SRX3407632 Ago2_NCD_1 SRP113546 PRJNA395686 RNA was extracted by miRNeasy micro Kit (Qiagen) with total RNA extraction protocol. NEBNext small RNA library preparation kit (New England BioLabs, Ipswich, MA) was used for library preparation. Libraries at the final concentration of 10 pM were clustered onto a single read (SR) flow cell using Illumina TruSeq SR Cluster kit v3, and sequenced for 50 cycles using TruSeq SBS kit on Illumina HiSeq system. SRS2700673 SAMN07415658 Ago2_NCD_1 miRNA-Seq TRANSCRIPTOMIC size fractionation Illumina HiSeq 2500 SRX3407633 Ago2_koNCD_1 SRP113546 PRJNA395686 RNA was extracted by miRNeasy micro Kit (Qiagen) with total RNA extraction protocol. NEBNext small RNA library preparation kit (New England BioLabs, Ipswich, MA) was used for library preparation. Libraries at the final concentration of 10 pM were clustered onto a single read (SR) flow cell using Illumina TruSeq SR Cluster kit v3, and sequenced for 50 cycles using TruSeq SBS kit on Illumina HiSeq system. SRS2700672 SAMN07415659 Ago2_koNCD_1 miRNA-Seq TRANSCRIPTOMIC size fractionation Illumina HiSeq 2500