SRX3398845 GSM2858931 SRP125102 SRS2693025 GSM2858931 ChIP-Seq GENOMIC ChIP Sciatic nerves were subjected to MNase-ChIP with anti-H3K27me3 antibody from Active Motif (AM39155) as described previously with minor modification (Ma et al., 2015). After incubation with Dynabeads Protein G (Invitrogen, 10004D), ChIP samples were washed with washing buffer 1 (WB1, 50 mM Tris-HCl, pH7.5; 10 mM EDTA; 125 mM NaCl) once, WB2 (50 mM Tris-HCl, pH7.5; 10 mM EDTA; 250 mM NaCl) once, and WB3 (50 mM Tris-HCl, pH7.5; 10 mM EDTA; 500 mM NaCl) twice. The samples then eluted at 65°C with elution buffer (50 mM NaCl; 50 mM Tris-HCl, pH 7.5; 5mM EDTA; 1% SDS) for 15 min. Libraries were prepared from ChIP DNA, and respective inputs, using the Illumina TruSeq ChIP Sample Preparation Kit. Illumina HiSeq 2500 gds 302858931 GSM2858931 GEO Accession GSM2858931 SRX3398846 GSM2858932 SRP125102 SRS2693026 GSM2858932 ChIP-Seq GENOMIC ChIP Sciatic nerves were subjected to MNase-ChIP with anti-H3K27me3 antibody from Active Motif (AM39155) as described previously with minor modification (Ma et al., 2015). After incubation with Dynabeads Protein G (Invitrogen, 10004D), ChIP samples were washed with washing buffer 1 (WB1, 50 mM Tris-HCl, pH7.5; 10 mM EDTA; 125 mM NaCl) once, WB2 (50 mM Tris-HCl, pH7.5; 10 mM EDTA; 250 mM NaCl) once, and WB3 (50 mM Tris-HCl, pH7.5; 10 mM EDTA; 500 mM NaCl) twice. The samples then eluted at 65°C with elution buffer (50 mM NaCl; 50 mM Tris-HCl, pH 7.5; 5mM EDTA; 1% SDS) for 15 min. Libraries were prepared from ChIP DNA, and respective inputs, using the Illumina TruSeq ChIP Sample Preparation Kit. Illumina HiSeq 2500 gds 302858932 GSM2858932 GEO Accession GSM2858932 SRX3398847 GSM2858933 SRP125102 SRS2693027 GSM2858933 ChIP-Seq GENOMIC ChIP Sciatic nerves were subjected to MNase-ChIP with anti-H3K27me3 antibody from Active Motif (AM39155) as described previously with minor modification (Ma et al., 2015). After incubation with Dynabeads Protein G (Invitrogen, 10004D), ChIP samples were washed with washing buffer 1 (WB1, 50 mM Tris-HCl, pH7.5; 10 mM EDTA; 125 mM NaCl) once, WB2 (50 mM Tris-HCl, pH7.5; 10 mM EDTA; 250 mM NaCl) once, and WB3 (50 mM Tris-HCl, pH7.5; 10 mM EDTA; 500 mM NaCl) twice. The samples then eluted at 65°C with elution buffer (50 mM NaCl; 50 mM Tris-HCl, pH 7.5; 5mM EDTA; 1% SDS) for 15 min. Libraries were prepared from ChIP DNA, and respective inputs, using the Illumina TruSeq ChIP Sample Preparation Kit. Illumina HiSeq 2500 gds 302858933 GSM2858933 GEO Accession GSM2858933 SRX3398848 GSM2858934 SRP125102 SRS2693028 GSM2858934 ChIP-Seq GENOMIC ChIP Sciatic nerves were subjected to MNase-ChIP with anti-H3K27me3 antibody from Active Motif (AM39155) as described previously with minor modification (Ma et al., 2015). After incubation with Dynabeads Protein G (Invitrogen, 10004D), ChIP samples were washed with washing buffer 1 (WB1, 50 mM Tris-HCl, pH7.5; 10 mM EDTA; 125 mM NaCl) once, WB2 (50 mM Tris-HCl, pH7.5; 10 mM EDTA; 250 mM NaCl) once, and WB3 (50 mM Tris-HCl, pH7.5; 10 mM EDTA; 500 mM NaCl) twice. The samples then eluted at 65°C with elution buffer (50 mM NaCl; 50 mM Tris-HCl, pH 7.5; 5mM EDTA; 1% SDS) for 15 min. Libraries were prepared from ChIP DNA, and respective inputs, using the Illumina TruSeq ChIP Sample Preparation Kit. Illumina HiSeq 2500 gds 302858934 GSM2858934 GEO Accession GSM2858934