SRX3426729 GSM2867523 SRP125767 SRS2725677 GSM2867523 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867523 GSM2867523 GEO Accession GSM2867523 SRX3426730 GSM2867524 SRP125767 SRS2725671 GSM2867524 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867524 GSM2867524 GEO Accession GSM2867524 SRX3426731 GSM2867525 SRP125767 SRS2725674 GSM2867525 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867525 GSM2867525 GEO Accession GSM2867525 SRX3426732 GSM2867526 SRP125767 SRS2725672 GSM2867526 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867526 GSM2867526 GEO Accession GSM2867526 SRX3426733 GSM2867527 SRP125767 SRS2725675 GSM2867527 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867527 GSM2867527 GEO Accession GSM2867527 SRX3426734 GSM2867528 SRP125767 SRS2718247 GSM2867528 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867528 GSM2867528 GEO Accession GSM2867528 SRX3426735 GSM2867529 SRP125767 SRS2718248 GSM2867529 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867529 GSM2867529 GEO Accession GSM2867529 SRX3426736 GSM2867530 SRP125767 SRS2718249 GSM2867530 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867530 GSM2867530 GEO Accession GSM2867530 SRX3426737 GSM2867531 SRP125767 SRS2718250 GSM2867531 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867531 GSM2867531 GEO Accession GSM2867531 SRX3426738 GSM2867532 SRP125767 SRS2718251 GSM2867532 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867532 GSM2867532 GEO Accession GSM2867532 SRX3426739 GSM2867533 SRP125767 SRS2718252 GSM2867533 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867533 GSM2867533 GEO Accession GSM2867533 SRX3426740 GSM2867534 SRP125767 SRS2725673 GSM2867534 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867534 GSM2867534 GEO Accession GSM2867534 SRX3426741 GSM2867535 SRP125767 SRS2725676 GSM2867535 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867535 GSM2867535 GEO Accession GSM2867535 SRX3426742 GSM2867536 SRP125767 SRS2718253 GSM2867536 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867536 GSM2867536 GEO Accession GSM2867536 SRX3426743 GSM2867537 SRP125767 SRS2718254 GSM2867537 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867537 GSM2867537 GEO Accession GSM2867537 SRX3426744 GSM2867538 SRP125767 SRS2718255 GSM2867538 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867538 GSM2867538 GEO Accession GSM2867538 SRX3426745 GSM2867539 SRP125767 SRS2718256 GSM2867539 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867539 GSM2867539 GEO Accession GSM2867539 SRX3426746 GSM2867540 SRP125767 SRS2718257 GSM2867540 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867540 GSM2867540 GEO Accession GSM2867540 SRX3426747 GSM2867541 SRP125767 SRS2718258 GSM2867541 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867541 GSM2867541 GEO Accession GSM2867541 SRX3426748 GSM2867542 SRP125767 SRS2718259 GSM2867542 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867542 GSM2867542 GEO Accession GSM2867542 SRX3426749 GSM2867543 SRP125767 SRS2718260 GSM2867543 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867543 GSM2867543 GEO Accession GSM2867543 SRX3426750 GSM2867544 SRP125767 SRS2718261 GSM2867544 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867544 GSM2867544 GEO Accession GSM2867544 SRX3426751 GSM2867545 SRP125767 SRS2718262 GSM2867545 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867545 GSM2867545 GEO Accession GSM2867545 SRX3426752 GSM2867546 SRP125767 SRS2718263 GSM2867546 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867546 GSM2867546 GEO Accession GSM2867546 SRX3426753 GSM2867547 SRP125767 SRS2718264 GSM2867547 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867547 GSM2867547 GEO Accession GSM2867547 SRX3426754 GSM2867548 SRP125767 SRS2718266 GSM2867548 RNA-Seq TRANSCRIPTOMIC cDNA X. maculatus at varies at varies age were sacrificed for RNA isolation. Total RNA was isolated using TriReagent (Sigma Inc., St. Louis, MO, USA). Tissues from each individual fish was homogenized in Tri-reagent followed by addition of 200 μl chloroform and the samples vigorously shaken and subjected to centrifugation at 12,000 g for 15min at 4 °c. Total RNA was further purified using RNeasy mini RNA isolation kit (Qiagen, Valencia, CA, USA). Residual DNA was eliminated by performing on column DNase digestion at 37°C for 30 min. RNA integrity was assessed by gel electrophoresis (2% agarose in TAE running buffer) and total RNA concentration was determined using a spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA). RNA quality was verified on an Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to confirm that RIN scores were above 8 prior to sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols. Libraries were constructed using the Illumina TrueSeq library preparation system that employs a polyA selection Illumina HiSeq 2000 gds 302867548 GSM2867548 GEO Accession GSM2867548