq: Total RNA was extracted by Trizol. For RIP-Seq：Lysate was incubated on ice for 5 min centrifuged for 15 min to clear the lysate. 1/10 volume of cell lysate was saved as input and total RNA was extracted by trizol. The rest of cell lysate was incubated with 5ug anti-YTHDF1(Proteintech) at 4°C overnight with gentle rotation followed by incubation with 40ul protein G beads for 1 hour at 4°C. The beads were then washed five times with 1 mL ice-cold washing buffer (200 mM NaCl, 50 mM HEPES pH 7.6, 2 mM EDTA, 0.05% NP-40, 0.5 mM DTT, 200 U/mL RNase inhibitor). The IP complex was re-suspended in 400μl 1xProteinase K and digested with 2 mg Proteinase K at 55°C for 1 hour. RNA was then extracted by RNA isolation kit (Zymo). Input and IP RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). For RNA-Seq: Extracted RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). Illumina HiSeq 2000 gds 302850131 GSM2850131 GEO Accession GSM2850131 SRX3383592 GSM2850132 SRP124778 SRS2679118 GSM2850132 RNA-Seq TRANSCRIPTOMIC cDNA For RIP-Seq：Cells were washed twice with cold PBS and the cell pellet was re-suspended with 2 volumes of lysis buffer (150 mM KCl, 10 mM HEPES pH 7.6, 2 mM EDTA, 0.5% NP-40, 0.5 mM DTT, 1:100 protease inhibitor cocktail, 400 U/mL RNase inhibitor).For RNA-Seq: Total RNA was extracted by Trizol. For RIP-Seq：Lysate was incubated on ice for 5 min centrifuged for 15 min to clear the lysate. 1/10 volume of cell lysate was saved as input and total RNA was extracted by trizol. The rest of cell lysate was incubated with 5ug anti-YTHDF1(Proteintech) at 4°C overnight with gentle rotation followed by incubation with 40ul protein G beads for 1 hour at 4°C. The beads were then washed five times with 1 mL ice-cold washing buffer (200 mM NaCl, 50 mM HEPES pH 7.6, 2 mM EDTA, 0.05% NP-40, 0.5 mM DTT, 200 U/mL RNase inhibitor). The IP complex was re-suspended in 400μl 1xProteinase K and digested with 2 mg Proteinase K at 55°C for 1 hour. RNA was then extracted by RNA isolation kit (Zymo). Input and IP RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). For RNA-Seq: Extracted RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). Illumina HiSeq 2000 gds 302850132 GSM2850132 GEO Accession GSM2850132 SRX3383593 GSM2850133 SRP124778 SRS2679120 GSM2850133 RNA-Seq TRANSCRIPTOMIC cDNA For RIP-Seq：Cells were washed twice with cold PBS and the cell pellet was re-suspended with 2 volumes of lysis buffer (150 mM KCl, 10 mM HEPES pH 7.6, 2 mM EDTA, 0.5% NP-40, 0.5 mM DTT, 1:100 protease inhibitor cocktail, 400 U/mL RNase inhibitor).For RNA-Seq: Total RNA was extracted by Trizol. For RIP-Seq：Lysate was incubated on ice for 5 min centrifuged for 15 min to clear the lysate. 1/10 volume of cell lysate was saved as input and total RNA was extracted by trizol. The rest of cell lysate was incubated with 5ug anti-YTHDF1(Proteintech) at 4°C overnight with gentle rotation followed by incubation with 40ul protein G beads for 1 hour at 4°C. The beads were then washed five times with 1 mL ice-cold washing buffer (200 mM NaCl, 50 mM HEPES pH 7.6, 2 mM EDTA, 0.05% NP-40, 0.5 mM DTT, 200 U/mL RNase inhibitor). The IP complex was re-suspended in 400μl 1xProteinase K and digested with 2 mg Proteinase K at 55°C for 1 hour. RNA was then extracted by RNA isolation kit (Zymo). Input and IP RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). For RNA-Seq: Extracted RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). Illumina HiSeq 2000 gds 302850133 GSM2850133 GEO Accession GSM2850133 SRX3383594 GSM2850134 SRP124778 SRS2679119 GSM2850134 RNA-Seq TRANSCRIPTOMIC cDNA For RIP-Seq：Cells were washed twice with cold PBS and the cell pellet was re-suspended with 2 volumes of lysis buffer (150 mM KCl, 10 mM HEPES pH 7.6, 2 mM EDTA, 0.5% NP-40, 0.5 mM DTT, 1:100 protease inhibitor cocktail, 400 U/mL RNase inhibitor).For RNA-Seq: Total RNA was extracted by Trizol. For RIP-Seq：Lysate was incubated on ice for 5 min centrifuged for 15 min to clear the lysate. 1/10 volume of cell lysate was saved as input and total RNA was extracted by trizol. The rest of cell lysate was incubated with 5ug anti-YTHDF1(Proteintech) at 4°C overnight with gentle rotation followed by incubation with 40ul protein G beads for 1 hour at 4°C. The beads were then washed five times with 1 mL ice-cold washing buffer (200 mM NaCl, 50 mM HEPES pH 7.6, 2 mM EDTA, 0.05% NP-40, 0.5 mM DTT, 200 U/mL RNase inhibitor). The IP complex was re-suspended in 400μl 1xProteinase K and digested with 2 mg Proteinase K at 55°C for 1 hour. RNA was then extracted by RNA isolation kit (Zymo). Input and IP RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). For RNA-Seq: Extracted RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). Illumina HiSeq 2000 gds 302850134 GSM2850134 GEO Accession GSM2850134 SRX3383595 GSM2850135 SRP124778 SRS2679121 GSM2850135 RNA-Seq TRANSCRIPTOMIC cDNA For RIP-Seq：Cells were washed twice with cold PBS and the cell pellet was re-suspended with 2 volumes of lysis buffer (150 mM KCl, 10 mM HEPES pH 7.6, 2 mM EDTA, 0.5% NP-40, 0.5 mM DTT, 1:100 protease inhibitor cocktail, 400 U/mL RNase inhibitor).For RNA-Seq: Total RNA was extracted by Trizol. For RIP-Seq：Lysate was incubated on ice for 5 min centrifuged for 15 min to clear the lysate. 1/10 volume of cell lysate was saved as input and total RNA was extracted by trizol. The rest of cell lysate was incubated with 5ug anti-YTHDF1(Proteintech) at 4°C overnight with gentle rotation followed by incubation with 40ul protein G beads for 1 hour at 4°C. The beads were then washed five times with 1 mL ice-cold washing buffer (200 mM NaCl, 50 mM HEPES pH 7.6, 2 mM EDTA, 0.05% NP-40, 0.5 mM DTT, 200 U/mL RNase inhibitor). The IP complex was re-suspended in 400μl 1xProteinase K and digested with 2 mg Proteinase K at 55°C for 1 hour. RNA was then extracted by RNA isolation kit (Zymo). Input and IP RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). For RNA-Seq: Extracted RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). Illumina HiSeq 2000 gds 302850135 GSM2850135 GEO Accession GSM2850135 SRX3383596 GSM2850136 SRP124778 SRS2679122 GSM2850136 RNA-Seq TRANSCRIPTOMIC cDNA For RIP-Seq：Cells were washed twice with cold PBS and the cell pellet was re-suspended with 2 volumes of lysis buffer (150 mM KCl, 10 mM HEPES pH 7.6, 2 mM EDTA, 0.5% NP-40, 0.5 mM DTT, 1:100 protease inhibitor cocktail, 400 U/mL RNase inhibitor).For RNA-Seq: Total RNA was extracted by Trizol. For RIP-Seq：Lysate was incubated on ice for 5 min centrifuged for 15 min to clear the lysate. 1/10 volume of cell lysate was saved as input and total RNA was extracted by trizol. The rest of cell lysate was incubated with 5ug anti-YTHDF1(Proteintech) at 4°C overnight with gentle rotation followed by incubation with 40ul protein G beads for 1 hour at 4°C. The beads were then washed five times with 1 mL ice-cold washing buffer (200 mM NaCl, 50 mM HEPES pH 7.6, 2 mM EDTA, 0.05% NP-40, 0.5 mM DTT, 200 U/mL RNase inhibitor). The IP complex was re-suspended in 400μl 1xProteinase K and digested with 2 mg Proteinase K at 55°C for 1 hour. RNA was then extracted by RNA isolation kit (Zymo). Input and IP RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). For RNA-Seq: Extracted RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). Illumina HiSeq 2000 gds 302850136 GSM2850136 GEO Accession GSM2850136 SRX3383597 GSM2850137 SRP124778 SRS2679128 GSM2850137 RNA-Seq TRANSCRIPTOMIC cDNA For RIP-Seq：Cells were washed twice with cold PBS and the cell pellet was re-suspended with 2 volumes of lysis buffer (150 mM KCl, 10 mM HEPES pH 7.6, 2 mM EDTA, 0.5% NP-40, 0.5 mM DTT, 1:100 protease inhibitor cocktail, 400 U/mL RNase inhibitor).For RNA-Seq: Total RNA was extracted by Trizol. For RIP-Seq：Lysate was incubated on ice for 5 min centrifuged for 15 min to clear the lysate. 1/10 volume of cell lysate was saved as input and total RNA was extracted by trizol. The rest of cell lysate was incubated with 5ug anti-YTHDF1(Proteintech) at 4°C overnight with gentle rotation followed by incubation with 40ul protein G beads for 1 hour at 4°C. The beads were then washed five times with 1 mL ice-cold washing buffer (200 mM NaCl, 50 mM HEPES pH 7.6, 2 mM EDTA, 0.05% NP-40, 0.5 mM DTT, 200 U/mL RNase inhibitor). The IP complex was re-suspended in 400μl 1xProteinase K and digested with 2 mg Proteinase K at 55°C for 1 hour. RNA was then extracted by RNA isolation kit (Zymo). Input and IP RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). For RNA-Seq: Extracted RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). Illumina HiSeq 2000 gds 302850137 GSM2850137 GEO Accession GSM2850137 SRX3383598 GSM2850138 SRP124778 SRS2679123 GSM2850138 RNA-Seq TRANSCRIPTOMIC cDNA For RIP-Seq：Cells were washed twice with cold PBS and the cell pellet was re-suspended with 2 volumes of lysis buffer (150 mM KCl, 10 mM HEPES pH 7.6, 2 mM EDTA, 0.5% NP-40, 0.5 mM DTT, 1:100 protease inhibitor cocktail, 400 U/mL RNase inhibitor).For RNA-Seq: Total RNA was extracted by Trizol. For RIP-Seq：Lysate was incubated on ice for 5 min centrifuged for 15 min to clear the lysate. 1/10 volume of cell lysate was saved as input and total RNA was extracted by trizol. The rest of cell lysate was incubated with 5ug anti-YTHDF1(Proteintech) at 4°C overnight with gentle rotation followed by incubation with 40ul protein G beads for 1 hour at 4°C. The beads were then washed five times with 1 mL ice-cold washing buffer (200 mM NaCl, 50 mM HEPES pH 7.6, 2 mM EDTA, 0.05% NP-40, 0.5 mM DTT, 200 U/mL RNase inhibitor). The IP complex was re-suspended in 400μl 1xProteinase K and digested with 2 mg Proteinase K at 55°C for 1 hour. RNA was then extracted by RNA isolation kit (Zymo). Input and IP RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). For RNA-Seq: Extracted RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). Illumina HiSeq 2000 gds 302850138 GSM2850138 GEO Accession GSM2850138 SRX3383599 GSM2850139 SRP124778 SRS2679124 GSM2850139 RIP-Seq TRANSCRIPTOMIC other For RIP-Seq：Cells were washed twice with cold PBS and the cell pellet was re-suspended with 2 volumes of lysis buffer (150 mM KCl, 10 mM HEPES pH 7.6, 2 mM EDTA, 0.5% NP-40, 0.5 mM DTT, 1:100 protease inhibitor cocktail, 400 U/mL RNase inhibitor).For RNA-Seq: Total RNA was extracted by Trizol. For RIP-Seq：Lysate was incubated on ice for 5 min centrifuged for 15 min to clear the lysate. 1/10 volume of cell lysate was saved as input and total RNA was extracted by trizol. The rest of cell lysate was incubated with 5ug anti-YTHDF1(Proteintech) at 4°C overnight with gentle rotation followed by incubation with 40ul protein G beads for 1 hour at 4°C. The beads were then washed five times with 1 mL ice-cold washing buffer (200 mM NaCl, 50 mM HEPES pH 7.6, 2 mM EDTA, 0.05% NP-40, 0.5 mM DTT, 200 U/mL RNase inhibitor). The IP complex was re-suspended in 400μl 1xProteinase K and digested with 2 mg Proteinase K at 55°C for 1 hour. RNA was then extracted by RNA isolation kit (Zymo). Input and IP RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). For RNA-Seq: Extracted RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). Illumina HiSeq 2000 gds 302850139 GSM2850139 GEO Accession GSM2850139 SRX3383600 GSM2850140 SRP124778 SRS2679125 GSM2850140 RIP-Seq TRANSCRIPTOMIC other For RIP-Seq：Cells were washed twice with cold PBS and the cell pellet was re-suspended with 2 volumes of lysis buffer (150 mM KCl, 10 mM HEPES pH 7.6, 2 mM EDTA, 0.5% NP-40, 0.5 mM DTT, 1:100 protease inhibitor cocktail, 400 U/mL RNase inhibitor).For RNA-Seq: Total RNA was extracted by Trizol. For RIP-Seq：Lysate was incubated on ice for 5 min centrifuged for 15 min to clear the lysate. 1/10 volume of cell lysate was saved as input and total RNA was extracted by trizol. The rest of cell lysate was incubated with 5ug anti-YTHDF1(Proteintech) at 4°C overnight with gentle rotation followed by incubation with 40ul protein G beads for 1 hour at 4°C. The beads were then washed five times with 1 mL ice-cold washing buffer (200 mM NaCl, 50 mM HEPES pH 7.6, 2 mM EDTA, 0.05% NP-40, 0.5 mM DTT, 200 U/mL RNase inhibitor). The IP complex was re-suspended in 400μl 1xProteinase K and digested with 2 mg Proteinase K at 55°C for 1 hour. RNA was then extracted by RNA isolation kit (Zymo). Input and IP RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). For RNA-Seq: Extracted RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). Illumina HiSeq 2000 gds 302850140 GSM2850140 GEO Accession GSM2850140 SRX3383601 GSM2850141 SRP124778 SRS2679126 GSM2850141 RIP-Seq TRANSCRIPTOMIC other For RIP-Seq：Cells were washed twice with cold PBS and the cell pellet was re-suspended with 2 volumes of lysis buffer (150 mM KCl, 10 mM HEPES pH 7.6, 2 mM EDTA, 0.5% NP-40, 0.5 mM DTT, 1:100 protease inhibitor cocktail, 400 U/mL RNase inhibitor).For RNA-Seq: Total RNA was extracted by Trizol. For RIP-Seq：Lysate was incubated on ice for 5 min centrifuged for 15 min to clear the lysate. 1/10 volume of cell lysate was saved as input and total RNA was extracted by trizol. The rest of cell lysate was incubated with 5ug anti-YTHDF1(Proteintech) at 4°C overnight with gentle rotation followed by incubation with 40ul protein G beads for 1 hour at 4°C. The beads were then washed five times with 1 mL ice-cold washing buffer (200 mM NaCl, 50 mM HEPES pH 7.6, 2 mM EDTA, 0.05% NP-40, 0.5 mM DTT, 200 U/mL RNase inhibitor). The IP complex was re-suspended in 400μl 1xProteinase K and digested with 2 mg Proteinase K at 55°C for 1 hour. RNA was then extracted by RNA isolation kit (Zymo). Input and IP RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). For RNA-Seq: Extracted RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). Illumina HiSeq 2000 gds 302850141 GSM2850141 GEO Accession GSM2850141 SRX3383602 GSM2850142 SRP124778 SRS2679127 GSM2850142 RIP-Seq TRANSCRIPTOMIC other For RIP-Seq：Cells were washed twice with cold PBS and the cell pellet was re-suspended with 2 volumes of lysis buffer (150 mM KCl, 10 mM HEPES pH 7.6, 2 mM EDTA, 0.5% NP-40, 0.5 mM DTT, 1:100 protease inhibitor cocktail, 400 U/mL RNase inhibitor).For RNA-Seq: Total RNA was extracted by Trizol. For RIP-Seq：Lysate was incubated on ice for 5 min centrifuged for 15 min to clear the lysate. 1/10 volume of cell lysate was saved as input and total RNA was extracted by trizol. The rest of cell lysate was incubated with 5ug anti-YTHDF1(Proteintech) at 4°C overnight with gentle rotation followed by incubation with 40ul protein G beads for 1 hour at 4°C. The beads were then washed five times with 1 mL ice-cold washing buffer (200 mM NaCl, 50 mM HEPES pH 7.6, 2 mM EDTA, 0.05% NP-40, 0.5 mM DTT, 200 U/mL RNase inhibitor). The IP complex was re-suspended in 400μl 1xProteinase K and digested with 2 mg Proteinase K at 55°C for 1 hour. RNA was then extracted by RNA isolation kit (Zymo). Input and IP RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). For RNA-Seq: Extracted RNA of each sample were used to generate the library using TruSeq stranded mRNA sample preparation kit (Illumina). Illumina HiSeq 2000 gds 302850142 GSM2850142 GEO Accession GSM2850142