SRX3408662 GSM2861589 SRP125289 SRS2705890 GSM2861589 RNA-Seq TRANSCRIPTOMIC cDNA Cells were harvested at 24 hours after plating. TRIzol reagent was added directly into the plate after cold 1 × PBS wash once. The total RNA was extracted according to manufacturer's instructions. mRNA was further isolated by Dynabeads mRNA purification kit (Life Technologies) according to manufacturer's instructions. RNAs were treated with DNase I for 20 minutes to remove the residue DNA, and reverse-transcribed with SuperScript III Reverse Transcriptase (ThermoFisher). Sequence specific RT primer that targeting the downstream of insertion site of RSLP library was used for the reverse-transcription. The reverse-transcription was performed at 50℃ for 40 minutes and 55℃ for another 20 minutes. 10 U exonuclease I was added and incubate at 37℃ for 20 minute to remove the free RT primers. The reaction was stopped by boil the sample at 95℃ for 10 minutes to inactivate all the enzymes. 0.5 ul 10 mg/ml RNase A was added and incubate at 37℃ for 20 minutes to degrade the RNA. Ethanol precipitation and downstream wash steps were performed as described before. The purified cDNAs were then used as templates for PCR and library construction. Library was prepared by NEBNext Ultra II DNA library preparation kit according to the standard protocols provided by the manufacturer. RNA-Seq of RSLP targeted regions HiSeq X Ten gds 302861589 GSM2861589 GEO Accession GSM2861589 SRX3408663 GSM2861590 SRP125289 SRS2705891 GSM2861590 RNA-Seq TRANSCRIPTOMIC cDNA Cells were harvested at 24 hours after plating. TRIzol reagent was added directly into the plate after cold 2 × PBS wash once. The total RNA was extracted according to manufacturer's instructions. mRNA was further isolated by Dynabeads mRNA purification k RNA-Seq of RSLP targeted regions HiSeq X Ten gds 302861590 GSM2861590 GEO Accession GSM2861590 SRX3408664 GSM2861591 SRP125289 SRS2705901 GSM2861591 RNA-Seq TRANSCRIPTOMIC cDNA Cells were harvested at 24 hours after plating. TRIzol reagent was added directly into the plate after cold 3 × PBS wash once. The total RNA was extracted according to manufacturer's instructions. mRNA was further isolated by Dynabeads mRNA purification k RNA-Seq of RSLP targeted regions HiSeq X Ten gds 302861591 GSM2861591 GEO Accession GSM2861591 SRX3408665 GSM2861592 SRP125289 SRS2705892 GSM2861592 RNA-Seq TRANSCRIPTOMIC cDNA Cells were harvested at 24 hours after plating. TRIzol reagent was added directly into the plate after cold 4 × PBS wash once. The total RNA was extracted according to manufacturer's instructions. mRNA was further isolated by Dynabeads mRNA purification k RNA-Seq of RSLP targeted regions HiSeq X Ten gds 302861592 GSM2861592 GEO Accession GSM2861592 SRX3408666 GSM2861593 SRP125289 SRS2705894 GSM2861593 RNA-Seq TRANSCRIPTOMIC cDNA Cells were harvested at 24 hours after plating. TRIzol reagent was added directly into the plate after cold 5 × PBS wash once. The total RNA was extracted according to manufacturer's instructions. mRNA was further isolated by Dynabeads mRNA purification k RNA-Seq of RSLP targeted regions HiSeq X Ten gds 302861593 GSM2861593 GEO Accession GSM2861593 SRX3408667 GSM2861594 SRP125289 SRS2705893 GSM2861594 RNA-Seq TRANSCRIPTOMIC cDNA Cells were harvested at 24 hours after plating. TRIzol reagent was added directly into the plate after cold 6 × PBS wash once. The total RNA was extracted according to manufacturer's instructions. mRNA was further isolated by Dynabeads mRNA purification k RNA-Seq of RSLP targeted regions HiSeq X Ten gds 302861594 GSM2861594 GEO Accession GSM2861594 SRX3408668 GSM2861595 SRP125289 SRS2705895 GSM2861595 RNA-Seq TRANSCRIPTOMIC cDNA Cells were harvested at 24 hours after plating. TRIzol reagent was added directly into the plate after cold 7 × PBS wash once. The total RNA was extracted according to manufacturer's instructions. mRNA was further isolated by Dynabeads mRNA purification k RNA-Seq of RSLP targeted regions HiSeq X Ten gds 302861595 GSM2861595 GEO Accession GSM2861595 SRX3408669 GSM2861596 SRP125289 SRS2705898 GSM2861596 RNA-Seq TRANSCRIPTOMIC cDNA Cells were harvested at 24 hours after plating. TRIzol reagent was added directly into the plate after cold 8 × PBS wash once. The total RNA was extracted according to manufacturer's instructions. mRNA was further isolated by Dynabeads mRNA purification k RNA-Seq of RSLP targeted regions HiSeq X Ten gds 302861596 GSM2861596 GEO Accession GSM2861596 SRX3408670 GSM2861597 SRP125289 SRS2705900 GSM2861597 RNA-Seq TRANSCRIPTOMIC cDNA Cells were harvested at 24 hours after plating. TRIzol reagent was added directly into the plate after cold 9 × PBS wash once. The total RNA was extracted according to manufacturer's instructions. mRNA was further isolated by Dynabeads mRNA purification k RNA-Seq of RSLP targeted regions HiSeq X Ten gds 302861597 GSM2861597 GEO Accession GSM2861597 SRX3408671 GSM2861598 SRP125289 SRS2705896 GSM2861598 RNA-Seq TRANSCRIPTOMIC cDNA Cells were harvested at 24 hours after plating. TRIzol reagent was added directly into the plate after cold10 × PBS wash once. The total RNA was extracted according to manufacturer's instructions. mRNA was further isolated by Dynabeads mRNA purification k RNA-Seq of RSLP targeted regions HiSeq X Ten gds 302861598 GSM2861598 GEO Accession GSM2861598 SRX3408672 GSM2861599 SRP125289 SRS2705905 GSM2861599 RNA-Seq TRANSCRIPTOMIC cDNA Cells were harvested at 24 hours after plating. TRIzol reagent was added directly into the plate after cold11 × PBS wash once. The total RNA was extracted according to manufacturer's instructions. mRNA was further isolated by Dynabeads mRNA purification k RNA-Seq of RSLP targeted regions HiSeq X Ten gds 302861599 GSM2861599 GEO Accession GSM2861599 SRX3408673 GSM2861600 SRP125289 SRS2705897 GSM2861600 RNA-Seq TRANSCRIPTOMIC cDNA Cells were harvested at 24 hours after plating. TRIzol reagent was added directly into the plate after cold12 × PBS wash once. The total RNA was extracted according to manufacturer's instructions. mRNA was further isolated by Dynabeads mRNA purification k RNA-Seq of RSLP targeted regions HiSeq X Ten gds 302861600 GSM2861600 GEO Accession GSM2861600