SRX3410147 290117 SRP125323 bp0 Plasmodiophorid-specific primer pair 1301f (5'-GATTGAAGCTCTTTCTTGATCACTTC-3') and 1801gram (5'-ACGGAAACCTTGTTACGACTTC-3), which amplify the V6-V9 region of the 18S rRNA gene (18S rDNA). PCR reactions were carried out using 1 µl of DNA (at a final reaction concentration of 0.2 µM) extracted from soil and rhizosphere samples in 24 µl MyTaq HS mastermix (Bioline, London UK). 10-bp MIDs (www.454.com) and A and B 454 adaptors were then ligated onto the amplicons, samples were equimolar pooled following quantitation using a Shimadzu MultiNA (Milton Keynes, UK). Sequencing was performed on a Roche 454 GS Junior pyrosequencer (454 Life Sciences/Roche Applied Biosystems, Nurley, NJ, USA) at Micropathology Ltd (Coventry, UK) entirely according to the manufacturers protocol with no deviations (libL emPCR kit) (Roche 454 Sequencing system software manual, v 2.5p1). SRS2703224 SAMN08043899 290117 AMPLICON OTHER PCR 454 GS Junior SRX3410148 240117 SRP125323 bp0 Plasmodiophorid-specific primer pair 1301f (5'-GATTGAAGCTCTTTCTTGATCACTTC-3') and 1801gram (5'-ACGGAAACCTTGTTACGACTTC-3), which amplify the V6-V9 region of the 18S rRNA gene (18S rDNA). PCR reactions were carried out using 1 µl of DNA (at a final reaction concentration of 0.2 µM) extracted from soil and rhizosphere samples in 24 µl MyTaq HS mastermix (Bioline, London UK). 10-bp MIDs (www.454.com) and A and B 454 adaptors were then ligated onto the amplicons, samples were equimolar pooled following quantitation using a Shimadzu MultiNA (Milton Keynes, UK). Sequencing was performed on a Roche 454 GS Junior pyrosequencer (454 Life Sciences/Roche Applied Biosystems, Nurley, NJ, USA) at Micropathology Ltd (Coventry, UK) entirely according to the manufacturers protocol with no deviations (libL emPCR kit) (Roche 454 Sequencing system software manual, v 2.5p1). SRS2703225 SAMN08043898 240117 AMPLICON OTHER PCR 454 GS Junior